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You searched for: Journal Biochemical and biophysical research communications Remove constraint Journal: Biochemical and biophysical research communications Publication Year 2014 Remove constraint Publication Year: 2014 Source 2014 v.449 Remove constraint Source: 2014 v.449
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1. Antibody-modified lipid nanoparticles for selective delivery of siRNA to tumors expressing membrane-anchored form of HB-EGF

Author:
Okamoto, Ayaka; Asai, Tomohiro; Kato, Hiroki; Ando, Hidenori; Minamino, Tetsuo; Mekada, Eisuke; Oku, Naoto
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 460-465
ISSN:
0006-291X
Subject:
antibodies; breast neoplasms; encapsulation; gene silencing; humans; lipids; messenger RNA; nanoparticles; neoplasm cells; small interfering RNA
Abstract:
... An Fab’ antibody against heparin-binding epidermal growth factor-like growth factor (HB-EGF) was applied to achieve advanced tumor-targeted delivery of siRNA. Lipid nanoparticles (LNP) encapsulating siRNA (LNP-siRNA) were prepared, pegylated, and surface modified with Fab’ fragments of anti-HB-EGF antibody (αHB-EGF LNP-siRNA). αHB-EGF LNP-siRNA showed high-binding affinity to recombinant human HB- ...
DOI:
10.1016/j.bbrc.2014.05.043

2. A sensitive assay for the biosynthesis and secretion of MANF using NanoLuc activity

Author:
Norisada, Junpei; Hirata, Yoko; Amaya, Fumimasa; Kiuchi, Kazutoshi; Oh-hashi, Kentaro
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 483-489
ISSN:
0006-291X
Subject:
biosynthesis; blood serum; cell death; culture media; diabetes; gene overexpression; genes; ischemia; luciferase; mutants; neurodegenerative diseases; neurons; potassium; screening; secretion; signal peptide; transcription (genetics)
Abstract:
... Mesencephalic astrocyte-derived neurotrophic factor (MANF) has been reported to prevent neuronal cell death caused by certain stimuli. Accordingly, the molecular features of MANF have been intensively investigated since the reporting of its cytoprotective actions. In addition to the characterization of the transcriptional regulation of MANF under pathophysiological conditions, it is important to u ...
DOI:
10.1016/j.bbrc.2014.05.031

3. Cancer–testis antigen HCA587/MAGE-C2 interacts with BS69 and promotes its degradation in the ubiquitin–proteasome pathway

Author:
Hao, Jiaqing; Shen, Rui; Li, Yan; Zhang, Yu; Yin, Yanhui
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 386-391
ISSN:
0006-291X
Subject:
antigens; genes; interleukin-6; males; mass spectrometry; neoplasms; precipitin tests; sequence homology; tissues; transcription factor NF-kappa B; ubiquitination
Abstract:
... HCA587, also known as MAGE-C2, belonging to the MAGE gene family which is characterized by a conserved MAGE Homology Domain, is active in various types of tumors and silent in normal tissues except in male germ-line cells. The biological function of HCA587 is largely unknown. To analyze it, we attempted to identify protein partners of HCA587. We immunopurified HCA587-containing complex from HEK293 ...
DOI:
10.1016/j.bbrc.2014.05.078

4. Characterization of the human oncogene SCL/TAL1 interrupting locus (Stil) mediated Sonic hedgehog (Shh) signaling transduction in proliferating mammalian dopaminergic neurons

Author:
Sun, Lei; Carr, Aprell L.; Li, Ping; Lee, Jessica; McGregor, Mary; Li, Lei
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 444-448
ISSN:
0006-291X
Subject:
Parkinson disease; apoptosis; cell cycle; cell proliferation; cell viability; gene expression regulation; gene overexpression; humans; loci; neoplasms; neurons; oncogenes; plasmids; precipitin tests; proliferating cell nuclear antigen; signal transduction; transfection
Abstract:
... The human oncogene SCL/TAL1 interrupting locus (Stil) is highly conserved in all vertebrate species. In humans, the expression of Stil is involved in cancer cell survival, apoptosis and proliferation. In this research, we investigated the roles of Stil expression in cell proliferation of mammalian dopaminergic (DA) PC12 cells. Stil functions through the Sonic hedgehog (Shh) signal transduction pat ...
DOI:
10.1016/j.bbrc.2014.05.048

