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You searched for: Journal name Applied and environmental microbiology Remove constraint Journal name: Applied and environmental microbiology Publication year rev 7995-2005 Remove constraint Publication year rev: 7995-2005 Source 2005 v.71 no.11 Remove constraint Source: 2005 v.71 no.11
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1. Abundance, Activity, and Community Structure of Pelagic Methane-Oxidizing Bacteria in Temperate Lakes

Author:
Sundh, Ingvar; Bastviken, David; Tranvik, Lars J.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6746-6752
ISSN:
0099-2240
Subject:
Methylomicrobium; Methylomonas; Methylosarcina; bacterial communities; biomass; carbon; community structure; correlation; energy; fatty acids; food webs; humic substances; ice; lakes; methane; methanotrophs; nutrient content; oxidation; sediments; summer; winter
Abstract:
... The abundance and activity of methane-oxidizing bacteria (MOB) in the water column were investigated in three lakes with different contents of nutrients and humic substances. The abundance of MOB was determined by analysis of group-specific phospholipid fatty acids from type I and type II MOB, and in situ activity was measured with a ¹⁴CH₄ transformation method. The fatty acid analyses indicated t ...
DOI:
10.1128/AEM.71.11.6746-6752.2005
PubMed:
16269705
PubMed Central:
PMC1287661

2. Algal Species and Light Microenvironment in a Low-pH, Geothermal Microbial Mat Community

Author:
Ferris, M. J.; Sheehan, K. B.; Kůhl, M.; Cooksey, K.; Wigglesworth-Cooksey, B.; Harvey, R.; Henson, J. M.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7164-7171
ISSN:
0099-2240
Subject:
sequence analysis; national parks; ribosomal RNA; temperature profiles; microbial communities; springs (water); polymerase chain reaction; streams; pH; genes; species diversity; photosynthesis; autotrophs
Abstract:
... Unicellular algae are the predominant microbial mat-forming phototrophs in the extreme environments of acidic geothermal springs. The ecology of these algae is not well known because concepts of species composition are inferred from cultivated isolates and microscopic observations, methods known to provide incomplete and inaccurate assessments of species in situ. We used sequence analysis of 18S r ...
DOI:
10.1128/AEM.71.11.7164-7171.2005
PubMed:
16269755
PubMed Central:
PMC1287733

3. Antagonists of Hsp16.3, a Low-Molecular-Weight Mycobacterial Chaperone and Virulence Factor, Derived from Phage-Displayed Peptide Libraries

Author:
Saha, Abhik; Sharma, Archna; Dhar, Amlanjyoti; Bhattacharyya, Bhabatarak; Roy, Siddhartha; Das Gupta, Sujoy K.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7334-7344
ISSN:
0099-2240
Subject:
Mycobacterium tuberculosis; antagonists; bacteriophages; binding proteins; clones; drug therapy; drugs; eye lens; heat shock proteins; histidine; nuclear magnetic resonance spectroscopy; pathogens; peptide libraries; synthetic peptides; tuberculosis; virulence
Abstract:
... The persistence of Mycobacterium tuberculosis is a major cause of concern in tuberculosis (TB) therapy. In the persistent mode the pathogen can resist drug therapy, allowing the possibility of reactivation of the disease. Several protein factors have been identified that contribute to persistence, one of them being the 16-kDa low-molecular-weight mycobacterial heat shock protein Hsp16.3, a homolog ...
DOI:
10.1128/AEM.71.11.7334-7344.2005
PubMed:
16269776
PubMed Central:
PMC1287729

4. Ascending Migration of Endophytic Rhizobia, from Roots to Leaves, inside Rice Plants and Assessment of Benefits to Rice Growth Physiology

Author:
Chi, Feng; Shen, Shi-Hua; Cheng, Hai-Ping; Jing, Yu-Xiang; Yanni, Youssef G.; Dazzo, Frank B.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7271-7278
ISSN:
0099-2240
Subject:
rice; Oryza sativa; roots; shoots; endophytes; stomatal conductance; dry matter accumulation; gene expression; photosynthesis; leaves; plant growth; translocation (plant physiology); gibberellins; Rhizobium; indole acetic acid; green fluorescent protein; bacterial colonization; transgenes; water use efficiency
Abstract:
... Rhizobia, the root-nodule endosymbionts of leguminous plants, also form natural endophytic associations with roots of important cereal plants. Despite its widespread occurrence, much remains unknown about colonization of cereals by rhizobia. We examined the infection, dissemination, and colonization of healthy rice plant tissues by four species of gfp-tagged rhizobia and their influence on the gro ...
DOI:
10.1128/AEM.71.11.7271-7278.2005
PubMed:
16269768
PubMed Central:
PMC1287620

