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- Author:
- Martínez-Castro, Miriam; Barreiro, Carlos; Martín, Juan F.
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7029-7045
- ISSN:
- 0175-7598
- Subject:
- Streptomyces coelicolor; alkaline phosphatase; bacteria; bioinformatics; conserved sequences; gel electrophoresis; metabolism; models; nitrogen; nucleotides; phosphates; regulon; secondary metabolites; sequence analysis; tacrolimus
- Abstract:
- ... Inorganic and organic phosphate controls both primary and secondary metabolism in Streptomyces genus. Metabolism regulation by phosphate in Streptomyces species is mediated by the PhoR-PhoP two-component system. Response regulator PhoP binds to conserved sequences of 11 nucleotides called direct repeat units (DRus), whose organization and conservation determine the binding of PhoP to distinct prom ...
- DOI:
- 10.1007/s00253-018-9140-0
-
http://dx.doi.org/10.1007/s00253-018-9140-0
- Author:
- Takeya, Tomoyuki; Yurimoto, Hiroya; Sakai, Yasuyoshi
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7017-7027
- ISSN:
- 0175-7598
- Subject:
- screening; metabolism; detection limit; protein content; Komagataella pastoris; methanol; quantitative analysis; fluorescence; yeasts; flow cytometry; pectinesterase; geometry; genes; fluorescent proteins; biotransformation; enzyme activity; fluorescence microscopy; biosensors
- Abstract:
- ... We conducted single-cell analyses of the methylotrophic yeast Pichia pastoris to develop a biosensor for the detection of methanol produced by heterologous enzymes. In this biosensor, methanol and its subsequent metabolism induce expression of a gene encoding a fluorescent protein that was placed under the control of a methanol-inducible promoter. Using quantitative analyses of fluorescence micros ...
- DOI:
- 10.1007/s00253-018-9144-9
-
http://dx.doi.org/10.1007/s00253-018-9144-9
- Author:
- Mazzoli, Roberto; Giuffrida, Maria Gabriella; Pessione, Enrica
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6815-6825
- ISSN:
- 0175-7598
- Subject:
- bacteria; biodegradation; corrosion; cultural heritage; discoloration; enzymes; fabrics; monuments; paper; pigmentation; pollution; weathering
- Abstract:
- ... Ancient documents and milestones of human history such as manuscripts and textiles are fragile and during aging undergo chemical, physical, and biological deterioration. Among the different causes of damage, also human intervention plays a role since some restoration strategies proved to be transient and/or they generated further damage. Outdoor monuments undergo deterioration since they are expos ...
- DOI:
- 10.1007/s00253-018-9121-3
-
http://dx.doi.org/10.1007/s00253-018-9121-3
- Author:
- Żyszka-Haberecht, Beata; Poliwoda, Anna; Lipok, Jacek
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7097-7111
- ISSN:
- 0175-7598
- Subject:
- Cyanobacteria; autotrophs; biohydrogenation; biosynthesis; biotransformation; chalcones; freshwater; hydrogenation; hydroxylation; mass spectrometry; nuclear magnetic resonance spectroscopy; polyphenols
- Abstract:
- ... To verify the hypothesis that cyanobacteria naturally biosynthesising polyphenolic compounds possess an active enzymatic system that enables them to transform these substances, such an ability of the biocatalytic systems of whole cells of these biota was assessed for the first time. One halophilic strain and seven freshwater strains of cyanobacteria representing four of the five taxonomic orders o ...
- DOI:
- 10.1007/s00253-018-9109-z
- PubMed:
- 29869071
- PubMed Central:
- PMC6096841
-
http://dx.doi.org/10.1007/s00253-018-9109-z
- Author:
- Reisky, Lukas; Stanetty, Christian; Mihovilovic, Marko D.; Schweder, Thomas; Hehemann, Jan-Hendrik; Bornscheuer, Uwe T.
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6987-6996
- ISSN:
- 0175-7598
- Subject:
- Flavobacterium; Ulva; algal blooms; aquatic bacteria; biomass; biorefining; calcium; carbon dioxide fixation; cations; cell walls; coasts; depolymerization; enzyme activity; gene overexpression; lyases; macroalgae; polysaccharides; sodium chloride
- Abstract:
- ... Carbohydrates are the product of carbon dioxide fixation by algae in the ocean. Their polysaccharides are depolymerized by marine bacteria, with a vast array of carbohydrate-active enzymes. These enzymes are important tools to establish biotechnological processes based on algal biomass. Green tides, which cover coastal areas with huge amounts of algae from the genus Ulva, represent a globally risi ...
