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You searched for: Journal name Biochemical and biophysical research communications Remove constraint Journal name: Biochemical and biophysical research communications Publication year rev 7980-2020 Remove constraint Publication year rev: 7980-2020 Source 2020 v.521 no.3 Remove constraint Source: 2020 v.521 no.3
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1. Artificial small RNA-mediated growth inhibition in Escherichia coli and Salmonella enterica serovar Typhimurium

Author:
Mitsuoki Kawano; Shota Morohashi; Kohei Oda; Masataka Ishikawa; Shouta Fujita; Mineki Saito
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 577-583
ISSN:
0006-291X
Subject:
Escherichia coli; RNA; Salmonella Typhimurium; agar; genetic vectors; growth retardation; guanine; liquids; oligonucleotides; research
Abstract:
... We developed a synthetic RNA approach to identify growth inhibition sequences by cloning random 24-nucleotide (nt) sequences into an arabinose-inducible expression vector. This vector expressed a small RNA (sRNA) of ∼140 nt containing a 24 nt random sequence insert. After transforming Escherichia coli with the vector, 10 out of 954 transformants showed strong growth defect phenotypes and two clone ...
DOI:
10.1016/j.bbrc.2019.10.060

2. Bmi1 counteracts hematopoietic stem cell aging by repressing target genes and enforcing the stem cell gene signature

Author:
Eriko Nitta; Naoki Itokawa; Shogo Yabata; Shuhei Koide; Li-Bo Hou; Motohiko Oshima; Kazumasa Aoyama; Atsunori Saraya; Atsushi Iwama
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 612-619
ISSN:
0006-291X
Subject:
cell transplantation; chromatin; hematopoietic stem cells; histones; nucleotide sequences; research; sequence analysis; transcription (genetics)
Abstract:
... Polycomb-group proteins are critical regulators of stem cells. We previously demonstrated that Bmi1, a component of polycomb repressive complex 1, defines the regenerative capacity of hematopoietic stem cells (HSCs). Here, we attempted to ameliorate the age-related decline in HSC function by modulating Bmi1 expression. The forced expression of Bmi1 did not attenuate myeloid-biased differentiation ...
DOI:
10.1016/j.bbrc.2019.10.153

3. Inhibition of microtubule assembly competent tubulin synthesis leads to accumulation of phosphorylated tau in neuronal cell bodies

Author:
Hitomi Fujiwara; Shoji Watanabe; Minori Iwata; Shouyou Ueda; Mika Nobuhara; Satoko Wada-Kakuda; Hiroaki Misonou; Tomohiro Miyasaka
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 779-785
ISSN:
0006-291X
Subject:
biosynthesis; homeostasis; mice; microtubules; neurons; neurotoxicity; pathogenesis; research; tubulin
Abstract:
... Neurofibrillary tangles, a pathological hallmark of Alzheimer’s disease (AD), are somatodendritic filamentous inclusions composed of hyperphosphorylated tau. Microtubule loss is also a common feature of affected neurons in AD. However, whether and how the disruptions of microtubules and the microtubule-associated proteins occur in the pathogenesis of AD remain unclear. Recent evidence indicates th ...
DOI:
10.1016/j.bbrc.2019.10.191

4. O-GlcNAcylation-mediated degradation of FBXL2 stabilizes FOXM1 to induce cancer progression

Author:
Yasuhiro Ueda; Kazumasa Moriwaki; Toshihisa Takeuchi; Kazuhide Higuchi; Michio Asahi
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 632-638
ISSN:
0006-291X
Subject:
cell proliferation; humans; neoplasm cells; neoplasm progression; research; stomach neoplasms; transcription factors; ubiquitin-protein ligase; ubiquitination
Abstract:
... O-GlcNAcylation is a dynamic and reversible post-translational modification of cytonuclear molecules that regulates cellular signaling. Elevated O-GlcNAcylation is a general property of cancer and plays a critical role in cancer progression. We previously showed that the expression of FOXM1, a critical oncogenic transcription factor widely overexpressed in solid tumors, was elevated in MKN45 cells ...
DOI:
10.1016/j.bbrc.2019.10.164

