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You searched for: Journal Biochemical engineering journal Remove constraint Journal: Biochemical engineering journal Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Source 2019 v.144 Remove constraint Source: 2019 v.144
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1. A comparative study of the anaerobic baffled reactor and an integrated anaerobic baffled reactor and microbial electrolysis cell for treatment of petrochemical wastewater

Author:
Amin Arvin; Morteza Hosseini; Mohammad Mehdi Amin; Ghasem Najafpour Darzi; Younes Ghasemi
Source:
Biochemical engineering journal 2019 v.144 pp. 157-165
ISSN:
1369-703X
Subject:
volatile fatty acids; methane production; wastewater; methane; microbial electrolysis cells; pH; chemical oxygen demand; methanogens
Abstract:
... The aim of this study was to evaluate and compare the treatment of petrochemical wastewater with an integrated anaerobic baffled reactor and microbial electrolysis cell (ABR-MEC) and anaerobic baffled reactor (ABR). For this purpose, the effect of hydraulic retention time (HRT) and applied voltage on the performance of ABR-MEC reactor were investigated in four phases. Regarding the results, the ma ...
DOI:
10.1016/j.bej.2019.01.021

2. A rapid regulation with different response intensities of the pmoA gene guarantees process robustness towards methane surges in continuous and feast-famine bioreactors

Author:
Elisa Rodríguez; Juan Carlos López; Patricia Prieto; Laura Merchán; Pedro A. García-Encina; Raquel Lebrero; Raul Muñoz
Source:
Biochemical engineering journal 2019 v.144 pp. 193-197
ISSN:
1369-703X
Subject:
bioreactors; carbon dioxide production; gene expression; genes; greenhouse gases; mass transfer; messenger RNA; methane; methanotrophs; transcription (genetics)
Abstract:
... A rapid recovery of methanotrophic activity from process fluctuations such as surges in CH4 inlet load is of utmost importance in bioreactors to guarantee a robust abatement of this potent greenhouse gas. However, the response of methanotrophs in CH4 abatement engineered ecosystems facing recurrent operational upsets has not been systematically investigated. The genetic and process performance res ...
DOI:
10.1016/j.bej.2019.01.026

3. A robust feeding control strategy adjusted and optimized by a neural network for enhancing of alpha 1-antitrypsin production in Pichia pastoris

Author:
Tina Tavasoli; Sareh Arjmand; Seyed Omid Ranaei Siadat; Seyed Abbas Shojaosadati; Abbas Sahebghadam Lotfi
Source:
Biochemical engineering journal 2019 v.144 pp. 18-27
ISSN:
1369-703X
Subject:
protocols; alcohol oxidase; Komagataella pastoris; feeding methods; humans; hosts; methanol; monitoring; recombinant proteins; ammonium hydroxide; process control; specific growth rate
Abstract:
... Exact monitoring of the key process variables, such as specific growth rate (μ), is essential for effective process control, and maintenance of the optimal conditions for the production of recombinant proteins. In this study, a novel and confident on-line μ-stat approach for setting up methanol feeding strategy is described that is based on the consumption of ammonium hydroxide as feedback control ...
DOI:
10.1016/j.bej.2019.01.005

4. Biosensor-assisted high performing cell selection using an E. coli toxin/antitoxin system

Author:
Xiaonan Wang; Avaniek Cabales; Zhenghong Li; Haoran Zhang
Source:
Biochemical engineering journal 2019 v.144 pp. 110-118
ISSN:
1369-703X
Subject:
Escherichia coli; antibiotics; antitoxins; biosensors; genes; metabolic engineering; monitoring; phenylalanine; synthetic biology; tryptophan
Abstract:
... Selection for high-producing cells in a mixed population is of great significance for synthetic biology and metabolic engineering applications. Here, we developed a cell selection mechanism that utilized a product-responsive biosensor to control the expression of E. coli endogenous toxin hipA or antitoxin hipB genes for selective removal of low-performing cells. This approach eliminates the use of ...
DOI:
10.1016/j.bej.2019.01.016

5. CHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant

Author:
Cher H. Goey; David Bell; Cleo Kontoravdi
Source:
Biochemical engineering journal 2019 v.144 pp. 185-192
ISSN:
1369-703X
Subject:
apoptosis; bioreactors; cell culture; culture flasks; hypothermia; immunoglobulin G; protein composition; protein secretion
Abstract:
... Several studies on the impact of cell culture parameters on the profile of host cell protein (HCP) impurities have been carried out in shake flasks. Herein, we explore how transferable the findings and conclusions of such investigations are to lab-scale bioreactors. Experiments were performed in both systems in fed-batch mode under physiological temperature and with a shift to mild hypothermia and ...
DOI:
10.1016/j.bej.2019.02.006

6. Deciphering electron-shuttling characteristics of microalgal metabolites upon bioelectricity-generating community in microbial fuel cells

