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You searched for: Journal Applied microbiology and biotechnology Remove constraint Journal: Applied microbiology and biotechnology Publication Year 2007 Remove constraint Publication Year: 2007 Source 2007 v.75 no.1 Remove constraint Source: 2007 v.75 no.1
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21. Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge

Author:
Geets, Joke; de Cooman, Michaël; Wittebolle, Lieven; Heylen, Kim; Vanparys, Bram; De Vos, Paul; Verstraete, Willy; Boon, Nico
Source:
Applied microbiology and biotechnology 2007 v.75 no.1 pp. 211-221
ISSN:
0175-7598
Subject:
activated sludge; ammonia; community structure; denitrification; denitrifying bacteria; gene dosage; genes; microarray technology; nitrification; nitrites; nitrogen; oxidants; oxidation; phylogeny; quantitative polymerase chain reaction; wastewater treatment
Abstract:
... In order to improve wastewater treatment processes, a need exists for tools that rapidly give detailed insight into the community structure of activated sludge, supplementary to chemical and physical data. In this study, the advantages of microarrays and quantitative polymerase chin reaction (PCR) methods were combined into a real-time PCR assay that allows the simultaneous quantification of phylo ...
DOI:
10.1007/s00253-006-0805-8
PubMed:
17256118

22. Screening of β-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate

Author:
Fernandez, Rubén Cuervo; Ottoni, Cristiane Angélica; da Silva, Elda Sabino; Matsubara, Rosa Mitiko Saito; Carter, José Márcio; Magossi, Luis Roberto; Wada, Maria Alice Alves; de Andrade Rodrigues, Maria Filomena; Maresma, Beatriz Guilarte; Maiorano, Alfredo Eduardo
Source:
Applied microbiology and biotechnology 2007 v.75 no.1 pp. 87-93
ISSN:
0175-7598
Subject:
Aspergillus niger; Aspergillus oryzae; beta-fructofuranosidase; experimental design; fungi; pH; screening; temperature
Abstract:
... Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of β-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l⁻¹. In addition, the β-fructofuranosidases of those strains have a hi ...
DOI:
10.1007/s00253-006-0803-x

23. Secretory expression of a heterologous nattokinase in Lactococcus lactis

Author:
Liang, Xiaobo; Zhang, Lixin; Zhong, Jin; Huan, Liandong
Source:
Applied microbiology and biotechnology 2007 v.75 no.1 pp. 95-101
ISSN:
0175-7598
Subject:
Bacillus subtilis; Lactococcus lactis; Western blotting; atherosclerosis; signal peptide; thrombosis; u-plasminogen activator
Abstract:
... Nattokinase has been reported as an oral health product for the prevention of atherosclerosis. We developed a novel strategy to express a nattokinase from Bacillus subtilis in a live delivery vehicle, Lactococcus lactis. Promoter P ₙᵢₛZ and signal peptide SPUₛₚ were used for inducible and secretory expression of nattokinase in L. lactis. Western blotting analysis demonstrated that nattokinase was ...
DOI:
10.1007/s00253-006-0809-4
PubMed:
17225095

24. Susceptibility of staphylococcal biofilms to enzymatic treatments depends on their chemical composition

Author:
Chaignon, P.; Sadovskaya, I.; Ragunah, Ch.; Ramasubbu, N.; Kaplan, J. B.; Jabbouri, S.
Source:
Applied microbiology and biotechnology 2007 v.75 no.1 pp. 125-132
ISSN:
0175-7598
Subject:
Staphylococcus epidermidis; acids; antibiotics; bacteria; bacterial infections; biofilm; chemical composition; enzymatic treatment; immune system; orthopedics; pancreatin; peptidase K; prostheses; surgery; trypsin
Abstract:
... Bacterial infections are serious complications after orthopaedic implant surgery. Staphylococci, with Staphylococcus epidermidis as a leading species, are the prevalent and most important species involved in orthopaedic implant-related infections. The biofilm mode of growth of these bacteria on an implant surface protects the organisms from the host’s immune system and from antibiotic therapy. The ...
DOI:
10.1007/s00253-006-0790-y
PubMed:
17221196

25. The potential of cloud point system as a novel two-phase partitioning system for biotransformation

Author:
Wang, Zhilong
Source:
Applied microbiology and biotechnology 2007 v.75 no.1 pp. 1-10
ISSN:
0175-7598
Subject:
ambient temperature; biotransformation; industrial applications; ionic liquids; micelles; process control
Abstract:
... Although the extractive biotransformation in two-phase partitioning systems have been studied extensively, such as the water–organic solvent two-phase system, the aqueous two-phase system, the reverse micelle system, and the room temperature ionic liquid, etc., this has not yet resulted in a widespread industrial application. Based on the discussion of the main obstacles, an exploitation of a clou ...
DOI:
10.1007/s00253-007-0873-4
PubMed:
17318534