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- Katsimpouras, Constantinos; Dimarogona, Maria; Petropoulos, Pericles; Christakopoulos, Paul; Topakas, Evangelos
- Applied microbiology and biotechnology 2016 v.100 no.19 pp. 8385-8397
- konjac mannan, etc ; thermal stability; lignocellulose; Komagataella pastoris; sawdust; thermophilic fungi; genes; structure-activity relationships; reducing sugars; glucose; Myceliophthora thermophila; half life; yeasts; models; temperature; enzyme activity; glycosides; galactomannans; carob; enzymatic hydrolysis; pH; Show all 21 Subjects
- ... The endomannanase gene em26a from the thermophilic fungus Myceliophthora thermophila, belonging to the glycoside hydrolase family 26, was functionally expressed in the methylotrophic yeast Pichia pastoris. The putative endomannanase, dubbed MtMan26A, was purified to homogeneity (60 kDa) and subsequently characterized. The optimum pH and temperature for the enzymatic activity of MtMan26A were 6.0 a ...