5. Phagomimetic action of antibiotics: Revisited. How do antibiotics know where to go?

Author:
John M.C. Gutteridge; Gregory J. Quinlan; Peter Kovacic
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 721-724
ISSN:
0006-291X
Subject:
active sites; cell growth; chemotaxis; electron transfer; neoplasms; pathogens; phagocytosis; research; virulence
Abstract:
... Phagocytic cells know exactly where an infection is by following chemotactic signals. The phagocytosis of bacteria results in a ‘respiratory burst’ in which superoxide radicals are released. We have previously compared the release of reactive oxygen species (ROS) by antibiotics, during electron transfer reactions, to this event. Antibiotics in their normal bacterial environment, and ROS, are both ...
DOI:
10.1016/j.bbrc.2019.10.152

6. Progranulin protects against cerebral ischemia-reperfusion (I/R) injury by inhibiting necroptosis and oxidative stress

Author:
Xiaogang Li; Shaoli Cheng; Hao Hu; Xiaotian Zhang; Jiehua Xu; Rui Wang; Pengbo Zhang
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 569-576
ISSN:
0006-291X
Subject:
brain damage; caspase-3; caspase-8; glycoproteins; infarction; ischemia; lipid peroxidation; malondialdehyde; mortality; necroptosis; oxidative stress; reactive oxygen species; research; stroke; superoxide dismutase; surgery
Abstract:
... Ischemic stroke is a leading cause of mortality and disability worldwide. Nevertheless, its molecular mechanisms have not yet been adequately illustrated. Progranulin (PGRN) is a secreted glycoprotein with pleiotropic functions. In the present study, we found that PGRN expression was markedly reduced in mice after stroke onset through middle cerebral artery occlusion (MCAO). We also showed that ne ...
DOI:
10.1016/j.bbrc.2019.09.111

7. Protection of DAergic neurons mediates treadmill running attenuated olfactory deficits and olfactory neurogenesis promotion in depression model

Author:
Yuan Tian; Jianguo Dong; Dongbo Shi
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 725-731
ISSN:
0006-291X
Subject:
antidepressants; emotions; models; neurogenesis; neurons; research
Abstract:
... In this study, we aimed to test the effects of treadmill running on depression induced olfactory functions and OB neurogenesis in depression model. Depression model was created with chronic unpredictable mild stress (CUMS) and treadmill running was performed as the antidepressant treatment. Behavioral results showed that treadmill running not only attenuated the depression mood but also improved t ...
DOI:
10.1016/j.bbrc.2019.10.158

8. Roles of CytR, an anti-activator of cyclic-AMP receptor protein (CRP) on flagellar expression and virulence in uropathogenic Escherichia coli

Author:
Hidetada Hirakawa; Ayako Takita; Mao Kato; Hanano Mizumoto; Haruyoshi Tomita
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 555-561
ISSN:
0006-291X
Subject:
bacteria; bacterial motility; bladder; cyclic AMP; epithelium; infectious diseases; mutants; pathogens; research; urinary tract diseases; uropathogenic Escherichia coli; virulence
Abstract:
... Uropathogenic Escherichia coli (UPEC) is a major pathogen that causes urinary tract infection (UTI), a common bacterial infectious disease. This bacterium invades the urinary tract cells, where it aggregates, and subsequently forms multicellular colonies termed intracellular bacterial communities (IBCs). The motility of the bacteria plays a key role in the mechanism of virulence in the host bladde ...
DOI:
10.1016/j.bbrc.2019.10.165

9. Roles of the indole ring of Trp396 covalently bound with the imidazole ring of His398 coordinated to type I copper in bilirubin oxidase

