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1. Quorum sensing as a potential target for increased production of rhamnolipid biosurfactant in Burkholderia thailandensis E264

Author:
Marchant, Roger,   et al.   ; Victor, Irorere U.; Kwiencien, Michal; Tripathi, Lakshmi; Cobice, Diego; McClean, Stephen; Banat, Ibrahim M.;     Show all 7 Authors
Source:
Applied microbiology and biotechnology 2019 v.103 no.16 pp. 6505-6517
ISSN:
0175-7598
Subject:
Burkholderia thailandensis; Pseudomonas aeruginosa; biosurfactants; biosynthesis; energy; fermentation; glycerol; mutants; mutation; polyhydroxyalkanoates; quorum sensing; rhamnolipids
Abstract:
... Burkholderia thailandensis E264 is a potential non-pathogenic substitute for producing rhamnolipid biosurfactant, replacing the pathogenic Pseudomonas aeruginosa. However, it has low rhamnolipid production and longer fermentation time. We have earlier suggested that media supplementation with exogenous quorum sensing (QS) molecules could lead to early onset of biosynthesis and increased rhamnolipi ...
DOI:
10.1007/s00253-019-09942-5
PubMed:
31222386
PubMed Central:
PMC6667413

2. Natural quorum sensing inhibitors effectively downregulate gene expression of Pseudomonas aeruginosa virulence factors

Author:
Marchant, Roger,   et al.   ; Ahmed, Syed A. K. S.; Rudden, Michelle; Smyth, Thomas J.; Dooley, James S. G.; Banat, Ibrahim M.;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2019 v.103 no.8 pp. 3521-3535
ISSN:
0175-7598
Subject:
Pseudomonas aeruginosa; adjuvants; antibacterial properties; antibiotics; bacteria; biochemical pathways; biofilm; developmental stages; drugs; elastase; gene expression; gene expression regulation; genes; pathogenesis; phenotype; pyocyanin; quorum sensing; rhamnolipids; salicylic acid; therapeutics; transcription (genetics); virulence
Abstract:
... At present, anti-virulence drugs are being considered as potential therapeutic alternatives and/or adjuvants to currently failing antibiotics. These drugs do not kill bacteria but inhibit virulence factors essential for establishing infection and pathogenesis through targeting non-essential metabolic pathways reducing the selective pressure to develop resistance. We investigated the effect of natu ...
DOI:
10.1007/s00253-019-09618-0
PubMed:
30852658
PubMed Central:
PMC6449319

3. Microbial reductive dehalogenation of trihalomethanes by a Dehalobacter-containing co-culture

Author:
Rogers, Matthew J.,   et al.   ; Zhao, Siyan; He, Jianzhong;     Show all 3 Authors
Source:
Applied microbiology and biotechnology 2017 v.101 no.13 pp. 5481-5492
ISSN:
0175-7598
Subject:
Dehalobacter; chloroform; coculture; dehalogenation; disinfection; genes; groundwater; half life; methylene chloride; microbial communities; prediction; soil; substrate specificity; tetrachloroethylene
Abstract:
... Trihalomethanes such as chloroform and bromoform, although well-known as a prominent class of disinfection by-products, are ubiquitously distributed in the environment due to widespread industrial usage in the past decades. Chloroform and bromoform are particularly concerning, of high concentrations detected and with long half-lives up to several hundred days in soils and groundwater. In this stud ...
DOI:
10.1007/s00253-017-8236-2

4. Fatty acid synthesis pathway provides lipid precursors for rhamnolipid biosynthesis in Burkholderia thailandensis E264

Author:
Marchant, Roger,   et al.   ; Irorere, Victor U.; Smyth, Thomas J.; Cobice, Diego; McClean, Stephen; Banat, Ibrahim M.;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2018 v.102 no.14 pp. 6163-6174
ISSN:
0175-7598
Subject:
Burkholderia thailandensis; Galleria mellonella; Pseudomonas aeruginosa; biosynthesis; carbon; deuterium; fermentation; glycerol; isotope labeling; lethal concentration 50; models; moieties; pathogenicity; rhamnolipids; stearic acid
Abstract:
... Rhamnolipid production was monitored for a period of 216 h using different substrates in Pseudomonas aeruginosa PAO1 and Burkholderia thailandensis E264 which showed comparable crude yields attained by both after 216 h. The crude yield for P. aeruginosa, however, was significantly higher at the early stages of fermentation (72 or 144 h). Additionally, P. aeruginosa produced rhamnolipid with odd an ...
DOI:
10.1007/s00253-018-9059-5
PubMed:
29752487
PubMed Central:
PMC6013509

