U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal name Biochemical engineering journal Remove constraint Journal name: Biochemical engineering journal Publication year rev 7978-2022 Remove constraint Publication year rev: 7978-2022 Source 2022 v.181 Remove constraint Source: 2022 v.181
Start Over

Search

28  Search Results

« Previous | 1 - 20 of 28 |
Number of results to display per page

Search Results

1. Alkaline pretreatment of Taraxacum kok-saghyz (TK) roots for the extraction of natural rubber (NR)

Author:
David A. Ramirez Cadavid; Upul Hathwaik; Katrina Cornish; Colleen McMahan; Frederick C. Michel
Source:
Biochemical engineering journal 2022 v.181 pp. 108376
ISSN:
1369-703X
Subject:
Taraxacum kok-saghyz; alkali treatment; biocompatible materials; endo-1,4-beta-glucanase; enzymatic treatment; gels; inulin; molecular weight; polygalacturonase; rubber; sodium hydroxide; temperature
Abstract:
... Natural rubber (NR) is an essential biomaterial the supply of which is insecure. Taraxacum kok-saghyz (TK), a rubber-producing dandelion, is an alternative source of NR that has excellent potential for commercial development. Scalable methods for rubber extraction at high yield, quality and purity are needed to meet this potential. We previously developed an aqueous, enzymatic method for the extra ...
DOI:
10.1016/j.bej.2022.108376

2. CircRNA hsa_circ_0001421 promotes the osteoblast differentiation of human adipose mesenchymal stem cells through the miR-608/SP7 axis

Author:
Qiting He; Hengshuo Zhang; Beibei Hu; Xuezhou Li; Yunpeng Wu; Pengfei Sun; Yuhua Jia; Lei Cheng; Yongyuan Guo
Source:
Biochemical engineering journal 2022 v.181 pp. 108386
ISSN:
1369-703X
Subject:
alizarin; alkaline phosphatase; bone density; bone formation; circular RNA; humans; luciferase; osteoblasts; osteoporosis; reporter genes
Abstract:
... Human adipose mesenchymal stem cells (hAMSCs) can differentiate into osteoblasts, which can enhance bone mass and bone formation. Circular RNA plays an important role in the osteoblast differentiation of mesenchymal stem cells, but the mechanism remains unclear. We used hAMSCs to study the role of circRNA hsa_circ_0001421/miR-608/SP7 axis in osteoblast differentiation. qRT-PCR, western blotting, a ...
DOI:
10.1016/j.bej.2022.108386

3. Deodorization of the off-gas from livestock manure aerobic composting tank using biotrickling filters and its mechanism

Author:
Longhua Deng; Jiaxin Xu; Yuxin Li; Shangquan Wu; Kecheng Yi; Ruizhi Dai; Songgui He; Yanbin Xu
Source:
Biochemical engineering journal 2022 v.181 pp. 108409
ISSN:
1369-703X
Subject:
Ralstonia; ammonia; animal manures; biofilm; denitrification; deodorization; nitrification; odors; oxygen; pH; volatile organic compounds; volcanic rocks
Abstract:
... In this study, two biotrickling filters (BTFs) were fabricated to treat the off-gas produced during the aerobic composting of animal manure, namely: BTF-A (packed with volcanic rock) and BTF-B (packed with ceramsite). The results showed that the major contributors to the odor level of the off-gas were ammonia (NH₃) and total volatile organic compounds (TVOCs). The removal efficiencies of BTF-B for ...
DOI:
10.1016/j.bej.2022.108409

4. Design of enzymatic cascade reactors through multi-objective dynamic optimization

Author:
Leandros Paschalidis; Barbara Beer; Samuel Sutiono; Volker Sieber; Jakob Burger
Source:
Biochemical engineering journal 2022 v.181 pp. 108384
ISSN:
1369-703X
Subject:
batch systems; decision making; engineering; engineers; enzymes; journals; space and time
Abstract:
... Multi-enzymatic cascade reactions have gathered a lot of interest lately because of their potential for sustainable production of chemicals. In this paper, model-based, multi-objective, dynamic optimization is applied to the design of an enzymatic cascade reactor that produces α-ketoglutarate. Pareto-optimal process schedules, which are best compromises between space-time yield, enzyme consumption ...
DOI:
10.1016/j.bej.2022.108384

