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- Wang, Haiyin; Jones, Richard W.
- Applied biochemistry and biotechnology 1999 v.81 no.3 pp. 153
- Macrophomina phaseolina; endo-1,4-beta-glucanase; genes; gene transfer; gene expression; Escherichia coli; Saccharomyces cerevisiae; enzyme activity; enzymatic hydrolysis; carboxymethylcellulose
- ... Functional expression of a β-d-1,4 glucanase-encoding gene (egl1) from a filamentous fungus was achieved in both Escherichia coli and Saccharomyces cerevisiae using a modified version of pRS413. Optimal activity of the E. coli-expressed enzyme was found at incubation temperatures of 60°C, whereas the enzyme activity was optimal at 40°C when expressed by S. cerevisiae. Enzyme activity at different ...