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- Author:
- Rioboo, Carmen; O'Connor, José Enrique; Prado, Raquel; Herrero, Concepción; Cid, Ángeles
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 229-237
- ISSN:
- 0166-445X
- Subject:
- microalgae; Chlorella vulgaris; terbutryn; cytotoxicity; cell proliferation; viability; microbial growth; cytokinesis
- Abstract:
- ... Very little is known about growth and proliferation in relation to the cell cycle regulation of algae. The lack of knowledge is even greater when referring to the potential toxic effects of pollutants on microalgal cell division. To assess the effect of terbutryn, a triazine herbicide, on the proliferation of the freshwater microalga Chlorella vulgaris three flow cytometric approaches were used: ( ...
- DOI:
- 10.1016/j.aquatox.2009.07.009
- PubMed:
- 19679360
-
http://dx.doi.org/10.1016/j.aquatox.2009.07.009
- Author:
- Chen, Dong-Shi; Chan, King Ming
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 163-176
- ISSN:
- 0166-445X
- Subject:
- Oreochromis niloticus; tilapia (common name); copper; cytotoxicity; toxicity testing; metabolic detoxification; liver; cell lines; hepatocytes; proteins; gene expression; polymerase chain reaction; lethal concentration 50
- Abstract:
- ... Copper is an essential element in a variety of biological processes, but it can be toxic when present in excessive amounts. The central regulators of cellular copper metabolism include copper-binding proteins, copper transporters, metal membrane active transporters and copper-dependent enzymes. However, the way in which cupric ions (Cu²⁺⁾ cause cellular changes in proteins and lead to toxic effect ...
- DOI:
- 10.1016/j.aquatox.2009.06.014
-
http://dx.doi.org/10.1016/j.aquatox.2009.06.014
- Author:
- Tryfonos, Melpomeni; Papaefthimiou, Chrisovalantis; Antonopoulou, Efthimia; Theophilidis, George
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 211-218
- ISSN:
- 0166-445X
- Subject:
- water pollution; organophosphorus pesticides; toxicity testing; heart; bream; Sparus aurata; cholinergic receptors
- Abstract:
- ... Organophosphates (OPs) can provoke toxicity by inhibiting acetylcholinesterase (AChE) in non-target organisms, like fish. In a previous pilot study, the anticholinesterase effects of paraoxon on the heart of Sparus aurata were examined [Tryfonos, M., Antonopoulou, E., Papaefthimiou, C., Chaleplis, G., Theophilidis, G., 2009. An in vitro assay for the assessment of the effects of an organophosphate ...
- DOI:
- 10.1016/j.aquatox.2009.07.003
- PubMed:
- 19674799
-
http://dx.doi.org/10.1016/j.aquatox.2009.07.003
- Author:
- Monteiro, Sandra Mariza; dos Santos, Nuno M.S.; Calejo, Margarida; Fontainhas-Fernandes, António; Sousa, Mário
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 219-228
- ISSN:
- 0166-445X
- Subject:
- water pollution; copper; cytotoxicity; Oreochromis niloticus; tilapia (common name); gills; apoptosis; cell proliferation; DNA fragmentation; messenger RNA; gene expression; gene induction
- Abstract:
- ... Recent in vitro studies have demonstrated that copper may induce apoptosis triggering the activation of caspase-3, a central effector of apoptotic cell death. However, the precise mechanism of copper-induced apoptosis is still unclear, even less so in Oreochromis niloticus where no caspase genes have been reported so far. This study aimed to assess the in vivo role of copper in apoptosis induction ...
- DOI:
- 10.1016/j.aquatox.2009.07.008
- PubMed:
- 19656581
-
http://dx.doi.org/10.1016/j.aquatox.2009.07.008
- Author:
- Dang, Fei; Zhong, Huan; Wang, Wen-Xiong
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 238-244
- ISSN:
- 0166-445X
- Subject:
- Acanthopagrus; marine fish; water pollution; copper; dietary exposure; environmental exposure; acclimation; kinetics; bioaccumulation; metallothionein; uptake mechanisms
- Abstract:
- ... The uptake kinetics and regulation of copper in a marine predatory fish, the black sea bream Acanthopagrus schlegeli after acclimation to waterborne Cu were examined, using radiotracer techniques. The dissolved Cu uptake followed a linear pattern during the time of exposure, and the calculated uptake rate constant was 6.24Lkg⁻¹ day⁻¹. The efflux rate constant was 0.091day⁻¹ following dietary uptak ...
