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You searched for: Journal Applied and environmental microbiology Remove constraint Journal: Applied and environmental microbiology Publication Year 2005 Remove constraint Publication Year: 2005 Source 2005 v.71 no.11 Remove constraint Source: 2005 v.71 no.11
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1. A Bile Salt-Resistant Derivative of Bifidobacterium animalis Has an Altered Fermentation Pattern When Grown on Glucose and Maltose

Author:
Ruas-Madiedo, Patricia; Hernández-Barranco, Ana; Margolles, Abelardo; Reyes-Gavilán, Clara G. de los
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6564-6570
ISSN:
0099-2240
Subject:
Bifidobacterium animalis; probiotics; bile salts; biological resistance; carbohydrate metabolism; fermentation; glucose; maltose; ethanol production; organic acids and salts
Abstract:
... The growth of Bifidobacterium animalis subsp. lactis IPLA 4549 and its derivative with acquired resistance to bile, B. animalis subsp. lactis 4549dOx, was evaluated in batch cultures with glucose or the glucose disaccharide maltose as the main carbon source. The acquisition of bile salt resistance caused a change in growth pattern for both sugars, which mainly resulted in a preferential use of mal ...
DOI:
10.1128/AEM.71.11.6564-6570.2005
PubMed:
16269682
PubMed Central:
PMC1287725

2. Ability of Shiga Toxin-Producing Escherichia coli and Salmonella spp. To Survive in a Desiccation Model System and in Dry Foods

Author:
Hiramatsu, Reiji; Matsumoto, Masakado; Sakae, Kenji; Miyazaki, Yutaka
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6657-6663
ISSN:
0099-2240
Subject:
Escherichia coli; Salmonella; food pathogens; pathogen survival; dried foods; food contamination; bacterial contamination; food storage; drying; stress tolerance; paper; food microbiology
Abstract:
... In order to determine desiccation tolerances of bacterial strains, the survival of 58 diarrheagenic strains (18 salmonellae, 35 Shiga toxin-producing Escherichia coli [STEC], and 5 shigellae) and of 15 nonpathogenic E. coli strains was determined after drying at 35°C for 24 h in paper disks. At an inoculum level of 10⁷ CFU/disk, most of the salmonellae (14/18) and the STEC strains (31/35) survived ...
DOI:
10.1128/AEM.71.11.6657-6663.2005
PubMed:
16269694
PubMed Central:
PMC1287607

3. Abundance, Activity, and Community Structure of Pelagic Methane-Oxidizing Bacteria in Temperate Lakes

Author:
Sundh, Ingvar; Bastviken, David; Tranvik, Lars J.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6746-6752
ISSN:
0099-2240
Subject:
Methylomicrobium; Methylomonas; Methylosarcina; bacterial communities; biomass; carbon; community structure; correlation; energy; fatty acids; food webs; humic substances; ice; lakes; methane; methanotrophs; nutrient content; oxidation; sediments; summer; winter
Abstract:
... The abundance and activity of methane-oxidizing bacteria (MOB) in the water column were investigated in three lakes with different contents of nutrients and humic substances. The abundance of MOB was determined by analysis of group-specific phospholipid fatty acids from type I and type II MOB, and in situ activity was measured with a ¹⁴CH₄ transformation method. The fatty acid analyses indicated t ...
DOI:
10.1128/AEM.71.11.6746-6752.2005
PubMed:
16269705
PubMed Central:
PMC1287661

4. Algal Species and Light Microenvironment in a Low-pH, Geothermal Microbial Mat Community

Author:
Ferris, M. J.; Sheehan, K. B.; Kůhl, M.; Cooksey, K.; Wigglesworth-Cooksey, B.; Harvey, R.; Henson, J. M.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7164-7171
ISSN:
0099-2240
Subject:
Algae; autotrophs; genes; microbial communities; national parks; pH; photosynthesis; polymerase chain reaction; ribosomal RNA; sequence analysis; species diversity; springs (water); streams; temperature profiles
Abstract:
... Unicellular algae are the predominant microbial mat-forming phototrophs in the extreme environments of acidic geothermal springs. The ecology of these algae is not well known because concepts of species composition are inferred from cultivated isolates and microscopic observations, methods known to provide incomplete and inaccurate assessments of species in situ. We used sequence analysis of 18S r ...
DOI:
10.1128/AEM.71.11.7164-7171.2005
PubMed:
16269755
PubMed Central:
PMC1287733

