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You searched for: Journal name Biochemical and biophysical research communications Remove constraint Journal name: Biochemical and biophysical research communications Publication year rev 7985-2015 Remove constraint Publication year rev: 7985-2015 Source 2015 v.461 Remove constraint Source: 2015 v.461
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1. Acid–base balance and changes in haemolymph properties of the South African rock lobsters, Jasus lalandii, a palinurid decapod, during chronic hypercapnia

Author:
Jarred L. Knapp; Christopher R. Bridges; Janina Krohn; Louwrens C. Hoffman; Lutz Auerswald
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 475-480
ISSN:
0006-291X
Subject:
Jasus; acid-base balance; bicarbonates; binding properties; chronic exposure; hemocyanin; hemolymph; hypercapnia; juveniles; lobsters; oxygen
Abstract:
... Few studies exist reporting on long-term exposure of crustaceans to hypercapnia. We exposed juvenile South African rock lobsters, Jasus lalandii, to hypercapnic conditions of pH 7.3 for 28 weeks and subsequently analysed changes in the extracellular fluid (haemolymph). Results revealed, for the first time, adjustments in the haemolymph of a palinurid crustacean during chronic hypercapnic exposure: ...
DOI:
10.1016/j.bbrc.2015.04.025

2. Arsenic trioxide synergistically potentiates the cytotoxic effect of fludarabine in chronic lymphocytic leukemia cells by further inactivating the Akt and ERK signaling pathways

Author:
Carol Lozano-Santos; Irene Amigo-Jiménez; Sara Nova-Gurumeta; Nuria Pérez-Sanz; Angeles García-Pardo; José A. García-Marco
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 243-248
ISSN:
0006-291X
Subject:
arsenic; cytotoxicity; drugs; lymphocytic leukemia; mitogen-activated protein kinase; neoplasm cells; patients; signal transduction; stromal cells; synergism
Abstract:
... CLL remains an incurable disease, making it crucial to continue searching for new therapies efficient in all CLL cases. We have studied the effect of combining arsenic trioxide (ATO) with fludarabine, a frontline drug in CLL. We have found a synergistic interaction between 1 μM ATO and 5 μM fludarabine that significantly enhanced the cytotoxic effect of the individual drugs. Importantly, ATO sensi ...
DOI:
10.1016/j.bbrc.2015.04.007

3. Characterization of monoclonal antibodies against porcine pulmonary alveolar macrophages of gnotobiotic miniature swine

Author:
Jeong Ho Hwang; Sang Eun Kim; Sung Han Jung; Young Kyu Kim; Yoon Berm Kim; Hoon Taek Lee
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 427-434
ISSN:
0006-291X
Subject:
antigens; granulocytes; immunoglobulin G; immunoglobulin M; integrins; macrophages; mice; miniature swine; monoclonal antibodies; monocytes; ontogeny; polyacrylamide gel electrophoresis; swine; thimet oligopeptidase
Abstract:
... There is no sufficient porcine specific antibody available to investigate the ontogeny and development of porcine pulmonary alveolar macrophage (PAM). Therefore, several mouse anti-porcine macrophage mAbs have been produced and characterized in this study. First, the monoclonal antibody PM18-7, an IgG1, kappa isotype, bound to 91% of PAM, 6% of monocytes, and 2% of granulocytes indicating PM18-7 w ...
DOI:
10.1016/j.bbrc.2015.04.056

4. Cloning and characterization of HbMT2a, a metallothionein gene from Hevea brasiliensis Muell. Arg differently responds to abiotic stress and heavy metals

Author:
Yan Li; Yue Yi Chen; Shu Guang Yang; Wei Min Tian
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 95-101
ISSN:
0006-291X
Subject:
Escherichia coli; Hevea brasiliensis; abiotic stress; abscisic acid; amino acids; clones; complementary DNA; copper; gene expression regulation; genes; heavy metals; homeostasis; hydrogen peroxide; leaves; metal ions; metallothionein; molecular weight; open reading frames; polyethylene glycol; toxicity; zinc
Abstract:
... Metallothioneins (MTs) are of low molecular mass, cysteine-rich proteins. They play an important role in the detoxification of heavy metals and homeostasis of intracellular metal ions, and protecting against intracellular oxidative damages. In this study a full-length cDNA of type 2 plant metallothioneins, HbMT2a, was isolated from 25 mM Polyethyleneglycol (PEG) stressed leaves of Hevea brasiliens ...
DOI:
10.1016/j.bbrc.2015.03.175

