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- Guo, Jing; Cao, Yujin; Liu, Hui; Zhang, Rubing; Xian, Mo; Liu, Huizhou
- Applied microbiology and biotechnology 2019 v.103 no.6 pp. 2597-2608
- Escherichia coli; NAD (coenzyme); NADP (coenzyme); biocatalysts; biofuels; biosynthesis; byproducts; carbon dioxide; culture media; fermentation; glucose; glycerol; isoprene; metabolic engineering; microorganisms; recycling; volatile organic compounds
- ... The biosynthesis of isoprene by microorganisms is a promising green route. However, the yield of isoprene is limited due to the generation of excess NAD(P)H via the mevalonate (MVA) pathway, which converts more glucose into CO₂ or undesired reduced by-products. The production of 1,3-propanediol (1,3-PDO) from glycerol is a typical NAD(P)H-consuming process, which restricts 1,3-PDO yield to ~ 0.7 m ...
- Kondoh, Mariko; Hirasawa, Takashi
- Applied microbiology and biotechnology 2019 v.103 no.6 pp. 2609-2619
- Corynebacterium glutamicum; biosynthesis; byproducts; cystathionine gamma-lyase; cysteine; cystine; gene overexpression; genes; lactate dehydrogenase; lactic acid; metabolic engineering; mutants; mutation; phosphoglycerate dehydrogenase; serine
- ... L-Cysteine is a commercially important amino acid. Here, we report the construction of L-cysteine-producing Corynebacterium glutamicum using a metabolic engineering approach. L-Serine O-acetyltransferase (SAT), encoded by cysE gene, is a key enzyme of L-cysteine biosynthesis, because of its feedback inhibition by L-cysteine. Therefore, we introduced a mutation into the C. glutamicum cysE gene, whi ...