- DOI:
- 10.1007/s00253-018-9142-y
-
http://dx.doi.org/10.1007/s00253-018-9142-y
- Author:
- López, M. G.; Diez, M.; Alfonso, V.; Taboga, O.
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6765-6774
- ISSN:
- 0175-7598
- Subject:
- Baculoviridae; antigens; biopesticides; biotechnology; genes; protein synthesis; proteins
- Abstract:
- ... The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) ...
- DOI:
- 10.1007/s00253-018-9130-2
-
http://dx.doi.org/10.1007/s00253-018-9130-2
- Author:
- Gullo, Maria; La China, Salvatore; Falcone, Pasquale Massimiliano; Giudici, Paolo
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6885-6898
- ISSN:
- 0175-7598
- Subject:
- acetic acid bacteria; biocompatibility; biodegradability; biofilm; biopolymers; cellulose; composite materials; cosmetics; crystal structure; engineering; nanomaterials; phenotype; polymerization; water holding capacity
- Abstract:
- ... Bacterial cellulose is an attractive biopolymer for a number of applications including food, biomedical, cosmetics, and engineering fields. In addition to renewability and biodegradability, its unique structure and properties such as chemical purity, nanoscale fibrous 3D network, high water-holding capacity, high degree of polymerization, high crystallinity index, light transparency, biocompatibil ...
- DOI:
- 10.1007/s00253-018-9164-5
-
http://dx.doi.org/10.1007/s00253-018-9164-5
- Author:
- Del Arco, Jon; Sánchez-Murcia, Pedro Alejandro; Mancheño, José Miguel; Gago, Federico; Fernández-Lucas, Jesús
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6947-6957
- ISSN:
- 0175-7598
- Subject:
- Chroococcidiopsis thermalis; acid tolerance; cytosine; nucleosides; pH; purines; substrate specificity; temperature; therapeutics; thermal stability; transferases
- Abstract:
- ... In our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50–100 °C) and a wide range of pH values (from 3 to 7). CtNDT ...
- DOI:
- 10.1007/s00253-018-9134-y
-
http://dx.doi.org/10.1007/s00253-018-9134-y
- Author:
- Cho, Kyungjin; Jeong, Dawoon; Lee, Seockheon; Bae, Hyokwan
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7183-7194
- ISSN:
- 0175-7598
- Subject:
- bacteria; biofilm; biofouling; chlorination; chlorine; disinfection; irradiation; microfiltration; niches; reverse osmosis; seawater; synergism; ultrafiltration; ultraviolet radiation
- Abstract:
- ... The effect of chlorine disinfection on marine biofilm populations and communities formed on membrane surfaces was investigated under two feedwater conditions: raw seawater and deep bed filtration-treated seawater. As a result of chlorination, the structure of the biofilm community on the microfiltration/ultrafiltration and reverse osmosis membrane coupons shifted significantly at the genus level. ...
- DOI:
- 10.1007/s00253-018-9111-5
-
http://dx.doi.org/10.1007/s00253-018-9111-5
- Author:
- Uebayashi, Kiyoka; Shimizu, Hiroshi; Matsuda, Fumio
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7071-7081
- ISSN:
- 0175-7598
- Subject:
- Saccharomyces cerevisiae; breads; diploidy; domestication; environmental factors; enzymes; ethanol production; fermentation; genetic polymorphism; grape juice; proteome; sake; tricarboxylic acid cycle; wine yeasts
- Abstract:
- ... Industrial diploid strains of Saccharomyces cerevisiae are selected from natural populations and then domesticated by optimizing the preferred properties for producing products such as bread, wine, and sake. In this study, for comparing the fermentation performance of various industrial yeasts, seven diploid strains of S. cerevisiae, namely, BY4947 (laboratory yeast derived from S288C), Kyokai7 an ...
- DOI:
- 10.1007/s00253-018-9128-9
-
http://dx.doi.org/10.1007/s00253-018-9128-9
- Author:
- Zhang, Qiongqiong; Zheng, Xiaomei; Wang, Yu; Yu, Jiandong; Zhang, Zhidan; Dele-Osibanjo, Taiwo; Zheng, Ping; Sun, Jibin; Jia, Shiru; Ma, Yanhe
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7113-7121
- ISSN:
- 0175-7598
- Subject:
- Corynebacterium glutamicum; acetonitrile; biotin; ethanol; excretion; formic acid; glutamic acid; liquid chromatography; mass spectrometry; metabolism; metabolites; metabolomics; methanol; mutants
- Abstract:
- ... Metabolomics has been a potential tool for strain improvement through analyzing metabolite changes in the context of different conditions. However, the availability of a universal metabolite profiling analysis is still a big challenge. In this study, we presented an optimized liquid chromatography-tandem mass spectrometry-based metabolomics methodology for Corynebacterium glutamicum, an important ...
