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- Lin, Jianping, et al. Show all 3 Authors
- Applied biochemistry and biotechnology 2010 v.160 no.2 pp. 456-466
- succinic acid; fermenters; glucose; Agrobacterium radiobacter; aminolevulinic acid; gene expression; codons; microbial growth; genetic engineering; glycine (amino acid); batch fermentation; Escherichia coli
- ... The 5-aminolevulinate (ALA) synthase gene (hemA) from Agrobacterium radiobacter zju-0121, which was cloned previously in our laboratory, contains several rare codons. To enhance the expression of this gene, Escherichia coli Rosetta(DE3), which is a rare codon optimizer strain, was picked out as the host to construct an efficient recombinant strain. Cell extracts of the recombinant E. coli were ana ...