5. Alteration of Leucine Aminopeptidase from Streptomyces septatus TH-2 to Phenylalanine Aminopeptidase by Site-Directed Mutagenesis

Author:
Arima, Jiro; Uesugi, Yoshiko; Iwabuchi, Masaki; Hatanaka, Tadashi
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7229-7235
ISSN:
0099-2240
Subject:
Streptomyces; esters; leucine; leucyl aminopeptidase; mutants; phenylalanine; site-directed mutagenesis
Abstract:
... To tailor leucine aminopeptidase from Streptomyces septatus TH-2 (SSAP) to become a convenient biocatalyst, we are interested in Phe221 of SSAP, which is thought to interact with the side chain of the N-terminal residue of the substrate. By using saturation mutagenesis, the feasibility of altering the performance of SSAP was evaluated. The hydrolytic activities of 19 mutants were investigated usin ...
DOI:
10.1128/AEM.71.11.7229-7235.2005
PubMed:
16269763
PubMed Central:
PMC1287679

6. A Method Adapting Microarray Technology for Signature-Tagged Mutagenesis of Desulfovibrio desulfuricans G20 and Shewanella oneidensis MR-1 in Anaerobic Sediment Survival Experiments

Author:
Groh, Jennifer L.; Luo, Qingwei; Ballard, Jimmy D.; Krumholz, Lee R.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7064-7074
ISSN:
0099-2240
Subject:
Desulfovibrio desulfuricans; Shewanella oneidensis; bacteria; environmental factors; genes; habitats; inputs; microarray technology; mutagenesis; screening; sediments
Abstract:
... Signature-tagged mutagenesis (STM) is a powerful technique that can be used to identify genes expressed by bacteria during exposure to conditions in their natural environments. To date, there have been no reports of studies in which this approach was used to study organisms of environmental, rather than pathogenic, significance. We used a mini-Tn10 transposon-bearing plasmid, pBSL180, that efficie ...
DOI:
10.1128/AEM.71.11.7064-7074.2005
PubMed:
16269742
PubMed Central:
PMC1287673

7. Anaerobic Microbial Communities in Lake Pavin, a Unique Meromictic Lake in France

Author:
Lehours, Anne-C.; Bardot, Corinne; Thenot, Aurelie; Debroas, Didier; Fonty, Gerard
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7389-7400
ISSN:
0099-2240
Subject:
Archaea; Eubacteria; community structure; lakes; microbial communities; oligonucleotide probes; restriction fragment length polymorphism; ribosomal RNA; sulfate-reducing bacteria; France
Abstract:
... The Bacteria and Archaea from the meromictic Lake Pavin were analyzed in samples collected along a vertical profile in the anoxic monimolimnion and were compared to those in samples from the oxic mixolimnion. Nine targeted 16S rRNA oligonucleotide probes were used to assess the distribution of Bacteria and Archaea and to investigate the in situ occurrence of sulfate-reducing bacteria and methane-p ...
DOI:
10.1128/AEM.71.11.7389-7400.2005
PubMed:
16269781
PubMed Central:
PMC1287608

8. Antagonists of Hsp16.3, a Low-Molecular-Weight Mycobacterial Chaperone and Virulence Factor, Derived from Phage-Displayed Peptide Libraries

Author:
Saha, Abhik; Sharma, Archna; Dhar, Amlanjyoti; Bhattacharyya, Bhabatarak; Roy, Siddhartha; Das Gupta, Sujoy K.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7334-7344
ISSN:
0099-2240
Subject:
Mycobacterium tuberculosis; antagonists; bacteriophages; binding proteins; clones; drug therapy; drugs; eye lens; heat shock proteins; histidine; nuclear magnetic resonance spectroscopy; pathogens; peptide libraries; synthetic peptides; tuberculosis; virulence
Abstract:
... The persistence of Mycobacterium tuberculosis is a major cause of concern in tuberculosis (TB) therapy. In the persistent mode the pathogen can resist drug therapy, allowing the possibility of reactivation of the disease. Several protein factors have been identified that contribute to persistence, one of them being the 16-kDa low-molecular-weight mycobacterial heat shock protein Hsp16.3, a homolog ...
DOI:
10.1128/AEM.71.11.7334-7344.2005
PubMed:
16269776
PubMed Central:
PMC1287729

