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You searched for: Journal name BBA - General Subjects Remove constraint Journal name: BBA - General Subjects Publication year rev 7985-2015 Remove constraint Publication year rev: 7985-2015 Source 2015 v.1850 Remove constraint Source: 2015 v.1850
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1. 13C NMR based profiling unveils different α-ketoglutarate pools involved into glutamate and lysine synthesis in the milk yeast Kluyveromyces lactis

Author:
D. Gorietti; E. Zanni; C. Palleschi; M. Delfini; D. Uccelletti; M. Saliola; C. Puccetti; A.P. Sobolev; L. Mannina; A. Miccheli
Source:
BBA - General Subjects 2015 v.1850 pp. 2222-2227
ISSN:
0304-4165
Subject:
Kluyveromyces marxianus var. lactis; alpha-ketoglutaric acid; biosynthesis; genes; glucose; glutamic acid; lysine; metabolic engineering; metabolites; metabolomics; milk synthesis; mitochondria; models; mutants; non-specific serine/threonine protein kinase; nuclear magnetic resonance spectroscopy; pentoses; phosphates; stable isotopes; transaldolase; transketolase; tricarboxylic acid cycle; yeasts
Abstract:
... The construction of efficient cell factories for the production of metabolites requires the rational improvement/engineering of the metabolism of microorganisms. The subject of this paper is directed towards the quantitative understanding of the respiratory/fermentative Kluyveromyces lactis yeast metabolism and its rag8 casein kinase mutant, taken as a model for all rag gene mutations.13C NMR spec ...
DOI:
10.1016/j.bbagen.2015.07.008

2. Access channels to the buried active site control substrate specificity in CYP1A P450 enzymes

Author:
Philippe Urban; Gilles Truan; Denis Pompon
Source:
BBA - General Subjects 2015 v.1850 pp. 696-707
ISSN:
0304-4165
Subject:
active sites; catalytic activity; chimerism; cytochrome P-450; enzymes; mutation; statistical analysis; substrate specificity; surface proteins
Abstract:
... A cytochrome P450 active site is buried within the protein molecule and several channels connect the catalytic cavity to the protein surface. Their role in P450 catalysis is still matter of debate. The aim of this study was to understand the possible relations existing between channels and substrate specificity.Time course studies were carried out with a collection of polycyclic substrates of incr ...
DOI:
10.1016/j.bbagen.2014.12.015

3. Activation of muscarinic receptors in rat parotid acinar cells induces AQP5 trafficking to nuclei and apical plasma membrane

Author:
Gota Cho; Aneta M. Bragiel; Di Wang; Tomasz D. Pieczonka; Mariusz T. Skowronski; Masayuki Shono; Søren Nielsen; Yasuko Ishikawa
Source:
BBA - General Subjects 2015 v.1850 pp. 784-793
ISSN:
0304-4165
Subject:
Western blotting; acinar cells; agonists; atropine; cholinergic receptors; cytoplasm; detergents; microscopy; nuclear membrane; octoxynol; plasma membrane; rats; shrinkage; solubility; sucrose; tissues
Abstract:
... The subcellular distribution of aquaporin-5 (AQP5) in rat parotid acinar cells in response to muscarinic acetylcholine receptor (mAChR) activation remains unclear.Immunoconfocal and immunoelectron microscopy were used to visualize the distribution of AQP5 in parotid acinar cells. Western blotting was used to analyze AQP5 levels in membranes. To clarify the characteristics of membrane domains assoc ...
DOI:
10.1016/j.bbagen.2015.01.009

4. All-atom crystal simulations of DNA and RNA duplexes

Author:
Chunmei Liu; Pawel A. Janowski; David A. Case
Source:
BBA - General Subjects 2015 v.1850 pp. 1059-1071
ISSN:
0304-4165
Subject:
DNA; RNA; crystal structure; hydrogen bonding; models; molecular dynamics
Abstract:
... Molecular dynamics simulations can complement experimental measures of structure and dynamics of biomolecules. The quality of such simulations can be tested by comparisons to models refined against experimental crystallographic data.We report simulations of DNA and RNA duplexes in their crystalline environment. The calculations mimic the conditions for PDB entries 1D23 [d(CGATCGATCG)2] and 1RNA [( ...
DOI:
10.1016/j.bbagen.2014.09.018
PubMed:
25255706
PubMed Central:
PMC4339523

