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- Author:
- Tang, Xiaoqian, et al. ; Xing, Jing; Liu, Wenjing; Tan, Yanmiao; Zhan, Wenbin; Show all 5 Authors
- Source:
- Aquaculture 2019 pp. 734299
- ISSN:
- 0044-8486
- Subject:
- Azumapecten farreri; Escherichia coli; Staphylococcus aureus; Vibrio anguillarum; Western blotting; carbohydrates; fluorescent antibody technique; gills; hemocytes; hepatopancreas; innate immunity; lectins; molecular weight; monoclonal antibodies; muscles; pathogen-associated molecular patterns; protein content; receptors; recombinant proteins; scallops
- Abstract:
- ... C-type lectins, as types of pattern recognition receptors (PRRs), have crucial roles in innate immunity, and their conserved carbohydrate recognition domain (CRD) is recognized as the binding dominant to pathogen-associated molecular patterns (PAMPs). In this study, a C-type lectin in the scallop Chlamys farreri (lectin B, GenBank No. DQ209290) was heterologously expressed and the recombinant prot ...
- DOI:
- 10.1016/j.aquaculture.2019.734299
-
https://dx.doi.org/10.1016/j.aquaculture.2019.734299
- Author:
- Tang, Xiaoqian, et al. ; Wang, Xiaolu; Zhan, Wenbin; Show all 3 Authors
- Source:
- Aquaculture 2012 v.368-369 pp. 1-9
- ISSN:
- 0044-8486
- Subject:
- Escherichia coli; Fenneropenaeus chinensis; Pacifastacus leniusculus; Western blotting; amino acid sequences; amino acids; complementary DNA; enzyme-linked immunosorbent assay; epidermal growth factor; fluorescent antibody technique; gills; gonads; heart; hemocytes; hepatopancreas; intestines; isoelectric point; messenger RNA; muscles; neutralization; polyclonal antibodies; polymerase chain reaction; proteins; races; recombinant antibodies; shrimp
- Abstract:
- ... An integrin β subunit (FcβInt) cDNA was cloned from hemocytes of Fenneropenaeus chinensis by rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of FcβInt consists of 2853bp and encodes a polypeptide of 783 amino acids with a predicted isoelectric point of 5.09. The deduced amino acid sequence of FcβInt possessed the classical domains of β integrin, including the βA domain and four e ...
- DOI:
- 10.1016/j.aquaculture.2012.08.004
-
http://dx.doi.org/10.1016/j.aquaculture.2012.08.004
- Author:
- Tang, Xiaoqian, et al. ; Lin, Tingting; Xing, Jing; Jiang, Jingwei; Zhan, Wenbin; Show all 5 Authors
- Source:
- Aquaculture 2011 v.315 no.3-4 pp. 213-221
- ISSN:
- 0044-8486
- Subject:
- Chlamys; acid phosphatase; alkaline phosphatase; enzyme activation; enzyme activity; hemocytes; immune system; mortality; myeloperoxidase; peroxidase; scallops; superoxide dismutase; water temperature; Alps region
- Abstract:
- ... High water temperatures are associated with immune suppression and elevated mortality in economically important marine bivalves. To determine whether the stimulatory effects of β-glucan on enzymes associated with the immune system were depressed at higher temperatures, scallops Chlamys farreri were treated with 0.5, 1.0 or 2.0mg/ml of β-glucan suspensions at 15°C or 25°C, and the activities of six ...
- DOI:
- 10.1016/j.aquaculture.2011.02.034
-
http://dx.doi.org/10.1016/j.aquaculture.2011.02.034
- Author:
- Tang, Xiaoqian, et al. ; Liu, Baoliang; Zhan, Wenbin; Show all 3 Authors
- Source:
- Aquaculture 2011 v.314 no.1-4 pp. 13-17
- ISSN:
- 0044-8486
- Subject:
- Fenneropenaeus chinensis; White spot syndrome virus; cell membranes; digoxigenin; fluorescein; fluorescence; hemocytes; host-pathogen relationships; hosts; infection; molecular weight; protein binding; shrimp; structural proteins; viral proteins; viruses
- Abstract:
- ... Hemocyte of shrimp as an important target cell for white spot syndrome virus (WSSV) was an optimal target for the investigation of interactions between WSSV structural proteins and host cells. In this study, hemocyte membrane of Fenneropenaeus chinensis (HmFc) was prepared and labeled with digoxigenin (DIG), then used as a probe to detect the HmFc-binding proteins in WSSV structural proteins by mo ...
- DOI:
- 10.1016/j.aquaculture.2011.01.023
-
http://dx.doi.org/10.1016/j.aquaculture.2011.01.023