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- Bao, Lei; Huang, Qiang; Chang, Lei; Sun, Qingwen; Zhou, Jungang; Lu, Hong
- Applied biochemistry and biotechnology 2012 v.166 no.1 pp. 72-86
- rumen; molecular models; substrate specificity; reducing sugars; xylan; enzymes; chromatography; cellulose; metagenomics; microorganisms; genes; active sites; hydrolysis; structure-activity relationships; synergism; screening; yaks; saccharification; Escherichia coli
- ... Two β-glucosidase/xylosidase genes, Rubg3A and Rubg3B, were cloned from yak rumen uncultured microorganisms by metagenome method and function-based screening. Recombinant RuBG3A and RuBG3B purified from Escherichia coli were characterized for enzymatic properties, and they exhibited activity against 4-nitrophenyl-β-D-glucopyranoside and 4-nitrophenyl-β-D-xylopyranoside, suggesting bifunctional β-g ...
- Wang, Dan; Sun, Jie; Yu, Hui-Lei; Li, Chun-Xiu; Bao, Jie; Xu, Jian-He
- Applied biochemistry and biotechnology 2012 v.166 no.1 pp. 176-186
- Aspergillus fumigatus; biodegradation; cellulose; corn cobs; corn stover; endo-1,4-beta-glucanase; fermentation; proteins; saccharification; soil sampling; steam; xylanases
- ... Either the natural biodegradation process or the industrial hydrolytic process requires synergistic interactions between various cellulases. However, it is sometimes impeded by low hydrolytic rate of existing cellulases and the lack of accessory enzymes. Herein, the ability of a commercial cellulase (Spezyme CP, from Genencor) to degrade steam explosion-pretreated corn stover was significantly imp ...