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- Page, W.J.; Bhanthumnavin, N.; Manchak, J.; Ruman, M.
- Applied microbiology and biotechnology 1997 v.48 no.1 pp. 88-93
- 3-hydroxybutyric acid; Azotobacter salinestris; Azotobacter vinelandii; acetates; aeration; composite polymers; enzymes; glucose; glyoxylate cycle; nitrogen; nitrogen fixation; polyhydroxyalkanoates; propionic acid; soil bacteria; succinic acid; valeric acid
- ... Azotobacter salinestris, a sodium-dependent, microaerophilic N2-fixing soil bacterium, formed polyhydroxyalkanoate copolymers comprised of beta-hydroxybutyric acid and 9-12 mol% beta-hydroxyvaleric acid (HV) during growth on sugars. Increased HV content was achieved by feeding valeric acid to the culture growing on glucose, but propionic acid could be directed to HV formation only when it served a ...
- Rando, D.; Giffhorn, G.W.K.F.
- Applied microbiology and biotechnology 1997 v.48 no.1 pp. 34-40
- Talaromyces pinophilus; carbon; cation exchange chromatography; fungi; gel chromatography; glucose; glucose oxidase; glycoproteins; half life; molecular weight; mycelium; pH; potassium phosphates; sodium citrate; stoichiometry; storage quality; sucrose; temperature
- ... A number of nutritional factors influencing growth and glucose oxidase (EC 18.104.22.168) production by a newly isolated strain of Penicillium pinophilum were investigated. The most important factors for glucose oxidase production were the use of sucrose as the carbon source, and growth of the fungus at non-optimal pH 6.5. The enzyme was purified to apparent homogeneity with a yield of 74% including an ...