5. Enhanced rhamnolipid production in Burkholderia thailandensis transposon knockout strains deficient in polyhydroxyalkanoate (PHA) synthesis

Author:
Marchant, Roger,   et al.   ; Funston, Scott J.; Tsaousi, Konstantina; Smyth, Thomas J.; Twigg, Matthew S.; Banat, Ibrahim M.;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2017 v.101 no.23-24 pp. 8443-8454
ISSN:
0175-7598
Subject:
Burkholderia thailandensis; Pseudomonas aeruginosa; animal pathogens; genes; mutants; mutation; polyhydroxyalkanoates; rhamnolipids; transposons
Abstract:
... Microbially produced rhamnolipids have significant commercial potential; however, the main bacterial producer, Pseudomonas aeruginosa, is an opportunistic human pathogen, which limits biotechnological exploitation. The non-pathogenic species Burkholderia thailandensis produces rhamnolipids; however, yield is relatively low. The aim of this study was to determine whether rhamnolipid production coul ...
DOI:
10.1007/s00253-017-8540-x
PubMed:
29043376
PubMed Central:
PMC5694511

6. Microbial rhamnolipid production: a critical re-evaluation of published data and suggested future publication criteria

Author:
Marchant, Roger,   et al.   ; Irorere, Victor U.; Tripathi, Lakshmi; McClean, Stephen; Banat, Ibrahim M.;     Show all 5 Authors
Source:
Applied microbiology and biotechnology 2017 v.101 no.10 pp. 3941-3951
ISSN:
0175-7598
Subject:
Burkholderia pseudomallei; Burkholderia thailandensis; Pseudomonas aeruginosa; bioinformatics; cost effectiveness; genes; gravimetry; high performance liquid chromatography; pathogenicity; rhamnolipids; ultra-performance liquid chromatography
Abstract:
... High production cost and potential pathogenicity of Pseudomonas aeruginosa, commonly used for rhamnolipid synthesis, have led to extensive research for safer producing strains and cost-effective production methods. This has resulted in numerous research publications claiming new non-pathogenic producing strains and novel production techniques many of which are unfortunately without proper characte ...
DOI:
10.1007/s00253-017-8262-0
PubMed:
28386631
PubMed Central:
PMC5403872

7. Silica sol-gel encapsulation of cyanobacteria: lessons for academic and applied research

Author:
Ely, Roger L.,   et al.   ; Dickson, David J.;     Show all 2 Author
Source:
Applied microbiology and biotechnology 2013 v.97 no.5 pp. 1809-1819
ISSN:
0175-7598
Subject:
Synechocystis; biogeochemical cycles; biomass; biotechnology; carbon; ecosystems; encapsulation; food additives; glycerol; nitrogen fixation; photosynthesis; pigments; proteins; silica gel
Abstract:
... Cyanobacteria inhabit nearly every ecosystem on earth, play a vital role in nutrient cycling, and are useful as model organisms for fundamental research in photosynthesis and carbon and nitrogen fixation. In addition, they are important for several established biotechnologies for producing food additives, nutritional and pharmaceutical compounds, and pigments, as well as emerging biotechnologies f ...
DOI:
10.1007/s00253-012-4686-8
PubMed:
23321911

8. Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer

Author:
Marchant, Roger,   et al.   ; Funston, Scott J.; Tsaousi, Konstantina; Rudden, Michelle; Smyth, Thomas J.; Stevenson, Paul S.; Banat, Ibrahim M.;     Show all 7 Authors
Source:
Applied microbiology and biotechnology 2016 v.100 no.18 pp. 7945-7956
ISSN:
0175-7598
Subject:
Burkholderia thailandensis; Gram-negative bacteria; biomass production; bioreactors; biosynthesis; fermentation; genes; quantitative polymerase chain reaction; rhamnolipids; soil; temperature; Thailand
Abstract:
... Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, in ...
DOI:
10.1007/s00253-016-7564-y
PubMed:
27147528
PubMed Central:
PMC4989024

9. Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative determination of rhamnolipid congeners

