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- Doorn, J. van.; Hollinger, T.C.; Oudega, B.
- Applied and environmental microbiology 2001 v.67 no.2 pp. 598-607
- disease diagnosis; Xanthomonas; plant pathogenic bacteria; genes; fimbriae; bacterial proteins; nucleotide sequences; amino acid sequences; polymerase chain reaction; plant diseases and disorders; DNA; Hyacinthus; DNA primers
- ... A sensitive and specific detection method was developed for Xanthomonas hyacinthi; this method was based on amplification of a subsequence of the type IV fimbrial-subunit gene fimA from strain S148. The fimA gene was amplified by PCR with degenerate DNA primers designed by using the N-terminal and C-terminal amino acid sequences of trypsin fragments of FimA. The nucleotide sequence of fimA was det ...
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