5. Bacterial Populations Active in Metabolism of C₁ Compounds in the Sediment of Lake Washington, a Freshwater Lake

Author:
Nercessian, Olivier; Noyes, Emma; Kalyuzhnaya, Marina G.; Lidstrom, Mary E.; Chistoserdova, Ludmila
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6885-6899
ISSN:
0099-2240
Subject:
sediments; metabolism; lakes
Abstract:
... with special emphasis on C₁ utilizers, were identified byemploying two complementary culture-independent approaches: reverse ...
DOI:
10.1128/AEM.71.11.6885-6899.2005
PubMed:
16269723
PubMed Central:
PMC1287692

6. Bactericidal Actions of a Silver Ion Solution on Escherichia coli, Studied by Energy-Filtering Transmission Electron Microscopy and Proteomic Analysis

Author:
Yamanaka, Mikihiro; Hara, Keita; Kudo, Jun
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7589-7593
ISSN:
0099-2240
Subject:
Escherichia coli; adenosine triphosphate; cell membranes; desorption; electrophoresis; enzyme inhibition; enzymes; ions; lasers; mass spectrometry; microorganisms; protein synthesis; ribosomal proteins; silver; transmission electron microscopy
Abstract:
... Bactericidal actions of the silver ion on Escherichia coli as a model microorganism were studied using energy-filtering transmission electron microscopy (EFTEM), two-dimensional electrophoresis (2-DE), and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). EFTEM observations demonstrated that the silver ion readily infiltrates the interior of E. coli, cont ...
DOI:
10.1128/AEM.71.11.7589-7593.2005
PubMed:
16269810
PubMed Central:
PMC1287701

7. Bacteriophage Therapy To Reduce Campylobacter jejuni Colonization of Broiler Chickens

Author:
Loc Carrillo, C.; Atterbury, R. J.; El-Shibiny, A.; Connerton, P. L.; Dillon, E.; Scott, A.; Connerton, I. F.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6554-6563
ISSN:
0099-2240
Subject:
broiler chickens; bacterial colonization; Campylobacter jejuni; antibacterial properties; infection; bacteriophages; oral administration; population density; cecum; foodborne illness; disease control; human food chain
Abstract:
... Colonization of broiler chickens by the enteric pathogen Campylobacter jejuni is widespread and difficult to prevent. Bacteriophage therapy is one possible means by which this colonization could be controlled, thus limiting the entry of campylobacters into the human food chain. Prior to evaluating the efficacy of phage therapy, experimental models of Campylobacter colonization of broiler chickens ...
DOI:
10.1128/AEM.71.11.6554-6563.2005
PubMed:
16269681
PubMed Central:
PMC1287621

8. Cloning, Biochemical Properties, and Distribution of Mycobacterial Haloalkane Dehalogenases

Author:
Jesenská, Andrea; Pavlová, Martina; Strouhal, Michal; Chaloupková, Radka; Těšínská, Iva; Monincová, Marta; Prokop, Zbyněk; Bartoš, Milan; Pavlík, Ivo; Rychlík, Ivan; Möbius, Petra; Nagata, Yuji; Damborský, Jiři
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6736-6745
ISSN:
0099-2240
Subject:
human diseases; heat stability; enzymes; enzyme activity; genes; Mycobacterium avium complex; nucleotide sequences; mycobacterial diseases; strains; Mycobacterium tuberculosis complex; animal diseases
Abstract:
... Haloalkane dehalogenases are enzymes that catalyze the cleavage of the carbon-halogen bond by a hydrolytic mechanism. Genomes of Mycobacterium tuberculosis and M. bovis contain at least two open reading frames coding for the polypeptides showing a high sequence similarity with biochemically characterized haloalkane dehalogenases. We describe here the cloning of the haloalkane dehalogenase genes dm ...
DOI:
10.1128/AEM.71.11.6736-6745.2005
PubMed:
16269704
PubMed Central:
PMC1287712