Author:
Marchant, Roger,   et al.   ; Rudden, Michelle; Tsauosi, Konstantina; Banat, Ibrahim M.; Smyth, Thomas J.;     Show all 5 Authors
Source:
Applied microbiology and biotechnology 2015 v.99 no.21 pp. 9177-9187
ISSN:
0175-7598
Subject:
Pseudomonas aeruginosa; carbon; fatty acids; guidelines; high performance liquid chromatography; isomers; monitoring; quantitative analysis; rhamnose; tandem mass spectrometry; ultra-performance liquid chromatography; yields
Abstract:
... Rhamnolipids (RLs) are synthesised as a complex mixture of congeners comprising either one or two molecules of rhamnose glycosidically linked to a dimer of 3-hydroxy fatty acids varying in chain length and degree of saturation. Currently, HPLC-MS/MS is the most precise and accurate method for RL determination, while accurate quantification is limited. In this study, a rapid ultra pressure liquid c ...
DOI:
10.1007/s00253-015-6837-1

10. Evaluation of encapsulation stress and the effect of additives on viability and photosynthetic activity of Synechocystis sp. PCC 6803 encapsulated in silica gel

Author:
Ely, Roger L.,   et al.   ; Dickson, David J.;     Show all 2 Author
Source:
Applied microbiology and biotechnology 2011 v.91 no.6 pp. 1633-1646
ISSN:
0175-7598
Subject:
Synechocystis; additive effect; additives; betaine; encapsulation; energy; ethanol; gels; glycerol; photosystem II; polyethylene glycol; salt stress; salts; silica gel; viability
Abstract:
... Stresses imposed on the cyanobacterium Synechocystis sp. PCC 6803 by various compounds present during silica sol–gel encapsulation, including salt, ethanol (EtOH), polyethylene glycol (PEG), glycerol, and glycine betaine, were investigated. Viability of encapsulated cells and photosynthetic activity of cells stressed by immediate (2 min) and 24-h exposure to the five stress-inducing compounds were ...
DOI:
10.1007/s00253-011-3517-7
PubMed:
21830041

11. The role of lipids in the biogenesis of integral membrane proteins

Author:
Schneiter, Roger,   et al.   ; Toulmay, Alexandre;     Show all 2 Author
Source:
Applied microbiology and biotechnology 2007 v.73 no.6 pp. 1224-1232
ISSN:
0175-7598
Subject:
biogenesis; endoplasmic reticulum; enzyme activity; eukaryotic cells; lipid bilayers; lipid composition; lipids; membrane proteins; neonates; plasma membrane; prokaryotic cells; topology
Abstract:
... Most integral membrane proteins are cotranslationally inserted into the lipid bilayer. In prokaryotes, membrane insertion of the nascent chain takes place at the plasma membrane, whereas in eukaryotes insertion takes place into the endoplasmatic reticulum. In both kingdoms of life, however, the same membrane that acquaints the newly born membrane protein also synthesizes the bilayer lipids and thu ...
DOI:
10.1007/s00253-006-0707-9
PubMed:
17111137

12. International Space Station environmental microbiome — microbial inventories of ISS filter debris

Author:
Rogers, Scott O.,   et al.   ; Venkateswaran, Kasthuri; Vaishampayan, Parag; Cisneros, Jessica; Pierson, Duane L.; Perry, Jay;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2014 v.98 no.14 pp. 6453-6466
ISSN:
0175-7598
Subject:
Corynebacterium; Propionibacterium; Staphylococcus; adenosine triphosphate; fungi; high-throughput nucleotide sequencing; inventories; microbiome; phylogeny; plate count; propidium; quantitative polymerase chain reaction; viability
Abstract:
... Despite an expanding array of molecular approaches for detecting microorganisms in a given sample, rapid and robust means of assessing the differential viability of the microbial cells, as a function of phylogenetic lineage, remain elusive. A propidium monoazide (PMA) treatment coupled with downstream quantitative polymerase chain reaction (qPCR) and pyrosequencing analyses was carried out to bett ...
DOI:
10.1007/s00253-014-5650-6
PubMed:
24695826

