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- Author:
- Xu, Peng-Li; Zhao, Yu; Zheng, Hui-Hua; Tian, Run-Bo; Han, Hao-Ying; Chen, Hong-Ying; Zheng, Lan-Lan
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1445-1451
- ISSN:
- 0304-8608
- Subject:
- Porcine circovirus-2; farms; genetic variation; genome; genotype; livestock and meat industry; mixed infection; molecular cloning; molecular epidemiology; polymerase chain reaction; sequence analysis; swine; China
- Abstract:
- ... In order to investigate the genetic diversity of porcine circovirus type 2 (PCV2), 284 clinical tissue samples were collected from different pig farms in central China from 2015 to 2017. A total of 162 tissue samples (162/284, 57.04%) were positive for PCV2 by PCR, and subsequently, the complete genome of 36 of these PCV2 samples was cloned and sequenced. The sequencing results showed that 37 comp ...
- DOI:
- 10.1007/s00705-019-04205-0
-
https://dx.doi.org/10.1007/s00705-019-04205-0
- Author:
- Romero Romero, J. Lucina; Carver, Gavriela Dena; Arce Johnson, Patricio; Perry, Keith L.; Thompson, Jeremy R.
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1453-1457
- ISSN:
- 0304-8608
- Subject:
- Grapevine red blotch virus; loop-mediated isothermal amplification; nucleic acids; polymerase chain reaction; viruses; viticulture; North America
- Abstract:
- ... Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide th ...
- DOI:
- 10.1007/s00705-019-04207-y
-
https://dx.doi.org/10.1007/s00705-019-04207-y
- Author:
- Zhang, Liping; Liu, Xinsheng; Zhang, Qiaoling; Zhou, Peng; Fang, Yuzhen; Dong, Zhaoliang; Zhao, Donghong; Li, Weiyan; Feng, Jiaxin; Zhang, Yongguang; Wang, Yonglu
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1287-1295
- ISSN:
- 0304-8608
- Subject:
- Porcine epidemic diarrhea virus; diarrhea; genes; genotype; mutation; phylogeny; piglets; pork industry; suckling; swine diseases; vaccines; virulence; China; United States
- Abstract:
- ... Since 2010, continual outbreaks of highly virulent variants of porcine epidemic diarrhea virus (PEDV) belonging to genotype GII have led to serious economic losses for the Chinese swine industry. To better understand the biological characteristics and pathogenicity of the current prevalent Chinese PEDV field strains, in this study, a highly virulent Chinese genotype GIIa PEDV strain, CH/HBXT/2018, ...
- DOI:
- 10.1007/s00705-019-04167-3
- PubMed:
- 30859476
- PubMed Central:
- PMC7086859
-
https://dx.doi.org/10.1007/s00705-019-04167-3
- Author:
- Chen, Ling; Guan, Guoye; Liu, Quan; Yuan, Shengjian; Yan, Tingwei; Tian, Linyu; Zhou, Yan; Zhao, Yixuan; Ma, Yingfei; Wei, Ting; Fu, Xiongfei
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1475-1478
- ISSN:
- 0304-8608
- Subject:
- Ackermannviridae; Salmonella enterica; bacteriophages; databases; genes; genomics; new species; open reading frames; phylogeny; proteins; sequence alignment; transmission electron microscopy
- Abstract:
- ... Salmonella phages SenALZ1 and SenASZ3, two novel phages infecting Salmonella enterica, were isolated and analyzed. The genomes of these two phages consist of 154,811 and 157,630 base pairs (bp), with G+C contents of 44.56% and 44.74%, respectively. Fifty-nine of 199 open reading frames (ORFs) in the SenALZ1 genome, and 60 of the 204 in the SenASZ3 genome show similarity to reference sequences in t ...