- DOI:
- 10.1016/j.aquatox.2009.07.011
- PubMed:
- 19683350
-
http://dx.doi.org/10.1016/j.aquatox.2009.07.011
- Author:
- Martin-Diaz, Laura; Franzellitti, Silvia; Buratti, Sara; Valbonesi, Paola; Capuzzo, Antonio; Fabbri, Elena
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 177-185
- ISSN:
- 0166-445X
- Subject:
- drugs; environmental exposure; Mytilus galloprovincialis; mussels; biomarkers; oxidative stress; enzyme activity; cyclic AMP; messenger RNA; gene expression; protein kinases
- Abstract:
- ... Pharmaceuticals represent a diverse collection of thousands of bioactive chemicals used in human and veterinary medicine. The increased consumption, together with the recent development of more sensitive analytical techniques, has identified these as emerging contaminants in the aquatic environment. According to many investigations pharmaceuticals do not cause acute toxic effects in organisms when ...
- DOI:
- 10.1016/j.aquatox.2009.06.015
- PubMed:
- 19632730
-
http://dx.doi.org/10.1016/j.aquatox.2009.06.015
- Author:
- Bickley, L.K.; Lange, A.; Winter, M.J.; Tyler, C.R.
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 195-203
- ISSN:
- 0166-445X
- Subject:
- endocrine-disrupting chemicals; estradiol; estrogenic properties; Cyprinus carpio; carp; hepatocytes; in vitro culture; vitellogenin; vitellogenesis; messenger RNA; gender differences; gonads; histology; cytotoxicity; viability
- Abstract:
- ... The presence of endocrine disrupting chemicals (EDCs) in the environment has driven the development of screening and testing assays to both identify chemicals with hormonal activity and evaluate their potential to cause adverse effects. As the number of animals used for research and regulatory purposes rises, and set against a desire to reduce animal testing, there is increased emphasis on the dev ...
- DOI:
- 10.1016/j.aquatox.2009.07.006
- PubMed:
- 19695720
-
http://dx.doi.org/10.1016/j.aquatox.2009.07.006
- Author:
- Saby, Emilie; Justesen, Just; Kelve, Merike; Uriz, Maria J.
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 204-210
- ISSN:
- 0166-445X
- Subject:
- water pollution; heavy metals; Porifera; enzyme activity; enzyme inhibition; immune response; immunotoxicity; Mediterranean Sea
- Abstract:
- ... Heavy metals are among the main pollutants of the Mediterranean coastal waters where they can harm sublittoral biota. Filter-feeder, long-living invertebrates that remain fixed to the rocky bottom, such as sponges, are good targets to metal contamination studies since they may be exposed to potential low levels of contamination for years. Several molecular and biochemical mechanisms are developed ...
- DOI:
- 10.1016/j.aquatox.2009.07.002
- PubMed:
- 19651447
-
http://dx.doi.org/10.1016/j.aquatox.2009.07.002
- Author:
- Fitzsimmons, Patrick N.; Hoffman, Alex D.; Lien, Gregory J.; Hammermeister, Dean E.; Nichols, John W.
- Source:
- Aquatic toxicology 2009 v.94 no.3 pp. 186-194
- ISSN:
- 0166-445X
- Subject:
- water pollution; dichloroacetic acid; disinfectants; byproducts; Oncorhynchus mykiss; trout; fish; toxicity testing; pharmacokinetics; biochemistry; physiological response
- Abstract:
- ... Halogenated acetic acids (HAAs) produced by chlorine disinfection of municipal drinking water represent a potentially important class of environmental contaminants. Little is known, however, about their potential to adversely impact fish and other aquatic life. In this study we examined the kinetics and effects of dichloroacetic acid (DCA) in rainbow trout. Branchial uptake was measured in fish co ...
- DOI:
- 10.1016/j.aquatox.2009.07.001
- PubMed:
- 19656582
-
http://dx.doi.org/10.1016/j.aquatox.2009.07.001