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- Tang, Xiaoqian, et al. Show all 6 Authors
- Aquaculture 2019 v.505 pp. 1-11
- Paralichthys olivaceus; Vibrio anguillarum; Western blotting; antibodies; bile; enzyme-linked immunosorbent assay; fish health; flounder; gene expression; gene expression regulation; gills; hindgut; immunoglobulin M; immunohistochemistry; intraperitoneal injection; kidneys; liver; messenger RNA; mucosa; mucosal immunity; mucus; polymers; quantitative polymerase chain reaction; secretion; skin (animal); spleen; vaccination; vaccines
- ... Polymeric immunoglobulins (Igs) and polymeric immunoglobulin receptor (pIgR) play crucial roles in teleost mucosal surface defenses, but their mucosal immune responses, especially under different routes of vaccine administration, needs to be better clarified. In the present study, the flounder (Paralichthys olivaceus) was immunized with inactivated Vibrio anguillarum by intraperitoneal injection a ...
- Tang, Xiaoqian, et al. Show all 4 Authors
- Aquaculture 2012 v.358-359 pp. 132-138
- Paralichthys olivaceus; cell membranes; flounder; fluorescent antibody technique; gel electrophoresis; gills; glycoproteins; mass spectrometry; membrane proteins; polyclonal antibodies; protein binding; rabbits; receptors; sodium; sugars; trypsin; viruses
- ... Flounder gill (FG) cell line derived from the gill tissue of Japanese flounder (Paralichthys olivaceus) was employed as a target cell for investigating the molecules involved in lymphocystis disease virus (LCDV) binding and infection. Indirect immunofluorescence assay showed that LCDV could attach to the FG cells membrane susceptibly. By virus overlay protein binding assay (VOPBA), a 37.6kDa LCDV- ...