13. Transcriptomic responses of Synechocystis sp. PCC 6803 encapsulated in silica gel

Author:
Ely, Roger L.,   et al.   ; Dickson, David J.; Luterra, Markael D.;     Show all 3 Authors
Source:
Applied microbiology and biotechnology 2012 v.96 no.1 pp. 183-196
ISSN:
0175-7598
Subject:
RNA; Synechocystis; alcohols; biofuels; encapsulation; gels; gene expression; genes; microarray technology; microorganisms; proteins; salt stress; silica gel
Abstract:
... Global gene expression of Synechocystis sp. PCC 6803 encapsulated in silica gel was examined by microarray analysis. Cultures were encapsulated in gels derived from aqueous precursors or from alkoxide precursors and incubated under constant light for 24 h prior to RNA extraction. Cultures suspended in liquid media were exposed to 500 mM salt stress and incubated under identical conditions for comp ...
DOI:
10.1007/s00253-012-4307-6
PubMed:
22846903

14. Genome-wide transcriptomic analysis of a flocculent strain of Zymomonas mobilis

Author:
Rogers, Peter L.,   et al.   ; Jeon, Young Jae; Xun, Zhao; Su, Ping;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 2012 v.93 no.6 pp. 2513-2518
ISSN:
0175-7598
Subject:
sequence analysis; transcriptomics; protein synthesis; cell movement; ethanol; gene overexpression; recycling; flagellum; cellulose; amino acids; genes; nucleotide sequences; biomass; gene expression regulation; batch fermentation; point mutation; Zymomonas mobilis
Abstract:
... ZM401, a flocculent mutant strain of Zymomonas mobilis ZM4 was studied using genome-wide transcriptomic analysis for evidence related to phenotypic changes associated with its cell–cell attachment behaviour. Batch fermentation studies with ZM401 and its parent strain ZM4 demonstrated that similar ethanol yields and productivities could be achieved with both strains indicating the potential of the ...
DOI:
10.1007/s00253-012-3948-9
PubMed:
22350321

15. Targeting surface-layer proteins with single-domain antibodies: a potential therapeutic approach against Clostridium difficile-associated disease

Author:
MacKenzie, Roger,   et al.   ; Kandalaft, Hiba; Hussack, Greg; Aubry, Annie; van Faassen, Henk; Guan, Yonghong; Arbabi-Ghahroudi, Mehdi; Logan, Susan M.; Tanha, Jamshid;     Show all 9 Authors
Source:
Applied microbiology and biotechnology 2015 v.99 no.20 pp. 8549-8562
ISSN:
0175-7598
Subject:
Clostridium difficile; antibiotics; antibodies; bacteriophages; death; enzyme-linked immunosorbent assay; epitopes; gastrointestinal system; hamsters; immunization; melting; neutralization; pepsin; proteins; relapse; ribotypes; temperature; North America
Abstract:
... Clostridium difficile is a leading cause of death from gastrointestinal infections in North America. Antibiotic therapy is effective, but the high incidence of relapse and the rise in hypervirulent strains warrant the search for novel treatments. Surface layer proteins (SLPs) cover the entire C. difficile bacterial surface, are composed of high-molecular-weight (HMW) and low-molecular-weight (LMW) ...
DOI:
10.1007/s00253-015-6594-1
PubMed:
25936376
PubMed Central:
PMC4768215

16. Rhamnolipids are conserved biosurfactants molecules: implications for their biotechnological potential

Author:
Marchant, Roger,   et al.   ; Perfumo, Amedea; Rudden, Michelle; Smyth, Thomas J. P.; Stevenson, Paul S.; Parry, Neil J.; Banat, Ibrahim M.;     Show all 7 Authors
Source:
Applied microbiology and biotechnology 2013 v.97 no.16 pp. 7297-7306
ISSN:
0175-7598
Subject:
Pseudomonas aeruginosa; amino acid sequences; culture media; gene expression; gene expression regulation; genes; strain differences
Abstract:
... A range of isolates of Pseudomonas aeruginosa from widely different environmental sources were examined for their ability to synthesise rhamnolipid biosurfactants. No significant differences in the quantity or composition of the rhamnolipid congeners could be produced by manipulating the growth conditions. Sequences for the rhamnolipid genes indicated low levels of strain variation, and the majori ...
DOI:
10.1007/s00253-013-4876-z
PubMed:
23563913

