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1. Preliminary results from structural systems biology approach in Tetrahymena thermophila reveal novel perspectives for this toxicological model

Author:
Chasapis, Christos T.
Source:
Archives of microbiology 2019 v.201 no.1 pp. 51-59
ISSN:
0302-8933
Subject:
Tetrahymena thermophila; cadmium; databases; eukaryotic cells; genes; human diseases; models; protein-protein interactions; proteins; topology; toxicity; toxicology; yeasts
Abstract:
... Tetrahymena is a unicellular microbial eukaryotic organism that has been used extensively in toxicology and environmental research. This work attempts to model for the first time the wiring of proteins involved in cellular mechanisms of Cd toxicity in Tetrahymena thermophila. 1975 high-confidence PPIs between 68 Cd-binding proteins and 422 partners were inferred through a novel structural systems ...
DOI:
10.1007/s00203-018-1571-6

2. Regulation of the inositol transporter Itr1p by hydrogen peroxide in Saccharomyces cerevisiae

Author:
Santos, Tomás; Marinho, H. Susana; Cyrne, Luisa
Source:
Archives of microbiology 2019 v.201 no.1 pp. 123-134
ISSN:
0302-8933
Subject:
Saccharomyces cerevisiae; culture media; genes; hydrogen peroxide; myo-inositol; phenotype; phospholipids; plasma membrane; sphingolipids; transporters; yeasts
Abstract:
... Myo-inositol is a precursor of several membrane phospholipids and sphingolipids and plays a key role in gene regulation in Saccharomyces cerevisiae (S. cerevisiae). Here, we tested whether H₂O₂ was affecting the levels of the inositol transporters and thus inositol uptake. In S. cerevisiae cells adapted to H₂O₂ Itr1–GFPp accumulated in the plasma membrane until 20 min, concomitantly with an inhibi ...
DOI:
10.1007/s00203-018-1584-1

3. Phenotypical characterisation of a putative ω-amino acid transaminase in the yeast Scheffersomyces stipitis

Author:
Linder, Tomas
Source:
Archives of microbiology 2019 v.201 no.2 pp. 185-192
ISSN:
0302-8933
Subject:
Lachancea; Saccharomyces cerevisiae; Scheffersomyces stipitis; butyric acid; gamma-aminobutyric acid; genes; metabolism; mutants; nitrogen; phylogeny; transaminases; yeasts
Abstract:
... Phylogenetic analysis of class III transaminases in the budding yeasts Lachancea kluyveri, Saccharomyces cerevisiae and Scheffersomyces stipitis identified a hitherto uncharacterised Sch. stipitis transaminase encoded by the PICST_54153 gene, which clustered with previously described γ-amino butyric acid (GABA) and β-alanine transaminases. Deletion of the PICST_54153 gene in Sch. stipitis resulted ...
DOI:
10.1007/s00203-018-1608-x
PubMed:
30519708
PubMed Central:
PMC6514085

4. Transcriptome and metabolome analysis of Pichia stipitis to three representative lignocellulosic inhibitors

Author:
Zhu, Yuanyuan; Wu, Lu; Zhu, Junjun; Xu, Yong; Yu, Shiyuan
Source:
Archives of microbiology 2019 v.201 no.5 pp. 581-589
ISSN:
0302-8933
Subject:
Scheffersomyces stipitis; acetic acid; amino acid metabolism; asparagine; biomarkers; biotransformation; carbohydrate metabolism; ethanol; fatty acids; fermentation; gas chromatography-mass spectrometry; gene expression regulation; genes; glutamine; hydrolysates; hydroxymethylfurfural; lignocellulose; metabolites; metabolomics; principal component analysis; transcriptome; transcriptomics; vanillin; xylose; yeasts
Abstract:
... During the bioconversion of xylose to ethanol, Pichia stipitis cells are often inhibited by substances generated in the lignocellulosic hydrolysate. However, the response mechanism of P. stipitis to inhibitors has not been completely understood till date. With this aim, integrated transcriptomic and metabolomic analyses were performed on P. stipitis to investigate the interactive effects of three ...
DOI:
10.1007/s00203-018-1600-5