Author:
Kunishige Kataoka; Takahiro Ito; Yoko Okuda; Yoko Sakai; Satoshi Yamashita; Takeshi Sakurai
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 620-624
ISSN:
0006-291X
Subject:
bilirubin oxidase; chemical bonding; copper; electrochemistry; electron transfer; imidazole; indoles; magnetism; moieties; redox potential; research
Abstract:
... Bilirubin oxidase has a post-translationally formed covalent-bond between the imidazole ring of His398 coordinated to type I copper and the indole ring of Trp396 located in the outer-coordination sphere. We performed point mutations at Trp396 with Ala, Thr, Phe, and Tyr with the aim of elucidating the role of the imidazole-indole moiety found only in bilirubin oxidase. The result showed shifts in ...
DOI:
10.1016/j.bbrc.2019.10.159

10. Tadalafil alleviates preeclampsia and fetal growth restriction in RUPP model of preeclampsia in mice

Author:
Akiyo Sekimoto; Kayo Tanaka; Yamato Hashizume; Emiko Sato; Hiroshi Sato; Tomoaki Ikeda; Nobuyuki Takahashi
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 769-774
ISSN:
0006-291X
Subject:
animal models; clinical trials; fetal development; hypertension; mice; phenotype; placenta; pre-eclampsia; pregnancy; research
Abstract:
... – Tadalafil, a long-acting phosphodiesterase 5 (PDE5) inhibitor, alleviates preeclampsia (PE), and decreases the fetal and infant deaths associated with fetal growth restriction (FGR) in phase II clinical trial. Recently, we demonstrated that tadalafil alleviates FGR and hypertension in the dams with PE induced by l-NAME.–The aim of present study was to clarify the effect of tadalafil in another m ...
DOI:
10.1016/j.bbrc.2019.10.186

11. The tetramerization domain of the tree shrew p53 protein displays unique thermostability despite sharing high sequence identity with the human p53 protein

Author:
Natsumi Nakagawa; Shuya Sakaguchi; Takao Nomura; Rui Kamada; James G. Omichinski; Kazuyasu Sakaguchi
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 681-686
ISSN:
0006-291X
Subject:
DNA damage; Scandentia; acetaldehyde; alcohols; diet; glutamine; humans; metabolites; methionine; mutagenesis; mutagens; research; sequence analysis; thermal stability; transcription (genetics)
Abstract:
... The p53 protein plays a number of roles in protecting organisms from different genotoxic stresses and this includes DNA damage induced by acetaldehyde, a metabolite of alcohol. Since the common tree shrew ingests high levels of alcohol as part of its normal diet, this suggests that its p53 protein may possess unique properties. Using a combination of biophysical and modeling studies, we demonstrat ...
DOI:
10.1016/j.bbrc.2019.10.130

12. Transcription factor MafB is a marker of tumor-associated macrophages in both mouse and humans

Author:
Manoj Kumar Yadav; Yuri Inoue; Aya Nakane-Otani; Yuki Tsunakawa; Hyojung Jeon; Omar Samir; Akari Teramoto; Kaushalya Kulathunga; Manabu Kusakabe; Megumi Nakamura; Takashi Kudo; Satoru Takahashi; Michito Hamada
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 590-595
ISSN:
0006-291X
Subject:
angiogenesis; antibodies; cell proliferation; fluorescence; green fluorescent protein; heterozygosity; humans; immunosuppression; interleukin-10; lung neoplasms; macrophages; mice; neoplasm cells; research; reverse transcriptase polymerase chain reaction; transcription factors
Abstract:
... The transcription factor MafB is specifically expressed in macrophages. We have recently demonstrated that MafB is expressed in anti-inflammatory alternatively activated M2 macrophages in vitro. Tumor-associated macrophages (TAMs) are a subset of M2 type macrophages that can promote immunosuppressive activity, induce angiogenesis, and promote tumor cell proliferation. To examine whether MafB expre ...
DOI:
10.1016/j.bbrc.2019.10.125

