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1. Analysis and validation of the pho regulon in the tacrolimus-producer strain Streptomyces tsukubaensis: differences with the model organism Streptomyces coelicolor

Author:
Martínez-Castro, Miriam; Barreiro, Carlos; Martín, Juan F.
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7029-7045
ISSN:
0175-7598
Subject:
Streptomyces coelicolor; alkaline phosphatase; bacteria; bioinformatics; conserved sequences; gel electrophoresis; metabolism; models; nitrogen; nucleotides; phosphates; regulon; secondary metabolites; sequence analysis; tacrolimus
Abstract:
... Inorganic and organic phosphate controls both primary and secondary metabolism in Streptomyces genus. Metabolism regulation by phosphate in Streptomyces species is mediated by the PhoR-PhoP two-component system. Response regulator PhoP binds to conserved sequences of 11 nucleotides called direct repeat units (DRus), whose organization and conservation determine the binding of PhoP to distinct prom ...
DOI:
10.1007/s00253-018-9140-0

2. A Pichia pastoris single-cell biosensor for detection of enzymatically produced methanol

Author:
Takeya, Tomoyuki; Yurimoto, Hiroya; Sakai, Yasuyoshi
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7017-7027
ISSN:
0175-7598
Subject:
Pichia pastoris; biosensors; biotransformation; detection limit; enzyme activity; flow cytometry; fluorescence; fluorescence microscopy; fluorescent proteins; genes; geometry; metabolism; methanol; pectinesterase; protein content; quantitative analysis; screening; yeasts
Abstract:
... We conducted single-cell analyses of the methylotrophic yeast Pichia pastoris to develop a biosensor for the detection of methanol produced by heterologous enzymes. In this biosensor, methanol and its subsequent metabolism induce expression of a gene encoding a fluorescent protein that was placed under the control of a methanol-inducible promoter. Using quantitative analyses of fluorescence micros ...
DOI:
10.1007/s00253-018-9144-9

3. Back to the past—forever young: cutting-edge biochemical and microbiological tools for cultural heritage conservation

Author:
Mazzoli, Roberto; Giuffrida, Maria Gabriella; Pessione, Enrica
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6815-6825
ISSN:
0175-7598
Subject:
bacteria; biodegradation; corrosion; cultural heritage; discoloration; enzymes; fabrics; monuments; paper; pigmentation; pollution; weathering
Abstract:
... Ancient documents and milestones of human history such as manuscripts and textiles are fragile and during aging undergo chemical, physical, and biological deterioration. Among the different causes of damage, also human intervention plays a role since some restoration strategies proved to be transient and/or they generated further damage. Outdoor monuments undergo deterioration since they are expos ...
DOI:
10.1007/s00253-018-9121-3

4. Biocatalytic hydrogenation of the C=C bond in the enone unit of hydroxylated chalcones—process arising from cyanobacterial adaptations

Author:
Żyszka-Haberecht, Beata; Poliwoda, Anna; Lipok, Jacek
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7097-7111
ISSN:
0175-7598
Subject:
Cyanobacteria; autotrophs; biohydrogenation; biosynthesis; biotransformation; chalcones; freshwater; hydrogenation; hydroxylation; mass spectrometry; nuclear magnetic resonance spectroscopy; polyphenols
Abstract:
... To verify the hypothesis that cyanobacteria naturally biosynthesising polyphenolic compounds possess an active enzymatic system that enables them to transform these substances, such an ability of the biocatalytic systems of whole cells of these biota was assessed for the first time. One halophilic strain and seven freshwater strains of cyanobacteria representing four of the five taxonomic orders o ...
DOI:
10.1007/s00253-018-9109-z

5. Biochemical characterization of an ulvan lyase from the marine flavobacterium Formosa agariphila KMM 3901T

Author:
Reisky, Lukas; Stanetty, Christian; Mihovilovic, Marko D.; Schweder, Thomas; Hehemann, Jan-Hendrik; Bornscheuer, Uwe T.
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6987-6996
ISSN:
0175-7598
Subject:
Flavobacterium; Ulva; algal blooms; aquatic bacteria; biomass; biorefining; calcium; carbon dioxide fixation; cations; cell walls; coasts; depolymerization; enzyme activity; gene overexpression; lyases; macroalgae; polysaccharides; sodium chloride
Abstract:
... Carbohydrates are the product of carbon dioxide fixation by algae in the ocean. Their polysaccharides are depolymerized by marine bacteria, with a vast array of carbohydrate-active enzymes. These enzymes are important tools to establish biotechnological processes based on algal biomass. Green tides, which cover coastal areas with huge amounts of algae from the genus Ulva, represent a globally risi ...
DOI:
10.1007/s00253-018-9142-y

