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- Futaki, Shiroh, et al. Show all 3 Authors
- Biochemical and biophysical research communications 2013 v.441 pp. 262-265
- DNA; binding sites; evolution; genetic engineering; mutants; nucleotide sequences; point mutation; transcription (genetics); tryptophan
- ... Transcription activator-like effectors (TALEs) are convenient tools for genome engineering at specific genomic sites. However, their use is constrained because most TALE binding sites are preceded by a highly conserved 5′ terminal T nucleotide (5′-T). To remove the 5′-T constraint, we substituted tryptophan 232 in the repeat-1 loop region of the dHax3 N-terminal domain for other amino acids. Furth ...
- Futaki, Shiroh, et al. Show all 5 Authors
- Biochemical and biophysical research communications 2014 v.446 pp. 857-862
- binding proteins; peptides; plasma membrane; protein kinase C
- ... Arginine-rich cell-penetrating peptides (CPPs) are promising carriers for the intracellular delivery of various bioactive molecules. However, many ambiguities remain about the molecular interplays on cell surfaces that ultimately lead to endocytic uptake of CPPs. By treatment of cells with octaarginine (R8), enhanced clustering of syndecan-4 on plasma membranes and binding of protein kinase Cα (PK ...