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You searched for: Journal name Biochemical engineering journal Remove constraint Journal name: Biochemical engineering journal Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Source 2019 v.144 Remove constraint Source: 2019 v.144
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1. Deciphering electron-shuttling characteristics of microalgal metabolites upon bioelectricity-generating community in microbial fuel cells

Author:
Bin Xu; Qing-Jiang Sun; John Chi-Wei Lan; Min-Hao Chang; Chung-Chuan Hsueh; Bor-Yann Chen
Source:
Biochemical engineering journal 2019 v.144 pp. 148-156
ISSN:
1369-703X
Subject:
Chlorella; bioelectricity; bioenergy; developmental stages; electrochemistry; metabolites; microalgae; microbial communities; microbial fuel cells; species diversity
Abstract:
... This first-attempt study used novel electrochemical alternatives to decipher stimulating effects of microalgal metabolites toward interspecific interactions in microbial fuel cells (MFCs). Extracellular metabolites of Chlorella sp. could stimulate electroactive activity of electron shuttling toward bioelectricity generation in MFCs. Whether such metabolites could express stimulating potency toward ...
DOI:
10.1016/j.bej.2019.01.018

2. Development of bioprocesses for the integral valorisation of fish discards

Author:
José Antonio Vázquez; Andrea Fernández-Compás; María Blanco; Isabel Rodríguez-Amado; Helena Moreno; Javier Borderías; Ricardo I. Pérez-Martín
Source:
Biochemical engineering journal 2019 v.144 pp. 198-208
ISSN:
1369-703X
Subject:
Pediococcus; antihypertensive effect; bioprocessing; biotransformation; culture media; fish; fish discards; fish oils; fish protein hydrolysate; gelatin; lactic acid; lactic acid bacteria; organic nitrogen; peptones
Abstract:
... In the present work, we have developed a set of sequential and complementary alternatives to valorise five fish discards in order to recover and produce fish mince, oils, gelatins, fish protein hydrolysates (FPHs) with antioxidant and antihypertensive properties and marine peptones. In this last case, microbial bioconversion of marine peptones was studied by Pediococcus acidilacti growth, a well-k ...
DOI:
10.1016/j.bej.2019.02.004

3. Marine psychrophile-derived cold-active polygalacturonase: Enhancement of productivity in Thalassospira frigidphilosprofundus S3BA12 by whole cell immobilization

Author:
Vijayanand Adapa; Krishnakanth Pulicherla; KRS Sambasiva Rao
Source:
Biochemical engineering journal 2019 v.144 pp. 135-147
ISSN:
1369-703X
Subject:
Thalassospira; aquatic organisms; dissociation; foams; mechanistic models; polygalacturonase; polyurethanes; salinity
Abstract:
... The productivity of cold-active polygalacturonase (PGase) in a newly identified marine psychrophile, Thalassospira frigidphilosprofundus S3BA12, was increased multifold by employing high-cell-density (HCD) culturing in a simulation-assisted, custom-designed single-stage-continuous immobilized reactor. The reactor was made “halophilic culturing” ready by using surface altered polyurethane foam (PUF ...
DOI:
10.1016/j.bej.2019.01.019

4. Maximizing phosphorus and potassium recycling by supplementation of lignin combustion ash from dry biorefining of lignocellulose

Author:
Gang Liu; Jie Bao
Source:
Biochemical engineering journal 2019 v.144 pp. 104-109
ISSN:
1369-703X
Subject:
agricultural land; biogeochemical cycles; biomass; biorefining; byproducts; combustion; electricity generation; ethanol; ethanol fuels; fertilizer application; lignin; lignocellulose; market prices; nutrients; phosphates; phosphorus; phosphorus fertilizers; potassium; potassium fertilizers; soluble phosphorus; wastewater; China
Abstract:
... Lignocellulose biorefinery produces fuel ethanol and lignin residue byproduct. The combustion ash of lignin residue after electricity generation contains high amount of phosphorous and potassium elements and the recycling to farmland provides a sustainable nutrients supply. This study demonstrated a maximum scenario of water- soluble phosphorous and potassium recycling through a dry biorefining pr ...
DOI:
10.1016/j.bej.2019.01.011