5. Allosteric regulation of the partitioning of glucose-1-phosphate between glycogen and trehalose biosynthesis in Mycobacterium tuberculosis

Author:
Matías D. Asención Diez; Ana M. Demonte; Karl Syson; Diego G. Arias; Andrii Gorelik; Sergio A. Guerrero; Stephen Bornemann; Alberto A. Iglesias
Source:
BBA - General Subjects 2015 v.1850 pp. 13-21
ISSN:
0304-4165
Subject:
Mycobacterium tuberculosis; alpha,alpha-trehalose-phosphate synthase (UDP-forming); biochemical pathways; biosynthesis; fructose 6-phosphate; glucose 1-phosphate; glucose 6-phosphate; glucose-1-phosphate adenylyltransferase; glycogen; glycogen (starch) synthase; humans; pathogens; prokaryotic cells; substrate specificity; trehalose
Abstract:
... Mycobacterium tuberculosis is a pathogenic prokaryote adapted to survive in hostile environments. In this organism and other Gram-positive actinobacteria, the metabolic pathways of glycogen and trehalose are interconnected.In this work we show the production, purification and characterization of recombinant enzymes involved in the partitioning of glucose-1-phosphate between glycogen and trehalose ...
DOI:
10.1016/j.bbagen.2014.09.023
PubMed:
25277548
PubMed Central:
PMC4331664

6. Amyloidogenic lysozymes accumulate in the endoplasmic reticulum accompanied by the augmentation of ER stress signals

Author:
Yoshiki Kamada; Takahiro Kusakabe; Yasushi Sugimoto
Source:
BBA - General Subjects 2015 v.1850 pp. 1107-1119
ISSN:
0304-4165
Subject:
RNA interference; amyloid; amyloidosis; endoplasmic reticulum; gene overexpression; genes; human cell lines; humans; immunocytochemistry; kidneys; liver; lysozyme; mutants; pellets; point mutation; protein aggregates; signal transduction; spleen; unfolded protein response
Abstract:
... Naturally occurring single mutants, I56T, F57I, W64R and D67H of lysozyme in human, have been known to form abnormal protein aggregates (amyloid fibrils) and to accumulate in several organs, including the liver, spleen and kidney, resulting in familial systemic amyloidosis. These human pathogenic lysozyme variants are considered to raise subtle conformational changes compared to the wild type.Here ...
DOI:
10.1016/j.bbagen.2015.01.018

7. Andrographolide ameliorates diabetic retinopathy by inhibiting retinal angiogenesis and inflammation

Author:
Zengyang Yu; Bin Lu; Yuchen Sheng; Lingyu Zhou; Lili Ji; Zhengtao Wang
Source:
BBA - General Subjects 2015 v.1850 pp. 824-831
ISSN:
0304-4165
Subject:
Andrographis paniculata; angiogenesis; blood serum; diabetic retinopathy; enzyme-linked immunosorbent assay; fluorescent antibody technique; gene expression; inflammation; interleukin-1beta; interleukin-6; medicinal plants; messenger RNA; mice; necrosis; protein synthesis; quantitative polymerase chain reaction; signal transduction; staining; streptozotocin; transcription factor NF-kappa B; tumor necrosis factor-alpha; vascular endothelial growth factor receptors; vascular endothelial growth factors
Abstract:
... Andrographolide (Andro) is the main compound distributed in medicinal herb Andrographis paniculata. This study aims to observe the amelioration of Andro on streptozotocin (STZ)-induced diabetic retinopathy (DR) in mice.STZ-induced non-proliferative DR (NPDR) for 2months and proliferative DR (PDR) for 5month in C57BL/6 mice were used in this study, respectively. Retinal vessels were observed by imm ...
DOI:
10.1016/j.bbagen.2015.01.014

8. Andrographolide decreased VEGFD expression in hepatoma cancer cells by inducing ubiquitin/proteasome-mediated cFos protein degradation