17. The medicinal Agaricus mushroom cultivated in Brazil: biology, cultivation and non-medicinal valorisation

Author:
Regnault-Roger, Catherine,   et al.   ; Largeteau, Michèle L.; Llarena-Hernández, Régulo Carlos; Savoie, Jean-Michel;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 2011 v.92 no.5 pp. 897-907
ISSN:
0175-7598
Subject:
Agaricus bisporus; cultivars; enzymes; food additives; genetic variation; medicinal properties; models; mushrooms; nutritive value; odors; plant pathogens; taste; Brazil
Abstract:
... Sun mushroom is a cultivated mushroom extensively studied for its medicinal properties for several years and literature abounds on the topic. Besides, agronomical aspects were investigated in Brazil, the country the mushroom comes from, and some studies focus on the biology of the fungus. This review aimed to present an overview of the non-medicinal knowledge on the mushroom. Areas of commercial p ...
DOI:
10.1007/s00253-011-3630-7
PubMed:
22005742

18. DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4

Author:
Rogers, Peter L.,   et al.   ; Kerr, Aidan L.; Jeon, Young Jae; Svenson, Charles J.; Neilan, Brett A.;     Show all 5 Authors
Source:
Applied microbiology and biotechnology 2011 v.89 no.3 pp. 761-769
ISSN:
0175-7598
Subject:
Zymomonas mobilis; gene targeting; genes; glucose; knockout mutants; plasmids; specific growth rate
Abstract:
... To better understand the DNA restriction-modification (R-M) systems for more amenable strain development of the alternative industrial ethanologen, Zymomonas mobilis, three gene knockout mutants were constructed. The gene knockout mutants were tested for their DNA restriction activities by the determination of transformation efficiency using methylated and unmethylated foreign plasmid DNAs. Inacti ...
DOI:
10.1007/s00253-010-2936-1
PubMed:
20957358

19. Expression of phenol oxidase and heat-shock genes during the development of Agaricus bisporus fruiting bodies, healthy and infected by Lecanicillium fungicola

Author:
Regnault-Roger, Catherine,   et al.   ; Largeteau, Michèle L.; Latapy, Camille; Minvielle, Nathalie; Savoie, Jean-Michel;     Show all 5 Authors
Source:
Applied microbiology and biotechnology 2010 v.85 no.5 pp. 1499-1507
ISSN:
0175-7598
Subject:
heat shock proteins; monophenol monooxygenase; cultivars; mushrooms; Agaricus bisporus; bubbles; host-pathogen relationships; industry; morphogenesis; mycelium; heat stress; genes; Lecanicillium fungicola; laccase; fruiting bodies; pathogens; crops
Abstract:
... The fungal pathogen Lecanicillium fungicola (formerly Verticillium fungicola) is responsible for severe losses worldwide in the mushroom (Agaricus bisporus) industry. Infected crops are characterised by masses of undifferentiated tissue (bubbles) growing in place of sporophores. The expression of three laccase genes (lcc1, lcc2 and lcc3), two tyrosinase genes (AbPPO1 and AbPPO2) and the hspA gene ...
DOI:
10.1007/s00253-009-2186-2
PubMed:
19711071

20. Specific single-cell isolation and genomic amplification of uncultured microorganisms

Author:
Lasken, Roger S.,   et al.   ; Kvist, Thomas; Ahring, Birgitte K.; Westermann, Peter;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 2007 v.74 no.4 pp. 926-935
ISSN:
0175-7598
Subject:
Archaea; DNA-directed DNA polymerase; bacterial artificial chromosomes; fluorescence; fluorescence in situ hybridization; genes; genomics; microorganisms; nucleotide sequences; restriction fragment length polymorphism; ribosomal RNA; sequence homology; soil
Abstract:
... We in this study describe a new method for genomic studies of individual uncultured prokaryotic organisms, which was used for the isolation and partial genome sequencing of a soil archaeon. The diversity of Archaea in a soil sample was mapped by generating a clone library using group-specific primers in combination with a terminal restriction fragment length polymorphism profile. Intact cells were ...
DOI:
10.1007/s00253-006-0725-7
PubMed:
17109170

