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- Li, Deshan, et al. Show all 6 Authors
- Applied biochemistry and biotechnology 2015 v.175 no.3 pp. 1633-1643
- Escherichia coli; Western blotting; anticarcinogenic activity; biopharmaceuticals; biotechnology; cytosine deaminase; cytotoxicity; dose response; flow cytometry; green fluorescent protein; hepatoma; human cell lines; humans; in vitro studies; in vivo studies; liver; mice; microscopy; mutants; permeability; site-directed mutagenesis; therapeutics; thermal stability
- ... Gene-directed enzyme-prodrug therapy is a method whereby cancerous tumors are selectively eradicated with minimal impact to healthy tissue. Due to its thermostability, E. coli cytosine deaminase (bCD) is one of the most widely used enzyme-prodrug combinations. However, wild-type bCD (wtbCD) displays a relatively poor turnover of 5-fluorocytosine (5-FC), and also has low permeability as a hexamer m ...