5. Multipurpose fixed-bed bioreactor to simplify lipase production by solid-state fermentation and application in biocatalysis

Author:
Sabrini N.S. Ávila; Melissa L.E. Gutarra; Roberto Fernandez-Lafuente; Elisa D.C. Cavalcanti; Denise M.G. Freire
Source:
Biochemical engineering journal 2019 v.144 pp. 1-7
ISSN:
1369-703X
Subject:
Rhizomucor miehei; aeration; air; babassu oil cake; biocatalysis; bioreactors; carboxylic ester hydrolases; enzyme activity; freeze drying; solid state fermentation; storage quality
Abstract:
... A multipurpose fixed-bed bioreactor was evaluated, to simplify the process of producing lipase and its application in biocatalysis. Rhizomucor miehei lipase was produced by solid-state fermentation (SSF) in the fixed-bed bioreactor and generated a fermented solid with 30 U/g lipase activity and a synthesis capacity of 80% (conversion at 6 h of reaction in a standard stirred reactor). The fermented ...
DOI:
10.1016/j.bej.2018.12.024

6. Oxygen mass transfer and post-denitrification in a modified rotating drum biological contactor

Author:
Ning Li; Wei Zeng; Yanan Yang; Baogui Wang; Zaixing Li; Yongzhen Peng
Source:
Biochemical engineering journal 2019 v.144 pp. 48-56
ISSN:
1369-703X
Subject:
Nitrosomonas europaea; aerobic conditions; ammonium nitrogen; anaerobic ammonium oxidation; bacteria; denitrification; dissolved oxygen; drums (equipment); liquids; mass transfer; oxygen; sewage; submergence; wastewater; wastewater treatment
Abstract:
... A lab-scale rotating drum biological contactor (RDBC) was implemented and accessed concerning abiotic oxygen transfer rate and treatment capacity for domestic sewage. The dissolved oxygen (DO) transfer test showed that the rotational speed and the submergence level of drum greatly affected the DO in bulk liquid with the volumetric oxygen transfer coefficient KLa of 2.3-25.9 h−1, effectively achiev ...
DOI:
10.1016/j.bej.2019.01.008

7. Pilot-scale study using biotrickling filter to remove H2S from sewage lift station: Experiment and CFD simulation

Author:
YiQing Chen; Le Xie; WangFeng Cai; JianWei Wu
Source:
Biochemical engineering journal 2019 v.144 pp. 177-184
ISSN:
1369-703X
Subject:
biodegradation; biofilters; biomass; community structure; deodorization; fluid mechanics; hydrogen sulfide; kinetics; microbial communities; porous media; sewage; simulation models
Abstract:
... In this study, a pilot-scale biotrickling filter (BTF) was developed to remove poisonous gas contaminated by hydrogen sulfide (H2S) from a sewage lift station. The BTF provided a smooth start-up with an increase in the inlet H2S concentration and delivered a stable operation for more than 300 days. The biomass concentration and microbial community structures were analyzed to determine the biodegra ...
DOI:
10.1016/j.bej.2019.02.003

8. Stepwise increase in the production of 13R-manoyl oxide through metabolic engineering of Saccharomyces cerevisiae

Author:
Xiaoyan Guo; Jingjing Liu; Chuanbo Zhang; Fanglong Zhao; Wenyu Lu
Source:
Biochemical engineering journal 2019 v.144 pp. 73-80
ISSN:
1369-703X
Subject:
Saccharomyces cerevisiae; batch fermentation; biosynthesis; carbon; enzymes; feeding methods; forskolin; gene expression regulation; genes; genetically engineered microorganisms; glucose; labdane; medicinal properties; metabolic engineering; oxidation; squalene; synthesis; synthetic biology
Abstract:
... Forskolin, a labdane diterpenoid, possesses a wide range of pharmacological activities. (13R)-manoyl oxide (13R-MO) is the precursor of forskolin. As forskolin is a structurally complex, highly oxidized compound, chemical synthesis is tedious and difficult. Herein, we present a biosynthesis method involving metabolic engineering of Saccharomyces cerevisiae to produce 13R-MO at an initial titer of ...
DOI:
10.1016/j.bej.2019.01.012