9. Antimicrobial Resistance and Virulence Genes of Escherichia coli Isolates from Swine in Ontario

Author:
Boerlin, Patrick; Travis, Rebeccah; Gyles, Carlton L.; Reid-Smith, Richard; Janecko, Nicol; Lim, Heather; Nicholson, Vivian; McEwen, Scott A.; Friendship, Robert; Archambault, Marie
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6753-6761
ISSN:
0099-2240
Subject:
Escherichia coli; strains; isolation; swine; diarrhea; Escherichia infections; virulence; genes; antibiotic resistance; drug resistance; tetracycline; streptomycin; apramycin; sulfonamides; polymerase chain reaction; enterotoxigenic Escherichia coli; Ontario
Abstract:
... A total of 318 Escherichia coli isolates obtained from diarrheic and healthy pigs in Ontario from 2001 to 2003 were examined for their susceptibility to 19 antimicrobial agents. They were tested by PCR for the presence of resistance genes for tetracycline, streptomycin, sulfonamides, and apramycin and of 12 common virulence genes of porcine E. coli. Antimicrobial resistance frequency among E. coli ...
DOI:
10.1128/AEM.71.11.6753-6761.2005
PubMed:
16269706
PubMed Central:
PMC1287655

10. Application of Measurements of Transepithelial Electrical Resistance of Intestinal Epithelial Cell Monolayers To Evaluate Probiotic Activity

Author:
Klingberg, Trine Danø; Pedersen, Maja Herold; Cencic, Avrelija; Budde, Birgitte Bjørn
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7528-7530
ISSN:
0099-2240
Subject:
Lactobacillus plantarum; Lactobacillus salivarius; Lactobacillus; probiotics; intestinal mucosa; cell lines; electrical resistance; membrane permeability; Listeria monocytogenes; pathogenicity
Abstract:
... Among five potentially probiotic lactobacilli investigated, Lactobacillus plantarum MF1298 and Lactobacillus salivarius DC5 showed the highest increase in the transepithelial electrical resistance (TER) of polarized monolayers of Caco-2 cells, and this increase was shown to be dose dependent. Furthermore, preincubation with MF1298 attenuated a decrease in TER induced by Listeria monocytogenes. ...
DOI:
10.1128/AEM.71.11.7528-7530.2005
PubMed:
16269795
PubMed Central:
PMC1287686

11. A Propionate-Inducible Expression System for Enteric Bacteria

Author:
Lee, Sung Kuk; Keasling, Jay D.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6856-6862
ISSN:
0099-2240
Subject:
Escherichia coli; gene expression; genes; glucose; green fluorescent protein; intestinal microorganisms; propionates
Abstract:
... A series of new expression vectors (pPro) have been constructed for the regulated expression of genes in Escherichia coli. The pPro vectors contain the prpBCDE promoter (P[subscript prpB]) responsible for expression of the propionate catabolic genes (prpBCDE) and prpR encoding the positive regulator of this promoter. The efficiency and regulatory properties of the prpR-P[subscript prpB] system wer ...
DOI:
10.1128/AEM.71.11.6856-6862.2005
PubMed:
16269719
PubMed Central:
PMC1287719

12. Ascending Migration of Endophytic Rhizobia, from Roots to Leaves, inside Rice Plants and Assessment of Benefits to Rice Growth Physiology

Author:
Chi, Feng; Shen, Shi-Hua; Cheng, Hai-Ping; Jing, Yu-Xiang; Yanni, Youssef G.; Dazzo, Frank B.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7271-7278
ISSN:
0099-2240
Subject:
Oryza sativa; rice; Rhizobium; Rhizobiaceae; endophytes; translocation (plant physiology); roots; leaves; gene expression; transgenes; green fluorescent protein; bacterial colonization; shoots; dry matter accumulation; plant growth; photosynthesis; stomatal conductance; transpiration; water use efficiency; gibberellins; indole acetic acid
Abstract:
... Rhizobia, the root-nodule endosymbionts of leguminous plants, also form natural endophytic associations with roots of important cereal plants. Despite its widespread occurrence, much remains unknown about colonization of cereals by rhizobia. We examined the infection, dissemination, and colonization of healthy rice plant tissues by four species of gfp-tagged rhizobia and their influence on the gro ...
DOI:
10.1128/AEM.71.11.7271-7278.2005
PubMed:
16269768
PubMed Central:
PMC1287620