5. Colocalization of insulin and glucagon in insulinoma cells and developing pancreatic endocrine cells

Author:
Zai Wang; Jia You; Shiqing Xu; Zhan Hua; Wenjian Zhang; Tingting Deng; Ni Fang; Qing Fang; Honglin Liu; Liang Peng; Peigang Wang; Jinning Lou
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 598-604
ISSN:
0006-291X
Subject:
glucagon; glucagon-like peptide 1; glucose; humans; insulin; rats
Abstract:
... A significant portion of human and rat insulinomas coexpress multiple hormones. This character termed as multihormonality is also observed in some early pancreatic endocrine cells which coexpress insulin and glucagon, suggesting an incomplete differentiation status of both cells. Here we demonstrate that insulinoma cells INS-1 and INS-1-derived single cell clone INS-1-15 coexpressed insulin and gl ...
DOI:
10.1016/j.bbrc.2015.04.072

6. Complement activation contributes to the anti-methicillin-resistant Staphylococcus aureus effect of natural anti-keratin antibody

Author:
Jingang An; Zhengxiao Li; Yingying Dong; Jiawen Wu; Jianwen Ren
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 142-147
ISSN:
0006-291X
Subject:
antibiotic resistance; antibodies; blood serum; complement; enzyme-linked immunosorbent assay; flow cytometry; fluorescent antibody technique; genes; hemolysis; immune response; immunoglobulin M; in vivo studies; interferon-gamma; interleukin-6; kidneys; lectins; methicillin; methicillin-resistant Staphylococcus aureus; mice; microbial load; neutrophils; public health; spleen; transgenic animals; tumor necrosis factor-alpha
Abstract:
... Methicillin-resistant Staphylococcus aureus (MRSA) remains a major public health problem worldwide because of its strong resistance to a variety of antibiotics. Natural immunoglobulin (Ig) M antibodies have been reported to protect against microbial infections. In the present study, the function of a monoclonal natural anti-keratin antibody IgM (named 3B4) in MRSA infection was evaluated. The bind ...
DOI:
10.1016/j.bbrc.2015.03.182

7. Covalent structure of single-stranded fibrin oligomers cross-linked by FXIIIa

Author:
Mark A. Rosenfeld; Vera B. Leonova; Alexander N. Shchegolikhin; Anna V. Bychkova; Elizaveta A. Kostanova; Marina I. Biryukova
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 408-412
ISSN:
0006-291X
Subject:
crosslinking; dissociation; fibrin; fibrinogen; light scattering; models; polymers; polypeptides; ultracentrifugation; urea
Abstract:
... FXIIIa-mediated isopeptide γ–γ bonds are produced between γ polypeptide chains of adjacent monomeric fibrin. Despite the use of the different methodological approaches there are apparently conflicting ideas regarding the orientation of γ–γ bonds. To identify the orientation of these bonds a novel approach has been applied. It was based on self-assembly of soluble cross-linked fibrin protofibrils o ...
DOI:
10.1016/j.bbrc.2015.04.052

8. Crystal structures of YfiR from Pseudomonas aeruginosa in two redox states

Author:
Xuan Yang; Xiu-an Yang; Min Xu; Lei Zhou; Zusen Fan; Tao Jiang
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 14-20
ISSN:
0006-291X
Subject:
Pseudomonas aeruginosa; anaerobic conditions; biofilm; crystal structure; disulfide bonds; glutathione; membrane proteins; mutagenesis
Abstract:
... YfiBNR is a recently identified c-di-GMP regulatory system involved in bacterial biofilm formation. The periplasmic protein YfiR inhibits the diguanylate cyclase activity of the inner membrane protein YfiN, whereas YfiB in the outer membrane can release this inhibition by sequestration of YfiR. In addition, this system may respond to anoxic conditions via YfiR, although the detailed mechanism is s ...
DOI:
10.1016/j.bbrc.2015.03.160