9. Comparison of the Levels of Heat Resistance of Wild-Type, cpe Knockout, and cpe Plasmid-Cured Clostridium perfringens Type A Strains

Author:
Raju, Deepa; Sarker, Mahfuzur R.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7618-7620
ISSN:
0099-2240
Subject:
Clostridium perfringens; food pathogens; heat tolerance; plasmid curing; plasmids; genes; enterotoxins; biosynthesis; knockout mutants; bacterial spores; vegetative cells
Abstract:
... An enterotoxin (cpe) plasmid was cured from a Clostridium perfringens non-food-borne gastrointestinal disease (NFBGID) isolate, and the heat resistance levels of wild-type, cpe knockout, and cpe plasmid-cured strains were compared. Our results demonstrated that (i) wild-type cpe has no influence in mediating high-level heat resistance in C. perfringens and (ii) the cpe plasmid does not confer heat ...
DOI:
10.1128/AEM.71.11.7618-7620.2005
PubMed:
16269817
PubMed Central:
PMC1287616

10. Copper-Adapted Suillus luteus, a Symbiotic Solution for Pines Colonizing Cu Mine Spoils

Author:
Adriaensen, K.; Vrålstad, T.; Noben, J.-P.; Vangronsveld, J.; Colpaert, J. V.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7279-7284
ISSN:
0099-2240
Subject:
ectomycorrhizae; soil pollution; copper; zinc; seedlings; nutrient uptake; phytoremediation; land restoration; Pinus sylvestris; root growth; Suillus luteus; metal tolerance; polluted soils; mine spoil; antifungal properties; Norway
Abstract:
... Natural populations thriving in heavy-metal-contaminated ecosystems are often subjected to selective pressures for increased resistance to toxic metals. In the present study we describe a population of the ectomycorrhizal fungus Suillus luteus that colonized a toxic Cu mine spoil in Norway. We hypothesized that this population had developed adaptive Cu tolerance and was able to protect pine trees ...
DOI:
10.1128/AEM.71.11.7279-7284.2005
PubMed:
16269769
PubMed Central:
PMC1287625

11. CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

Author:
Sermon, Jan; Wevers, Eva M.-R. P.; Jansen, Leentje; Spiegeleer, Philipp de; Vanoirbeek, Kristof; Aertsen, Abram; Michiels, Chris W.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6515-6523
ISSN:
0099-2240
Subject:
food biopreservatives; Escherichia coli; transporters; Salmonella Typhimurium; magnesium; food microbiology; bacterial proteins; mutants; stress tolerance; nickel; enzyme activity; peroxidase; oxidative stress; antibacterial properties
Abstract:
... The enzyme lactoperoxidase is part of the innate immune system in vertebrates and owes its antimicrobial activity to the formation of oxidative reaction products from various substrates. In a previous study, we have reported that, with thiocyanate as a substrate, the lactoperoxidase system elicits a distinct stress response in Escherichia coli MG1655. This response is different from but partly ove ...
DOI:
10.1128/AEM.71.11.6515-6523.2005
PubMed:
16269676
PubMed Central:
PMC1287667

12. Crucial Role of Extracellular Polysaccharides in Desiccation and Freezing Tolerance in the Terrestrial Cyanobacterium Nostoc commune

Author:
Tamaru, Yoshiyuki; Takani, Yayoi; Yoshida, Takayuki; Sakamoto, Toshio
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7327-7333
ISSN:
0099-2240
Subject:
gas production (biological); Nostoc commune; soil microorganisms; stress tolerance; drying; freezing; cold tolerance; polysaccharides; photosynthesis; oxygen
Abstract:
... The cyanobacterium Nostoc commune is adapted to the terrestrial environment and has a cosmopolitan distribution. In this study, the role of extracellular polysaccharides (EPS) in the desiccation tolerance of photosynthesis in N. commune was examined. Although photosynthetic O₂ evolution was not detected in desiccated colonies, the ability of the cells to evolve O₂ rapidly recovered after rehydrati ...
DOI:
10.1128/AEM.71.11.7327-7333.2005
PubMed:
16269775
PubMed Central:
PMC1287664

13. Culture-Independent Techniques for Rapid Detection of Bacteria Associated with Loss of Chloramine Residual in a Drinking Water System

