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You searched for: Journal Applied biochemistry and microbiology Remove constraint Journal: Applied biochemistry and microbiology Publication Year 2013 Remove constraint Publication Year: 2013 Source 2013 v.49 no.1 Remove constraint Source: 2013 v.49 no.1
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1. An amylase from fresh fruiting bodies of the monkey head mushroom Hericium Erinaceum

Author:
Du, F.; Wang, H. X.; Ng, T. B.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 23-27
ISSN:
0003-6838
Subject:
Hericium; ammonium acetate; enzyme activity; filtration; fruiting bodies; gels; ion exchange chromatography; ions; iron; manganese; mercury; molecular weight; monkeys; mushrooms; pH; polyacrylamide gel electrophoresis; sodium chloride
Abstract:
... An amylase with a molecular mass of 55 kDa and an N-terminal sequence exhibiting similarity to enzyme from Bacteroides thetaitaomicron was isolated from fruiting bodies of the monkey head mushroom Hericium erinaceum. The purification scheme included extraction with distilled water, ion exchange chromatography on DEAE-cellulose and SP-sepharose, and gel filtration by FPLC on Superdex 75. The amylas ...
DOI:
10.1134/S0003683813010043

2. Antiradical properties of oregano, thyme, and savory essential oils

Author:
Alinkina, E. S.; Misharina, T. A.; Fatkullina, L. D.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 73-78
ISSN:
0003-6838
Subject:
Origanum vulgare; Satureja hortensis; essential oils; oregano; savory; thyme
Abstract:
... In model reactions with the stable free 2,2-diphenyl-1-picrylhydrazyl radical, the antiradical properties of essential oils of thyme (Thymus vulgare), oregano (Origanum vulgare), and savory (Satureja hortensis) that are similar in the qualitative composition, but differ in the quantitative content of the main components, were studied and compared with the properties of synthetic antioxidant ionol. ...
DOI:
10.1134/S000368381301002X

3. Biochemical markers of virus cytopathogenicity in macrophages

Author:
Plekhova, N. G.; Somova, L. M.; Krylova, N. V.; Leonova, G. N.; Lyapun, I. N.; Smirnov, I. S.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 64-72
ISSN:
0003-6838
Subject:
biomarkers; cytopathogenicity; macrophages; metabolism; virulence; viruses
Abstract:
... The results of macrophage metabolism studies at their infection by viruses differing in the level of virulence are presented. With the purpose of optimizing the estimation of viral cytopathogenic effects on macrophages, an index of cell reactions, which allows one to reveal the degree of virus influence in standard units, is offered. Generally, the application of high-sensitivity methods for funct ...
DOI:
10.1134/S0003683813010158

4. Biodiesel fuel production from lipids of filamentous fungi

Author:
Lunin, V. V.; Sergeeva, Ya. E.; Galanina, L. A.; Mysyakina, I. S.; Ivashechkin, A. A.; Bogdan, V. I.; Feofilova, E. P.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 46-52
ISSN:
0003-6838
Subject:
Cunninghamellaceae; biodiesel; biomass; biotechnology; carbon; fuel production; fungi; industrial wastes; inoculum; lipid content; mycelium; nitrogen; spores; triacylglycerols
Abstract:
... The main stages in the production of biodiesel fuel from lipids of filamentous fungi belonging to the order Mucorales are described. Fungi of the family Cunninghamellaceae have been screened; the lipogenic activity of the examined strains has been assessed; and a producer generating up to 50% of lipids, represented by triacylglycerols, has been found. The substitution effect of a source of carbon ...
DOI:
10.1134/S0003683813010122

5. Crystal formation peculiarities in pigmented cultures of Bacillus thuringiensis

Author:
Chil-Hakobyan, L. A.; Hambardzumyan, A. A.; Chakhalyan, A. Kh.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 34-40
ISSN:
0003-6838
Subject:
Bacillus thuringiensis; clones; crystals; insecticides; pigmentation; serotypes; toxins
Abstract:
... A direct correlation has been established between pink-colored pigmentation and the production of insecticide crystals (toxins) for some Bacillus thuringiensis (BT) pigmented cultures. This regularity was for the first time determined by us for BT strains of the H3, H10, and H16 serotype. Pigment-free clones of these serotypes do not produce crystals. A correlation was not observed in the case of ...
DOI:
10.1134/S0003683813010031

6. Development of multiplex PCR for fast detection of Paenibacillus Larvae in putrid masses and in isolated bacterial colonies

Author:
Rusenova, N. V.; Parvanov, P.; Stanilova, S.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 79-84
ISSN:
0003-6838
Subject:
American foul brood; Apoidea; DNA; Paenibacillus larvae; centrifugation; genes; heat; polymerase chain reaction; ribosomal RNA
Abstract:
... The present study was performed to develop a fast and sensitive multiplex polymerase chain reaction protocol for routine diagnostics of American foulbrood. A new approach for detection of Paenibacillus larvae in putrid masses was described. Forty five samples of putrid masses obtained from bee combs suspicious for American foulbrood, a reference strain Paenibacillus larvae (NBIMCC 8478), clinical ...
DOI:
10.1134/S0003683813010171

7. Effect of cyclopentene β,β′-triketone disodium salt on the activity of hydrolases and the state of tobacco mosaic virus particles in tobacco leaves

