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- Schäfer, Martina, et al. Show all 6 Authors
- BBA - General Subjects 2015 v.1850 pp. 647-656
- Gibbs free energy; calorimetry; decision making; enthalpy; ligands; proteins; solubility; titration
- ... Detailed characterization of the thermodynamic signature of weak binding fragments to proteins is essential to support the decision making process which fragments to take further for the hit-to-lead optimization.Isothermal titration calorimetry (ITC) is the method of choice to record thermodynamic data, however, weak binding ligands such as fragments require the development of meaningful and relia ...