U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal name Biochemical and biophysical research communications Remove constraint Journal name: Biochemical and biophysical research communications Publication year rev 7986-2014 Remove constraint Publication year rev: 7986-2014 Source 2014 v.448 Remove constraint Source: 2014 v.448
Start Over

Search

80  Search Results

« Previous | 1 - 20 of 80 |
Number of results to display per page

Search Results

1. Analysis of the stem cell characteristics of adult stem cells from Arbas white Cashmere goat

Author:
Yu Ren; Haiqing Wu; Xiao Wang; Na Xue; Hao Liang; Dongjun Liu
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 121-128
ISSN:
0006-291X
Subject:
GATA transcription factors; Western blotting; adult stem cells; adults; appendages; biomarkers; bone marrow; embryonic stem cells; gene expression; goats; immunocytochemistry; in vitro culture; interleukin-6; messenger RNA; mice; neoplasms; reverse transcriptase polymerase chain reaction
Abstract:
... Studies have shown that multipotent adult stem cells possess differentiation characteristics similar to embryonic stem cells and pluripotent stem cells. We aimed to explore these similarities further by examining the expression of the pluripotency and stemness biomarkers, AKP, IL-6, Nanog, Oct-4, Rex-1, Sox-2 and TERT, as well as the triploblastic biomarkers, Sox-1, Myod1 and Gata-6 in adipose-der ...
DOI:
10.1016/j.bbrc.2013.12.044

2. Aquaporin 5 increases keratinocyte-derived chemokine expression and NF-κB activity through ERK activation

Author:
Yuima Sakamoto; Akinori Hisatsune; Hiroshi Katsuki; Ichiro Horie; Yoichiro Isohama
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 355-360
ISSN:
0006-291X
Subject:
aquaporins; cell growth; chemoattractants; chemokines; epithelial cells; inflammation; keratinocytes; lungs; messenger RNA; mitogen-activated protein kinase; phosphorylation; small interfering RNA; transcription factor NF-kappa B; water metabolism
Abstract:
... Aquaporin-5 (AQP5) is a water-selective channel protein that is expressed in submucosal glands and alveolar epithelial cells in the lungs. Recent studies have revealed that AQPs regulate not only water metabolism, but also some cellular functions such as cell growth and migration. Here, we report the role of AQP5 in inflammatory responses. In MLE-12 cells, knockdown of AQP5 using siRNA (10–50nM) a ...
DOI:
10.1016/j.bbrc.2014.04.047

3. Automated quantification of FISH signals in urinary cells enables the assessment of chromosomal aberration patterns characteristic for bladder cancer

Author:
Christina U. Köhler; Laura Martin; Nadine Bonberg; Thomas Behrens; Thomas Deix; Katharina Braun; Joachim Noldus; Karl-Heinz Jöckel; Raimund Erbel; Florian Sommerer; Andrea Tannapfel; Volker Harth; Heiko U. Käfferlein; Thomas Brüning
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 467-472
ISSN:
0006-291X
Subject:
centromeres; chromosome aberrations; computer software; cost effectiveness; diagnostic techniques; epidemiological studies; fluorescence in situ hybridization; patients; sediments; urinary bladder neoplasms; urine
Abstract:
... Targeting the centromeres of chromosomes 3, 7, 17 (CEP3, 7, 17) and the 9p21-locus (LSI9p21) for diagnosing bladder cancer (BC) is time- and cost-intensive and requires a manual investigation of the sample by a well-trained investigator thus overall limiting its use in clinical diagnostics and large-scaled epidemiological studies. Here we introduce a new computer-assisted FISH spot analysis tool e ...
DOI:
10.1016/j.bbrc.2014.04.137

