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- Chen, Wei; Zhang, Xuanxuan; Xiong, Dandan; Jin, Jian-Ming; Tang, Shuang-Yan
- Applied microbiology and biotechnology 2019 v.103 no.7 pp. 3205-3213
- DNA; Pseudomonas putida; biomarkers; biosensors; carbaryl; engineering; enzymes; mutants; pesticide residues; regulatory proteins; transcription (genetics); uric acid
- ... Transcriptional regulatory proteins (TRPs)-based whole-cell biosensors are promising owing to their specificity and sensitivity, but their applications are currently limited. Herein, TRPs were adapted for the extracellular detection of a disease biomarker, uric acid, and a typical pesticide residue, carbaryl. A mutant regulatory protein that specifically recognizes carbaryl as its non-natural effe ...