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- Bao, Lei; Huang, Qiang; Chang, Lei; Sun, Qingwen; Zhou, Jungang; Lu, Hong
- Applied biochemistry and biotechnology 2012 v.166 no.1 pp. 72-86
- rumen; molecular models; substrate specificity; reducing sugars; xylan; enzymes; chromatography; cellulose; metagenomics; microorganisms; genes; active sites; hydrolysis; structure-activity relationships; synergism; screening; yaks; saccharification; Escherichia coli
- ... Two β-glucosidase/xylosidase genes, Rubg3A and Rubg3B, were cloned from yak rumen uncultured microorganisms by metagenome method and function-based screening. Recombinant RuBG3A and RuBG3B purified from Escherichia coli were characterized for enzymatic properties, and they exhibited activity against 4-nitrophenyl-β-D-glucopyranoside and 4-nitrophenyl-β-D-xylopyranoside, suggesting bifunctional β-g ...
- Kristiansson, Helena; Timson, David J.
- Applied biochemistry and biotechnology 2012 v.166 no.1 pp. 57-63
- active sites; catalytic activity; galactokinase; phosphorylation; substrate specificity; sugars; tyrosine
- ... N-acetylgalactosamine kinase is a member of the GHMP family of small molecule kinases which catalyses the ATP-dependent phosphorylation of N-acetylgalactosamine. It is highly similar in structure and sequence to galactokinase. Alteration of galactokinase at a key tyrosine residue (Tyr-379 in the human enzyme) has been shown to dramatically enhance the substrate range of this enzyme. Here, we inves ...