9. Systematically redesigning and optimizing the expression of D-lactate dehydrogenase efficiently produces high-optical-purity D-lactic acid in Saccharomyces cerevisiae

Author:
Wei Zhong; Maohua Yang; Tingzhen Mu; Fan Wu; Xuemi Hao; Ruonan Chen; Moustafa Mohamed Sharshar; Anders Thygesen; Qinhong Wang; Jianmin Xing
Source:
Biochemical engineering journal 2019 v.144 pp. 217-226
ISSN:
1369-703X
Subject:
Saccharomyces cerevisiae; acid tolerance; batch fermentation; catalytic activity; genes; glucose; lactate dehydrogenase; lactic acid; mutants; plasmids; pyruvic acid; synthetic biology; thermal stability; transposons; yeasts
Abstract:
... D-lactic acid (D-LA) is gaining increased attention as it can improve the thermostability of poly lactic acid. Acid-tolerant Saccharomyces cerevisiae is a good host for D-LA fermentation. High catalytic efficiency of D-lactate dehydrogenase (D-LDH, EC 1.1.1.28) is crucial for the production of D-LA in yeast. Here, a synthetic biology approach was used to construct high-producing D-LA strains by re ...
DOI:
10.1016/j.bej.2018.09.013

10. Techno-economic evaluation of heat integrated second generation bioethanol and furfural coproduction

Author:
Md Shahadat Hossain; Constantinos Theodoropoulos; Abu Yousuf
Source:
Biochemical engineering journal 2019 v.144 pp. 89-103
ISSN:
1369-703X
Subject:
bioethanol; biomass; computer software; corn stover; economic feasibility; energy efficiency; fossil fuels; furfural; heat; lignocellulose; liquids; profitability; surveys
Abstract:
... In a quest of alternative energy source of fossil fuels, lignocellulosic biomass is intensively studying to produce different liquid fuels and chemicals. Both fuel (bioethanol) and chemical (furfural) were produced simultaneously in this study through two different production routes, biochemical and thermochemical route. Actually, this study firstly defined production pathway in details for each p ...
DOI:
10.1016/j.bej.2019.01.017

11. Virus-like organosilica nanoparticles for lipase immobilization: Characterization and biocatalytic applications

Author:
Yanjun Jiang; Huan Liu; Lihui Wang; Liya Zhou; Zhihong Huang; Li Ma; Ying He; Lujing Shi; Jing Gao
Source:
Biochemical engineering journal 2019 v.144 pp. 125-134
ISSN:
1369-703X
Subject:
Pseudozyma antarctica; alcohols; carboxylic ester hydrolases; esterification; glutaraldehyde; hydrolysis; hydrophobic bonding; hydrophobicity; immobilized enzymes; levulinic acid; nanoparticles; nanotubes; pH; porous media; response surface methodology; silica; solvents; storage quality; temperature; thermal stability
Abstract:
... Hydrophobic virus-like organosilica nanoparticles (VOSNs) with a spherical core formed by mesoporous organosilica surrounded by epitaxial perpendicular nanotubes were successfully synthesized for the first time. These hydrophobic VOSNs were used for the immobilization of the lipase B from Candida antarctica (CALB) via covalent binding method, where glutaraldehyde was used as a spacer to form coval ...
DOI:
10.1016/j.bej.2019.01.022

12. A comparative study of the anaerobic baffled reactor and an integrated anaerobic baffled reactor and microbial electrolysis cell for treatment of petrochemical wastewater

Author:
Amin Arvin; Morteza Hosseini; Mohammad Mehdi Amin; Ghasem Najafpour Darzi; Younes Ghasemi
Source:
Biochemical engineering journal 2019 v.144 pp. 157-165
ISSN:
1369-703X
Subject:
volatile fatty acids; methane production; wastewater; methane; microbial electrolysis cells; pH; chemical oxygen demand; methanogens
Abstract:
... The aim of this study was to evaluate and compare the treatment of petrochemical wastewater with an integrated anaerobic baffled reactor and microbial electrolysis cell (ABR-MEC) and anaerobic baffled reactor (ABR). For this purpose, the effect of hydraulic retention time (HRT) and applied voltage on the performance of ABR-MEC reactor were investigated in four phases. Regarding the results, the ma ...
DOI:
10.1016/j.bej.2019.01.021

