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You searched for: Journal name Biochemical and biophysical research communications Remove constraint Journal name: Biochemical and biophysical research communications
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1. (+)-Sesamin, a sesame lignan, is a potent inhibitor of gut bacterial tryptophan indole-lyase that is a key enzyme in chronic kidney disease pathogenesis

Author:
Daiki Oikawa; Satoshi Yamashita; Seiji Takahashi; Toshiyuki Waki; Koichi Kikuchi; Takaaki Abe; Takane Katayama; Toru Nakayama
Source:
Biochemical and biophysical research communications 2022 v.590 pp. 158-162
ISSN:
0006-291X
Subject:
Escherichia coli; active sites; dietary supplements; enzymes; intestines; kidney diseases; kidneys; lignans; mice; morbidity; pathogenesis; phytochemicals; pyridoxal; research; sulfates; therapeutics; toxins; tryptophan
Abstract:
... The progression of chronic kidney disease (CKD) increases the risks of cardiovascular morbidity and end-stage kidney disease. Indoxyl sulfate (IS), which is derived from dietary l-tryptophan by the action of bacterial l-tryptophan indole-lyase (TIL) in the gut, serves as a uremic toxin that exacerbates CKD-related kidney disorder. A mouse model previously showed that inhibition of TIL by 2-aza-l-t ...
DOI:
10.1016/j.bbrc.2021.12.088

2. 1-, 2- and 3-AG as substrates of the endocannabinoid enzymes and endogenous ligands of the cannabinoid receptor 1

Author:
Shrouq I. Farah; Samantha Hilston; Ngan Tran; Nikolai Zvonok; Alexandros Makriyannis
Source:
Biochemical and biophysical research communications 2022 v.591 pp. 31-36
ISSN:
0006-291X
Subject:
acylglycerol lipase; agonists; amide hydrolases; cannabinoid receptors; cannabinoids; enantiomers; fatty acids; hydrolysis; isomerization; ligands; monoacylglycerols; research; serine; stereochemistry
Abstract:
... 2-Arachidonoylglycerol (2-AG) is the most potent and abundant endocannabinoid that acts as a full agonist at the cannabinoid 1 (CB1) and 2 (CB2) receptors. It serves as a substrate for several serine hydrolases, including monoacylglycerol lipase (MGL), α/β hydrolase domain 6 (ABHD6) and fatty acid amide hydrolase (FAAH). However, 2-AG's rapid conversion to 1-AG (the S stereoisomer) and 3-AG (the R ...
DOI:
10.1016/j.bbrc.2021.12.105

3. 3-Hydroxyacyl-CoA dehydratase 2 deficiency confers resistance to diet-induced obesity and glucose intolerance

Author:
Lengyun Wei; Shengmei Weng; Xuyang Lu; Shenglong Zhu; Qin Yang; Yong Q. Chen
Source:
Biochemical and biophysical research communications 2022 v.605 pp. 134-140
ISSN:
0006-291X
Subject:
diabetes; energy expenditure; enzymes; fatty acids; fatty liver; glucose; liver; obesity; pandemic; research; therapeutics
Abstract:
... Obesity and associated complications are becoming a pandemic. Inhibiting fatty acid synthesis and elongation is an important intervention for the treatment of obesity. Despite intensive investigations, many potential therapeutic targets have yet to be discovered. In this study, decreased expression of Hacd2 (a newly found enzyme in fatty acid elongation) was found in HFD induced mice and loss of H ...
DOI:
10.1016/j.bbrc.2022.03.057

4. 3D-cultured small size adipose-derived stem cell spheroids promote bone regeneration in the critical-sized bone defect rat model

Author:
Yutaro Yamada; Tadashi Okano; Kumi Orita; Tomomi Makino; Fumiaki Shima; Hiroaki Nakamura
Source:
Biochemical and biophysical research communications 2022 v.603 pp. 57-62
ISSN:
0006-291X
Subject:
angiogenesis; animal models; apoptosis; cartilage; gene expression; immunohistochemistry; morphogenesis; research; skull; stem cells; therapeutics
Abstract:
... Adipose-derived stem cells (ADSCs), due to their regenerative ability, have beneficial effects on bone and cartilage defects. In addition, spheroid formation of ADSCs obtained using three-dimensional (3D) culture accelerates the regenerative ability of ADSCs. The study investigated the regenerative effect of 3D-cultured small size ADSC spheroids without a scaffold in rats with defects in the criti ...
DOI:
10.1016/j.bbrc.2022.03.027