4. Bile acids override steatosis in farnesoid X receptor deficient mice in a model of non-alcoholic steatohepatitis

Author:
Weibin Wu; Xijun Liu; Xiaomin Peng; Ruyi Xue; Lingling Ji; Xizhong Shen; She Chen; Jianxin Gu; Si Zhang
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 50-55
ISSN:
0006-291X
Subject:
alanine transaminase; aspartate transaminase; bile acids; blood serum; diet; fatty acids; fatty liver; fibrosis; gene expression; histology; homeostasis; hormone receptors; inflammation; liver; mice; models; pathogenesis; triacylglycerols
Abstract:
... Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases, and the pathogenesis is still not well known. The farnesoid X receptor (FXR) is a member of the nuclear hormone receptor superfamily and plays an essential role in maintaining bile acid and lipid homeostasis. In this study, we study the role of FXR in the pathogenesis of NFALD. We found that FXR deficient (FXR−/−) ...
DOI:
10.1016/j.bbrc.2014.04.048

5. Cancer cells recovering from damage exhibit mitochondrial restructuring and increased aerobic glycolysis

Author:
Shin Akakura; Elena Ostrakhovitch; Reiko Sanokawa-Akakura; Siamak Tabibzadeh
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 461-466
ISSN:
0006-291X
Subject:
cell proliferation; glycolysis; mitochondria; neoplasm cells; neoplasms; oxidative phosphorylation
Abstract:
... Instead of relying on mitochondrial oxidative phosphorylation, most cancer cells rely heavily on aerobic glycolysis, a phenomenon termed as “the Warburg effect”. We considered that this effect is a direct consequence of damage which persists in cancer cells that recover from damage. To this end, we studied glycolysis and rate of cell proliferation in cancer cells that recovered from severe damage. ...
DOI:
10.1016/j.bbrc.2014.04.138

6. Characterization of essential genes by topological properties in the perturbation sensitivity network

Author:
Lei Yang; Jizhe Wang; Huiping Wang; Yingli Lv; Yongchun Zuo; Wei Jiang
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 473-479
ISSN:
0006-291X
Subject:
Saccharomyces cerevisiae; essential genes; gene expression regulation; topology; transcription (genetics)
Abstract:
... Genes that are indispensable for survival are called essential genes. In recent years, the analysis of essential genes has become extremely important for understanding the way a cell functions. With the advent of large-scale gene expression profiling technologies, it is now possible to profile transcriptional changes in the entire genome of Saccharomyces cerevisiae. Notwithstanding the accumulatio ...
DOI:
10.1016/j.bbrc.2014.04.136

7. The Cj0588 protein is a Campylobacter jejuni RNA methyltransferase

Author:
Agnieszka Sałamaszyńska-Guz; Bartłomiej Taciak; Agnieszka Kwiatek; Danuta Klimuszko
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 298-302
ISSN:
0006-291X
Subject:
Campylobacter jejuni; bacteria; capreomycin; genes; methylation; methyltransferases; protein subunits; ribosomal RNA; ribosomal proteins; ribosomes; virulence
Abstract:
... TlyA proteins belong to 2′-O-methyltransferases. Methylation is a common posttranscriptional RNA modification. The Campylobacter jejuni Cj0588 protein belongs to the TlyAI protein family and is a rRNA methyltransferase. Methylation of ribosomal RNA catalyzed by Cj0588 appears to have an impact on the biology of the cell. Presence of the cj0588 gene in bacteria appears to be important for ribosome ...
DOI:
10.1016/j.bbrc.2014.04.104

8. Crystal structure and biochemical properties of the (S)-3-hydroxybutyryl-CoA dehydrogenase PaaH1 from Ralstonia eutropha

Author:
Jieun Kim; Jeong Ho Chang; Kyung-Jin Kim
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 163-168
ISSN:
0006-291X
Subject:
Cupriavidus necator; adenosine diphosphate; biofuels; biosynthesis; butanol; catalytic activity; crystal structure; dimerization; site-directed mutagenesis
Abstract:
... 3-Hydroxybutyryl-CoA dehydrogenase is an enzyme involved in the synthesis of the biofuel n-butanol by converting acetoacetyl-CoA to 3-hydroxybutyryl-CoA. To investigate the molecular mechanism of n-butanol biosynthesis, we determined crystal structures of the Ralstonia eutropha-derived 3-hydroxybutyryl-CoA dehydrogenase (RePaaH1) in complex with either its cofactor NAD+ or its substrate acetoacety ...
DOI:
10.1016/j.bbrc.2014.04.101