6. Biotechnological applications of occlusion bodies of Baculoviruses

Author:
López, M. G.; Diez, M.; Alfonso, V.; Taboga, O.
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6765-6774
ISSN:
0175-7598
Subject:
Baculoviridae; antigens; biopesticides; biotechnology; genes; protein synthesis; proteins
Abstract:
... The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) ...
DOI:
10.1007/s00253-018-9130-2

7. Biotechnological production of cellulose by acetic acid bacteria: current state and perspectives

Author:
Gullo, Maria; La China, Salvatore; Falcone, Pasquale Massimiliano; Giudici, Paolo
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6885-6898
ISSN:
0175-7598
Subject:
acetic acid bacteria; biocompatibility; biodegradability; biofilm; biopolymers; cellulose; composite materials; cosmetics; crystal structure; engineering; nanomaterials; phenotype; polymerization; water holding capacity
Abstract:
... Bacterial cellulose is an attractive biopolymer for a number of applications including food, biomedical, cosmetics, and engineering fields. In addition to renewability and biodegradability, its unique structure and properties such as chemical purity, nanoscale fibrous 3D network, high water-holding capacity, high degree of polymerization, high crystallinity index, light transparency, biocompatibil ...
DOI:
10.1007/s00253-018-9164-5

8. Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2′-deoxyribosyltransferase from Chroococcidiopsis thermalis

Author:
Del Arco, Jon; Sánchez-Murcia, Pedro Alejandro; Mancheño, José Miguel; Gago, Federico; Fernández-Lucas, Jesús
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6947-6957
ISSN:
0175-7598
Subject:
Chroococcidiopsis thermalis; acid tolerance; cytosine; nucleosides; pH; purines; substrate specificity; temperature; therapeutics; thermal stability; transferases
Abstract:
... In our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50–100 °C) and a wide range of pH values (from 3 to 7). CtNDT ...
DOI:
10.1007/s00253-018-9134-y

9. Chlorination caused a shift in marine biofilm niches on microfiltration/ultrafiltration and reverse osmosis membranes and UV irradiation effectively inactivated a chlorine-resistant bacterium

Author:
Cho, Kyungjin; Jeong, Dawoon; Lee, Seockheon; Bae, Hyokwan
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7183-7194
ISSN:
0175-7598
Subject:
bacteria; biofilm; biofouling; chlorination; chlorine; disinfection; irradiation; microfiltration; niches; reverse osmosis; seawater; synergism; ultrafiltration; ultraviolet radiation
Abstract:
... The effect of chlorine disinfection on marine biofilm populations and communities formed on membrane surfaces was investigated under two feedwater conditions: raw seawater and deep bed filtration-treated seawater. As a result of chlorination, the structure of the biofilm community on the microfiltration/ultrafiltration and reverse osmosis membrane coupons shifted significantly at the genus level. ...
DOI:
10.1007/s00253-018-9111-5

10. Comparative analysis of fermentation and enzyme expression profiles among industrial Saccharomyces cerevisiae strains

Author:
Uebayashi, Kiyoka; Shimizu, Hiroshi; Matsuda, Fumio
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7071-7081
ISSN:
0175-7598
Subject:
Saccharomyces cerevisiae; breads; diploidy; domestication; environmental factors; enzymes; ethanol production; fermentation; genetic polymorphism; grape juice; proteome; sake; tricarboxylic acid cycle; wine yeasts
Abstract:
... Industrial diploid strains of Saccharomyces cerevisiae are selected from natural populations and then domesticated by optimizing the preferred properties for producing products such as bread, wine, and sake. In this study, for comparing the fermentation performance of various industrial yeasts, seven diploid strains of S. cerevisiae, namely, BY4947 (laboratory yeast derived from S288C), Kyokai7 an ...
DOI:
10.1007/s00253-018-9128-9

11. Comprehensive optimization of the metabolomic methodology for metabolite profiling of Corynebacterium glutamicum

Author:
Zhang, Qiongqiong; Zheng, Xiaomei; Wang, Yu; Yu, Jiandong; Zhang, Zhidan; Dele-Osibanjo, Taiwo; Zheng, Ping; Sun, Jibin; Jia, Shiru; Ma, Yanhe
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7113-7121
ISSN:
0175-7598
Subject:
Corynebacterium glutamicum; acetonitrile; biotin; ethanol; excretion; formic acid; glutamic acid; liquid chromatography; mass spectrometry; metabolism; metabolites; metabolomics; methanol; mutants
Abstract:
... Metabolomics has been a potential tool for strain improvement through analyzing metabolite changes in the context of different conditions. However, the availability of a universal metabolite profiling analysis is still a big challenge. In this study, we presented an optimized liquid chromatography-tandem mass spectrometry-based metabolomics methodology for Corynebacterium glutamicum, an important ...
DOI:
10.1007/s00253-018-9095-1

