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- Bolen, P.L.; Roth, K.A.; Freer, S.N.
- Applied and environmental microbiology 1986 v.52 no.4 pp. 660
- lignocellulose; yeasts; xylose; fermentation; aldoses; xylitol; oxidoreductases; biochemistry; enzyme activity; purification; ethanol
- ... Although xylose is a major product of hydrolysis of lignocellulosic materials, few yeasts are able to convert it to ethanol. In Pachysolen tannophilus, one of the few xylose-fermenting yeasts found, aldose reductase and xylitol dehydrogenase were found to be key enzymes in the metabolic pathway for xylose fermentation. This paper presents a method for the rapid and simultaneous purification of bot ...