9. Crystal structure of the NHERF1 PDZ2 domain in complex with the chemokine receptor CXCR2 reveals probable modes of PDZ2 dimerization

Author:
Joshua Holcomb; Yuanyuan Jiang; Xiaoqing Guan; Laura Trescott; Guorong Lu; Yuning Hou; Shuo Wang; Joseph Brunzelle; Nualpun Sirinupong; Chunying Li; Zhe Yang
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 169-174
ISSN:
0006-291X
Subject:
CXCR2 receptor; chemotaxis; crystal structure; dimerization; hydrogen bonding; hydrophobicity; neutrophils
Abstract:
... The formation of CXCR2–NHERF1–PLCβ2 macromolecular complex in neutrophils regulates CXCR2 signaling and plays a key role in neutrophil chemotaxis and transepithelial neutrophilic migration. However, NHERF1 by itself, with only two PDZ domains, has a limited capacity in scaffolding the multiprotein-complex formation. Here we report the crystal structure of the NHERF1 PDZ2 domain in complex with the ...
DOI:
10.1016/j.bbrc.2014.04.085

10. Curcumin-induced melanoma cell death is associated with mitochondrial permeability transition pore (mPTP) opening

Author:
Ying Qiu; Teng Yu; Wei Wang; Kun Pan; Dongmei Shi; Hui Sun
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 15-21
ISSN:
0006-291X
Subject:
apoptosis; ceramides; curcumin; cyclosporine; cytochrome c; cytotoxicity; melanoma; membrane potential; mitochondria; mitochondrial membrane; permeability; small interfering RNA
Abstract:
... Here we studied the role of mitochondrial permeability transition pore (mPTP) opening in curcumin’s cytotoxicity in melanoma cells. In cultured WM-115 melanoma cells, curcumin induced mitochondrial membrane potential (MPP) decrease, cyclophilin-D (CyPD)-adenine nucleotide translocator 1 (ANT-1) (two mPTP components) mitochondrial association and cytochrome C release, indicating mPTP opening. The m ...
DOI:
10.1016/j.bbrc.2014.04.024

11. Deafness induced by Connexin 26 (GJB2) deficiency is not determined by endocochlear potential (EP) reduction but is associated with cochlear developmental disorders

Author:
Jin Chen; Jing Chen; Yan Zhu; Chun Liang; Hong-Bo Zhao
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 28-32
ISSN:
0006-291X
Subject:
animal models; brain stem; deafness; mice; mutation
Abstract:
... Connexin 26 (Cx26, GJB2) mutations are the major cause of hereditary deafness and are responsible for >50% of nonsyndromic hearing loss. Mouse models show that Cx26 deficiency can cause congenital deafness with cochlear developmental disorders, hair cell degeneration, and the reduction of endocochlear potential (EP) and active cochlear amplification. However, the underlying deafness mechanism stil ...
DOI:
10.1016/j.bbrc.2014.04.016
PubMed:
24732355
PubMed Central:
PMC4105360

12. Dephosphorylation of Orc2 by protein phosphatase 1 promotes the binding of the origin recognition complex to chromatin

Author:
Kyung Yong Lee; June Sung Bae; Sangwook Yoon; Deog Su Hwang
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 385-389
ISSN:
0006-291X
Subject:
chromatin; consensus sequence; cyclins; dephosphorylation; dissociation; humans; interphase; phosphorylation; replication origin
Abstract:
... Phosphorylation of Orc2, one of the six subunits of the origin recognition complex (ORC), by cyclin A/CDK2 during S phase leads to the dissociation of Orc2, Orc3, Orc4, and Orc5 subunits (Orc2–5) from human chromatin and replication origins. Dephosphorylation of the phosphorylated Orc2 by protein phosphatase 1 (PP1) is accompanied by the binding of the dissociated subunits to chromatin. Here we sh ...
DOI:
10.1016/j.bbrc.2014.04.109

