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- Crous, J.M.; Pretorius, I.S.; Van Zyl, W.H.
- Applied microbiology and biotechnology 1996 v.46 no.3 pp. 256-260
- Aspergillus niger; DNA fragmentation; Saccharomyces cerevisiae; alpha-N-arabinofuranosidase; complementary DNA; gene expression; genes; messenger RNA; nucleotide sequences; oats; phosphoglycerate kinase; plasmids; polymerase chain reaction; xylan; yeasts
- ... First-strand cDNA was prepared from mRNA of Aspergillus niger MRC11624 induced on oat spelts xylan. Using the cDNA as a template, the alpha-L-arabinofuranosidase gene (abfB) was amplified with the polymerase chain reaction technique. The abfB DNA fragment was inserted between the yeast phosphoglycerate kinase I gene promoter (PGK1p) and terminator (PGK1T) sequences on a multicopy episomal plasmid. ...