12. C-terminal Ser/Thr residues are vital for the regulatory role of Ste7 in the asexual cycle and virulence of Beauveria bassiana

Author:
Wang, Zhi-Kang; Cai, Qing; Tong, Sen-Miao; Ying, Sheng-Hua; Feng, Ming-Guang
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6973-6986
ISSN:
0175-7598
Subject:
Beauveria bassiana; adenosinetriphosphatase; antioxidants; blastospores; carbon; conidia; conidiation; entomopathogenic fungi; genes; germination; heat stress; homeostasis; hyphae; irradiation; mitogen-activated protein kinase; models; mutagenesis; nitrogen; oxidants; phenotype; pheromones; potassium; salts; stress response; stress tolerance; transcription (genetics); transcription factors; ultraviolet radiation; virulence; yeasts
Abstract:
... The mitogen-activated protein kinase (MAPK) kinase Ste7 has a conserved Ser/Thr loop (S/T-X₄₍₆₎-S/T) that can activate the MAPK Fus3 or Kss1 for the regulation of pheromone response and filamentous growth in model yeast. Here, we show that not only the loop but also four C-terminal Ser/Thr residues are essential for Ste7 to function in the Fus3 cascade of Beauveria bassiana, a filamentous fungal i ...
DOI:
10.1007/s00253-018-9148-5

13. Decay of sewage-associated bacterial communities in fresh and marine environmental waters and sediment

Author:
Ahmed, Warish; Staley, Christopher; Kaiser, Thomas; Sadowsky, Michael J.; Kozak, Sonya; Beale, David; Simpson, Stuart
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7159-7170
ISSN:
0175-7598
Subject:
DNA; bacterial communities; community structure; decision making; freshwater; guidelines; human health; microbiological quality; pathogens; risk; sediments; sewage; water quality; Queensland
Abstract:
... Understanding the microbial quality of recreational waters is critical to effectively managing human health risks. In recent years, the development of new molecular methods has provided scientists with alternatives to the use of culture-based fecal indicator methods for investigating sewage contamination in recreational waters. Before these methods can be formalized into guidelines, however, we mu ...
DOI:
10.1007/s00253-018-9112-4

14. Diverse roles of Hcp family proteins in the environmental fitness and pathogenicity of Aeromonas hydrophila Chinese epidemic strain NJ-35

Author:
Wang, Nannan; Liu, Jin; Pang, Maoda; Wu, Yafeng; Awan, Furqan; Liles, Mark R.; Lu, Chengping; Liu, Yongjie
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7083-7095
ISSN:
0175-7598
Subject:
Aeromonas hydrophila; Danio rerio; Tetrahymena; bacteria; bacterial adhesion; biofilm; eukaryotic cells; genes; hemolysins; mutants; predation; secretion; therapeutics; type VI secretion system; virulence
Abstract:
... The type VI secretion system (T6SS) has been considered as a crucial factor in bacterial competition and virulence. The hemolysin co-regulated protein (Hcp) is the hallmark of T6SS. The secretion of Hcp in Aeromonas hydrophila Chinese epidemic strain NJ-35 indicated a functional T6SS. In this study, three copies of the hcp gene were identified in the genome of strain NJ-35. We targeted these Hcp f ...
DOI:
10.1007/s00253-018-9116-0

15. Duplex real-time PCR assay for the simultaneous detection of Caliciopsis pinea and Fusarium circinatum in pine samples

Author:
Luchi, Nicola; Pepori, Alessia Lucia; Bartolini, Paola; Ioos, Renaud; Santini, Alberto
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7135-7146
ISSN:
0175-7598
Subject:
Caliciopsis pinea; DNA; European Union; Fusarium circinatum; Pinus; cankers (plants); conifers; diagnostic techniques; pathogens; quantitative polymerase chain reaction; quarantine; trees; Europe; United States
Abstract:
... Fusarium circinatum and Caliciopsis pinea are the causal agents of Pitch canker and Caliciopsis canker, respectively. These diseases affect pines and other conifers both in Europe and North America. The two pathogens cause similar bleeding cankers, especially at the early stage of colonization. Symptoms closely resembling those due to F. circinatum can be instead associated with C. pinea. Since F. ...
DOI:
10.1007/s00253-018-9184-1