13. Unjamming and collective migration in MCF10A breast cancer cell lines

Author:
Jae Hun Kim; Adrian F. Pegoraro; Amit Das; Stephan A. Koehler; Sylvia Ann Ujwary; Bo Lan; Jennifer A. Mitchel; Lior Atia; Shijie He; Karin Wang; Dapeng Bi; Muhammad H. Zaman; Jin-Ah Park; James P. Butler; Kyu Ha Lee; Jacqueline R. Starr; Jeffrey J. Fredberg
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 706-715
ISSN:
0006-291X
Subject:
breast neoplasms; carcinoma; cell lines; epithelium; geometry; migratory behavior; neoplasm cells; research
Abstract:
... Each cell comprising an intact, healthy, confluent epithelial layer ordinarily remains sedentary, firmly adherent to and caged by its neighbors, and thus defines an elemental constituent of a solid-like cellular collective [1,2]. After malignant transformation, however, the cellular collective can become fluid-like and migratory, as evidenced by collective motions that arise in characteristic swir ...
DOI:
10.1016/j.bbrc.2019.10.188
PubMed:
31699371
PubMed Central:
PMC6937379

14. A novel FoxM1-PSMB4 axis contributes to proliferation and progression of cervical cancer

Author:
Dong-mei Zhou; Jun Liu; Fang Liu; Guang-wei Luo; Hai-tong Li; Rui Zhang; Bi-liang Chen; Wei Hua
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 746-752
ISSN:
0006-291X
Subject:
cell division; cell growth; cell proliferation; genes; neoplasm cells; promoter regions; proteasome endopeptidase complex; research; uterine cervical neoplasms
Abstract:
... The abnormally high activity of the proteasome system is closely related to the occurrence and development of various tumors. PSMB4 is a non-catalytic subunit for the proteasome assembly. Although the reports from genetic screening have demonstrated it’s a driver gene for cell growth in several types of solid tumor, its expression pattern and regulatory mechanisms in malignant diseases are still e ...
DOI:
10.1016/j.bbrc.2019.10.183

15. Autophagic flux defect in diabetic kidney disease results in megamitochondria formation in podocytes

Author:
Chang-Yun Woo; Ranjan Kc; Mina Kim; Hyoun Sik Kim; Ji Yeon Baek; Eun Hee Koh
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 660-667
ISSN:
0006-291X
Subject:
albuminuria; animals; autophagosomes; autophagy; diabetic nephropathy; glucose; kidneys; lysosomes; mitochondria; pathogenesis; research
Abstract:
... Podocyte injury is an important factor in the pathogenesis of diabetic nephropathy. Podocytes are characterized by large numbers of mitochondria. However, mitochondrial dysfunction as it relates to kidney pathology remains poorly understood. The present study found that podocyte mitochondria in different animal models of diabetes mellitus became elongated with the development of albuminuria, sugge ...
DOI:
10.1016/j.bbrc.2019.10.132

16. Crystal structure of a hypothetical T2SS effector Lpg0189 from Legionella pneumophila reveals a novel protein fold

Author:
Xiaofang Chen; Shan Liu; Sha Jiang; Xuecheng Zhang; Nannan Zhang; Jinming Ma; Honghua Ge
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 799-805
ISSN:
0006-291X
Subject:
Legionella pneumophila; bioinformatics; crystal structure; research; secretion
Abstract:
... Lpg0189 is a type II secretion system-dependent extracellular protein with unknown function from Legionella pneumophila. Herein, we determined the crystal structure of Lpg0189 at 1.98 Å resolution by using single-wavelength anomalous diffraction (SAD). Lpg0189 folds into a novel chair-shaped architecture, with two sheets roughly perpendicular to each other. Bioinformatics analysis suggests Lpg0189 ...
DOI:
10.1016/j.bbrc.2019.10.195