- DOI:
- 10.1007/s00705-019-04183-3
-
https://dx.doi.org/10.1007/s00705-019-04183-3
- Author:
- Kajsík, Michal; Bugala, Juraj; Kadličeková, Veronika; Szemes, Tomáš; Turňa, Ján; Drahovská, Hana
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1383-1391
- ISSN:
- 0304-8608
- Subject:
- Cronobacter; DNA-directed RNA polymerase; bacteriophages; genes; host specificity; infant formulas; neonates; nucleotide sequences; pathogens; phylogeny; sequence analysis; virulent strains
- Abstract:
- ... Cronobacter spp. are opportunistic pathogenic bacteria responsible for severe infections in neonates. Powdered infant formula has been confirmed to be the source of infection in some cases. Bacteriophages offer a safe means for eliminating this pathogen. In the present study, we characterized two closely related Cronobacter-specific bacteriophages of the proposed genus “GAP227virus”. The phages De ...
- DOI:
- 10.1007/s00705-019-04202-3
-
https://dx.doi.org/10.1007/s00705-019-04202-3
- Author:
- Popova, Anastasia V.; Shneider, Mikhail M.; Myakinina, Vera P.; Bannov, Vasily A.; Edelstein, Mikhail V.; Rubalskii, Evgenii O.; Aleshkin, Andrey V.; Fursova, Nadezhda K.; Volozhantsev, Nikolay V.
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1493-1497
- ISSN:
- 0304-8608
- Subject:
- Acinetobacter baumannii; DNA; DNA replication; Myoviridae; bacteria; bacteriophages; capsid; carbohydrate structure; enzyme activity; genes; metabolism; multiple drug resistance; open reading frames; polysaccharides; structural proteins; transmission electron microscopy; virulence; virus assembly
- Abstract:
- ... In the present study, we investigate the biological properties and genomic organization of virulent bacteriophage AM24, which specifically infects multidrug-resistant clinical Acinetobacter baumannii strains with a K9 capsular polysaccharide structure. The phage was identified as a member of the family Myoviridae by transmission electron microscopy. The AM24 linear double-stranded DNA genome of 97 ...
- DOI:
- 10.1007/s00705-019-04208-x
-
https://dx.doi.org/10.1007/s00705-019-04208-x
- Author:
- Zhu, Wei; Haga, Takeshi; Yuan, Dongfang; Watanabe, Ken-ichi; Chambers, James K.; Gao, Nannan; Uchida, Kazuyuki; Fan, Shushan; Li, Fang; Yang, Mingcai; Norimine, Junzo; Hu, Shilin; Dong, Jianbao
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1441-1444
- ISSN:
- 0304-8608
- Subject:
- Bovine papular stomatitis virus; Papillomaviridae; cows; epidemiological studies; histopathology; immunohistochemistry; mixed infection; papilloma; reverse transcriptase polymerase chain reaction; slaughterhouses; tongue; viruses
- Abstract:
- ... To date, there have been no reports of coinfection with bovine papular stomatitis virus (BPSV) and bovine papillomavirus (BPV) in the same lesion. In the present study, one lingual papilloma-like sample was collected at an abattoir from the tongue of a 31-month-old Japanese black cow. Coinfection with BPSV and BPV was confirmed by histopathology, immunohistochemistry, PCR and RT-PCR. The evidence ...
- DOI:
- 10.1007/s00705-019-04199-9
-
https://dx.doi.org/10.1007/s00705-019-04199-9
- Author:
- Nordmann, Birthe; Schilling, Tobias; Hoppert, Michael; Hertel, Robert
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1485-1488
- ISSN:
- 0304-8608
- Subject:
- Bacillus thuringiensis; DNA; Myoviridae; bacteriophages; biological control agents; chromosomes; databases; genome; genomics; humans; open reading frames; transfer RNA; transmission electron microscopy; viral morphology
- Abstract:
- ... Bacillus thuringiensis (Bt) is non-pathogenic for humans and serves as a biological control agent in agriculture. Understanding its phages will help to prevent industrial production loss of Bt products and will lead to a better understanding of phages in general. The complete genome of the new B. thuringiensis phage isolate vB_BthM-Goe5 (Goe5) was sequenced, revealing a linear 157,804-bp-long dsDN ...