9. Dysfunction of lipid metabolism in lipodystrophic Seipin-deficient mice

Author:
Mengyu Wang; Mingming Gao; Jiawei Liao; Yingchun Han; Yuhui Wang; George Liu
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 206-210
ISSN:
0006-291X
Subject:
adipose tissue; energy; fasting; fatty liver; homeostasis; humans; hypertriglyceridemia; insulin resistance; lipid metabolism; low density lipoprotein; mice; models; patients; secretion; very low density lipoprotein
Abstract:
... Congenital generalized lipodystrophy (CGL) is characterized by a complete loss of body adipose tissue accompanying dyslipidemia, severe hepatic steatosis and insulin resistance. However, the mechanisms of dyslipidemia and hepatic steatosis are unclear. Here using the lipodystrophic Seipin-deficient mouse (Seipin−/−) model, we found Seipin−/− mice were unable to respond appropriately to a long time ...
DOI:
10.1016/j.bbrc.2015.03.117

10. Effective delivery of recombinant proteins to rod photoreceptors via lipid nanovesicles

Author:
Sabrina Asteriti; Giuditta Dal Cortivo; Valeria Pontelli; Lorenzo Cangiano; Mario Buffelli; Daniele Dell’Orco
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 665-670
ISSN:
0006-291X
Subject:
antibodies; confocal microscopy; encapsulation; lipids; mice; models; phototransduction; physiological response; recombinant proteins; retinal diseases; rods (retina); vision
Abstract:
... The potential of liposomes to deliver functional proteins in retinal photoreceptors and modulate their physiological response was investigated by two experimental approaches. First, we treated isolated mouse retinas with liposomes encapsulating either recoverin, an important endogenous protein operating in visual phototransduction, or antibodies against recoverin. We then intravitrally injected in ...
DOI:
10.1016/j.bbrc.2015.04.088

11. Effects of KRC-108 on the Aurora A activity and growth of colorectal cancer cells

Author:
Hye Jin Chung; Kyeong Ryang Park; Hyo Jeong Lee; Jongkook Lee; Jeong-Hyun Kim; Yong-Chul Kim; Sun-Young Han
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 605-611
ISSN:
0006-291X
Subject:
antineoplastic activity; antineoplastic agents; apoptosis; carcinogenesis; cell cycle checkpoints; cell growth; colorectal neoplasms; cyclins; cytotoxicity; flow cytometry; fluorescent antibody technique; humans; mitosis; neoplasm cells; non-specific serine/threonine protein kinase; prognosis
Abstract:
... Aurora A is involved in regulating multiple steps of mitosis. Over-expression of Aurora A is related to tumorigenesis and poor prognosis. KRC-108 is a novel multi-kinase inhibitor which has anti-tumor activity in vivo. In this study, we identified the inhibitory effects of KRC-108 on Aurora A kinase and growth-inhibitory characteristics of KRC-108. The in vitro kinase activity assay, immunoblot, a ...
DOI:
10.1016/j.bbrc.2015.04.073

12. Epidermis–dermis junction as a novel location for bone marrow-derived cells to reside in response to ionizing radiation

Author:
Junko Okano; Hideto Kojima; Miwako Katagi; Yuki Nakae; Tomoya Terashima; Takahiko Nakagawa; Takeshi Kurakane; Naoki Okamoto; Keita Morohashi; Hiroshi Maegawa; Jun Udagawa
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 695-701
ISSN:
0006-291X
Subject:
bone marrow; green fluorescent protein; inflammation; ionizing radiation; mice; transgenic animals
Abstract:
... Bone marrow-derived cells (BMDCs) can migrate into the various organs in the mice irradiated by ionizing radiation (IR). However, it may not be the case in the skin. While IR is used for bone marrow (BM) transplantation, studying with the epidermal sheets demonstrated that the BMDC recruitment is extraordinarily rare in epidermis in the mouse. Herein, using the chimera mice with BM from green fluo ...
DOI:
10.1016/j.bbrc.2015.04.094