21. Rhamnolipid production by a novel thermophilic hydrocarbon-degrading Pseudomonas aeruginosa AP02-1

Author:
Marchant, Roger,   et al.   ; Perfumo, Amedea; Banat, Ibrahim M.; Canganella, Francesco;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 2006 v.72 no.1 pp. 132-138
ISSN:
0175-7598
Subject:
Pseudomonas aeruginosa; alkanes; biosurfactants; carbon; diesel fuel; emulsions; glycerol; hot springs; hydrophobicity; mass spectrometry; microbial growth; polycyclic aromatic hydrocarbons; salts; surface tension; temperature
Abstract:
... Thermophilic bacterial cultures were isolated from a hot spring environment on hydrocarbon containing mineral salts media. One strain identified as Pseudomonas aeruginosa AP02-1 was tested for the ability to utilize a range of hydrocarbons both n-alkanes and polycyclic aromatic hydrocarbons as sole carbon source. Strain AP02-1 had an optimum growth temperature of 45°C and degraded 99% of crude oil ...
DOI:
10.1007/s00253-005-0234-0
PubMed:
16344932

22. Physico-chemical properties of polyhydroxyalkanoate produced by mixed-culture nitrogen-fixing bacteria

Author:
Newman, Roger H.,   et al.   ; Patel, Meeta; Gapes, Daniel J.; Dare, Peter H.;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 2009 v.82 no.3 pp. 545-555
ISSN:
0175-7598
Subject:
biodegradability; biomass; biopolyesters; carbon; composite polymers; crystal structure; differential scanning calorimetry; economic sustainability; endothermy; enthalpy; gel chromatography; melting; mixed culture; molecular weight; nitrogen-fixing bacteria; nuclear magnetic resonance spectroscopy; physical properties; poly-3-hydroxybutyrate
Abstract:
... Ultra-high molecular weight polyhydroxyalkanoates (PHAs) with low polydispersity index (PDI = 1.3) were produced in a novel, pilot scale application of mixed cultures of nitrogen-fixing bacteria. The number average molecular weight (M ₙ) of the poly(3-hydroxybutyrate) (P(3HB)) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) was determined to be 2.4 × 10⁶ and 2.5 × 10⁶ g mol⁻¹, res ...
DOI:
10.1007/s00253-008-1836-0

23. Enzymatic (R)-phenylacetylcarbinol production in benzaldehyde emulsions

Author:
Rogers,,   et al.   ; Rosche,; Leksawasdi,; Sandford,; Breuer,; Hauer,;     Show all 6 Authors
Source:
Applied microbiology and biotechnology 2002 v.60 no.1-2 pp. 94-100
ISSN:
0175-7598
Subject:
Rhizopus; acetaldehyde; acetoin; benzaldehyde; biotransformation; catalysts; emulsions; enzyme stability; enzyme substrates; ephedrine; ethanol; fungi; pH; potassium chloride; pseudoephedrine; pyruvate decarboxylase; pyruvic acid; solubility; temperature
Abstract:
... (R)-Phenylacetylcarbinol [(R)-PAC)] is the chiral precursor for the production of the pharmaceuticals ephedrine and pseudoephedrine. Reaction conditions were improved to achieve increased (R)-PAC levels in a simple batch biotransformation of benzaldehyde emulsions and pyruvate, using partially purified pyruvate decarboxylase (PDC) from the filamentous fungus Rhizopus javanicus NRRL 13161 as the ca ...
DOI:
10.1007/s00253-002-1084-7

24. Microbial biosurfactants production, applications and future potential

Author:
Marchant, Roger,   et al.   ; Banat, Ibrahim M.; Franzetti, Andrea; Gandolfi, Isabella; Bestetti, Giuseppina; Martinotti, Maria G.; Fracchia, Letizia; Smyth, Thomas J.;     Show all 8 Authors
Source:
Applied microbiology and biotechnology 2010 v.87 no.2 pp. 427-444
ISSN:
0175-7598
Subject:
air; biosurfactants; emulsifiers; emulsions; industrial applications; mechanism of action; microorganisms; molecular weight; oils; production costs; surface tension
Abstract:
... Microorganisms synthesise a wide range of surface-active compounds (SAC), generally called biosurfactants. These compounds are mainly classified according to their molecular weight, physico-chemical properties and mode of action. The low-molecular-weight SACs or biosurfactants reduce the surface tension at the air/water interfaces and the interfacial tension at oil/water interfaces, whereas the hi ...
DOI:
10.1007/s00253-010-2589-0
PubMed:
20424836

25. Directed microbial biosynthesis of deuterated biosurfactants and potential future application to other bioactive molecules