5. A BTB/POZ domain-containing protein negatively regulates plant immunity in Nicotiana benthamiana

Author:
Mengwei Zhao; Yu Ge; Zhangyan Xu; Xue Ouyang; Yuling Jia; Jiangtao Liu; Meixiang Zhang; Yuyan An
Source:
Biochemical and biophysical research communications 2022 v.600 pp. 54-59
ISSN:
0006-291X
Subject:
Nicotiana benthamiana; Phytophthora nicotianae var. parasitica; Pseudomonas syringae; Ralstonia solanacearum; hypersensitive response; immunity; pathogens; research; ubiquitin; ubiquitin-protein ligase
Abstract:
... Plants have evolved immune systems to fight against pathogens. However, it is still largely unknown how the plant immunity is finely regulated. Here we identified a BTB/POZ domain-containing protein, namely NbBTB, which is predicted to be a member of the ubiquitin E3 ligase complex. The NbBTB expression is downregulated upon the oomycete pathogen Phytophthora parasitica infection. Overexpression o ...
DOI:
10.1016/j.bbrc.2022.02.050

6. A chimeric switch-receptor PD1-DAP10-41BB augments NK92-cell activation and killing for human lung Cancer H1299 Cell

Author:
Lingtong Zhi; Meichen Yin; Xin Su; Zikang Zhang; Hui Lu; Mingfeng Li; Changjiang Guo; Zhiyuan Niu; Xuan Zhang; Wuling Zhu
Source:
Biochemical and biophysical research communications 2022 v.600 pp. 94-100
ISSN:
0006-291X
Subject:
apoptosis; cytotoxicity; humans; immunotherapy; lung neoplasms; neoplasm cells; phenotype; research; signal transduction
Abstract:
... Engineered natural killer (NK) cell-based therapies have been potentially broadly applicable and exhibited promising results in clinical trials, particularly in the fight against cancers. NK cell immunotherapy however always remains variable. One major obstacle is the inhibitory pathway including PD1/PDL1, providing tumor cells an escape mechanism from immunosurveillance. In this regard, we ration ...
DOI:
10.1016/j.bbrc.2022.02.052

7. A co-expression vector for baculovirus-mediated protein expression in mammalian cells

Author:
Wenjun Guo; Mengmeng Wang; Lei Chen
Source:
Biochemical and biophysical research communications 2022 v.594 pp. 69-73
ISSN:
0006-291X
Subject:
humans; insects; protein synthesis; research; toxicity; viruses
Abstract:
... BacMam system utilizes baculovirus to deliver exogenous genes into mammalian cells and is extensively used for recombinant production of eukaryotic proteins. Here, we described the development of a BacMam vector (pBMCL1), which allows convenient tracing of virus production, provides higher infection efficiency towards mammalian cells, minimizes unwanted transcription of toxic genes in insect cells ...
DOI:
10.1016/j.bbrc.2022.01.056

8. A conserved asparagine residue in the inner surface of BRI1 superhelix is essential for protein native conformation

Author:
Huchen Zhang; Shijie Yi; Yuan Zhang; Zhi Hong
Source:
Biochemical and biophysical research communications 2022 pp. -
ISSN:
0006-291X
Subject:
asparagine; brassinosteroids; eukaryotic cells; glycosylation; hydrogen bonding; mutants; mutation; plasma membrane; research; sequence analysis
Abstract:
... Asparagine-linked glycosylation (ALG, N-glycosylation) is one of the most prevalent protein modifications in eukaryotes and regulates protein folding, trafficking and function. Recently, we reported that the mutation of N154Q significantly led to the ER retention of brassinosteroids insensitive 1 (BRI1), the receptor of brassinosteroids (BRs). However, the mechanism of how the N154 site affects BR ...
DOI:
10.1016/j.bbrc.2022.05.014