16. EcXyl43 β-xylosidase: molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction

Author:
Ontañon, Ornella M.; Ghio, Silvina; Marrero Díaz de Villegas, Rubén; Piccinni, Florencia E.; Talia, Paola M.; Cerutti, María L.; Campos, Eleonora
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6959-6971
ISSN:
0175-7598
Subject:
Enterobacter; active sites; alpha-N-arabinofuranosidase; amino acid sequences; bioethanol; biomass; bioprocessing; biotransformation; endo-1,4-beta-xylanase; enzyme activity; ethanol; fermentation; functional properties; half life; high-value products; hydrolysis; lignocellulose; molecular models; mutagenesis; pH; polymerization; saccharification; synergism; thermal stability; wheat straw; xylan; xylan 1,4-beta-xylosidase; xylooligosaccharides; xylose
Abstract:
... Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires multiple enzymes acting concertedly. GH43 β-xylosidases are among the most interesting enzymes involved in hemicellulose deconstruction into xylose. In this work, the structural and ...
DOI:
10.1007/s00253-018-9138-7

17. Effective management of soft rot of ginger caused by Pythium spp. and Fusarium spp.: emerging role of nanotechnology

Author:
Rai, Mahendra; Ingle, Avinash P.; Paralikar, Priti; Anasane, Netravati; Gade, Rajendra; Ingle, Pramod
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6827-6839
ISSN:
0175-7598
Subject:
Fusarium; Pythium; Zingiber officinale; economic sustainability; fungi; fungicides; ginger; human health; medicinal properties; nanoparticles; rhizomes; risk; tropical plants
Abstract:
... Ginger (Zingiber officinale Rosc.) is a tropical plant cultivated all over the world due to its culinary and medicinal properties. It is one of the most important spices commonly used in food, which increases its commercial value. However, soft rot (rhizome rot) is a common disease of ginger caused by fungi such as Pythium and Fusarium spp. It is the most destructive disease of ginger, which can r ...
DOI:
10.1007/s00253-018-9145-8

18. Functions and impact of tal-like genes in animals with regard to applied aspects

Author:
Zhu, Min; Hu, Xiaolong; Cao, Guangli; Xue, Renyu; Gong, Chengliang
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6841-6845
ISSN:
0175-7598
Subject:
Drosophila; RNA; animals; eukaryotic cells; genes; metabolism; open reading frames; peptides
Abstract:
... A large number of DNAs in eukaryote genomes can code for atypical transcripts, and their functions are controversial. It has been reported that the transcripts contain many small open reading frames (sORFs), which were originally considered as non-translatable RNAs. However, increasing evidence has suggested that some of these sORFs can encode for small peptides and some are conserved across large ...
DOI:
10.1007/s00253-018-9159-2

19. Future direction in marine bacterial agarases for industrial applications

Author:
Jahromi, Saeid Tamadoni; Barzkar, Noora
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6847-6863
ISSN:
0175-7598
Subject:
Crustacea; agar; aquatic bacteria; coastal water; enzymes; industrial applications; macroalgae; marine ecosystems; marine environment; marine resources; sediments
Abstract:
... The marine ecosystem has been known to be a rich source of novel enzymes. Agarase is a key enzyme that can hydrolyze agar in the marine environment. Marine bacterial agarase has been isolated from various sources, such as sediments, coastal water, and deep sea and from the surface of crustaceans and seaweeds. This review presents an account of the agarase production of marine bacteria. General inf ...
DOI:
10.1007/s00253-018-9156-5

20. Hematite and multi-walled carbon nanotubes stimulate a faster syntrophic pathway during methanogenic beet sugar industrial wastewater degradation

Author:
Ambuchi, John Justo; Zhang, Zhaohan; Dong, Yue; Huang, Linlin; Feng, Yujie
Source:
Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7147-7158
ISSN:
0175-7598
Subject:
Archaea; Chloroflexi; Firmicutes; bacteria; beet sugar; carbon nanotubes; confocal laser scanning microscopy; ecosystems; electron transfer; electrons; hematite; industrial effluents; methane; methanogens; microbial communities; nanoparticles; organic matter; scanning electron microscopy
Abstract:
... The quest to understand and subsequently improve the role played by bacteria and archaea in the degradation of organic matter both in natural and engineered anaerobic ecosystems has intensified the utilization of nanoparticles. Microbial communities are known to syntrophically cooperate during the anaerobic conversion of substrates into methane gas via the direct exchange of electrons. In this stu ...
DOI:
10.1007/s00253-018-9100-8