- DOI:
- 10.1007/s00705-019-04187-z
-
https://dx.doi.org/10.1007/s00705-019-04187-z
- Author:
- Peng, Ting-Ting; Cai, Qiu-E; Yang, Min; Chen, Shao-Ping; Chen, Feng; Wang, Miao; Peng, Ling; Wong, Gary; Shen, Chen-Guang; Cheng, Wen-Bing; Liu, Sai-Yun; Peng, Ya-Bai; Peng, Jing-Han; Chen, Chu-Ming; Yang, Liu-Qing; Tang, Yi-Min; Xu, Zhi-Xiang; Liu, Ying-Xia
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1335-1341
- ISSN:
- 0304-8608
- Subject:
- DNA; Hepatitis B virus; blood; blood sampling; hepatitis B; hepatitis B antigens; hospitals; mothers; neonates; pregnant women; progeny; risk factors; umbilical cord; vertical disease transmission; China
- Abstract:
- ... In infants, hepatitis B virus (HBV) infections are mainly acquired by mother-to-child transmission (MTCT). Current tests for the presence of HBV markers at birth can neither confirm nor exclude MTCT. The aim of this study was to find an early diagnostic marker of HBV MTCT. From 2011 to 2016, we studied a total of 5999 pregnant women who gave birth at our hospital in Shenzhen City, China. HBsAg-pos ...
- DOI:
- 10.1007/s00705-019-04190-4
-
https://dx.doi.org/10.1007/s00705-019-04190-4
- Author:
- Zhang, Lingling; Gao, Dou; Yu, Yongle; Liu, Yebing; Liu, Weiquan; Li, Jinxiang; Cui, Shangjin
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1459-1467
- ISSN:
- 0304-8608
- Subject:
- Ungulate protoparvovirus 1; genetic markers; pathogenesis; rapid methods; viruses
- Abstract:
- ... In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms ...
- DOI:
- 10.1007/s00705-019-04209-w
-
https://dx.doi.org/10.1007/s00705-019-04209-w
- Author:
- Farias, Karla P. R. A.; Moreli, Marcos L.; Floriano, Vitor G.; da Costa, Vivaldo G.
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1249-1257
- ISSN:
- 0304-8608
- Subject:
- Human betaherpesvirus 5; algorithms; brain neoplasms; computer software; confidence interval; etiology; glioma; histology; meta-analysis; models; nucleic acids; observational studies; pathogenesis; patients; risk factors
- Abstract:
- ... Malignant gliomas are the most common types of incurable primary brain tumours. Therefore, to better clarify the aetiology and pathogenesis of the disease and analyse the risk factors involved, several researchers have highlighted a possible link to human cytomegalovirus (HCMV). Regarding this potential link, the numbers of studies and controversies concerning the relationship between HCMV infecti ...
- DOI:
- 10.1007/s00705-019-04206-z
-
https://dx.doi.org/10.1007/s00705-019-04206-z
- Author:
- Zhao, Chengxue; Chen, Chen; Li, Ying; Dong, Shuren; Tan, Kun; Tian, Yuhui; Zhang, Lilin; Huang, Jinhai; Zhang, Lei
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1469-1473
- ISSN:
- 0304-8608
- Subject:
- Mamastrovirus 3; amino acid sequences; coat proteins; diarrhea; evolution; feces; genetic recombination; genetic variation; genome; genomics; herds; piglets; promoter regions; reverse transcriptase polymerase chain reaction; sequence analysis; viruses; China
- Abstract:
- ... Porcine astroviruses (PAstVs), are widely distributed viruses that are highly prevalent in swine herds. In this study, a novel type 4 porcine astrovirus strain (designated as PAstV4/Tianjin/2018) was identified in a fecal sample from a diarrheal piglet in Tianjin, China and its complete genomic sequence was determined by RT-PCR. Sequence analysis showed that this strain had a capsid protein with a ...
