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- Author:
- Lim, H. Y.; Ng, M. L.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 989-995
- ISSN:
- 0304-8608
- Subject:
- cytoplasm; hamsters; kidneys; mutants; neutralization; virion; viruses
- Abstract:
- ... Entry processes were compared between dengue-2 (DEN-2) virus and a DEN-2 neutralisation escape mutant virus in baby hamster kidney (BHK) cells. The mutant virus (mu6B2) was resistant to neutralisation by a monoclonal antibody, MAb 6B2. Infection of BHK cells by the wild-type DEN-2 virus resulted in direct penetration of the virions into the cytoplasm whereas the mutant virus entered cells by endoc ...
- DOI:
- 10.1007/s007050050561
- PubMed:
- 10416380
-
http://dx.doi.org/10.1007/s007050050561
- Author:
- Volkova, T. D.; Vorovitch, M. F.; Ivanov, V. T.; Timofeev, A. V.; Volpina, O. M.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 1035-1039
- ISSN:
- 0304-8608
- Subject:
- Tick-borne encephalitis virus; artificial membranes; viruses
- Abstract:
- ... The fusion motif of tick-borne encephalitis virus E protein has been predicted to be located within its conserved region (98–120). Results are presented to demonstrate that non-neutralizing monoclonal antibody which recognizes a synthetic peptide corresponding to residues 98–113 of the E protein sequence can block the fusion of the virus particles with artificial membranes. ...
- DOI:
- 10.1007/s007050050566
- PubMed:
- 10416385
-
http://dx.doi.org/10.1007/s007050050566
- Author:
- Amadori, M.; Archetti, I. L.; Scaccaglia, P.; Modena, D.; Fossati, G.; Lucietto, P.; Mascagni, P.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 905-919
- ISSN:
- 0304-8608
- Subject:
- Foot-and-mouth disease virus; Mycobacterium tuberculosis; antiserum; antiviral properties; cattle; foot-and-mouth disease; guinea pigs; histones; immune response; immune system; immunoblotting; swine; viruses
- Abstract:
- ... Chaperonin 10 of M. tuberculosis conferred partial or total protection against generalized foot-and-mouth disease (FMD) in guinea-pigs challenged with O₁ Lausanne FMD virus. Chaperonin 10-immunized animals mounted an antibody response to the protein, one epitope of which was found in the C-terminal half. A similar recognition pattern was observed in FMD-convalescent guinea-pigs, swine and cattle. ...
- DOI:
- 10.1007/s007050050555
- PubMed:
- 10416374
-
http://dx.doi.org/10.1007/s007050050555
- Author:
- Jendrach, M.; Thiel, V.; Siddell, S.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 921-933
- ISSN:
- 0304-8608
- Subject:
- Murine hepatitis virus; electrophoresis; membrane proteins; messenger RNA; open reading frames; ribosomes; start codon; translation (genetics); viruses
- Abstract:
- ... The unique region of mRNA 5 of murine hepatitis virus contains two open reading frames, ORF 5a and ORF 5b. The downstream ORF 5b encodes the envelope (E) protein, an integral membrane protein of the virus. We have shown previously that the expression of ORF 5b is mediated by the internal entry of ribosomes. In the experiments reported here, we have used the in vitro translation of synthetic mRNAs ...
- DOI:
- 10.1007/s007050050556
- PubMed:
- 10416375
-
http://dx.doi.org/10.1007/s007050050556
- Author:
- Huang, J.; Liu, X.; Levin, D. B.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 935-955
- ISSN:
- 0304-8608
- Subject:
- DNA replication; Lymantria dispar; Malacosoma disstria; Nucleopolyhedrovirus; RNA; genes; insects; luciferase; progeny; transcriptional activation; translation (genetics)
- Abstract:
- ... We have examined Spodoptera littoralis type B nucleopolyhedrovirus (SpliNPV) infections in CLS79, Sf9, and Se1 cells derived from lepidopteran insects of the genus Spodoptera (Family: Noctuidae), Ld652Y cells from Lymantria dispar (Family: Lymantriidae), and Md210 cells from Malacosoma disstria (Family: Lasiocampidae). CLS79, Sf9, and Se1 cells were permissive for SpliNPV infection as these cell l ...
