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- Ng, Wing-On; Zentella, Rodolfo; Wang, Yinsheng; Taylor, John-Stephen A.; Pakrasi, Himadri B.
- Archives of microbiology 2000 v.173 no.5-6 pp. 412-417
- histidine, etc ; Escherichia coli; Synechococcus; Synechocystis; absorption; flavin-adenine dinucleotide; gene overexpression; genes; mass spectrometry; plasmids; Show all 10 Subjects
- ... A new broad-host-range plasmid, pSL1211, was constructed for the over-expression of genes in Synechocystis sp. strain PCC 6803. The plasmid was derived from RSF1010 and an Escherichia coli over-expression plasmid, pTrcHisC. Over-expressed protein is made with a removable N-terminal histidine tag. The plasmid was used to over-express the phrA gene and purify the gene product from Synechocystis sp. ...