Author:
Bin Xu; Qing-Jiang Sun; John Chi-Wei Lan; Min-Hao Chang; Chung-Chuan Hsueh; Bor-Yann Chen
Source:
Biochemical engineering journal 2019 v.144 pp. 148-156
ISSN:
1369-703X
Subject:
Chlorella; bioelectricity; bioenergy; developmental stages; electrochemistry; metabolites; microalgae; microbial communities; microbial fuel cells; species diversity
Abstract:
... This first-attempt study used novel electrochemical alternatives to decipher stimulating effects of microalgal metabolites toward interspecific interactions in microbial fuel cells (MFCs). Extracellular metabolites of Chlorella sp. could stimulate electroactive activity of electron shuttling toward bioelectricity generation in MFCs. Whether such metabolites could express stimulating potency toward ...
DOI:
10.1016/j.bej.2019.01.018

7. Development of bioprocesses for the integral valorisation of fish discards

Author:
José Antonio Vázquez; Andrea Fernández-Compás; María Blanco; Isabel Rodríguez-Amado; Helena Moreno; Javier Borderías; Ricardo I. Pérez-Martín
Source:
Biochemical engineering journal 2019 v.144 pp. 198-208
ISSN:
1369-703X
Subject:
Pediococcus; antihypertensive effect; bioprocessing; biotransformation; culture media; fish; fish discards; fish oils; fish protein hydrolysate; gelatin; lactic acid; lactic acid bacteria; organic nitrogen; peptones
Abstract:
... In the present work, we have developed a set of sequential and complementary alternatives to valorise five fish discards in order to recover and produce fish mince, oils, gelatins, fish protein hydrolysates (FPHs) with antioxidant and antihypertensive properties and marine peptones. In this last case, microbial bioconversion of marine peptones was studied by Pediococcus acidilacti growth, a well-k ...
DOI:
10.1016/j.bej.2019.02.004

8. Energy efficient technology for enhanced growth and lipid production in Chlamydomonas reinhardtii through additional reflector coated LED photo-bioreactor

Author:
Ajayan K.V.; Anjula K.; Syamasurya A.P.; Harilal C.C.
Source:
Biochemical engineering journal 2019 v.144 pp. 81-88
ISSN:
1369-703X
Subject:
Chlamydomonas reinhardtii; Fourier transform infrared spectroscopy; algae; algae culture; energy efficiency; fluorescent lamps; green light; light intensity; lipid content; lipids; photobioreactors; pigments; specific growth rate
Abstract:
... Silver tinted multi-folded reflector sheet was additionally used in the newly designed LED-PBR setup to study the growth performances and resultant lipid production in Chlamydomonas reinhardtii. The experiment was also carried out to compare the algal growth under various LED light qualities with a white fluorescent lamp (FLs). The synergistic effect of the direct and reflected light provided more ...
DOI:
10.1016/j.bej.2019.01.010

9. Immobilization of Enterobacter aerogenes on carbon fiber and activated carbon to study hydrogen production enhancement

Author:
Fatemeh Boshagh; Khosrow Rostami; Nasrin Moazami
Source:
Biochemical engineering journal 2019 v.144 pp. 64-72
ISSN:
1369-703X
Subject:
Enterobacter aerogenes; Fourier transform infrared spectroscopy; activated carbon; carbon fibers; glucose; hydrogen production; physicochemical properties; scanning electron microscopy; transmission electron microscopy
Abstract:
... Hydrogen production by immobilized Enterobacter aerogenes on the carbon fiber (CF), surface modified carbon fiber, granular and powdered activated carbon in batch mode of operation was investigated. The surface morphology and chemical properties of CFs were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and Fourier transform infrared sp ...
DOI:
10.1016/j.bej.2019.01.014

10. Insight to the early-stage adsorption mechanism of moderately thermophilic consortia and intensified bioleaching of chalcopyrite

Author:
Ronghui Liu; Jing Chen; Wenbo Zhou; Haina Cheng; Hongbo Zhou
Source:
Biochemical engineering journal 2019 v.144 pp. 40-47
ISSN:
1369-703X
Subject:
Acidithiobacillus caldus; Leptospirillum ferriphilum; adsorption; bioaugmentation; biofilm; bioleaching; confocal laser scanning microscopy; copper; interspecific competition; leaching; models; quantitative polymerase chain reaction; sorption isotherms
Abstract:
... Biofilm plays an important role on chalcopyrite bioleaching and early-stage adsorption of cells are essential for biofilm formation. In this study, the interspecies interaction and the early adsorption behavior of moderately thermophilic consortia (Ferroplasma thermophilum, Leptospirillum ferriphilum and Acidithiobacillus caldus) were investigated. qPCR and Confocal laser scanning microscopy (CLSM ...
DOI:
10.1016/j.bej.2019.01.009

11. Isolation and identification of new microalgae strains with antibacterial activity on food-borne pathogens. Engineering approach to optimize synthesis of desired metabolites