Author:
Lili Ji; Zhiyong Zheng; Liang Shi; Yinjie Huang; Bin Lu; Zhengtao Wang
Source:
BBA - General Subjects 2015 v.1850 pp. 750-758
ISSN:
0304-4165
Subject:
antineoplastic activity; enzyme-linked immunosorbent assay; gene expression; genes; hepatoma; human cell lines; immunohistochemistry; luciferase; messenger RNA; mitogen-activated protein kinase; neoplasm cells; proteasome endopeptidase complex; protein degradation; quantitative polymerase chain reaction; staining; tissues; transcription (genetics); ubiquitination; vascular endothelial growth factors
Abstract:
... Andrographolide (Andro) is reported to inhibit hepatoma tumor growth in our previous studies. This study aims to further search the critical signals involved in such Andro-induced inhibition.The anti-tumor effect of Andro was evaluated in vivo. Cancer PathwayFinder RT2 Profiler™ PCR array was used to find the altered genes. Real-time PCR was used to detect the mRNA expression. Protein expression w ...
DOI:
10.1016/j.bbagen.2015.01.005

9. Anti-angiogenic activities of snake venom CRISP isolated from Echis carinatus sochureki

Author:
Shimon Lecht; Rachel A. Chiaverelli; Jonathan Gerstenhaber; Juan J. Calvete; Philip Lazarovici; Nicholas R. Casewell; Robert Harrison; Peter I. Lelkes; Cezary Marcinkiewicz
Source:
BBA - General Subjects 2015 v.1850 pp. 1169-1179
ISSN:
0304-4165
Subject:
Echis carinatus; adhesion; amino acid sequences; angiogenesis; cytoplasm; endothelial cells; gene expression; growth factors; humans; messenger RNA; mitogen-activated protein kinase; quails; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; snakes; therapeutics; venoms
Abstract:
... Cysteine-rich secretory protein (CRISP) is present in majority of vertebrate including human. The physiological role of this protein is not characterized. We report that a CRISP isolated from Echis carinatus sochureki venom (ES-CRISP) inhibits angiogenesis.The anti-angiogenic activity of purified ES-CRISP from snake venom was investigated in vitro using endothelial cells assays such as proliferati ...
DOI:
10.1016/j.bbagen.2015.02.002

10. Apocrine secretion: New insights into an old phenomenon

Author:
Robert Farkaš
Source:
BBA - General Subjects 2015 v.1850 pp. 1740-1750
ISSN:
0304-4165
Subject:
DNA; Drosophila; bronchi; cytoskeleton; endoplasmic reticulum; epididymis; exocytosis; genetic techniques and protocols; intestinal mucosa; mitochondria; nuclear proteins; proteomics; salivary glands; secretion
Abstract:
... While apocrine secretion was among the earliest secretory mechanisms to be identified, its underlying basis remains poorly understood.This review reappraises our understanding of apocrine secretion using insights about apocrine secretion from the salivary glands of Drosophila, in which molecular genetic analyses have provided a glimmer of hope for elucidating the mechanistic aspects of this fundam ...
DOI:
10.1016/j.bbagen.2015.05.003

11. Bacteriorhodopsin: Would the real structural intermediates please stand up?

Author:
Cecilia Wickstrand; Robert Dods; Antoine Royant; Richard Neutze
Source:
BBA - General Subjects 2015 v.1850 pp. 536-553
ISSN:
0304-4165
Subject:
X-radiation; X-ray diffraction; annealing; aspartic acid; biophysics; crystallization; crystals; databases; mutagenesis; nuclear magnetic resonance spectroscopy; pH; proton pump; schiff bases; trees
Abstract:
... Bacteriorhodopsin (bR) is the simplest known light driven proton pump and has been heavily studied using structural methods: eighty four X-ray diffraction, six electron diffraction and three NMR structures of bR are deposited within the protein data bank. Twenty one X-ray structures report light induced structural changes and changes induced by mutation, changes in pH, thermal annealing or X-ray i ...
DOI:
10.1016/j.bbagen.2014.05.021

12. Basal Flt1 tyrosine kinase activity is a positive regulator of endothelial survival and vascularization during zebrafish embryogenesis