5. Differential expression of extracellular-signal-regulated kinase 5 (ERK5) in normal and degenerated human nucleus pulposus tissues and cells

Author:
Liang, Weiguo; Fang, Dejian; Ye, Dongping; Zou, Longqiang; Shen, Yan; Dai, Libing; Xu, Jiake
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 466-470
ISSN:
0006-291X
Subject:
Western blotting; apoptosis; collagen; gene expression; gene expression regulation; genetic markers; humans; immunohistochemistry; mitogen-activated protein kinase; necrosis; small interfering RNA; staining; tissues; tumor necrosis factor-alpha
Abstract:
... Extracellular-signal-regulated kinase 5 (ERK5) is a member of the mitogen-activated protein kinase (MAPK) family and regulates a wide variety of cellular processes such as proliferation, differentiation, necrosis, apoptosis and degeneration. However, the expression of ERK5 and its role in degenerated human nucleus pulposus (NP) is hitherto unknown. In this study, we observed the differential expre ...
DOI:
10.1016/j.bbrc.2014.05.042

6. Folate in demethylation: The crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate

Author:
Luka, Zigmund; Pakhomova, Svetlana; Loukachevitch, Lioudmila V.; Newcomer, Marcia E.; Wagner, Conrad
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 392-398
ISSN:
0006-291X
Subject:
binding sites; choline; crystal structure; formaldehyde; humans; hydrogen peroxide; mitochondria; mutation; rats; tetrahydrofolic acid
Abstract:
... Dimethylglycine dehydrogenase (DMGDH) is a mammalian mitochondrial enzyme which plays an important role in the utilization of methyl groups derived from choline. DMGDH is a flavin containing enzyme which catalyzes the oxidative demethylation of dimethylglycine in vitro with the formation of sarcosine (N-methylglycine), hydrogen peroxide and formaldehyde. DMGDH binds tetrahydrofolate (THF) in vivo, ...
DOI:
10.1016/j.bbrc.2014.05.064
PubMed:
24858690
PubMed Central:
PMC4113215

7. Fusaric acid modulates Type Three Secretion System of Salmonella enterica serovar Typhimurium

Author:
Li, Jianfang; Sun, Weiyang; Guo, Zhixing; Lu, Chunhua; Shen, Yuemao
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 455-459
ISSN:
0006-291X
Subject:
Salmonella Typhimurium; anti-infective agents; antibiotic resistance; drugs; fusaric acid; gene overexpression; genes; microbial growth; pathogens; secretion; type III secretion system; viability; virulence
Abstract:
... Natural small-molecule products are promising lead compounds for developing a generation of novel antimicrobials agents to meet the challenge of antibiotic-resistant pathogens. To facilitate the search for novel anti-virulence agents, we chose a virulence factor of Type Three Secretion System (T3SS) as a drug target to screen candidates from a small-molecule library in our laboratory. This study d ...
DOI:
10.1016/j.bbrc.2014.05.044

8. Global impact of Salmonella type III secretion effector SteA on host cells

Author:
Cardenal-Muñoz, Elena; Gutiérrez, Gabriel; Ramos-Morales, Francisco
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 419-424
ISSN:
0006-291X
Subject:
Gram-negative bacteria; Salmonella enterica; bacteremia; bioinformatics; cell adhesion; cell proliferation; cytotoxicity; epithelial cells; extracellular matrix; gastroenteritis; genes; humans; pathogenicity islands; pathogens; protein phosphorylation; protein-serine-threonine kinases; serine; signal transduction; threonine; type III secretion system; typhoid fever; vacuoles; virulence
Abstract:
... Salmonella enterica is a Gram-negative bacterium that causes gastroenteritis, bacteremia and typhoid fever in several animal species including humans. Its virulence is greatly dependent on two type III secretion systems, encoded in pathogenicity islands 1 and 2. These systems translocate proteins called effectors into eukaryotic host cell. Effectors interfere with host signal transduction pathways ...
DOI:
10.1016/j.bbrc.2014.05.056