13. Esculetin attenuates alterations in Ang II and acetylcholine mediated vascular reactivity associated with hyperinsulinemia and hyperglycemia

Author:
Almesh Kadakol; Vajir Malek; Santosh Kumar Goru; Anuradha Pandey; Shreyas Bagal; Anil Bhanudas Gaikwad
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 342-347
ISSN:
0006-291X
Subject:
acetylcholine; aorta; high fat diet; hyperglycemia; hyperinsulinemia; immunohistochemistry; insulin; insulin-dependent diabetes mellitus; models; rats; streptozotocin; transforming growth factor beta
Abstract:
... Esculetin (6, 7- dihydroxycoumarin) was found to be protective against hepatic and renal damage associated with Streptozotocin (STZ) induced type 1 diabetes, because of its radical scavenging property. However, there are no reports regarding its effect on vascular dysfunction under hyperinsulinemic and hyperglycemic conditions. Hence, the present study aimed to investigate the effect of esculetin ...
DOI:
10.1016/j.bbrc.2015.04.036

14. Esculetin attenuates receptor activator of nuclear factor kappa-B ligand-mediated osteoclast differentiation through c-Fos/nuclear factor of activated T-cells c1 signaling pathway

Author:
Jong Min Baek; Sun-Hyang Park; Yoon-Hee Cheon; Sung-Jun Ahn; Myeung Su Lee; Jaemin Oh; Ju-Young Kim
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 334-341
ISSN:
0006-291X
Subject:
T-lymphocytes; acid phosphatase; actin; anti-inflammatory activity; bone metabolism; calcitonin receptors; cathepsin K; coculture; integrins; messenger RNA; osteoblasts; osteoclasts; signal transduction; transcription factor NF-kappa B; transmembrane proteins
Abstract:
... Esculetin exerts various biological effects on anti-oxidation, anti-tumors, and anti-inflammation. However, the involvement of esculetin in the bone metabolism process, particularly osteoclast differentiation has not yet been investigated. In the present study, we first confirmed the inhibitory effect of esculetin on receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formati ...
DOI:
10.1016/j.bbrc.2015.04.034

15. Hypoxia potentiates tumor necrosis factor-α induced expression of inducible nitric oxide synthase and cyclooxygenase-2 in white and brown adipocytes

Author:
Indranil Bhattacharya; Ana Pérez Domínguez; Katja Drägert; Rok Humar; Elvira Haas; Edouard J. Battegay
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 287-292
ISSN:
0006-291X
Subject:
adipocytes; adipose tissue; biogenesis; gene expression regulation; homeostasis; hypoxia; inducible nitric oxide synthase; inflammation; mitochondria; necrosis; nitrites; normoxia; obesity; prostaglandin synthase; transcription (genetics); transcription factors; tumor necrosis factor-alpha
Abstract:
... Obesity involves hypoxic adipose tissue and low-grade chronic inflammation. We investigated the impact of hypoxia on inflammatory response to TNF-α in white and brown adipocytes. In response to TNF-α, the expression of the inducible enzymes iNOS and COX-2 was prominently and selectively potentiated during hypoxia while only moderately under normoxia. Levels of their products, nitrite and prostagla ...
DOI:
10.1016/j.bbrc.2015.04.020

16. Identification and characterization of three novel hemocyte-specific promoters in silkworm Bombyx mori

Author:
Kui Zhang; Shuang Yu; Jingjing Su; Man Xu; Peng Tan; Yajun Zhang; Zhonghuai Xiang; Hongjuan Cui
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 102-108
ISSN:
0006-291X
Subject:
metabolism; hematopoiesis; growth and development; innate immunity; metamorphosis; silkworms; fat body; silk glands; Bombyx mori; introns; promoter regions; hemocytes
Abstract:
... Insect hemocytes play essential roles in the metabolism, metamorphosis and immunity, which are closely related events of growth and development. Here, four novel hemocyte-specific genes were obtained and conformed in our study, namely, Bmintβ2, Bmintβ3, BmCatO, and BmSw04862, respectively. Subsequently, their promoter sequences were cloned, and their activity in hemocytes, fat body, and silk gland ...
DOI:
10.1016/j.bbrc.2015.03.176

