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- Lin, Jianping, et al. Show all 6 Authors
- Applied microbiology and biotechnology 2020 v.104 no.5 pp. 2039-2050
- Escherichia coli; amino acids; bacteria; batch fermentation; bioreactors; corn cobs; glucose; host strains; hydrolysates; lignocellulose; mutation; pollution; raw materials; toxins; xylitol; xylose
- ... The biotechnology-based production of xylitol has received widespread attention because it can use cheap and renewable lignocellulose as a raw material, thereby decreasing costs and pollution. The simultaneous use of various sugars in lignocellulose hydrolysates is a primary prerequisite for efficient xylitol production. In this study, a ΔptsG and crp* combinatorial strategy was used to generate E ...
- Lin, Jianping, et al. Show all 3 Authors
- Applied microbiology and biotechnology 2007 v.75 no.4 pp. 777-782
- biosynthesis; succinic acid; fermenters; glucose; codons; Rhodobacter sphaeroides; temperature; enzyme activity; Escherichia coli
- ... The 5-aminolevulinate (ALA) synthase gene (hemA) containing several codons rarely used by Escherichia coli was cloned from the genome of Rhodobacter sphaeroides and optimized in two strains of Escherichia coli: BL21(DE3) and Rosetta(DE3), which is a rare codon optimizer strain. The effects of initial isopropyl-β-D-thiogalactopyranoside (IPTG) concentration, induction time, and temperature on enzym ...