9. IL- 6, A1 and A2aR: A crosstalk that modulates BDNF and induces neuroprotection

Author:
Perígolo-Vicente, Rafael; Ritt, Karen; Gonçalves-de-Albuquerque, Cassiano Felippe; Castro-Faria-Neto, Hugo Caire; Paes-de-Carvalho, Roberto; Giestal-de-Araujo, Elizabeth
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 477-482
ISSN:
0006-291X
Subject:
adenosine; cell death; diabetes; ganglia; glaucoma; interleukin-6; mixed culture; neuroprotective effect; purinergic receptors; retinal diseases
Abstract:
... Several diseases are related to retinal ganglion cell death, such as glaucoma, diabetes and other retinopathies. Many studies have attempted to identify factors that could increase neuroprotection after axotomy of these cells. Interleukin-6 has been shown to be able to increase the survival and regeneration of retinal ganglion cells (RGC) in mixed culture as well as in vivo. In this work we show t ...
DOI:
10.1016/j.bbrc.2014.05.036

10. Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-κB activation

Author:
Yun, Hong Shik; Baek, Jeong-Hwa; Yim, Ji-Hye; Lee, Su-Jae; Lee, Chang-Woo; Song, Jie-Young; Um, Hong-Duck; Park, Jong Kuk; Park, In-Chul; Hwang, Sang-Gu
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 471-476
ISSN:
0006-291X
Subject:
transcription factor NF-kappa B; phenotype; lung neoplasms; biomarkers; radiation resistance; reactive oxygen species; new drugs; neoplasm cells; apoptosis
Abstract:
... We previously identified hepatoma-derived growth factor-related protein-3 (HRP-3) as a radioresistant biomarker in p53 wild-type A549 cells and found that p53-dependent induction of the PUMA pathway was a critical event in regulating the radioresistant phenotype. Here, we found that HRP-3 knockdown regulates the radioresistance of p53-null H1299 cells through a distinctly different molecular mecha ...
DOI:
10.1016/j.bbrc.2014.05.039

11. Knockdown of TWIST1 enhances arsenic trioxide- and ionizing radiation-induced cell death in lung cancer cells by promoting mitochondrial dysfunction

Author:
Seo, Sung-Keum; Kim, Jae-Hee; Choi, Ha-Na; Choe, Tae-Boo; Hong, Seok-Il; Yi, Jae-Youn; Hwang, Sang-Gu; Lee, Hyun-Gyu; Lee, Yun-Han; Park, In-Chul
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 490-495
ISSN:
0006-291X
Subject:
mitochondrial membrane; proteins; cell death; lung neoplasms; ionization; reactive oxygen species; arsenic; ionizing radiation; therapeutics; metastasis; mitochondria; neoplasm cells; small interfering RNA; epithelium; gene expression regulation; drug resistance
Abstract:
... TWIST1 is implicated in the process of epithelial mesenchymal transition, metastasis, stemness, and drug resistance in cancer cells, and therefore is a potential target for cancer therapy. In the present study, we found that knockdown of TWIST1 by small interfering RNA (siRNA) enhanced arsenic trioxide (ATO)- and ionizing radiation (IR)-induced cell death in non-small-cell lung cancer cells. Inter ...
DOI:
10.1016/j.bbrc.2014.05.030

12. MicroRNA-497 inhibition of ovarian cancer cell migration and invasion through targeting of SMAD specific E3 ubiquitin protein ligase 1

Author:
Wang, Wei; Ren, Fang; Wu, Qinghua; Jiang, Dazhi; Li, Hongjun; Peng, Zheng; Wang, Jinglu; Shi, Huirong
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 432-437
ISSN:
0006-291X
Subject:
animal ovaries; cell movement; death; metastasis; neoplasm cells; ovarian neoplasms; patients; therapeutics; tissues; ubiquitin-protein ligase
Abstract:
... Ovarian cancer is the leading cause of death from gynecological malignancies worldwide. Understanding the molecular mechanism underlying ovarian cancer progression facilitates the development of promising strategy for ovarian cancer therapy. Previously, we observed frequent down-regulation of miR-497 expression in ovarian cancer tissues. In this study, we investigated the role of miR-497 in ovaria ...
DOI:
10.1016/j.bbrc.2014.05.053

13. Pharmacological assessment of methamphetamine-induced behavioral hyperactivity mediated by dopaminergic transmission in planarian Dugesia japonica