17. Intensified autophagy compromises the efficacy of radiotherapy against prostate cancer

Author:
Michael I. Koukourakis; Dimitra Kalamida; Achilleas Mitrakas; Stamatia Pouliliou; Sofia Kalamida; Efthimios Sivridis; Alexandra Giatromanolaki
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 268-274
ISSN:
0006-291X
Subject:
autophagy; biopsy; complement; confocal microscopy; fluorescent antibody technique; lysosomes; neoplasm cells; patients; phenotype; prostate gland; prostatic neoplasms; proteins; radiation resistance; radiotherapy; relapse; risk
Abstract:
... Radiotherapy is an equivalent alternative or complement to radical prostatectomy, with high therapeutic efficacy. High risk patients, however, experience high relapse rates, so that research on radio-sensitization is the most evident route to improve curability of this common disease.In the current study we investigated the autophagic activity in a series of patients with localized prostate tumors ...
DOI:
10.1016/j.bbrc.2015.04.014

18. In vivo characterization of human myofibrillar myopathy genes in zebrafish

Author:
John B. Bührdel; Sofia Hirth; Mirjam Keßler; Sören Westphal; Monika Forster; Linda Manta; Gerhard Wiche; Benedikt Schoser; Joachim Schessl; Rolf Schröder; Christoph S. Clemen; Ludwig Eichinger; Dieter O. Fürst; Peter F.M. van der Ven; Wolfgang Rottbauer; Steffen Just
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 217-223
ISSN:
0006-291X
Subject:
Danio rerio; genes; heart; heart failure; human diseases; humans; models; muscular diseases; mutation; patients; phenotype; quality of life; skeletal muscle
Abstract:
... Myofibrillar myopathies (MFM) are progressive diseases of human heart and skeletal muscle with a severe impact on life quality and expectancy of affected patients. Although recently several disease genes for myofibrillar myopathies could be identified, today most genetic causes and particularly the associated mechanisms and signaling events that lead from the mutation to the disease phenotype are ...
DOI:
10.1016/j.bbrc.2015.03.149

19. Localization of the dominant non-enzymatic intermolecular cross-linking sites on fibrous collagen

Author:
Hiroko Chiue; Tsutako Yamazoye; Sueo Matsumura
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 445-449
ISSN:
0006-291X
Subject:
collagen; crosslinking; physicochemical properties; reducing sugars; ribose
Abstract:
... Previous studies have shown that fibrous collagen undergoes intermolecular cross-linking at multiple sites of the elongated triple-helical regions among adjacent juxtaposed collagen molecules on incubation with a very high concentration of reducing sugar such as 200 mM ribose, and the similarity of the changes in its physicochemical properties to that of senescent collagen aged in vivo has been em ...
DOI:
10.1016/j.bbrc.2015.04.011

20. Locomotion of bacteria in liquid flow and the boundary layer effect on bacterial attachment

Author:
Chao Zhang; Qiang Liao; Rong Chen; Xun Zhu
Source:
Biochemical and biophysical research communications 2015 v.461 pp. 671-676
ISSN:
0006-291X
Subject:
bacteria; bacterial adhesion; bacterial motility; biofilm; locomotion; mathematical models
Abstract:
... The formation of biofilm greatly affects the performance of biological reactors, which highly depends on bacterial swimming and attachment that usually takes place in liquid flow. Therefore, bacterial swimming and attachment on flat and circular surfaces with the consideration of flow was studied experimentally. Besides, a mathematical model comprehensively combining bacterial swimming and motion ...
DOI:
10.1016/j.bbrc.2015.04.089