13. The electrochemical glucose sensing based on the chitosan-carbon nanotube hybrid

Author:
Young-Bong Choi; Han-Sem Kim; Won-Yong Jeon; Bo-Hee Lee; Ueon Sang Shin; Hyug-Han Kim
Source:
Biochemical engineering journal 2019 v.144 pp. 227-234
ISSN:
1369-703X
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; X-ray photoelectron spectroscopy; ascorbic acid; carbon nanotubes; chemical bonding; chitosan; dielectric spectroscopy; electrochemistry; electrodes; electron transfer; electrons; glucose; glucose oxidase; hydrophilicity; pH; permeability; protocols; redox potential; scanning electron microscopy; uric acid
Abstract:
... In glucose sensors, the direct electron transfer (DET) method delivers electrons from glucose oxidase (GOx) to an electrode without the intervention of a mediator. Many researchers have developed the DET method using carbon nanotube (CNT)-based composites for the permeation into the enzyme co-factor. We synthesized chitosan (Chit) core-shelled CNTs that were hydrophilic, biocompatible, and easily ...
DOI:
10.1016/j.bej.2018.10.021

14. A robust feeding control strategy adjusted and optimized by a neural network for enhancing of alpha 1-antitrypsin production in Pichia pastoris

Author:
Tina Tavasoli; Sareh Arjmand; Seyed Omid Ranaei Siadat; Seyed Abbas Shojaosadati; Abbas Sahebghadam Lotfi
Source:
Biochemical engineering journal 2019 v.144 pp. 18-27
ISSN:
1369-703X
Subject:
protocols; alcohol oxidase; Komagataella pastoris; feeding methods; humans; hosts; methanol; monitoring; recombinant proteins; ammonium hydroxide; process control; specific growth rate
Abstract:
... Exact monitoring of the key process variables, such as specific growth rate (μ), is essential for effective process control, and maintenance of the optimal conditions for the production of recombinant proteins. In this study, a novel and confident on-line μ-stat approach for setting up methanol feeding strategy is described that is based on the consumption of ammonium hydroxide as feedback control ...
DOI:
10.1016/j.bej.2019.01.005

15. Biosensor-assisted high performing cell selection using an E. coli toxin/antitoxin system

Author:
Xiaonan Wang; Avaniek Cabales; Zhenghong Li; Haoran Zhang
Source:
Biochemical engineering journal 2019 v.144 pp. 110-118
ISSN:
1369-703X
Subject:
Escherichia coli; antibiotics; antitoxins; biosensors; genes; metabolic engineering; monitoring; phenylalanine; synthetic biology; tryptophan
Abstract:
... Selection for high-producing cells in a mixed population is of great significance for synthetic biology and metabolic engineering applications. Here, we developed a cell selection mechanism that utilized a product-responsive biosensor to control the expression of E. coli endogenous toxin hipA or antitoxin hipB genes for selective removal of low-performing cells. This approach eliminates the use of ...
DOI:
10.1016/j.bej.2019.01.016

16. CHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant

Author:
Cher H. Goey; David Bell; Cleo Kontoravdi
Source:
Biochemical engineering journal 2019 v.144 pp. 185-192
ISSN:
1369-703X
Subject:
apoptosis; bioreactors; cell culture; culture flasks; hypothermia; immunoglobulin G; protein composition; protein secretion
Abstract:
... Several studies on the impact of cell culture parameters on the profile of host cell protein (HCP) impurities have been carried out in shake flasks. Herein, we explore how transferable the findings and conclusions of such investigations are to lab-scale bioreactors. Experiments were performed in both systems in fed-batch mode under physiological temperature and with a shift to mild hypothermia and ...
DOI:
10.1016/j.bej.2019.02.006