Author:
Tashiro, Natsuka; Nishimura, Kaneyasu; Daido, Kanako; Oka, Tomoe; Todo, Mio; Toshikawa, Asami; Tsushima, Jun; Takata, Kazuyuki; Ashihara, Eishi; Yoshimoto, Kanji; Agata, Kiyokazu; Kitamura, Yoshihisa
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 412-418
ISSN:
0006-291X
Subject:
Dugesia; RNA interference; antagonists; brain; dopamine; fluorescent antibody technique; freshwater; genes; in situ hybridization; locomotion; messenger RNA; tyrosine 3-monooxygenase
Abstract:
... The freshwater planarian Dugesia japonica has a simple central nervous system (CNS) and can regenerate complete organs, even a functional brain. Recent studies demonstrated that there is a great variety of neuronal-related genes, specifically expressed in several domains of the planarian brain. We identified a planarian dat gene, named it D. japonica dopamine transporter (Djdat), and analyzed its ...
DOI:
10.1016/j.bbrc.2014.05.059

14. Phosphorylation of sterol regulatory element binding protein-1a by protein kinase A (PKA) regulates transcriptional activity

Author:
Dong, Qingming; Giorgianni, Francesco; Deng, Xiong; Beranova-Giorgianni, Sarka; Bridges, Dave; Park, Edwards A.; Raghow, Rajendra; Elam, Marshall B.
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 449-454
ISSN:
0006-291X
Subject:
binding proteins; cAMP-dependent protein kinase; glucagon; lipogenesis; mammals; mass spectrometry; mutation; phosphorylation; serine; transcription (genetics)
Abstract:
... The counter-regulatory hormone glucagon inhibits lipogenesis via downregulation of sterol regulatory element binding protein 1 (SREBP-1). The effect of glucagon is mediated via protein kinase A (PKA). To determine if SREBP-1 is a direct phosphorylation target of PKA, we conducted mass spectrometry analysis of recombinant n-terminal SREBP-1a following PKA treatment in vitro. This analysis identifie ...
DOI:
10.1016/j.bbrc.2014.05.046
PubMed:
24853806
PubMed Central:
PMC4670028

15. Potential anti-osteoporotic activity of low-molecular weight hyaluronan by attenuation of osteoclast cell differentiation and function in vitro

Author:
Lee, Chang Won; Seo, Jeong Yeon; Choi, Ji Won; Lee, Jisun; Park, Joo Woong; Lee, Jae Yeon; Hwang, Kyo-Yeol; Park, Young Shik; Park, Yong Il
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 438-443
ISSN:
0006-291X
Subject:
Streptococcus equi subsp. zooepidemicus; Western blotting; acid phosphatase; actin; adverse effects; bone resorption; carbonate dehydratase; cathepsin K; cell differentiation; drugs; foods; gelatinase B; genes; hyaluronic acid; in vitro studies; ingredients; integrins; macrophages; mice; osteoclasts; osteoporosis; reverse transcriptase polymerase chain reaction; transcription factor NF-kappa B; tumor necrosis factors; tyrosine
Abstract:
... Due to some severe side effects or lack of efficacy of currently used synthetic drugs, such as bisphosphonates (BPs), the search for new therapeutic agents that can more effectively prevent and treat osteoporosis (OP) has been an increasingly important topic of research. In this study, the low-molecular weight hyaluronan (LMW-HA, 50kDa) produced by enzymatic degradation of high-molecular weight hy ...
DOI:
10.1016/j.bbrc.2014.05.050

16. P66Shc mediates increased platelet activation and aggregation in hypercholesterolemia

Author:
Kumar, Santosh; Vikram, Ajit; Kim, Young-Rae; S. Jacobs, Julia; Irani, Kaikobad
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 496-501
ISSN:
0006-291X
Subject:
agonists; high fat diet; humans; hypercholesterolemia; immunoblotting; mice; oxidative stress; phenotype; platelet activation; quantitative polymerase chain reaction; reactive oxygen species; reverse transcriptase polymerase chain reaction; small interfering RNA; thrombin; transgenic animals
Abstract:
... Hypercholesterolemia leads to a prothrombotic phenotype. Platelet hyperactivity associated with hypercholesterolemia has been attributed, in part, to oxidative stress. P66Shc is a well-known determinant of cellular and organismal oxidative stress. However, its role in platelet biology is not known. We hypothesized that p66Shc mediates platelet hyperactivation and hyperaggregation in hypercholester ...
DOI:
10.1016/j.bbrc.2014.05.029