9. A gain-of-function mutation in the acid-sensing ion channel 2a induces marked cerebellar maldevelopment in rats

Author:
Yasuhiro Shibata; Natsuko Kumamoto; Eisuke Sakuma; Yusuke Ishida; Takashi Ueda; Shoichi Shimada; Shinya Ugawa
Source:
Biochemical and biophysical research communications 2022 v.610 pp. 77-84
ISSN:
0006-291X
Subject:
adults; amino acids; atrophy; cerebellum; cytotoxicity; gain-of-function mutation; gait; gene expression; genes; genetically modified organisms; heterozygosity; histology; ion channels; research
Abstract:
... Specific amino acid substitutions in degenerin mechano-gated channels (DEGs) of C. elegans convert these channels into constitutively active mutants that induce the degeneration of neurons where DEGs are expressed. Acid-sensing ion channel-2a (ASIC2a), a proton-gated cation channel predominantly expressed in central neurons, is a mammalian ortholog of DEGs, and it can remain unclosed to be cytotox ...
DOI:
10.1016/j.bbrc.2022.04.030

10. A high efficient FVIII variant corrects bleeding in hemophilia A mouse model

Author:
Dhahiri Saidi Mashausi; Debmalya Roy; Hitesh Bhagavanbhai Mangukiya; Siva Bharath Merugu; Ghulam Raza; Fakhar-Un-Nisa Yunus; Guo-Song Liu; Hema Negi; Dawei Li
Source:
Biochemical and biophysical research communications 2022 pp. -
ISSN:
0006-291X
Subject:
coagulants; coagulation; gene therapy; gene transfer; hemophilia; histology; hydrodynamics; liver; mice; point mutation; research; site-directed mutagenesis; toxicity
Abstract:
... Hemophilia A is a bleeding disorder caused by quantitative or qualitative deficiencies in coagulation factor VIII (FVIII). Low FVIII expression due to its unstable mRNA and binding with immunoglobulin-binding protein (BiP) compromises gene therapy endeavors in hemophilia A. Site-directed mutagenesis have demonstrated an improvement in the expression of FVIII proteins. In this study, a targeted poi ...
DOI:
10.1016/j.bbrc.2022.02.066

11. A high-affinity aptamer with base-appended base-modified DNA bound to isolated authentic SARS-CoV-2 strains wild-type and B.1.617.2 (delta variant)

Author:
Hirotaka Minagawa; Hirofumi Sawa; Tomoko Fujita; Shintaro Kato; Asumi Inaguma; Miwako Hirose; Yasuko Orba; Michihito Sasaki; Koshiro Tabata; Naoki Nomura; Masashi Shingai; Yasuhiko Suzuki; Katsunori Horii
Source:
Biochemical and biophysical research communications 2022 pp. -
ISSN:
0006-291X
Subject:
DNA; DNA libraries; Severe acute respiratory syndrome coronavirus 2; antibodies; dissociation; glycoproteins; guanine; oligonucleotides; research; systematic evolution of ligands by exponential enrichment; uracil
Abstract:
... Simple, highly sensitive detection technologies for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are crucial for the effective implementation of public health policies. We used the systematic evolution of ligands by exponential enrichment with a modified DNA library, including a base-appended base (uracil with a guanine base at its fifth position), to create an aptamer with a high ...
DOI:
10.1016/j.bbrc.2022.04.071

12. A molecular mechanism of UDCA engagement with GPBAR and subsequent G protein interaction revealed by scattered alanine scanning

