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- Fordwour, Osei Boakye; Wolthers, Kirsten R.
- Archives of biochemistry and biophysics 2018 v.654 pp. 85-96
- nicotinamide; lactones; NADP (coenzyme); models; reducing agents; cyclohexanones; phosphates; active sites; hydrogen bonding; spectral analysis; Acinetobacter; catalytic activity; oxygen; ribose
- ... Baeyer-Villiger monooxygenases are flavoenzymes that use NADPH and O2 to convert ketones to esters or lactones. A diagnostic feature of BVMO catalysis is the dual role of the pyridine nucleotide: NADPH functions as a reductant of the FAD cofactor and the resulting NADP+ acts to stabilize the ensuing C4a-peroxyflavin intermediate. Using cyclohexanone monooxygenase from Acinetobacter sp. NCIMB 9871 ...