5. Heterotrophic properties of Myrmecia incisa and strategies for enhanced arachidonic acid production

Author:
Jia-Xing Guo; Zhi-Gang Zhou; Carol Sze Ki Lin; Zheng Sun
Source:
Biochemical engineering journal 2022 v.181 pp. 108399
ISSN:
1369-703X
Subject:
Lobosphaera incisa; arachidonic acid; biomass production; dissolved oxygen; feedstocks; glucose; human health; inoculum; microalgae; nutrient deficiencies; oils; pH
Abstract:
... Myrmecia incisa is a valuable microalga worth investigating as it is rich in arachidonic acid (ArA), a polyunsaturated fatty acid that is essential for human health. The present study developed a two-stage cultural strategy for M. incisa. In stage Ⅰ, heterotrophic mode was conducted for the purpose of high algal biomass production. By determining the optimal cultural parameters including dissolved ...
DOI:
10.1016/j.bej.2022.108399

6. In-silico screening of potential target transporters for glycyrrhetinic acid (GA) via deep learning prediction of drug-target interactions

Author:
Adham M. Alkhadrawi; Ying Wang; Chun Li
Source:
Biochemical engineering journal 2022 v.181 pp. 108375
ISSN:
1369-703X
Subject:
ABC transporters; Glycyrrhiza; computer simulation; drugs; glycyrrhetinic acid; licorice; prediction; probability
Abstract:
... Glycyrrhetinic acid (GA) is one of the major bioactive components of the leguminous plant Glycyrrhiza spp. (Chinese licorice). The mechanism by which GA is transported within or out of cells remains completely unknown. Given the fact that living organisms harbor thousands of membrane transporters, experimental verification of membrane transporters for specific GA transporters is both laborious and ...
DOI:
10.1016/j.bej.2022.108375

7. Influence of CPM-dependent sorting on the multi-omics profile of hepatocyte-like cells matured in microscale biochips

Author:
Mathieu Danoy; Stéphane Poulain; Benedikt Scheidecker; Rachid Jellali; Yannick Tauran; Marjorie Leduc; Johanna Bruce; Francoise Gilard; Bertrand Gakiere; Hiroshi Arakawa; Yukio Kato; Soo Hyeon Kim; Taketomo Kido; Atsushi Miyajima; Yasuyuki Sakai; Eric Leclerc
Source:
Biochemical engineering journal 2022 v.181 pp. 108408
ISSN:
1369-703X
Subject:
RNA; drugs; humans; liver; liver regeneration; metabolomics; multiomics; proteomics; reproduction
Abstract:
... Liver modeling in disease via advanced in vitro physiological tissues remains a challenging issue, yet crucial for the future development of relevant tools for drug screening. In that regard, advanced technics such as human induced Pluripotent Stem Cells (hiPSCs) and organ-on-chip are promising technologies for the relevant reproduction of the in vivo micro physiology in in vitro culture condition ...
DOI:
10.1016/j.bej.2022.108408

8. Preparation optimization and stability comparison study of alkali-modified biochar immobilized laccase under multi-immobilization methods

Author:
Zhaobo Wang; Dajun Ren; Hongyan Yu; Shuqin Zhang; Xiaoqing Zhang; Wangsheng Chen
Source:
Biochemical engineering journal 2022 v.181 pp. 108401
ISSN:
1369-703X
Subject:
adsorption; biochar; immobilized enzymes; laccase; pH; temperature
Abstract:
... Alkali modified biochar has attracted many interests as a potential enzyme immobilization carrier. In this paper, the preparation of alkali-modified immobilized laccases under multi-immobilization methods (adsorption: A/lac@A-MB; adsorption-crosslinking: A-C/lac@A-MB; covalent bonding: CB/lac@A-MB) were optimized via Design-Expert for the first time, and the stability of alkali-modified immobilize ...
DOI:
10.1016/j.bej.2022.108401