- DOI:
- 10.1007/s00253-018-9095-1
-
http://dx.doi.org/10.1007/s00253-018-9095-1
- Author:
- Wang, Zhi-Kang; Cai, Qing; Tong, Sen-Miao; Ying, Sheng-Hua; Feng, Ming-Guang
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6973-6986
- ISSN:
- 0175-7598
- Subject:
- Beauveria bassiana; adenosinetriphosphatase; antioxidants; blastospores; carbon; conidia; conidiation; entomopathogenic fungi; genes; germination; heat stress; homeostasis; hyphae; irradiation; mitogen-activated protein kinase; models; mutagenesis; nitrogen; oxidants; phenotype; pheromones; potassium; salts; stress response; stress tolerance; transcription (genetics); transcription factors; ultraviolet radiation; virulence; yeasts
- Abstract:
- ... The mitogen-activated protein kinase (MAPK) kinase Ste7 has a conserved Ser/Thr loop (S/T-X₄₍₆₎-S/T) that can activate the MAPK Fus3 or Kss1 for the regulation of pheromone response and filamentous growth in model yeast. Here, we show that not only the loop but also four C-terminal Ser/Thr residues are essential for Ste7 to function in the Fus3 cascade of Beauveria bassiana, a filamentous fungal i ...
- DOI:
- 10.1007/s00253-018-9148-5
-
http://dx.doi.org/10.1007/s00253-018-9148-5
- Author:
- Ahmed, Warish; Staley, Christopher; Kaiser, Thomas; Sadowsky, Michael J.; Kozak, Sonya; Beale, David; Simpson, Stuart
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7159-7170
- ISSN:
- 0175-7598
- Subject:
- DNA; bacterial communities; community structure; decision making; freshwater; guidelines; human health; microbiological quality; pathogens; risk; sediments; sewage; water quality; Queensland
- Abstract:
- ... Understanding the microbial quality of recreational waters is critical to effectively managing human health risks. In recent years, the development of new molecular methods has provided scientists with alternatives to the use of culture-based fecal indicator methods for investigating sewage contamination in recreational waters. Before these methods can be formalized into guidelines, however, we mu ...
- DOI:
- 10.1007/s00253-018-9112-4
-
http://dx.doi.org/10.1007/s00253-018-9112-4
- Author:
- Wang, Nannan; Liu, Jin; Pang, Maoda; Wu, Yafeng; Awan, Furqan; Liles, Mark R.; Lu, Chengping; Liu, Yongjie
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7083-7095
- ISSN:
- 0175-7598
- Subject:
- Aeromonas hydrophila; Danio rerio; Tetrahymena; bacteria; bacterial adhesion; biofilm; eukaryotic cells; genes; hemolysins; mutants; predation; secretion; therapeutics; type VI secretion system; virulence
- Abstract:
- ... The type VI secretion system (T6SS) has been considered as a crucial factor in bacterial competition and virulence. The hemolysin co-regulated protein (Hcp) is the hallmark of T6SS. The secretion of Hcp in Aeromonas hydrophila Chinese epidemic strain NJ-35 indicated a functional T6SS. In this study, three copies of the hcp gene were identified in the genome of strain NJ-35. We targeted these Hcp f ...
- DOI:
- 10.1007/s00253-018-9116-0
-
http://dx.doi.org/10.1007/s00253-018-9116-0
- Author:
- Luchi, Nicola; Pepori, Alessia Lucia; Bartolini, Paola; Ioos, Renaud; Santini, Alberto
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7135-7146
- ISSN:
- 0175-7598
- Subject:
- Caliciopsis pinea; DNA; European Union; Fusarium circinatum; Pinus; cankers (plants); conifers; diagnostic techniques; pathogens; quantitative polymerase chain reaction; quarantine; trees; Europe; United States
- Abstract:
- ... Fusarium circinatum and Caliciopsis pinea are the causal agents of Pitch canker and Caliciopsis canker, respectively. These diseases affect pines and other conifers both in Europe and North America. The two pathogens cause similar bleeding cankers, especially at the early stage of colonization. Symptoms closely resembling those due to F. circinatum can be instead associated with C. pinea. Since F. ...