17. Energy efficient technology for enhanced growth and lipid production in Chlamydomonas reinhardtii through additional reflector coated LED photo-bioreactor

Author:
Ajayan K.V.; Anjula K.; Syamasurya A.P.; Harilal C.C.
Source:
Biochemical engineering journal 2019 v.144 pp. 81-88
ISSN:
1369-703X
Subject:
Chlamydomonas reinhardtii; Fourier transform infrared spectroscopy; algae; algae culture; energy efficiency; fluorescent lamps; green light; light intensity; lipid content; lipids; photobioreactors; pigments; specific growth rate
Abstract:
... Silver tinted multi-folded reflector sheet was additionally used in the newly designed LED-PBR setup to study the growth performances and resultant lipid production in Chlamydomonas reinhardtii. The experiment was also carried out to compare the algal growth under various LED light qualities with a white fluorescent lamp (FLs). The synergistic effect of the direct and reflected light provided more ...
DOI:
10.1016/j.bej.2019.01.010

18. Immobilization of Enterobacter aerogenes on carbon fiber and activated carbon to study hydrogen production enhancement

Author:
Fatemeh Boshagh; Khosrow Rostami; Nasrin Moazami
Source:
Biochemical engineering journal 2019 v.144 pp. 64-72
ISSN:
1369-703X
Subject:
Enterobacter aerogenes; Fourier transform infrared spectroscopy; activated carbon; carbon fibers; glucose; hydrogen production; physicochemical properties; scanning electron microscopy; transmission electron microscopy
Abstract:
... Hydrogen production by immobilized Enterobacter aerogenes on the carbon fiber (CF), surface modified carbon fiber, granular and powdered activated carbon in batch mode of operation was investigated. The surface morphology and chemical properties of CFs were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and Fourier transform infrared sp ...
DOI:
10.1016/j.bej.2019.01.014

19. Insight to the early-stage adsorption mechanism of moderately thermophilic consortia and intensified bioleaching of chalcopyrite

Author:
Ronghui Liu; Jing Chen; Wenbo Zhou; Haina Cheng; Hongbo Zhou
Source:
Biochemical engineering journal 2019 v.144 pp. 40-47
ISSN:
1369-703X
Subject:
Acidithiobacillus caldus; Leptospirillum ferriphilum; adsorption; bioaugmentation; biofilm; bioleaching; confocal laser scanning microscopy; copper; interspecific competition; leaching; models; quantitative polymerase chain reaction; sorption isotherms
Abstract:
... Biofilm plays an important role on chalcopyrite bioleaching and early-stage adsorption of cells are essential for biofilm formation. In this study, the interspecies interaction and the early adsorption behavior of moderately thermophilic consortia (Ferroplasma thermophilum, Leptospirillum ferriphilum and Acidithiobacillus caldus) were investigated. qPCR and Confocal laser scanning microscopy (CLSM ...
DOI:
10.1016/j.bej.2019.01.009

20. Isolation and identification of new microalgae strains with antibacterial activity on food-borne pathogens. Engineering approach to optimize synthesis of desired metabolites

Author:
Adilson Ricken Schuelter; Alexander Dimitrov Kroumov; Camila Larissa Hinterholz; Adriana Fiorini; Daniela Estelita Goes Trigueros; Eliane Gruska Vendruscolo; Maya Margaritova Zaharieva; Aparecido Nivaldo Módenes
Source:
Biochemical engineering journal 2019 v.144 pp. 28-39
ISSN:
1369-703X
Subject:
Chlorella; Desmodesmus; Micractinium; Tetradesmus; antibacterial properties; bacterial growth; bioactive compounds; biomass; engineering; food pathogens; genomics; growth retardation; hydrophilicity; lighting; metabolites; microalgae; ribosomal DNA; virulent strains
Abstract:
... This paper presents a strategy for isolation, identification and evaluation of microalgae strains synthesizing biologically active compounds (BAC) with antimicrobial activity against food-borne and other pathogens. Water samples containing microalgae were initially submitted to the enrichment process, followed by standard procedure for obtaining pure culture. Purified DNA from the microalgae isola ...
DOI:
10.1016/j.bej.2019.01.007