9. Promoting interspecies hydrogen/electron transfer in Bio-PdNPs-mediated denitrification with the selectivity towards N2

Author:
Sijing Bi; Huimin Liu; Yuancai Chen; Yongyou Hu
Source:
Biochemical engineering journal 2022 v.181 pp. 108395
ISSN:
1369-703X
Subject:
biosynthesis; denitrification; electron transfer; fermentation; hydrogen; palladium; riboflavin; sludge
Abstract:
... The palladium nanoparticles (Bio-PdNPs) were in-situ biosynthesized in denitrifying sludge at three Pd loading levels, and the denitrification performance was evaluated in terms of interspecies hydrogen/electron transfer (IHT/IET) and their pathway for the first time. The results showed that high selectivity towards N₂ (76.96%, 97.10% and 99.30%) were observed when conventional denitrifying sludge ...
DOI:
10.1016/j.bej.2022.108395

10. Purification and characterisation of (S)-specific alcohol dehydrogenase from Candida parapsilosis ATCC 7330

Author:
Jerrina Pinto; Anju Chadha; Sathyanarayana N. Gummadi
Source:
Biochemical engineering journal 2022 v.181 pp. 108406
ISSN:
1369-703X
Subject:
Candida parapsilosis; acetophenones; active sites; alcohol dehydrogenase; biocatalysts; catalytic activity; enantiomers; enantioselectivity; molecular weight; odors; oxidation; pH; zinc
Abstract:
... Alcohol dehydrogenases (ADHs) catalyses reversible reduction of carbonyl group to its corresponding alcohols and have been widely employed as versatile biocatalyst due to its high enantioselectivity to produce chiral alcohols. In this study, the (S) - specific alcohol dehydrogenase (S-ADH) enzyme was purified from Candida parapsilosis ATCC 7330. It asymmetrically reduced acetophenone to (S)-1-phen ...
DOI:
10.1016/j.bej.2022.108406

11. Simultaneous and selective production of exopolymers and polyols by metabolically engineered Bacillus licheniformis strains

Author:
Chan Woo Song; Mina Kwon; Hyohak Song
Source:
Biochemical engineering journal 2022 v.181 pp. 108381
ISSN:
1369-703X
Subject:
Bacillus licheniformis; batch fermentation; carbon; exopolysaccharides; genes; glycerol; levan; metabolic engineering; polyglutamic acid; temperature
Abstract:
... Bacillus licheniformis has tremendous potential for the production of exopolymers that include polyglutamic acid (PGA), levan and exopolysaccharide (EPS); and polyols among which are glycerol and 2,3-butanediol (BDO) isomers. For simultaneous and selective production of exopolymers and polyols, cultivation/fermentation, metabolic engineering, and separation technologies were combined and evaluated ...
DOI:
10.1016/j.bej.2022.108381

12. Small-sized salt-tolerant denitrifying and phosphorus removal aerobic granular sludge cultivated with mariculture waste solids to treat synthetic mariculture wastewater

Author:
Cuiya Zhang; Fan Gao; Yinghai Wu; Guangjing Xu; Haiyan Liu; Hanmin Zhang; Fenglin Yang; Yongping Xu
Source:
Biochemical engineering journal 2022 v.181 pp. 108396
ISSN:
1369-703X
Subject:
batch systems; denitrification; denitrifying microorganisms; glycogen; mariculture; nitrogen; phosphates; phosphorus; salt tolerance; sludge; wastewater
Abstract:
... The mariculture waste solids generated in mariculture system were harmlessly reused to cultivate salt-tolerant aerobic granular sludge to further treat synthetic mariculture wastewater. Salt-tolerant aerobic granules with small size (~350 µm) were obtained after 45 days in an anaerobic/aerobic/anoxic sequencing batch reactor. The functional microorganisms were efficiently enriched as high nitrogen ...
DOI:
10.1016/j.bej.2022.108396