Author:
Shang Li; Xue Lin Zhou; Yuan Ye Dang; Yiu Wa Kwan; Shun Wan Chan; George Pak Heng Leung; Simon Ming-Yuen Lee; Maggie Pui Man Hoi
Source:
BBA - General Subjects 2015 v.1850 pp. 373-384
ISSN:
0304-4165
Subject:
Danio rerio; adenosine triphosphate; angiogenesis; blood vessels; cell viability; cytotoxicity; embryogenesis; endothelial cells; enzyme inhibition; genes; in vitro studies; molecular models; phosphatidylinositol 3-kinase; phosphorylation; precipitin tests; protein synthesis; reverse transcriptase polymerase chain reaction; small interfering RNA; tyrosine; vascular endothelial growth factor receptor-1; vascular endothelial growth factors
Abstract:
... The role of Kdr (VEGFR-2/Flk-1) in vascular formation has been well described, but the role of Flt1 (VEGFR-1) is not well studied and is generally considered as a decoy receptor for trapping VEGF.The effects of VEGFR1/2 kinase inhibitor (VRI) and calycosin on Flt1 tyrosine kinase (TK) activity were evaluated by molecular docking, enzymatic inhibition assay, protein co-immunoprecipitation and siRNA ...
DOI:
10.1016/j.bbagen.2014.10.023

13. Beyond water homeostasis: Diverse functional roles of mammalian aquaporins

Author:
Philip Kitchen; Rebecca E. Day; Mootaz M. Salman; Matthew T. Conner; Roslyn M. Bill; Alex C. Conner
Source:
BBA - General Subjects 2015 v.1850 pp. 2410-2421
ISSN:
0304-4165
Subject:
aquaporins; carbon dioxide; cations; cell adhesion; cell communication; cultured cells; drugs; homeostasis; humans; mutation; solutes; transporters
Abstract:
... Aquaporin (AQP) water channels are best known as passive transporters of water that are vital for water homeostasis.AQP knockout studies in whole animals and cultured cells, along with naturally occurring human mutations suggest that the transport of neutral solutes through AQPs has important physiological roles. Emerging biophysical evidence suggests that AQPs may also facilitate gas (CO2) and ca ...
DOI:
10.1016/j.bbagen.2015.08.023

14. Biosynthesis of cyclosporins and other natural peptidyl prolyl cis/trans isomerase inhibitors

Author:
Alfons Lawen
Source:
BBA - General Subjects 2015 v.1850 pp. 2111-2120
ISSN:
0304-4165
Subject:
Protozoa; antiviral agents; autoimmune diseases; biosynthesis; catalysts; cyclophilins; cyclosporins; engineering; immunosuppression; ligases; neoplasms; peptidylprolyl isomerase; polyketide synthases; synthesis
Abstract:
... Peptidyl-prolyl-cis/trans-isomerases (PPIases) are ubiquitously expressed and have been implicated in a wide range of biological functions. Their inhibition is beneficial in immunosuppression, cancer treatment, treatment of autoimmune diseases, protozoan and viral infections.Three classes of PPIases are known, each class having their own specific inhibitors. This review will cover the present know ...
DOI:
10.1016/j.bbagen.2014.12.009

15. C1-inhibitor polymers activate the FXII-dependent kallikrein–kinin system: Implication for a role in hereditary angioedema

Author:
Daniel Elenius Madsen; Johannes Jakobsen Sidelmann; Daniel Biltoft; Jørgen Gram; Soren Hansen
Source:
BBA - General Subjects 2015 v.1850 pp. 1336-1342
ISSN:
0304-4165
Subject:
denaturation; dose response; edema; enzyme-linked immunosorbent assay; gel chromatography; genes; heat; in vitro studies; mutation; pathophysiology; patients; polymerization; polymers; proteinase inhibitors; serine proteinases
Abstract:
... The FXII-dependent kallikrein–kinin system (KKS) is tightly regulated by the serine protease inhibitor (serpin) C1-inhibitor (C1-inh). When regulation of the FXII-dependent KKS fails, which is the case in hereditary angioedema (HAE), patients consequently experience invalidating edema attacks. HAE is caused by mutations in the C1-inh encoding gene, and we recently demonstrated that some mutations ...
DOI:
10.1016/j.bbagen.2015.03.005

16. Changes to both cardiac metabolism and performance accompany acute reductions in functional capillary supply

Author:
David Hauton; James Winter; Abdullah A. Al-Shammari; Eamonn A. Gaffney; Rhys D. Evans; Stuart Egginton
Source:
BBA - General Subjects 2015 v.1850 pp. 681-690
ISSN:
0304-4165
Subject:
arterioles; cardiac output; diabetes; dose response; fatty acids; glucose; heart; hypoxia; metabolism; metabolites; oxidation; oxygen; oxygen consumption; palmitates; polystyrenes
Abstract:
... The relative importance of arteriole supply or ability to switch between substrates to preserve cardiac performance is currently unclear, but may be critically important in conditions such as diabetes.Metabolism of substrates was measured before and after infusion of polystyrene microspheres in the perfused working heart to mimic random capillary loss due to microvascular disease. The effect of ac ...
DOI:
10.1016/j.bbagen.2014.12.014