Author:
Ruirui Lu; Xu Yan; Guoxing Fang; Yuming Zhuang; Lulu Guo; Chao Zhang; Xiang Wu; Peng Xiao; Yiwen Cao; Fan Yang; Xiao Yu; Jin-peng Sun; Jiu-yao Zhou
Source:
Biochemical and biophysical research communications 2022 v.600 pp. 14-21
ISSN:
0006-291X
Subject:
Ursidae; alanine; bile; cholesterol; drugs; metabolism; oxygen; research; therapeutics; ursodeoxycholic acid
Abstract:
... As the most known therapeutic component of bear bile acids, ursodeoxycholic acid (UDCA) is an FDA-approved drug for the treatment of primary biliary cirrhosis (PBC), the dissolution of cholesterol gallstones. UDCA produces many beneficial effects on metabolism and immune responses via its interaction with the membrane G protein-coupled bile acid receptor (GPBAR); however, how UDCA interacts with G ...
DOI:
10.1016/j.bbrc.2022.02.030

13. A mouse model of weight gain after nicotine withdrawal

Author:
Katsushi Takeda; Daisuke Aotani; Yusuke Kuga; Tomoki Jinno; Tingting Guo; Kento Ogawa; Yuki Shimizu; Rei Hattori; Takashi Yagi; Hiroyuki Koyama; Shigenobu Matsumura; Hiromi Kataoka; Tomohiro Tanaka
Source:
Biochemical and biophysical research communications 2022 v.588 pp. 140-146
ISSN:
0006-291X
Subject:
appetite; body weight changes; energy intake; mice; nicotine; oxygen consumption; peptides; research; weight gain
Abstract:
... Smoking cessation increases body weight. The underlying mechanisms, however, have not been fully understood. We here report an establishment of a mouse model that exhibits an augmented body weight gain after nicotine withdrawal. High fat diet-fed mice were infused with nicotine for two weeks, and then with vehicle for another two weeks using osmotic minipumps. Body weight increased immediately aft ...
DOI:
10.1016/j.bbrc.2021.12.064

14. A new method for primary culture of microglia in rats with spinal cord injury

Author:
Chengjie Wu; Yalan Pan; Lining Wang; Mengmin Liu; Mao Wu; Jianwei Wang; Guanglu Yang; Yang Guo; Yong Ma
Source:
Biochemical and biophysical research communications 2022 v.599 pp. 63-68
ISSN:
0006-291X
Subject:
animal injuries; fluorescent antibody technique; models; neuroglia; research; spinal cord
Abstract:
... At present, the primary culture method of microglia is complicated, and the culture of spinal cord microglia is rare, so we will explore to establish a new and efficient primary culture method of microglia in rats with spinal cord injury (SCI). The SCI model of SD rats was established by modified A11en′s method, and the model of SCI was performed on 1 d, 3 d, 7 d and 14 d respectively. Then the in ...
DOI:
10.1016/j.bbrc.2022.02.027

15. A new network containing MYB109-ZmCesA5 is involved in kernel development

Author:
Erfei Dong; Chaoxian Liu; Yang Bai; Xiupeng Mei; Zikun Zhao; Siying Zhu; Jiahao Zhang; Yilin Cai
Source:
Biochemical and biophysical research communications 2022 v.602 pp. 15-20
ISSN:
0006-291X
Subject:
corn; metabolism; quantitative polymerase chain reaction; research; seed development; starch; sucrose; transcriptome; weight loss; yeasts
Abstract:
... MYB genes regulate several different aspects of metabolism and development. However, few studies have reported the involvement of MYBs-CesAs network in the regulation of maize kernel development. In this study, yeast one-hybrid (Y1H) assays and dual-luciferase reporter assays showed that ZmMYB109 activated the expression of ZmCesA5 by directly binding to its promoter. Real-time quantitative PCR (R ...
DOI:
10.1016/j.bbrc.2022.02.104

16. A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid

Author:
Di Wu; Pierre-André Gilormini; Sakura Toda; Christophe Biot; Cédric Lion; Yann Guérardel; Chihiro Sato; Ken Kitajima
Source:
Biochemical and biophysical research communications 2022 pp. -
ISSN:
0006-291X
Subject:
Oncorhynchus mykiss; catalytic activity; enzyme activity; enzymes; glycoconjugates; metabolism; mice; research; sialic acid
Abstract:
... The CMP-sialic acid synthetase (CSS) activates free sialic acid (Sia) to CMP-Sia using CTP, and is prerequisite for the sialylation of cell surface glycoconjugates. The vertebrate CSS consists of two domains, a catalytic N-domain and a non-catalytic C-domain. Although the C-domain is not required for the CSS enzyme to synthesize CMP-Sia, its involvement in the catalytic activity remains unknown. F ...
DOI:
10.1016/j.bbrc.2022.05.031