13. Woven-fiber microfiltration coupled with anaerobic forward osmosis membrane bioreactor treating textile wastewater: Use of fertilizer draw solutes for direct fertigation

Author:
Kamran Manzoor; Sher Jamal Khan; Aamir Khan; Hassam Abbasi; Waqas Qamar Zaman
Source:
Biochemical engineering journal 2022 v.181 pp. 108385
ISSN:
1369-703X
Subject:
ammonium sulfate; chemical oxygen demand; color; fertigation; fertilizers; gas production (biological); membrane bioreactors; microfiltration; osmosis; phosphates; salinity; solutes; textile mill effluents; total phosphorus; water reuse
Abstract:
... A lab-scale Anaerobic Forward Osmosis Membrane Bioreactor (An-FOMBR) system was established for treating synthetic textile wastewater having Chemical Oxygen Demand (COD) of 3000 ± 150 mg/L using Fertilizer Draw Solutes (FDSs). The study was conducted by using Ammonium Sulphate (SOA), Mono-ammonium Phosphate (MAP), and Mono-potassium Phosphate (MKP) each having 1 Molar (M) concentration which can b ...
DOI:
10.1016/j.bej.2022.108385

14. Biosurfactants: Production, properties, applications, trends, and general perspectives

Author:
Leonie A. Sarubbo; Maria da Gloria C. Silva; Italo José B. Durval; Káren Gercyane O. Bezerra; Beatriz G. Ribeiro; Ivison A. Silva; Matthew S. Twigg; Ibrahim M. Banat
Source:
Biochemical engineering journal 2022 v.181 pp. 108377
ISSN:
1369-703X
Subject:
biosurfactants; biotechnology; emulsifying; environmental sustainability; foams; hydrophilicity; hydrophobicity; oils; petroleum; public health
Abstract:
... Biosurfactants have become attractive microbial products in the emerging biotechnology industry due to their advantages over synthetic surfactants in terms of environmental sustainability, global public health, and the concerns of industries to produce environmentally friendly goods. The amphipathic structure of biosurfactants with hydrophilic and hydrophobic moieties enable these molecules to pla ...
DOI:
10.1016/j.bej.2022.108377

15. Characterization of packed-bed in the downcomer of a concentric internal-loop airlift bioreactor

Author:
Vítor Teixeira Mazziero; Vinícius Guerso Batista; Daniele Gonçalves de Oliveira; Mateus Scontri; Ariela Veloso de Paula; Marcel Otavio Cerri
Source:
Biochemical engineering journal 2022 v.181 pp. 108407
ISSN:
1369-703X
Subject:
air flow; bioreactors; corn cobs; dyes; enzymes; liquids; models; particle size; permeability; tracer techniques; viscosity
Abstract:
... A packed-bed basket containing corn cob powder was used for the adaptation of a concentric-tube airlift reactor for enzymatic reactions. The bed was inserted in the downcomer region of the bioreactor generating a decrease in liquid downflow. To characterize the liquid velocity through the packed-bed and calculate the residence time, the intrinsic permeability was determined using Darcy’s law, resu ...
DOI:
10.1016/j.bej.2022.108407

16. Diverting mevalonate pathway metabolic flux leakage in Saccharomyces cerevisiae for monoterpene geraniol production from cane molasses

Author:
Yan Guo; Fuxiao Li; Jingyi Zhao; Xin Wei; Zhiqi Wang; Jidong Liu
Source:
Biochemical engineering journal 2022 v.181 pp. 108398
ISSN:
1369-703X
Subject:
Saccharomyces cerevisiae; biofuels; biosynthesis; carbon; fermentation; geraniol; molasses; odors; phosphates; yeasts
Abstract:
... Geraniol has versatile applications in the fragrance, pharmacy, and biofuel industries. This monoterpene is produced via the mevalonate pathway in the yeast Saccharomyces cerevisiae. However, carbon flux leakage restricts the efficient biosynthesis of target compounds via this pathway. In this work, the mevalonate pathway of the recombinant S. cerevisiae was reprogrammed by introducing an isopente ...
DOI:
10.1016/j.bej.2022.108398