- DOI:
- 10.1007/s007050050557
- PubMed:
- 10416376
-
http://dx.doi.org/10.1007/s007050050557
- Author:
- Fukutomi, T.; Tsunemitsu, H.; Akashi, H.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 997-1006
- ISSN:
- 0304-8608
- Subject:
- Bovine coronavirus; adults; chickens; cows; diarrhea; electron microscopy; enzyme-linked immunosorbent assay; epizootic diseases; erythrocytes; feces; hemagglutination; mice; monoclonal antibodies; neutralization tests; reverse transcriptase polymerase chain reaction; viruses; Japan
- Abstract:
- ... Bovine coronavirus (BCV) was detected by reverse transcriptase-PCR, immune electron microscopy or virus isolation from adult cows at 6 out of 6 outbreaks of epizootic diarrhea in Japan. Six BCVs isolated in feces, intestinal content or tracheal exudate of the cows were analyzed for their antigenic properties by cross virus neutralization (VN) tests. The isolates were divided into two groups, one o ...
- DOI:
- 10.1007/s007050050562
- PubMed:
- 10416381
-
http://dx.doi.org/10.1007/s007050050562
- Author:
- Oleksiewicz, M. B.; Bøtner, A.; Nielsen, J.; Storgaard, T.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 981-987
- ISSN:
- 0304-8608
- Subject:
- Porcine reproductive and respiratory syndrome virus; binding proteins; genome; virulence; virus replication
- Abstract:
- ... We determined the untranslated 5′-leader sequence for three different isolates of porcine reproductive and respiratory syndrome virus (PRRSV): pathogenic European- and American-types, as well as an American-type vaccine strain. 5′-leader from European- and American-type PRRSV differed in length (220 and 190 nt, respectively), and exhibited only approximately 50% nucleotide homology. Nevertheless, ...
- DOI:
- 10.1007/s007050050560
- PubMed:
- 10416379
-
http://dx.doi.org/10.1007/s007050050560
- Author:
- Miyanishi, M.; Kusume, T.; Saito, M.; Tamada, T.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 879-892
- ISSN:
- 0304-8608
- Subject:
- Beet necrotic yellow vein virus; RNA; coat proteins; genes; mixed infection; nucleotide sequences; sequence analysis; viruses; China; France; Japan
- Abstract:
- ... About half of Japanese isolates of beet necrotic yellow vein virus (BNYVV) were found to contain RNA 5 molecules, which were also detected in virus isolates from China and France. Sequence comparisons of RNA 5 (nucleotides 327 to 1171) in 25 isolates showed that there are up to 8% sequence differences, and that RNA 5 variants fall into three groups: group I contains most of the Japanese and Chines ...
- DOI:
- 10.1007/s007050050553
- PubMed:
- 10416372
-
http://dx.doi.org/10.1007/s007050050553
- Author:
- Esumi, M.; Rikihisa, T.; Nishimura, S.; Goto, J.; Mizuno, K.; Zhou, Y.-H.; Shikata, T.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 973-980
- ISSN:
- 0304-8608
- Subject:
- Hepatitis C virus; Pan troglodytes; antiserum; glycoproteins; humoral immunity; intravenous injection; neutralization; peptides; vaccination
- Abstract:
- ... A chimpanzee was immunized with two recombinant envelope glycoproteins E1 and E2 of hepatitis C virus (HCV), strain HCV-N2, and the hypervariable region 1 (HVR1) peptides of a different isolate, HCV-#6, then received an intravenous inoculation of 10 chimpanzee infectious doses of HCV-#6. With high humoral immune response against E1 and E2 but a low response against HVR1, the vaccinee became infect ...
- DOI:
- 10.1007/s007050050559
- PubMed:
- 10416378
-
http://dx.doi.org/10.1007/s007050050559
- Author:
- Jaspars, E. M. J.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 843-863
- ISSN:
- 0304-8608
- Subject:
- Alfalfa mosaic virus; Ilarvirus; RNA; capsid; coat proteins; exoribonucleases; genome; models; virus replication; viruses
- Abstract:
- ... Alfamo- and ilarviruses are characterized by the deficiency of their genomes (three messenger-sense RNAs) to start an infection cycle. The RNAs are in capsids built from a single species of protein of about 24 kD. A few dimers of this coat protein per RNA molecule are sufficient to activate the genome. Since the first description of genome activation [Bol JF, van Vloten-Doting L, Jaspars EMJ (1971 ...
- DOI:
- 10.1007/s007050050551
- PubMed:
- 10416370
-
http://dx.doi.org/10.1007/s007050050551
- Author:
- Yoshimoto, J.; Kakui, M.; Iwasaki, H.; Fujiwara, T.; Sugimoto, H.; Hattori, N.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 865-878
- ISSN:
- 0304-8608
- Subject:
- Influenza A virus; drugs; heat treatment; hemagglutinins; hemolysis; human influenza; pH; viruses
- Abstract:
- ... Stachyflin is a novel compound having H1 and H2 subtype-specific anti-influenza A virus activity. Stachyflin has no inhibition on H3 subtype influenza A or influenza B viruses. The susceptibility of the reassortant viruses between H1 and H3 subtype influenza A viruses to Stachyflin indicated that its target was virus-encoded hemagglutinin (HA). The results of the timing of Stachyflin addition agai ...
