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- Kurakake, Masahiro; Kitagawa, Yuhei; Okazaki, Atsushi; Shimizu, Kazuyuki
- Applied biochemistry and biotechnology 2017 v.183 no.4 pp. 1455-1464
- alginate lyase; sodium alginate, etc ; Paenibacillus; amino acid sequences; hyaluronic acid; molecular weight; oligosaccharides; pH; polymerization; reducing sugars; soil; temperature; Show all 12 Subjects
- ... Paenibacillus sp. S29 was isolated from soil and produces an alginate lyase. The molecular weight of this enzyme was 32 kDa and the N-terminal amino acid sequence was ASVTKST. The optimal pH was approximately 8.7 and the enzyme was stable over a pH range of 5.6 to 8.8 at 40 °C for 60 min. The optimal temperature was approximately 50 °C, and the residual activity was not decreased at temperatures o ...