- DOI:
- 10.1007/s00253-018-9184-1
-
http://dx.doi.org/10.1007/s00253-018-9184-1
- Author:
- Ontañon, Ornella M.; Ghio, Silvina; Marrero Díaz de Villegas, Rubén; Piccinni, Florencia E.; Talia, Paola M.; Cerutti, María L.; Campos, Eleonora
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6959-6971
- ISSN:
- 0175-7598
- Subject:
- Enterobacter; active sites; alpha-N-arabinofuranosidase; amino acid sequences; bioethanol; biomass; bioprocessing; biotransformation; endo-1,4-beta-xylanase; enzyme activity; ethanol; fermentation; functional properties; half life; high-value products; hydrolysis; lignocellulose; molecular models; mutagenesis; pH; polymerization; saccharification; synergism; thermal stability; wheat straw; xylan; xylan 1,4-beta-xylosidase; xylooligosaccharides; xylose
- Abstract:
- ... Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires multiple enzymes acting concertedly. GH43 β-xylosidases are among the most interesting enzymes involved in hemicellulose deconstruction into xylose. In this work, the structural and ...
- DOI:
- 10.1007/s00253-018-9138-7
-
http://dx.doi.org/10.1007/s00253-018-9138-7
- Author:
- Rai, Mahendra; Ingle, Avinash P.; Paralikar, Priti; Anasane, Netravati; Gade, Rajendra; Ingle, Pramod
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6827-6839
- ISSN:
- 0175-7598
- Subject:
- Fusarium; Pythium; Zingiber officinale; economic sustainability; fungi; fungicides; ginger; human health; medicinal properties; nanoparticles; rhizomes; risk; tropical plants
- Abstract:
- ... Ginger (Zingiber officinale Rosc.) is a tropical plant cultivated all over the world due to its culinary and medicinal properties. It is one of the most important spices commonly used in food, which increases its commercial value. However, soft rot (rhizome rot) is a common disease of ginger caused by fungi such as Pythium and Fusarium spp. It is the most destructive disease of ginger, which can r ...
- DOI:
- 10.1007/s00253-018-9145-8
-
http://dx.doi.org/10.1007/s00253-018-9145-8
- Author:
- Zhu, Min; Hu, Xiaolong; Cao, Guangli; Xue, Renyu; Gong, Chengliang
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6841-6845
- ISSN:
- 0175-7598
- Subject:
- Drosophila; RNA; animals; eukaryotic cells; genes; metabolism; open reading frames; peptides
- Abstract:
- ... A large number of DNAs in eukaryote genomes can code for atypical transcripts, and their functions are controversial. It has been reported that the transcripts contain many small open reading frames (sORFs), which were originally considered as non-translatable RNAs. However, increasing evidence has suggested that some of these sORFs can encode for small peptides and some are conserved across large ...
- DOI:
- 10.1007/s00253-018-9159-2
-
http://dx.doi.org/10.1007/s00253-018-9159-2
- Author:
- Jahromi, Saeid Tamadoni; Barzkar, Noora
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 6847-6863
- ISSN:
- 0175-7598
- Subject:
- Crustacea; agar; aquatic bacteria; coastal water; enzymes; industrial applications; macroalgae; marine ecosystems; marine environment; marine resources; sediments
- Abstract:
- ... The marine ecosystem has been known to be a rich source of novel enzymes. Agarase is a key enzyme that can hydrolyze agar in the marine environment. Marine bacterial agarase has been isolated from various sources, such as sediments, coastal water, and deep sea and from the surface of crustaceans and seaweeds. This review presents an account of the agarase production of marine bacteria. General inf ...
- DOI:
- 10.1007/s00253-018-9156-5
-
http://dx.doi.org/10.1007/s00253-018-9156-5
- Author:
- Ambuchi, John Justo; Zhang, Zhaohan; Dong, Yue; Huang, Linlin; Feng, Yujie
- Source:
- Applied microbiology and biotechnology 2018 v.102 no.16 pp. 7147-7158
- ISSN:
- 0175-7598
- Subject:
- Archaea; Chloroflexi; Firmicutes; bacteria; beet sugar; carbon nanotubes; confocal laser scanning microscopy; ecosystems; electron transfer; electrons; hematite; industrial effluents; methane; methanogens; microbial communities; nanoparticles; organic matter; scanning electron microscopy
- Abstract:
- ... The quest to understand and subsequently improve the role played by bacteria and archaea in the degradation of organic matter both in natural and engineered anaerobic ecosystems has intensified the utilization of nanoparticles. Microbial communities are known to syntrophically cooperate during the anaerobic conversion of substrates into methane gas via the direct exchange of electrons. In this stu ...
- DOI:
- 10.1007/s00253-018-9100-8
-
http://dx.doi.org/10.1007/s00253-018-9100-8