Author:
Marchant, Roger,   et al.   ; Smyth, Thomas J.; Perfumo, Amedea; Banat, Ibrahim M.; Chen, Minglei; Thomas, Robert K.; Penfold, Jeffrey; Stevenson, Paul S.; Parry, Neil J.;     Show all 9 Authors
Source:
Applied microbiology and biotechnology 2010 v.87 no.4 pp. 1347-1354
ISSN:
0175-7598
Subject:
Pseudomonas aeruginosa; Starmerella bombicola; biosurfactants; biosynthesis; carbon; deuterium; deuterium oxide; genes; isomers; rhamnolipids; sophorolipids; sugars; transferases; yeasts
Abstract:
... Deuterated rhamnolipids were produced using strain AD7 of Pseudomonas aeruginosa, which was progressively adapted to increasing levels of deuterium in D₂O and carbon substrates. Fourteen different deuterated rhamnolipid structures, including structural isomers, were produced which is similar to normal protonated structures. There were two main products monorhamnolipid Rha-C₁₀-C₁₀ and dirhamnolipid ...
DOI:
10.1007/s00253-010-2592-5
PubMed:
20405122

26. Thermophilic bacteria in cool temperate soils: are they metabolically active or continually added by global atmospheric transport?

Author:
Marchant, Roger,   et al.   ; Franzetti, Andrea; Pavlostathis, Spyros G.; Tas, Didem Okutman; Erdbrűgger, Isabel; Űnyayar, Ali; Mazmanci, Mehmet A.; Banat, Ibrahim M.;     Show all 8 Authors
Source:
Applied microbiology and biotechnology 2008 v.78 no.5 pp. 841-852
ISSN:
0175-7598
Subject:
Geobacillus; aerobic conditions; air; ambient temperature; biogeography; denitrification; mortality; population; population growth; rain; temperate soils; thermophilic bacteria; viability
Abstract:
... Thermophilic soil geobacilli isolated from cool temperate geographical zone environments have been shown to be metabolically inactive under aerobic conditions at ambient temperatures (−5 to 25°C). It is now confirmed that a similar situation exists for their anaerobic denitrification activity. It is necessary therefore to determine the mechanisms that sustain the observed significant viable popula ...
DOI:
10.1007/s00253-008-1372-y
PubMed:
18256821

27. Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans

Author:
Koukiekolo, Roger,   et al.   ; Arai, Takamitsu; Kosugi, Akihiko; Chan, Helen; Yukawa, Hideaki; Inui, Masayuki; Doi, Roy H.;     Show all 7 Authors
Source:
Applied microbiology and biotechnology 2006 v.71 no.5 pp. 654-660
ISSN:
0175-7598
Subject:
Clostridium cellulovorans; carbohydrate binding; carboxymethylcellulose; catalytic activity; cleavage (chemistry); endo-1,4-beta-glucanase; enzyme activity; genes; molecular cloning; sequence analysis; thin layer chromatography
Abstract:
... The cellulosomal family 9 cellulase genes engH, engK, engL, engM, and engY of Clostridium cellulovorans have been cloned and sequenced. We compared the enzyme activity of family 9 cellulosomal cellulases from C. cellulovorans and their derivatives. EngH has the highest activity toward soluble cellulose derivatives such as carboxymethylcellulose (CMC) as well as insoluble cellulose such as acid-swo ...
DOI:
10.1007/s00253-005-0249-6

28. Enzymatic (R)-phenylacetylcarbinol production in a benzaldehyde emulsion system with Candida utilis cells

Author:
Rogers, Peter L.,   et al.   ; Satianegara, Gernalia; Breuer, Michael; Hauer, Bernhard; Rosche, Bettina;     Show all 5 Authors
Source:
Applied microbiology and biotechnology 2006 v.70 no.2 pp. 170-175
ISSN:
0175-7598
Subject:
Candida utilis; benzaldehyde; benzyl alcohol; half life; pyruvate decarboxylase; pyruvic acid; temperature
Abstract:
... Recent progress in enzymatic (R)-phenylacetylcarbinol (PAC) production has established the need for low cost and efficient biocatalyst preparation. Pyruvate decarboxylase (PDC) added in the form of Candida utilis cells showed higher stability towards benzaldehyde and temperature in comparison with partially purified preparations. In the presence of 50 mM benzaldehyde and at 4°C, a half-life of 228 ...
DOI:
10.1007/s00253-005-0063-1
PubMed:
16158280