17. A novel PDK1 inhibitor, JX06, inhibits glycolysis and induces apoptosis in multiple myeloma cells

Author:
Yawara Kawano; Takayuki Sasano; Yuichiro Arima; Saki Kushima; Kenichi Tsujita; Masao Matsuoka; Hiroyuki Hata
Source:
Biochemical and biophysical research communications 2022 v.587 pp. 153-159
ISSN:
0006-291X
Subject:
apoptosis; cell growth; data collection; gene expression; glucose; glycolysis; mechanism of action; metabolites; mitochondria; myeloma; oxidation; phosphorylation; proteasome endopeptidase complex; proteasome inhibitors; pyruvate dehydrogenase (acetyl-transferring) kinase; pyruvate dehydrogenase (lipoamide); research; therapeutics
Abstract:
... Pyruvate dehydrogenase kinase 1 (PDK1) is a Ser/Thr kinase that inactivates mitochondrial pyruvate dehydrogenase (PDH), leading to switch of glucose metabolism from mitochondrial oxidation to aerobic glycolysis. We previously reported that PDK1 inhibition is a potent therapeutic strategy in multiple myeloma (MM). However, availability of PDK1 inhibitors, which are effective at low concentrations, ...
DOI:
10.1016/j.bbrc.2021.11.102

18. A novel carotenoid biosynthetic route via oxidosqualene

Author:
Yusuke Otani; Takashi Maoka; Shigeko Kawai-Noma; Kyoichi Saito; Daisuke Umeno
Source:
Biochemical and biophysical research communications 2022 v.599 pp. 75-80
ISSN:
0006-291X
Subject:
Methylomonas; Staphylococcus aureus; biochemical pathways; biosynthesis; carotenoids; research; triterpenoids
Abstract:
... Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjuga ...
DOI:
10.1016/j.bbrc.2022.01.105

19. A novel insight into the molecular mechanism of human soluble guanylyl cyclase focused on catalytic domain in living cells

Author:
Jiannan Li; Yajun Zhou; Ying-Wu Lin; Xiangshi Tan
Source:
Biochemical and biophysical research communications 2022 v.604 pp. 51-56
ISSN:
0006-291X
Subject:
active sites; fluorescence; guanylate cyclase; humans; hydrogen; metalloproteins; mutagenesis; mutants; research
Abstract:
... Human soluble guanylate cyclase (sGC) is a heme-containing metalloprotein in NO-sGC-cGMP signaling. In this work, fluorescent proteins were employed to study the NO-induced sGC molecular mechanism via mutagenesis at the catalytic domain. The conformational change of sGC by mutant α₁C595 was investigated in living cells through fluorescence lifetime imaging microscopy (FLIM). The results indicated ...
DOI:
10.1016/j.bbrc.2022.03.023

20. A novel isoform of hydroxyacyl-CoA dehydrogenase inhibits cell proliferation

Author:
Guiyu Xia; Yunyi Gao; Canlan Wu; Haitao Pan; Jingyu Hou; Jinfeng Su; Saisai Wei; Xiangwei Gao
Source:
Biochemical and biophysical research communications 2022 v.606 pp. 75-79
ISSN:
0006-291X
Subject:
cell proliferation; fibroblasts; mice; mitochondria; oxidoreductases; research; start codon
Abstract:
... Hydroxyacyl-CoA dehydrogenase (HADH) catalyzes the third reaction of mitochondrial β-oxidation cascade, while the regulation of its expression and function remains to be elucidated. Using the quantitative translation initiation sequencing (QTI-seq), we have identified that murine Hadh mRNA has two alternative translation start codons. We demonstrated that translation from upstream start codon enco ...
DOI:
10.1016/j.bbrc.2022.03.102