13. Bacteria Associated with Spores of the Arbuscular Mycorrhizal Fungi Glomus geosporum and Glomus constrictum

Author:
Roesti, David; Ineichen, Kurt; Braissant, Olivier; Redecker, Dirk; Wiemken, Andres; Aragno, Michel
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6673-6679
ISSN:
0099-2240
Subject:
Glomus; fungal spores; vesicular arbuscular mycorrhizae; bacterial colonization; soil bacteria; community structure; polymerase chain reaction; gel electrophoresis; ribosomal RNA; genes; nucleotide sequences; species diversity; host plants; Plantago lanceolata; Hieracium pilosella; denaturing gradient gel electrophoresis
Abstract:
... Spores of the arbuscular mycorrhizal fungi (AMF) Glomus geosporum and Glomus constrictum were harvested from single-spore-derived pot cultures with either Plantago lanceolata or Hieracium pilosella as host plants. PCR-denaturing gradient gel electrophoresis analysis revealed that the bacterial communities associated with the spores depended more on AMF than host plant identity. The composition of ...
DOI:
10.1128/AEM.71.11.6673-6679.2005
PubMed:
16269696
PubMed Central:
PMC1287740

14. Bacterial Community Dynamics and Polycyclic Aromatic Hydrocarbon Degradation during Bioremediation of Heavily Creosote-Contaminated Soil

Author:
Viñas, Marc; Sabaté, Jordi; Espuny, María José; Solanas, Anna M.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7008-7018
ISSN:
0099-2240
Subject:
hydrocarbons; petroleum; soil pollution; polluted soils; creosote; polycyclic aromatic hydrocarbons; biodegradation; bioremediation; soil bacteria; community structure; community ecology; population dynamics; population density; aeration; soil water content; nutrient availability; ribosomal RNA; genes; gel electrophoresis; nucleotide sequences; denaturing gradient gel electrophoresis; Spain
Abstract:
... Bacterial community dynamics and biodegradation processes were examined in a highly creosote-contaminated soil undergoing a range of laboratory-based bioremediation treatments. The dynamics of the eubacterial community, the number of heterotrophs and polycyclic aromatic hydrocarbon (PAH) degraders, and the total petroleum hydrocarbon (TPH) and PAH concentrations were monitored during the bioremedi ...
DOI:
10.1128/AEM.71.11.7008-7018.2005
PubMed:
16269736
PubMed Central:
PMC1287751

15. Bacterial Populations Active in Metabolism of C₁ Compounds in the Sediment of Lake Washington, a Freshwater Lake

Author:
Nercessian, Olivier; Noyes, Emma; Kalyuzhnaya, Marina G.; Lidstrom, Mary E.; Chistoserdova, Ludmila
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6885-6899
ISSN:
0099-2240
Subject:
lakes; metabolism; sediments; Washington
Abstract:
... with special emphasis on C₁ utilizers, were identified byemploying two complementary culture-independent approaches: reverse ...
DOI:
10.1128/AEM.71.11.6885-6899.2005
PubMed:
16269723
PubMed Central:
PMC1287692

16. Bactericidal Actions of a Silver Ion Solution on Escherichia coli, Studied by Energy-Filtering Transmission Electron Microscopy and Proteomic Analysis

Author:
Yamanaka, Mikihiro; Hara, Keita; Kudo, Jun
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7589-7593
ISSN:
0099-2240
Subject:
Escherichia coli; adenosine triphosphate; cell membranes; desorption; electrophoresis; enzyme inhibition; enzymes; ions; lasers; mass spectrometry; microorganisms; protein synthesis; ribosomal proteins; silver; transmission electron microscopy
Abstract:
... Bactericidal actions of the silver ion on Escherichia coli as a model microorganism were studied using energy-filtering transmission electron microscopy (EFTEM), two-dimensional electrophoresis (2-DE), and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). EFTEM observations demonstrated that the silver ion readily infiltrates the interior of E. coli, cont ...
DOI:
10.1128/AEM.71.11.7589-7593.2005
PubMed:
16269810
PubMed Central:
PMC1287701

17. Bacteriocin (Mutacin) Production by Streptococcus mutans Genome Sequence Reference Strain UA159: Elucidation of the Antimicrobial Repertoire by Genetic Dissection