17. Characterisation of chlorate reduction in the haloarchaeon Haloferax mediterranei

Author:
Rosa María Martínez-Espinosa; David J. Richardson; María José Bonete
Source:
BBA - General Subjects 2015 v.1850 pp. 587-594
ISSN:
0304-4165
Subject:
Haloferax mediterranei; active sites; anaerobic conditions; cell membranes; culture media; denitrification; enzyme activity; genes; ions; nitrates; perchlorates; wastewater treatment
Abstract:
... Haloferax mediterranei is a denitrifying haloarchaeon using nitrate as a respiratory electron acceptor under anaerobic conditions in a reaction catalysed by pNarGH. Other ions such as bromate, perchlorate and chlorate can also be reduced.Hfx. mediterranei cells were grown anaerobically with nitrate as electron acceptor and chlorate reductase activity measured in whole cells and purified nitrate re ...
DOI:
10.1016/j.bbagen.2014.12.011

18. Chemical and thermal influence of the [4Fe–4S]2+ cluster of A/G-specific adenine glycosylase from Corynebacterium pseudotuberculosis

Author:
Raphael J. Eberle; Monika A. Coronado; Icaro P. Caruso; Débora O. Lopes; Anderson Miyoshi; Vasco Azevedo; Raghuvir K. Arni
Source:
BBA - General Subjects 2015 v.1850 pp. 393-400
ISSN:
0304-4165
Subject:
Corynebacterium pseudotuberculosis; DNA; EDTA (chelating agent); Escherichia coli; Gram-positive bacteria; adenine; caseous lymphadenitis; copper sulfate; death; differential scanning calorimetry; fluorescence emission spectroscopy; livestock; mutation; pathogens; thermal stability
Abstract:
... The gram-positive bacteria Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis in livestock significantly reduces productivity and often causes death. The adenine/guanine-specific DNA glycosylase (MutY) prevents mutations in the DNA of the pathogen and a unique feature of the MutY protein family is the [4Fe–4S]2+ cluster that interlinks two protein subdomains. MutY fro ...
DOI:
10.1016/j.bbagen.2014.11.014

19. Chip physically interacts with Notch and their stoichiometry is critical for Notch function in wing development and cell proliferation in Drosophila

Author:
Nalani Sachan; Abhinava K. Mishra; Mousumi Mutsuddi; Ashim Mukherjee
Source:
BBA - General Subjects 2015 v.1850 pp. 802-812
ISSN:
0304-4165
Subject:
Drosophila; cell nucleus; cell proliferation; clones; gain-of-function mutation; larvae; loss-of-function mutation; mutants; quantitative polymerase chain reaction; stoichiometry; titration; transcription (genetics); two hybrid system techniques
Abstract:
... Notch signaling plays a fundamental role both in metazoan cell fate determination and in the establishment of distinct developmental cell lineages. In a yeast two-hybrid screen, we identified Chip as a binding partner of Notch. Thus, we investigated the functional significance of Notch and Chip interactions.Co-immunoprecipitation and GST pull-down experiments confirmed the physical interaction bet ...
DOI:
10.1016/j.bbagen.2014.12.026

20. Cidec promotes the differentiation of human adipocytes by degradation of AMPKα through ubiquitin-proteasome pathway

Author:
Yuqiao Xu; Yu Gu; Gang Liu; Feng Zhang; Jie Li; Fang Liu; Zhiwen Zhang; Jing Ye; Qing Li
Source:
BBA - General Subjects 2015 v.1850 pp. 2552-2562
ISSN:
0304-4165
Subject:
adipocytes; adipogenesis; droplets; humans; lipids; obesity; obesity-related diseases; precipitin tests; proteins; two hybrid system techniques
Abstract:
... We previously showed that Cidec was localized on the surface of lipid droplets and could promote the differentiation of human adipocytes, but the molecular mechanism was still unknown.In this study, we first sought to identify proteins that interact with Cidec using yeast two-hybrid system. The results revealed that Cidec could directly interact with AMPKα1 subunit. We further showed that AMPKα le ...
DOI:
10.1016/j.bbagen.2015.09.008