Author:
Hoefel, Daniel; Monis, Paul T.; Grooby, Warwick L.; Andrews, Stuart; Saint, Christopher P.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6479-6488
ISSN:
0099-2240
Subject:
Nitrosomonas cryotolerans; bacteria; decision making; denaturing gradient gel electrophoresis; disinfection; drinking water; esterases; flow cytometry; genes; industry; nucleotide sequences; polymerase chain reaction; rapid methods; ribosomal RNA; sequence analysis; water treatment
Abstract:
... Chloramination is often the disinfection regimen of choice for extended drinking water systems. However, this process is prone to instability due to the growth of nitrifying bacteria. This is the first study to use alternative approaches for rapid investigation of chloraminated drinking water system instability in which flow cytometric cell sorting of bacteria with intact membranes (membrane-intac ...
DOI:
10.1128/AEM.71.11.6479-6488.2005
PubMed:
16269672
PubMed Central:
PMC1287627

14. Detection of Carp Interstitial Nephritis and Gill Necrosis Virus in Fish Droppings

Author:
Dishon, Arnon; Perelberg, Ayana; Bishara-Shieban, Janette; Ilouze, Maya; Davidovich, Maya; Werker, Shlomit; Kotler, Moshe
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7285-7291
ISSN:
0099-2240
Subject:
vertebrate viruses; viral diseases of animals and humans; immunohistochemistry; Herpesviridae; koi; Cyprinus carpio; DNA; feces; polymerase chain reaction; viral antigens; intestines; fish diseases; microbial detection; enzyme-linked immunosorbent assay; kidneys; disease diagnosis
Abstract:
... Carp interstitial nephritis and gill necrosis virus (CNGV) is an unclassified large DNA virus that morphologically resembles members of the Herpesviridae but contains a large (ca. [approximately]280-kbp) linear double-stranded DNA. This virus has also been named koi herpesvirus, koi herpes-like virus, and cyprinid herpesvirus 3. CNGV is the cause of a lethal disease that afflicts common carp and k ...
DOI:
10.1128/AEM.71.11.7285-7291.2005
PubMed:
16269770
PubMed Central:
PMC1287658

15. Detection of Salmonella enterica in Naturally Contaminated Liquid Eggs by Loop-Mediated Isothermal Amplification, and Characterization of Salmonella Isolates

Author:
Ohtsuka, Kayoko; Yanagawa, Keiko; Takatori, Kosuke; Hara-Kudo, Yukiko
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6730-6735
ISSN:
0099-2240
Subject:
bacterial contamination; food contamination; polymerase chain reaction; food industry; food microbiology; pulsed-field gel electrophoresis; microbial detection; egg products; Salmonella enterica
Abstract:
... Loop-mediated isothermal amplification (LAMP) assay was effective in detecting Salmonella enterica in naturally contaminated liquid egg samples. Salmonella was detected in 110 samples taken from four egg-breaking plants. The egg samples were pre-enriched in buffered peptone water (BPW) at 37°C for 20 h. The selective enrichment was done in Rappaport-Vassiliadis or tetrathionate broth and plated on ...
DOI:
10.1128/AEM.71.11.6730-6735.2005
PubMed:
16269703
PubMed Central:
PMC1287618

16. Development of a Rapid Method for Simultaneous Recovery of Diverse Microbes in Drinking Water by Ultrafiltration with Sodium Polyphosphate and Surfactants

Author:
Hill, Vincent R.; Polaczyk, Amy L.; Hahn, Donghyun; Narayanan, Jothikumar; Cromeans, Theresa L.; Roberts, Jacquelin M.; Amburgey, James E.
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6878-6884
ISSN:
0099-2240
Subject:
fetal bovine serum; Cryptosporidium parvum; water pollution; Enterococcus faecalis; ultrafiltration; environmental monitoring; Salmonella Typhimurium; microorganisms; rapid methods; surfactants; tap water; sodium; Bacillus subtilis; Escherichia coli
Abstract:
... The ability to simultaneously concentrate diverse microbes is an important consideration for sample collection methods that are used for emergency response and environmental monitoring when drinking water may be contaminated with an array of unknown microbes. This study focused on developing a concentration method using ultrafilters and different combinations of a chemical dispersant (sodium polyp ...
DOI:
10.1128/AEM.71.11.6878-6884.2005
PubMed:
16269722
PubMed Central:
PMC1287739