17. Enhancing the biomass and riboflavin production of Ashbya gossypii by using low-intensity ultrasound stimulation

Author:
Jiantang Chen; Wenjuan Zhai; Yuan Li; Yan Guo; Yuan Zhu; Guanghong Lei; Jianbin Li
Source:
Biochemical engineering journal 2022 v.181 pp. 108394
ISSN:
1369-703X
Subject:
Aphis gossypii; Eremothecium gossypii; biomass; fermentation; fungi; mass transfer; membrane permeability; mycelium; reactive oxygen species; riboflavin; ultrasonics
Abstract:
... The riboflavin (vitamin B₂) produced by the fermentation of Ashbya gossypii is an essential nutrient for humans and animals. In this study, favorable low-intensity ultrasound (US) with a power of 210 W was applied to stimulate A. gossypii for 45 min at 33 kHz during the logarithmic phase of 48 h. Compared with those of the untreated A. gossypii, the extracellular riboflavin, total riboflavin, and ...
DOI:
10.1016/j.bej.2022.108394

18. Ensemble models of feedstock blend ratios to minimize supply chain risk in bio-based manufacturing

Author:
Chyi-Shin Chen; Akash Narani; Aigerim Daniyar; Joshua McCauley; Sarah Brown; Todd Pray; Deepti Tanjore
Source:
Biochemical engineering journal 2022 v.181 pp. 107896
ISSN:
1369-703X
Subject:
biomass; biorefining; feedstocks; glucose; lignocellulose; models; regression analysis; risk; supply chain; Florida; Kansas
Abstract:
... Feedstock blending as a strategy to mitigate risks in the supply of lignocellulosic biomass to commercial scale biorefineries across various geographical areas in the United States. Machine learning predictive models estimate sugar yields from feedstock blends expected to be available in Florida and Kansas. Performance of each model was assessed based on feature selection along with two-stack ense ...
DOI:
10.1016/j.bej.2020.107896

19. Harnessing transcription factor Mga2 and fatty acid elongases to overproduce palmitoleic acid in Saccharomyces cerevisiae

Author:
Yue Zhang; Jie Pang; Shuang Liu; Kaili Nie; Li Deng; Fang Wang; Junfeng Liu
Source:
Biochemical engineering journal 2022 v.181 pp. 108402
ISSN:
1369-703X
Subject:
Saccharomyces cerevisiae; anabolism; fatty acid elongase; industry; medicine; oleic acid; palmitoleic acid; transcription factors
Abstract:
... As a long chain monounsaturated fatty acid, palmitoleic acid (POA) can be widely applied in medicine, food and industry. However, the limited natural sources and relatively low content thereof constrain the widespread use of POA. Here we presented the construction of a Saccharomyces cerevisiae platform strain for high-level production of POA. Transcription factor Mga2 increased the contents of POA ...
DOI:
10.1016/j.bej.2022.108402

20. Integrated autolysis, DNA hydrolysis and precipitation enables an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral and clinical-stage anti-COVID-19 candidate

Author:
John S. Decker; Romel Menacho-Melgar; Michael D. Lynch
Source:
Biochemical engineering journal 2022 v.181 pp. 108403
ISSN:
1369-703X
Subject:
COVID-19 infection; DNA; Escherichia coli; autohydrolysis; autolysis; bioprocessing; chromatography; endotoxins; equipment; fermentation; industry; pandemic
Abstract:
... Across the biomanufacturing industry, innovations are needed to improve efficiency and flexibility, especially in the face of challenges such as the COVID-19 pandemic. Here we report an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral currently in clinical trials for COVID-19. Q-Griffithsin is produced at high titer in E. coli and purified to anticipated clinical grade without con ...
DOI:
10.1016/j.bej.2022.108403