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- Selvaraj, Jonathan Nimal, et al. Show all 6 Authors
- Applied microbiology and biotechnology 2019 v.103 no.13 pp. 5231-5241
- Escherichia coli; Massilia; bacteria; biocatalysts; catalytic activity; cold; cost effectiveness; energy; genes; industry; mutational analysis; pH; pectate lyase; polygalacturonase; polygalacturonic acid; temperature; Antarctic region
- ... Cold-active enzymes have become attractive biocatalysts in biotechnological applications for their ability to retain high catalytic activity below 30 °C, which allows energy reduction and cost saving. Here, a 1041 bp gene pel1 encoding a 34.7 KDa pectate lyase was cloned from a facultatively psychrophilic Antarctic bacterium Massilia eurypsychrophila and heterologously expressed in Escherichia col ...