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- Comerci, Diego J., et al. Show all 9 Authors
- Archives of microbiology 2009 v.191 no.7 pp. 571-581
- Brucella melitensis biovar Abortus; Western blotting; bacteria; electrophoresis; genes; monoclonal antibodies; mutants; mutation; peptidylprolyl isomerase; type IV secretion system; virulence
- ... The virB genes coding type IV secretion system are necessary for the intracellular survival and replication of Brucella spp. In this study, extracellular proteins from B. abortus 2308 (wild type, WT) and its isogenic virB10 polar mutant were compared. Culture supernatants harvested in the early stationary phase were concentrated and subjected to 2D electrophoresis. Spots present in the WT strain b ...