29. Composition of the cell walls of several yeast species

Author:
Rogers, P.L.,   et al.   ; Nguyen, T.H.; Fleet, G.H.;     Show all 3 Authors
Source:
Applied microbiology and biotechnology 1998 v.50 no.2 pp. 206-212
ISSN:
0175-7598
Subject:
Debaryomyces hansenii; Kloeckera apiculata; Kluyveromyces marxianus; Saccharomyces cerevisiae; Zygosaccharomyces bailii; ammonium sulfate; cell walls; chitin; glucans; potassium hydroxide; viscosity; yeasts
Abstract:
... Cell walls, representing 26%-32% of the cell dry weight, were prepared from several strains of the yeasts Kloeckera apiculata, Debaryomyces hansenii, Zygosaccharomyces bailii, Kluyveromyces marxianus and Saccharomyces cerevisiae. Extraction of the walls with potassium hydroxide at 4 degrees C, followed by saturation of the alkali-soluble extract with ammonium sulphate gave fractions of mannoprotei ...
DOI:
10.1007/s002530051278
PubMed:
9763691

30. Reductive dechlorination of chlorophenols in slurries of low-organic-carbon marine sediments and subsurface soils

Author:
Rogers, J.E.,   et al.   ; Hale Boothe, D.D.; Wiegel, J.;     Show all 3 Authors
Source:
Applied microbiology and biotechnology 1997 v.47 no.6 pp. 742-748
ISSN:
0175-7598
Subject:
slurries; subsoiling; yeast extract; soil; chlorophenols; dissolved organic carbon; dechlorination; marine sediments
Abstract:
... The reductive dechlorination of 2,4- and 3,4-dichlorophenol (DCP) was studied in slurries of marine sediments and subsurface soils with dissolved organic carbon concentrations less than 1 ppm. Dechlorination was markedly greater in marine sediment slurries than in subsoil slurries, although similar products were observed in each case. From 25% to 98% of the 2,4- and 3,4-pop (6.5 micromoles/l) adde ...
DOI:
10.1007/s002530051004

31. A screen-printed biosensor using pyruvate oxidase for rapid determination of phosphate in synthetic wastewater

Author:
Kwan, Roger C. H.,   et al.   ; Leung, H. F.; Hon, Phoebe Y. T.; Barford, J. P.; Renneberg, R.;     Show all 5 Authors
Source:
Applied microbiology and biotechnology 2005 v.66 no.4 pp. 377-383
ISSN:
0175-7598
Subject:
biosensors; carbon dioxide; detection limit; electrodes; hydrocolloids; hydrogen peroxide; immobilized enzymes; monitoring; oxygen; phosphates; process control; pyruvate oxidase; pyruvic acid; silver chloride; wastewater
Abstract:
... A screen-printed phosphate biosensor based on immobilized pyruvate oxidase (PyOD, E.C. 1.2.3.3) has been developed for monitoring phosphate concentrations in a sequencing batch reactor (SBR) system. The enzyme was immobilized by a nafion matrix and covered a poly(carbamoyl) sulfonate (PCS) hydrogel on a screen-printed electrode. PyOD consumes phosphate in the presence of pyruvate and oxygen and ge ...
DOI:
10.1007/s00253-004-1701-8

32. Cloning and expression of the Bacillus sphaericus 2362 mosquitocidal genes in a non-toxic unicellular cyanobacterium, Synechococcus PCC6301

Author:
Rogers, P.L.,   et al.   ; Sangthongpitag, K.; Penfold, R.J.; Delaney, S.F.;     Show all 4 Authors
Source:
Applied microbiology and biotechnology 1997 v.47 no.4 pp. 379-384
ISSN:
0175-7598
Subject:
Bacillus sphaericus; Culex quinquefasciatus; Escherichia coli; Synechococcus; breeding; erythrocytes; genes; genetic vectors; insect larvae; insecticidal properties; lethal concentration 50; toxicity
Abstract:
... Genes encoding the mosquitocidal binary toxin of Bacillus sphaericus 2362 were introduced into Synechococcus PCC63O1, a cyanobacterium that can tolerate a number of potential variations in the mosquito breeding environment, and can serve as a food source for mosquito larvae. The toxin genes, preceded by a Synechococcus RBC promoter, were located on a mobilizable Escherichia coli Synechococcus shut ...
DOI:
10.1007/s002530050944
PubMed:
9163952