13. Dimethyl fumarate inhibits the expression and function of hypoxia-inducible factor-1α (HIF-1α)

Author:
Guangzong Zhao; Yao Liu; Jun Fang; Yunzhen Chen; Huazhuang Li; Kehai Gao
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 303-307
ISSN:
0006-291X
Subject:
dimethyl fumarate; gene expression regulation; genes; heat shock proteins; hypoxia; hypoxia-inducible factor 1; interleukin-8; protein kinase C; sclerosis; therapeutics; vascular endothelial growth factors
Abstract:
... Osteocyte hypoxia has been induced by skeletal unloading and fracture. Hypoxia-dependent regulation of gene expression is mediated by hypoxia-sensitive transcription factors such as hypoxia-inducible factor-1α (HIF-1α). Dimethyl fumarate (DMF) is a recently approved first-line therapy for multiple sclerosis. However, the role of DMF in regulating HIF-1α expression and function has not been evaluat ...
DOI:
10.1016/j.bbrc.2014.02.062

14. Down regulation of brain cellular prion protein in an animal model of insulin resistance: Possible implication in increased prevalence of stroke in pre-diabetics/diabetics

Author:
Nam Pham; Arti Dhar; Siavash Khalaj; Kash Desai; Changiz Taghibiglou
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 151-156
ISSN:
0006-291X
Subject:
animal models; apoptosis; brain; diabetes; diet; glutamate receptors; insulin receptors; insulin resistance; messenger RNA; neurons; neuroprotective effect; obesity; patients; phosphatidylinositol 3-kinase; phosphorylation; prions; prognosis; protein synthesis; rats; risk; serine; stroke; tyrosine
Abstract:
... The risk of stroke is drastically increased in diabetic and pre-diabetic patients. The worldwide spread of obesity and insulin resistance increases the incidence of stroke. The direct effect of insulin resistance, as it pertains to stroke, on the central nervous system is not well understood. Since one of the physiological functions of the cellular prion protein (PrPC) is neuroprotection, we studi ...
DOI:
10.1016/j.bbrc.2014.04.071

15. Downregulation of SCAI enhances glioma cell invasion and stem cell like phenotype by activating Wnt/β-catenin signaling

Author:
Xiangrong Chen; Weipeng Hu; Baoyuan Xie; Hongzhi Gao; Chaoyang Xu; Junyan Chen
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 206-211
ISSN:
0006-291X
Subject:
messenger RNA; phenotype; humans; gene overexpression; neoplasms; stem cells; neoplasm cells; gene expression regulation; tissues
Abstract:
... SCAI (suppressor of cancer cell invasion), has been recently characterized as a novel tumor suppressor that inhibits the invasive migration of several human tumor cells. However, the expression pattern, biological role and molecular mechanism of SCAI in human glioma remain unknown. In this study, we found that levels of SCAI protein and mRNA expression were significantly downregulated in glioma ti ...
DOI:
10.1016/j.bbrc.2014.04.098

16. Dysfunction of mitochondrial dynamics in the brains of scrapie-infected mice

Author:
Hong-Seok Choi; Yeong-Gon Choi; Hae-Young Shin; Jae-Min Oh; Jeong-Ho Park; Jae-Il Kim; Richard I. Carp; Eun-Kyoung Choi; Yong-Sun Kim
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 157-162
ISSN:
0006-291X
Subject:
animal models; cerebral cortex; hippocampus; histopathology; mice; mitochondria; neurodegenerative diseases; oxidative stress; prion diseases; proteins
Abstract:
... Mitochondrial dysfunction is a common and prominent feature of many neurodegenerative diseases, including prion diseases; it is induced by oxidative stress in scrapie-infected animal models. In previous studies, we found swelling and dysfunction of mitochondria in the brains of scrapie-infected mice compared to brains of controls, but the mechanisms underlying mitochondrial dysfunction remain uncl ...
DOI:
10.1016/j.bbrc.2014.04.069