Author:
Hale, John D. F.; Ting, Yi-Tian; Jack, Ralph W.; Tagg, John R.; Heng, Nicholas C. K.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7613-7617
ISSN:
0099-2240
Subject:
Streptococcus mutans; genome; loci
Abstract:
... Streptococcus mutans UA159, the genome sequence reference strain, exhibits nonlantibiotic mutacin activity. In this study, bioinformatic and mutational analyses were employed to demonstrate that the antimicrobial repertoire of strain UA159 includes mutacin IV (specified by the nlm locus) and a newly identified bacteriocin, mutacin V (encoded by SMU.1914c). ...
DOI:
10.1128/AEM.71.11.7613-7617.2005
PubMed:
16269816
PubMed Central:
PMC1287737

18. Bacteriophage Therapy To Reduce Campylobacter jejuni Colonization of Broiler Chickens

Author:
Loc Carrillo, C.; Atterbury, R. J.; El-Shibiny, A.; Connerton, P. L.; Dillon, E.; Scott, A.; Connerton, I. F.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6554-6563
ISSN:
0099-2240
Subject:
broiler chickens; bacterial colonization; Campylobacter jejuni; antibacterial properties; infection; bacteriophages; oral administration; population density; cecum; foodborne illness; disease control; human food chain
Abstract:
... Colonization of broiler chickens by the enteric pathogen Campylobacter jejuni is widespread and difficult to prevent. Bacteriophage therapy is one possible means by which this colonization could be controlled, thus limiting the entry of campylobacters into the human food chain. Prior to evaluating the efficacy of phage therapy, experimental models of Campylobacter colonization of broiler chickens ...
DOI:
10.1128/AEM.71.11.6554-6563.2005
PubMed:
16269681
PubMed Central:
PMC1287621

19. Biocatalytic Conversion of Avermectin to 4"-Oxo-Avermectin: Characterization of Biocatalytically Active Bacterial Strains and of Cytochrome P450 Monooxygenase Enzymes and Their Genes

Author:
Jungmann, Volker; Molnár, István; Hammer, Philip E.; Hill, D. Steven; Zirkle, Ross; Buckel, Thomas G.; Buckel, Dagmar; Ligon, James M.; Pachlatko, J. Paul
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6968-6976
ISSN:
0099-2240
Subject:
avermectins; oxidation; biotransformation; cytochrome P-450; unspecific monooxygenase; enzyme activity; Streptomyces tubercidicus; Streptomyces; genes; nucleotide sequences; amino acid sequences; gene overexpression; Escherichia coli
Abstract:
... 4"-Oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin. Seventeen biocatalytically active Streptomyces strains with the ability to oxidize avermectin to 4"-oxo-avermectin in a regioselective manner have been discovered in a screen of 3,334 microorganisms. The enzymes responsible for this oxidatio ...
DOI:
10.1128/AEM.71.11.6968-6976.2005
PubMed:
16269732
PubMed Central:
PMC1287622

20. Biocatalytic Conversion of Avermectin to 4"-Oxo-Avermectin: Heterologous Expression of the ema1 Cytochrome P450 Monooxygenase

Author:
Molnár, István; Hill, D. Steven; Zirkle, Ross; Hammer, Philip E.; Gross, Frank; Buckel, Thomas G.; Jungmann, Volker; Pachlatko, Johannes Paul; Ligon, James M.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6977-6985
ISSN:
0099-2240
Subject:
avermectins; oxidation; biotransformation; cytochrome P-450; unspecific monooxygenase; enzyme activity; Streptomyces tubercidicus; genes; nucleotide sequences; amino acid sequences; gene overexpression; Pseudomonas putida; Streptomyces lividans; Streptomyces avermitilis; gene transfer; genetically engineered microorganisms; oxidoreductases
Abstract:
... The cytochrome P450 monooxygenase Ema1 from Streptomyces tubercidicus R-922 and its homologs from closely related Streptomyces strains are able to catalyze the regioselective oxidation of avermectin into 4"-oxo-avermectin, a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate (V. Jungmann, I. Molnár, P. E. Hammer, D. S. Hill, R. Zirkle, T. G. Buckel, ...
DOI:
10.1128/AEM.71.11.6977-6985.2005
PubMed:
16269733
PubMed Central:
PMC1287623