Author:
Lapshina, L. A.; Reunov, A. V.; Nagorskaya, V. P.; Shestak, O. P.; Novikov, V. L.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 59-63
ISSN:
0003-6838
Subject:
Tobacco mosaic virus; defense mechanisms; leaves; proteinases; ribonucleases; tobacco; viruses
Abstract:
... The activity of hydrolases (protease, RNase) in uninfected and tobacco mosaic virus-infected tobacco leaves of the Samsun variety, untreated and treated with disodium salt of 2-acetyl-4-hydroxycarbonyl-methylthio-5-chlorocyclopent-4-en-1,3-dione (DS), was determined. It was shown that treatment of leaves with this compound significantly increased the activity of hydrolases in them compared to untr ...
DOI:
10.1134/S0003683813010110

8. Effect of plant stilbene precursors on the biosynthesis of resveratrol in Vitis amurensis Rupr. Cell cultures

Author:
Kiselev, K. V.; Shumakova, O. A.; Manyakhin, A. Yu.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 53-58
ISSN:
0003-6838
Subject:
Vitis amurensis; biosynthesis; cell culture; genes; phenylalanine; resveratrol
Abstract:
... The biosynthesis of resveratrol after the application of a precursor for biosynthesis, i.e., phenylalanine (Phe), has been studied. The application of Phe has been shown to increase significantly the expression of the phenylalanine-ammonia-lyase (PAL) and stilbene synthase (STS) genes and enhance the production of resveratrol by 8.5 times. Data on resveratrol production after the addition of Phe a ...
DOI:
10.1134/S0003683813010079

9. Fungi of the genus Penicillium as producers of physiologically active compounds (Review)

Author:
Kozlovskii, A. G.; Zhelifonova, V. P.; Antipova, T. V.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 1-10
ISSN:
0003-6838
Subject:
Penicillium; benzodiazepines; biosynthesis; diketopiperazines; ergot alkaloids; excretion; fungi; physicochemical properties; quinolines; secondary metabolites; taxonomy; tryptophan
Abstract:
... Fungi of the genus Penicillium isolated from little studied habitats are able to synthesize both previously known and new physiologically active compounds with diverse structures. They include secondary metabolites of alkaloid nature, i.e., ergot alkaloids, diketopiperazines, quinolines, quinazolines, benzodiazepines, and polyketides. We discuss the use of profiles of secondary metabolites for tax ...
DOI:
10.1134/S0003683813010092

10. β-Glucan content and hydration properties of filamentous fungi

Author:
Kyanko, M. V.; Canel, R. S.; Ludemann, V.; Pose, G.; Wagner, J. R.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 41-45
ISSN:
0003-6838
Subject:
Basidiomycota; Paecilomyces variotii; Penicillium; absorption; beta-glucans; dietary fiber; mycelium; water holding capacity; yeasts
Abstract:
... The aim of this work was to isolate and identify filamentous fungi from several sources to study the dietary fiber and β-glucan content. The fungal hydration properties such as water absorption and water holding capacities were also evaluated. Total dietary fiber of isolates exhibited a noticeable variability from 16 to 53% and the highest values were obtained for the genera Paecilomyces and Penic ...
DOI:
10.1134/S0003683813010080

11. Heterologous expression, purification, and properties of a chymotrypsin inhibitor isolated from potatoes

Author:
Parfenov, I. A.; Revina, T. A.; Gerasimova, N. G.; Kladnitskaya, G. V.; Valueva, T. A.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 28-33
ISSN:
0003-6838
Subject:
Escherichia coli; Kunitz-type proteinase inhibitor; Solanum tuberosum; chymotrypsin; growth retardation; high performance liquid chromatography; inclusion bodies; potatoes
Abstract:
... The PKPIJ-B gene encoding a chymotrypsin inhibitor from a subfamily of potato Kunitz-type proteinase inhibitors (PKPI) in potatoes (Solanum tuberosum L. cv. Yubilei Zhukova) was cloned into a pET23a vector and then expressed in Escherichia coli. The recombinant PKPIJ-B protein obtained in the inclusion bodies was denatured, purified by high-performance liquid chromatography (HPLC) on Mono Q under ...
DOI:
10.1134/S0003683813010146

12. Physicochemical properties and antiproliferative activity of recombinant Yersinia pseudotuberculosis L-asparaginase

Author:
Pokrovskii, V. S.; Pokrovskaya, M. V.; Aleksandrova, S. S.; Anrianov, R. M.; Zhdanov, D. D.; Osmel’yanyuk, N. M.; Treshchalina, E. M.; Sokolov, N. N.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 18-22
ISSN:
0003-6838
Subject:
Escherichia coli; Yersinia pseudotuberculosis; pH; temperature
Abstract:
... The physicochemical, catalytic, and antiproliferative activity of a recombinant L-asparaginase from Yersinia pseudotuberculosis (YpA) have been studied. The following results were obtained: the K M value for L-asparagine is 17 ± 0.9 μM, the optimal temperature is 60°C, pH is 8.0, pI is 5.4 ± 0.3, the L-glutaminase activity is no more than 5–6% of the L-asparaginase activity, and the antiproliferat ...
DOI:
10.1134/S000368381301016X

13. Refolding of recombinant human interferon α-2a from Escherichia coli by urea gradient size exclusion chromatography

Author:
Gao, F.; Shi, L.; Xu, L. -X.
Source:
Applied biochemistry and microbiology 2013 v.49 no.1 pp. 11-17
ISSN:
0003-6838
Subject:
glutathione; protein synthesis; humans; inclusion bodies; recombinant proteins; gel chromatography; urea; interferons; Escherichia coli
Abstract:
... Protein refolding is still a puzzle in the production of recombinant proteins expressed as inclusion bodies (IBs) in Escherichia coli. Gradient size exclusion chromatography (SEC) is a recently developed method for refolding of recombinant proteins in IBs. In this study, we used a decreasing urea gradient SEC for the refolding of recombinant human interferon α-2a (rhIFNα-2a) which was overexpresse ...
DOI:
10.1134/S0003683813010055