17. DNA-PKcs is activated under nutrient starvation and activates Akt, MST1, FoxO3a, and NDR1

Author:
Soichiro Shiga; Yasuhiko Murata; Takuma Hashimoto; Yusuke Urushihara; Yohei Fujishima; Kanna Kudo; Yaoki Sonohara; Miku Kurusu; Kazuya Takeda; Keiichi Jingu; Yoshio Hosoi
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 668-673
ISSN:
0006-291X
Subject:
DNA; glioblastoma; humans; hypoxia; phosphorylation; radiation resistance; research; signal transduction; starvation
Abstract:
... Presence of unperfused regions containing cells under hypoxia and nutrient starvation; contributes to radioresistance in solid human tumors. We have previously reported that cultured cells; under nutrient starvation show resistance to ionizing radiation compare with cells under normal; condition, and that nutrient starvation increases ATM activity, which causes cellular resistance to; ionizing rad ...
DOI:
10.1016/j.bbrc.2019.10.133

18. Decreased amount of vimentin N-terminal truncated proteolytic products in parkin-mutant skin fibroblasts

Author:
Rosa Anna Siciliano; Maria Fiorella Mazzeo; Anna Ferretta; Consiglia Pacelli; Antonio Rosato; Francesco Papa; Salvatore Scacco; Sergio Papa; Tiziana Cocco; Rosa Lippolis
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 693-698
ISSN:
0006-291X
Subject:
Parkinson disease; fibroblasts; pathogenesis; proteolysis; proteomics; research; vimentin
Abstract:
... Vimentin, a member of cytoskeleton intermediate filaments proteins, plays a critical role in cell structure and dynamics.The present proteomic study reveals reduced amount of six different lengths, N-terminal truncated proteolytic products of vimentin, in the primary skin fibroblasts from two unrelated PD patients, as compared to control fibroblasts. The decreased amount of N-terminal truncated fo ...
DOI:
10.1016/j.bbrc.2019.10.154

19. Ebola virus replication is regulated by the phosphorylation of viral protein VP35

Author:
Lin Zhu; Ting Gao; Weihong Yang; Yaoning Liu; Xuan Liu; Yong Hu; Yanwen Jin; Ping Li; Ke Xu; Gang Zou; Lei Zhao; Ruiyuan Cao; Wu Zhong; Xianzhu Xia; Cheng Cao
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 687-692
ISSN:
0006-291X
Subject:
Ebolavirus; RNA-directed RNA polymerase; genomics; humans; immunoblotting; mutants; pathogens; phosphorylation; research; serine; threonine; virus replication
Abstract:
... Ebola virus (EBOV) is a zoonotic pathogen, the infection often results in severe, potentially fatal, systematic disease in human and nonhuman primates. VP35, an essential viral RNA-dependent RNA polymerase cofactor, is indispensable for Ebola viral replication and host innate immune escape. In this study, VP35 was demonstrated to be phosphorylated at Serine/Threonine by immunoblotting, and the maj ...
DOI:
10.1016/j.bbrc.2019.10.147

20. Effect of potassium ions at the different concentration on the interaction between AmB and the lipid monolayer containing cholesterol or ergosterol

Author:
Juan Wang; Yahong Ma; Suxia Hou
Source:
Biochemical and biophysical research communications 2020 v.521 no.3 pp. 699-705
ISSN:
0006-291X
Subject:
antifungal agents; antifungal properties; biofilm; cholesterol; ergosterol; fungi; mammals; metal ions; modulus of elasticity; nephrotoxicity; potassium; research
Abstract:
... AmB is an antifungal drug of polyene. Although it is prone to nephrotoxicity, it is still the gold standard in the clinical treatment of fungal infection. Sterol plays a decisive role in the drug activity of AmB. The antifungal activity of AmB depends on ergosterol in fungal membranes, and its toxicity is related to cholesterol in mammalian membranes. At the same time, AmB interacts with biofilms, ...
DOI:
10.1016/j.bbrc.2019.10.166