- DOI:
- 10.1007/s007050050552
- PubMed:
- 10416371
-
http://dx.doi.org/10.1007/s007050050552
- Author:
- Zhang, Y.; Lartey, R. T.; Hartson, S. D.; Voss, T. C.; Melcher, U.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 957-971
- ISSN:
- 0304-8608
- Subject:
- RNA; Tobacco mosaic virus; Turnip vein-clearing virus; chimerism; complementary DNA; evolution; leaves; plasmids; tobacco; transgenes; turnips
- Abstract:
- ... Turnip vein-clearing virus (TVCV) and tobacco mosaic virus (TMV) represent subgroups of tobamoviruses infecting cruciferous and solanaceous plants, respectively. To identify adaptations that may have been necessary in the evolution of the TVCV subgroup from a TMV-like ancestor, the infection of turnip plants by TMV and by chimeras between TMV and TVCV was explored. TMV accumulated at spatially lim ...
- DOI:
- 10.1007/s007050050558
- PubMed:
- 10416377
-
http://dx.doi.org/10.1007/s007050050558
- Author:
- Koenen, F.; Vanderhallen, H.; Dickinson, N. D.; Knowles, N. J.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 893-903
- ISSN:
- 0304-8608
- Subject:
- genes; nucleotide sequences; phylogeny; rodents; swine; viruses; Belgium; Cyprus; France; Greece; Italy
- Abstract:
- ... The phylogenetic relationships of encephalomyocarditis (EMC) viruses isolated from pigs and rodents in Europe were determined by comparison of nucleotide sequences from two different regions of the virus genome, the VP3/VP1 gene junction (part of the capsid-coding region) and part of the 3D polymerase-coding region. Thirty-five European EMC viruses could be divided into two genetic groups, one whi ...
- DOI:
- 10.1007/s007050050554
- PubMed:
- 10416373
-
http://dx.doi.org/10.1007/s007050050554
- Author:
- Mphahlele, M. J.; Peenze, I.; Steele, A. D.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 1027-1034
- ISSN:
- 0304-8608
- Subject:
- RNA; Rotavirus; children; diarrhea; genes; genotype; South Africa
- Abstract:
- ... Human rotavirus VP4P[14] strains were previously recovered in South Africa [18]. The strains exhibited a long RNA pattern, VP6 subgroup II and VP7 serotype G1. Two of the VP8* genes were cloned and sequenced and demonstrated a high nucleotide homology with the prototype P[14] strains (PAff169 and HAL1166). ...
- DOI:
- 10.1007/s007050050565
- PubMed:
- 10416384
-
http://dx.doi.org/10.1007/s007050050565
- Author:
- Sadanari, H.; Yamada, R.; Tanaka, J.; Murayama, T.; Ohnishi, K.; Matsubara, K.; Fukuda, S.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 1015-1025
- ISSN:
- 0304-8608
- Subject:
- Human herpesvirus 5; adenosine monophosphate; central nervous system; cyclic AMP; enhancer elements; genes; proteins
- Abstract:
- ... The effect of dibutyryl cyclic AMP (dbcAMP) on interaction of human cytomegalovirus (HCMV) with human central nervous system cell lines was examined. Activation of the major immediate-early (IE) promoter (MIEP) of HCMV by dbcAMP was observed in human neuroblastoma IMR-32 cells, but not in glioma 118MGC and astrocytoma U373-MG cells. The 19 bp repeat element in the enhancer of the MIEP was most lik ...
- DOI:
- 10.1007/s007050050564
- PubMed:
- 10416383
-
http://dx.doi.org/10.1007/s007050050564
- Author:
- Seah, E. L.; Gunesekere, I. C.; Marshall, J. A.; Wright, P. J.
- Source:
- Archives of virology 1999 v.144 no.5 pp. 1007-1014
- ISSN:
- 0304-8608
- Subject:
- Norwalk virus; glycosylation; open reading frames; signal peptide; viruses
- Abstract:
- ... The nucleotide sequences of the 3′-terminal open reading frame (ORF3) and 3′ untranslated region (3′UTR) were determined for four Norwalk-like viruses (NLVs) belonging to genogroup 2. Three of the viruses, isolated in 1995 and 1996, were closely related to Mexico virus (92–93% nucleotide identity in ORF3). The fourth virus, isolated in 1984, was unique, showing only 49–58% nucleotide identity with ...
- DOI:
- 10.1007/s007050050563
- PubMed:
- 10416382
-
http://dx.doi.org/10.1007/s007050050563