17. Effect of thymic stromal lymphopoietin on MUC5B expression in human airway epithelial cells

Author:
Chang Hoon Bae; Yoon Seok Choi; Si-Youn Song; Yong-Dae Kim
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 231-235
ISSN:
0006-291X
Subject:
T-lymphocytes; enzyme immunoassays; epithelial cells; gene expression; genes; humans; interleukins; messenger RNA; mitogen-activated protein kinase; mucins; nose; phosphorylation; quantitative polymerase chain reaction; respiratory tract diseases; reverse transcriptase polymerase chain reaction; signal transduction; small interfering RNA
Abstract:
... Thymic stromal lymphopoietin (TSLP) is an interleukin 7-like cytokine and a potent factor for B- and T-cell growth and differentiation. Recent studies have demonstrated an association of TSLP with allergic and inflammatory airway diseases. However, no study on the effect of TSLP on expression of mucin genes in airway epithelial cells has been reported. Therefore, the effects and brief signaling pa ...
DOI:
10.1016/j.bbrc.2014.04.100

18. Efficient isolation and elution of cellular proteins using aptamer-mediated protein precipitation assay

Author:
Kiseok Kim; SeungJin Lee; Sungho Ryu; Dongil Han
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 114-119
ISSN:
0006-291X
Subject:
DNA; antigen detection; binding proteins; cost effectiveness; epidermal growth factor receptors; humans; insulin receptors; oligonucleotides; protein-protein interactions
Abstract:
... Protein precipitation is one of the most widely used methods for antigen detection and purification in biological research. We developed a reproducible aptamer-mediated magnetic protein precipitation method that is able to efficiently capture, purify and isolate the target proteins. We discovered DNA aptamers having individually high affinity and specificity against human epidermal growth factor r ...
DOI:
10.1016/j.bbrc.2014.04.086

19. Enhanced binding of calmodulin to RyR2 corrects arrhythmogenic channel disorder in CPVT-associated myocytes

Author:
Masakazu Fukuda; Takeshi Yamamoto; Shigehiko Nishimura; Takayoshi Kato; Wakako Murakami; Akihiro Hino; Makoto Ono; Hiroki Tateishi; Testuro Oda; Shinichi Okuda; Shigeki Kobayashi; Noritaka Koseki; Hiroyuki Kyushiki; Masafumi Yano
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 1-7
ISSN:
0006-291X
Subject:
animal models; calcium; calmodulin; cyclic AMP; mutation; myocytes
Abstract:
... Calmodulin (CaM) plays a key role in modulating channel gating in ryanodine receptor (RyR2). Here, we investigated (a) the pathogenic role of CaM in the channel disorder in CPVT and (b) the possibility of correcting the CPVT-linked channel disorder, using knock-in (KI) mouse model with CPVT-associated RyR2 mutation (R2474S).Transmembrane potentials were recorded in whole cell current mode before a ...
DOI:
10.1016/j.bbrc.2014.03.152

20. Functional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency

Author:
Tetsuhiko Yasuno; Kenji Osafune; Hidetoshi Sakurai; Isao Asaka; Akihito Tanaka; Seiji Yamaguchi; Kenji Yamada; Hirofumi Hitomi; Sayaka Arai; Yuko Kurose; Yasuki Higaki; Mizuki Sudo; Soichi Ando; Hitoshi Nakashima; Takao Saito; Hidetoshi Kaneoka
Source:
Biochemical and biophysical research communications 2014 v.448 pp. 175-181
ISSN:
0006-291X
Subject:
Food and Agriculture Organization; beta oxidation; carnitine palmitoyltransferase; disease models; drugs; fibroblasts; genetic disorders; heat stress; humans; induced pluripotent stem cells; lipemic effect; long chain fatty acids; mitochondria; muscle development; myocytes; pathogenesis; patients; phenotype; renal failure; rhabdomyolysis; skeletal muscle; transcription factors
Abstract:
... Carnitine palmitoyltransferase II (CPT II) deficiency is an inherited disorder involving β-oxidation of long-chain fatty acids (FAO), which leads to rhabdomyolysis and subsequent acute renal failure. The detailed mechanisms of disease pathogenesis remain unknown; however, the availability of relevant human cell types for investigation, such as skeletal muscle cells, is limited, and the development ...
DOI:
10.1016/j.bbrc.2014.04.084