- DOI:
- 10.1007/s00705-019-04162-8
-
https://dx.doi.org/10.1007/s00705-019-04162-8
- Author:
- Paul, Catherine; Khera, Lohit; Kaul, Rajeev
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1271-1285
- ISSN:
- 0304-8608
- Subject:
- Hepatitis C virus; cell movement; etiological agents; genotype; hepatoma; humans; males; metastasis; neoplasm cells; sumoylation; transcription (genetics); viral core proteins
- Abstract:
- ... Hepatitis C virus (HCV) is the major etiological agent of hepatocellular carcinoma (HCC), which is the fourth most common cause of cancer-related deaths worldwide and second in terms of deaths of males (Bray et al. in CA Cancer J Clin 68(6):394–424, 2018). HCV-induced HCC is a multi-step process that involves alteration of several host regulatory pathways. One of the key features of HCV-associated ...
- DOI:
- 10.1007/s00705-019-04151-x
-
https://dx.doi.org/10.1007/s00705-019-04151-x
- Author:
- Cantelli, Carina Pacheco; da Silva, Marcelle Figueira Marques; Fumian, Tulio Machado; da Cunha, Denise Cotrim; Andrade, Juliana da Silva Ribeiro de; Malta, Fábio Correia; da Silva e Mouta Junior, Sérgio; Fialho, Alexandre Madi; de Moraes, Marcia Terezinha Baroni; Brasil, Patricia; Miagostovich, Marize Pereira; Leite, José Paulo Gagliardi
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1427-1432
- ISSN:
- 0304-8608
- Subject:
- Norovirus; children; feces; genetic variation; genotype; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sequence analysis; urban areas; Brazil
- Abstract:
- ... We report on the occurrence and diversity of noroviruses in children (younger than 5 years old of age) from a low-income urban area in Rio de Janeiro, Brazil. Sixty-one stool specimens collected from children between 1 and 4 years old with acute diarrhoeic episodes (ADE) and non-ADE were investigated. RT-qPCR and sequencing of PCR products after conventional RT-PCR analysis were performed. Norovir ...
- DOI:
- 10.1007/s00705-019-04195-z
-
https://dx.doi.org/10.1007/s00705-019-04195-z
- Author:
- Du, Juan; Zhu, Teng; Zhuang, Lu; Zhang, Pan-He; Zhang, Xiao-Ai; Lu, Qing-Bin; Liu, Wei
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1489-1492
- ISSN:
- 0304-8608
- Subject:
- Human enterovirus; boys; children; genome; phylogeny; pneumonia; China; United States
- Abstract:
- ... In this study, human enterovirus C117 (EV-C117) was detected in a 3-month-old boy diagnosed with pneumonia in China. A phylogenetic analysis showed that this strain was genetically closer to the Lithuanian strain than to the USA strain. ...
- DOI:
- 10.1007/s00705-019-04196-y
- PubMed:
- 30877454
- PubMed Central:
- PMC7086829
-
https://dx.doi.org/10.1007/s00705-019-04196-y
- Author:
- Yu, Fei; Wang, Hao; Wang, Longlong; Li, Wanjuan; Lu, Liqun
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1393-1404
- ISSN:
- 0304-8608
- Subject:
- Aquareovirus; Ctenopharyngodon idella; DNA viruses; Retroviridae; gene expression; genomics; regulatory proteins; reporter genes; transcription (genetics); transcription factors; transcriptional activation; viral nonstructural proteins; yeasts
- Abstract:
- ... Many transcription factors are encoded by DNA viruses and retroviruses due to their regulatory roles in gene expression in the host cell. However, no transcriptional regulator has been identified in any reovirus. Here, a non-structural protein, NS31, encoded by grass carp reovirus genomic segment S7 was characterized. The NS31 protein is predicted to contain a helix-turn-helix (HTH)-like domain an ...
