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- Green, Hyatt C.; Haugland, Richard A.; Varma, Manju; Millen, Hana T.; Borchardt, Mark A.; Field, Katharine G.; Walters, William A.; Knight, R.; Sivaganesan, Mano; Kelty, Catherine A.; Shanks, Orin C.
- Applied and environmental microbiology 2014 v.80 no.10 pp. 3086-3094
- Bacteroides; DNA; accuracy; assays; databases; detection limit; feces; humans; hybridization; nucleotide sequences; quantitative polymerase chain reaction; ribosomal RNA; sewage; surface water; water pollution
- ... Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides are among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a mo ...
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