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- Mertens, J.A.; Skory, C.D.; Ibrahim, A.S.
- Archives of microbiology 2006 v.186 no.1 pp. 41
- recombinant DNA, etc ; Rhizopus oryzae; genetically engineered microorganisms; genetic transformation; plasmid vectors; promoter regions; glucan 1,4-alpha-glucosidase; pyruvate decarboxylase; phosphoglycerate kinase; reporter genes; green fluorescent protein; gene expression; messenger RNA; Show all 13 Subjects
- ... Rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. In this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae. Three plasmid vectors have been created using either the glucoamylase A (amyA), pyruvate decarboxylase ...