17. Development of a cob-18S rRNA Gene Real-Time PCR Assay for Quantifying Pfiesteria shumwayae in the Natural Environment

Author:
Zhang, Huan; Lin, Senjie
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7053-7063
ISSN:
0099-2240
Subject:
Pfiesteria; oceans; seawater; polymerase chain reaction; cytochrome b; ribosomal RNA; genes; microbial detection; genetic variation; estuaries; coastal water; nucleotide sequences; Atlantic Ocean; Pacific Ocean
Abstract:
... Despite the fact that the heterotrophic dinoflagellate Pfiesteria shumwayae is an organism of high interest due to alleged toxicity, its abundance in natural environments is poorly understood. To address this inadequacy, a real-time quantitative PCR assay based on mitochondrial cytochrome b (cob) and18S rRNA gene was developed and P. shumwayae abundance was investigated in several geographic locat ...
DOI:
10.1128/AEM.71.11.7053-7063.2005
PubMed:
16269741
PubMed Central:
PMC1287735

18. Distribution, Localization, and Phylogeny of Abundant Populations of Crenarchaeota in Anaerobic Granular Sludge

Author:
Collins, Gavin; O'Connor, Leanne; Mahony, Thérèse; Gieseke, Armin; de Beer, Dirk; O'Flaherty, Vincent
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7523-7527
ISSN:
0099-2240
Subject:
Crenarchaeota; fluorescence in situ hybridization; molecular cloning; phylogeny; ribosomal RNA; sludge
Abstract:
... Eight anaerobic granular sludges were surveyed for Crenarchaeota using rRNA gene cloning. Microbial arrangement and substrate uptake patterns were elucidated by fluorescent in situ hybridization and beta imaging. Group 1.3 Crenarchaeota represented up to 50% of Archaea and 25% of the total microbiota in five sludges. Crenarchaeota were localized in close association with methanogenic ARCHAEA: ...
DOI:
10.1128/AEM.71.11.7523-7527.2005
PubMed:
16269794
PubMed Central:
PMC1287707

19. Diversity, Localization, and Physiological Properties of Filamentous Microbes Belonging to Chloroflexi Subphylum I in Mesophilic and Thermophilic Methanogenic Sludge Granules

Author:
Yamada, Takeshi; Sekiguchi, Yuji; Imachi, Hiroyuki; Kamagata, Yoichi; Ohashi, Akiyoshi; Harada, Hideki
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 7493-7503
ISSN:
0099-2240
Subject:
DNA primers; amino acids; bacteria; carbohydrates; cell biology; clones; ecology; fluorescence in situ hybridization; genes; genetic variation; granules; polymerase chain reaction; ribosomal RNA; sludge; upflow anaerobic sludge blanket reactor; wastewater
Abstract:
... We previously reported that the thermophilic filamentous anaerobe Anaerolinea thermophila, which is the first cultured representative of subphylum I of the bacterial phylum Chloroflexi, not only was one of the predominant constituents of thermophilic sludge granules but also was a causative agent of filamentous sludge bulking in a thermophilic (55°C) upflow anaerobic sludge blanket (UASB) reactor ...
DOI:
10.1128/AEM.71.11.7493-7503.2005
PubMed:
16269791
PubMed Central:
PMC1287668

20. Effect of Different Carbon Sources on Community Composition of Bacterial Enrichments from Soil

Author:
Wawrik, Boris; Kerkhof, Lee; Kukor, Jerome; Zylstra, Gerben
Source:
Applied and environmental microbiology 2005 v.71 no.11 pp. 6776-6783
ISSN:
0099-2240
Subject:
soil bacteria; community structure; soil ecology; species diversity; restriction fragment length polymorphism; nutrient availability; glucose; benzoic acid; leucine; acetic acid; enrichment culture
Abstract:
... Soil is a highly heterogeneous matrix, which can contain thousands of different bacterial species per gram. Only a small component of this diversity (maybe <1%) is commonly captured using standard isolation techniques, although indications are that a larger proportion of the soil community is in fact culturable. Better isolation techniques yielding greater bacterial diversity would be of benefit f ...
DOI:
10.1128/AEM.71.11.6776-6783.2005
PubMed:
16269709
PubMed Central:
PMC1287611