- DOI:
- 10.1007/s00705-019-04204-1
-
https://dx.doi.org/10.1007/s00705-019-04204-1
- Author:
- Himeno, Ai; Ishida, Yuki; Mori, Hiromi; Matsuura, Kanako; Kikukawa, Minako; Sakawaki, Hiromi; Miura, Tomoyuki
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1297-1308
- ISSN:
- 0304-8608
- Subject:
- CCR5 receptor; CXCR4 receptor; Human immunodeficiency virus 1; Macaca mulatta; clones; models; mutation; mutational analysis; neutralization; neutralizing antibodies; vaccines; viruses
- Abstract:
- ... We previously developed CCR5-tropic neutralization-resistant simian/human immunodeficiency virus (SHIV) strains and a rhesus macaque model of infection with these SHIVs. We induced the production of neutralizing antibodies (nAbs) against HIV-1 by infecting rhesus macaques with different neutralization-resistant SHIV strains. First, SHIV-MK1 (MK1) (neutralization susceptible, tier 1B) with CCR5 tro ...
- DOI:
- 10.1007/s00705-019-04173-5
- PubMed:
- 30820667
- PubMed Central:
- PMC6469619
-
https://dx.doi.org/10.1007/s00705-019-04173-5
- Author:
- Bansode, Yashvant D.; Chattopadhyay, Debprasad; Saha, Bhaskar
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1433-1439
- ISSN:
- 0304-8608
- Subject:
- DNA; Human alphaherpesvirus 1; RNA; Toll-like receptors; astrocytes; central nervous system; epithelium; gene expression regulation; genes; immune response; innate immunity; interferons; pathogens; ribonucleases
- Abstract:
- ... Herpes simplex virus 1 (HSV-1), a double-stranded DNA virus, infects epithelial surfaces and establishes latency in the central nervous system, where astrocytes are a major immune cell type. Here, we report changes that occur in the expression of pathogen recognition receptors, such as Toll-like receptors, DNA and RNA sensors, interferons, and interferon-stimulated genes, when astrocytes are infec ...
- DOI:
- 10.1007/s00705-019-04197-x
-
https://dx.doi.org/10.1007/s00705-019-04197-x
- Author:
- Mori, Hiroshi; Tomiyasu, Takafumi; Nishiyama, Kanako; Matsumoto, Maiko; Osawa, Yoshiaki; Okazaki, Katsunori
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1343-1351
- ISSN:
- 0304-8608
- Subject:
- Bovine leukemia virus; amino acids; angiogenesis; blood vessels; carcinogenesis; cattle; cell lines; chemoattractants; chemotaxis; fibroblasts; human umbilical vein endothelial cells; mice; mutation; neoplasm cells; neoplasms; rats; stem cells; viruses
- Abstract:
- ... Bovine leukemia virus (BLV) can be divided into two categories based on the amino acid at position 233 in the Tax protein, which probably plays a crucial role in leukemogenesis. We show here that a rat fibroblast cell line stably expressing L233-Tax formed significantly larger tumors than P233-Tax-expressing cells in a murine xenograft study. Although the microvessel density was comparable in both ...
- DOI:
- 10.1007/s00705-019-04191-3
-
https://dx.doi.org/10.1007/s00705-019-04191-3
- Author:
- Cottet, Luis; Potgieter, Christiaan A.; Castro, Miguel E.; Castillo, Antonio
- Source:
- Archives of virology 2019 v.164 no.5 pp. 1479-1483
- ISSN:
- 0304-8608
- Subject:
- Alternaria; Botrytis cinerea; RNA-directed RNA polymerase; amino acid sequences; double-stranded RNA; electrophoresis; fungi; genome; nucleotides; open reading frames; phylogeny; polypeptides; sequence analysis; viruses
- Abstract:
- ... Eight different double-stranded RNA (dsRNA) molecules were found in the wild-type fungal strain Botrytis cinerea CCg427. The electrophoretic profile displayed molecules with approximate sizes of 1, 1.3, 1.6, 1.8, 3.3, 4.1, 6.5, and 12 kbp. Sequences analysis of the molecules in the 6.5-kbp band revealed the presence of two different dsRNA molecules (dsRNA-1 and dsRNA-2) of 6192 and 5567 bp. Each m ...
- DOI:
- 10.1007/s00705-019-04184-2
-
https://dx.doi.org/10.1007/s00705-019-04184-2