17. Smooth muscle progenitor cells from peripheral blood promote the neovascularization of endothelial colony-forming cells

Author:
Joo, Hyung Joon; Seo, Ha-Rim; Jeong, Hyo Eun; Choi, Seung-Cheol; Park, Jae Hyung; Yu, Cheol Woong; Hong, Soon Jun; Chung, Seok; Lim, Do-Sun
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 405-411
ISSN:
0006-291X
Subject:
adults; angiogenesis; blood; cell proliferation; coculture; cytokines; endothelial cells; genes; ischemia; mice; models; myocytes; smooth muscle; stem cells; therapeutics; tissue repair
Abstract:
... Proangiogenic cell therapy using autologous progenitors is a promising strategy for treating ischemic disease. Considering that neovascularization is a harmonized cellular process that involves both endothelial cells and vascular smooth muscle cells, peripheral blood-originating endothelial colony-forming cells (ECFCs) and smooth muscle progenitor cells (SMPCs), which are similar to mature endothe ...
DOI:
10.1016/j.bbrc.2014.05.061

18. Structural basis of the phosphorylation dependent complex formation of neurodegenerative disease protein Ataxin-1 and RBM17

Author:
Kim, Eunji; Lee, Yoonji; Choi, Sun; Song, Ji-Joon
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 399-404
ISSN:
0006-291X
Subject:
mutagenesis; neurodegenerative diseases; pathogenesis; phosphorylation; surface plasmon resonance
Abstract:
... Spinocerebellar Ataxia Type1 (SCA1) is a dominantly inherited neurodegenerative disease and belongs to polyglutamine expansion disorders. The polyglutamine expansion in Ataxin-1 (ATXN1) is responsible for SCA1 pathology. ATXN1 forms at least two distinct complexes with Capicua (CIC) or RNA-binding motif protein 17 (RBM17). The wild-type ATXN1 dominantly forms a complex with CIC and the polyglutami ...
DOI:
10.1016/j.bbrc.2014.05.063

19. Structural characterization and interaction of periostin and bone morphogenetic protein for regulation of collagen cross-linking

Author:
Hwang, Eun Young; Jeong, Mi Suk; Park, Eun-Kyeong; Kim, Jae Ho; Jang, Se Bok
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 425-431
ISSN:
0006-291X
Subject:
Rhodobacter sphaeroides; binding sites; bone morphogenetic proteins; collagen; crosslinking; extracellular matrix; fibroblasts; fibronectins; fibrosis; heart; insects; neoplasm cells; nuclear magnetic resonance spectroscopy; protein secretion; transforming growth factor beta
Abstract:
... Periostin appears to be a unique extracellular protein secreted by fibroblasts that is upregulated following injury to the heart or changes in the environment. Periostin has the ability to associate with other critical extracellular matrix (ECM) regulators such as TGF-β, tenascin, and fibronectin, and is a critical regulator of fibrosis that functions by altering the deposition and attachment of c ...
DOI:
10.1016/j.bbrc.2014.05.055

20. Upregulation of tumor necrosis factor-alpha in nucleus accumbens attenuates morphine-induced rewarding in a neuropathic pain model

Author:
Wu, Ying; Na, Xiaodong; Zang, Ying; Cui, Yu; Xin, Wenjun; Pang, Ruiping; Zhou, Lijun; Wei, Xuhong; Li, Yongyong; Liu, Xianguo
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 502-507
ISSN:
0006-291X
Subject:
biosynthesis; dopamine; gene expression regulation; mice; models; morphine; narcotics; nerve tissue; pain; rats; risk; tumor necrosis factor-alpha; tyrosine 3-monooxygenase
Abstract:
... Treatment of neuropathic pain with opioid analgesics remains controversial and a major concern is the risk of addiction. Here, we investigated this issue with spared nerve injury (SNI) model of neuropathic pain in rats and mice. SNI prevented conditioned place preference (CPP) induced by low dose (3.5mg/kg) of morphine (MOR), which was effective for anti-allodynia, but not by high dose (⩾5.0mg/kg) ...
DOI:
10.1016/j.bbrc.2014.05.025