Author:
Adilson Ricken Schuelter; Alexander Dimitrov Kroumov; Camila Larissa Hinterholz; Adriana Fiorini; Daniela Estelita Goes Trigueros; Eliane Gruska Vendruscolo; Maya Margaritova Zaharieva; Aparecido Nivaldo Módenes
Source:
Biochemical engineering journal 2019 v.144 pp. 28-39
ISSN:
1369-703X
Subject:
Chlorella; Desmodesmus; Micractinium; Tetradesmus; antibacterial properties; bacterial growth; bioactive compounds; biomass; engineering; food pathogens; genomics; growth retardation; hydrophilicity; lighting; metabolites; microalgae; ribosomal DNA; virulent strains
Abstract:
... This paper presents a strategy for isolation, identification and evaluation of microalgae strains synthesizing biologically active compounds (BAC) with antimicrobial activity against food-borne and other pathogens. Water samples containing microalgae were initially submitted to the enrichment process, followed by standard procedure for obtaining pure culture. Purified DNA from the microalgae isola ...
DOI:
10.1016/j.bej.2019.01.007

12. Marine psychrophile-derived cold-active polygalacturonase: Enhancement of productivity in Thalassospira frigidphilosprofundus S3BA12 by whole cell immobilization

Author:
Vijayanand Adapa; Krishnakanth Pulicherla; KRS Sambasiva Rao
Source:
Biochemical engineering journal 2019 v.144 pp. 135-147
ISSN:
1369-703X
Subject:
Thalassospira; aquatic organisms; dissociation; foams; mechanistic models; polygalacturonase; polyurethanes; salinity
Abstract:
... The productivity of cold-active polygalacturonase (PGase) in a newly identified marine psychrophile, Thalassospira frigidphilosprofundus S3BA12, was increased multifold by employing high-cell-density (HCD) culturing in a simulation-assisted, custom-designed single-stage-continuous immobilized reactor. The reactor was made “halophilic culturing” ready by using surface altered polyurethane foam (PUF ...
DOI:
10.1016/j.bej.2019.01.019

13. Maximizing phosphorus and potassium recycling by supplementation of lignin combustion ash from dry biorefining of lignocellulose

Author:
Gang Liu; Jie Bao
Source:
Biochemical engineering journal 2019 v.144 pp. 104-109
ISSN:
1369-703X
Subject:
agricultural land; biogeochemical cycles; biomass; biorefining; byproducts; combustion; electricity generation; ethanol; ethanol fuels; fertilizer application; lignin; lignocellulose; market prices; nutrients; phosphates; phosphorus; phosphorus fertilizers; potassium; potassium fertilizers; soluble phosphorus; wastewater; China
Abstract:
... Lignocellulose biorefinery produces fuel ethanol and lignin residue byproduct. The combustion ash of lignin residue after electricity generation contains high amount of phosphorous and potassium elements and the recycling to farmland provides a sustainable nutrients supply. This study demonstrated a maximum scenario of water- soluble phosphorous and potassium recycling through a dry biorefining pr ...
DOI:
10.1016/j.bej.2019.01.011

14. Modeling transcriptional factor cross-talk to understand parabolic kinetics, bimodal gene expression and retroactivity in biosensor design

Author:
Hannah Aris; Shayan Borhani; Devorah Cahn; Colleen O'Donnell; Elizabeth Tan; Peng Xu
Source:
Biochemical engineering journal 2019 v.144 pp. 209-216
ISSN:
1369-703X
Subject:
biosensors; carrying capacity; cell growth; engineering; equations; gene expression; gene expression regulation; genes; hysteresis; kinetics; malonyl coenzyme A; protein degradation; simulation models; students; transcription (genetics); transcription factors
Abstract:
... Transcriptional factor-based biosensor has been widely used to reprogram and adjust cellular activity to the changing environment. These biosensors translate an internal cellular signal to a transcriptional reporter output. Previous examples have demonstrated transcriptional factor (TF) cross-talk could lead to complex gene expression dynamics. In this report, we formulated a mechanism-based kinet ...
DOI:
10.1016/j.bej.2019.02.005

15. Multipurpose fixed-bed bioreactor to simplify lipase production by solid-state fermentation and application in biocatalysis

Author:
Sabrini N.S. Ávila; Melissa L.E. Gutarra; Roberto Fernandez-Lafuente; Elisa D.C. Cavalcanti; Denise M.G. Freire
Source:
Biochemical engineering journal 2019 v.144 pp. 1-7
ISSN:
1369-703X
Subject:
Rhizomucor miehei; aeration; air; babassu oil cake; biocatalysis; bioreactors; carboxylic ester hydrolases; enzyme activity; freeze drying; solid state fermentation; storage quality
Abstract:
... A multipurpose fixed-bed bioreactor was evaluated, to simplify the process of producing lipase and its application in biocatalysis. Rhizomucor miehei lipase was produced by solid-state fermentation (SSF) in the fixed-bed bioreactor and generated a fermented solid with 30 U/g lipase activity and a synthesis capacity of 80% (conversion at 6 h of reaction in a standard stirred reactor). The fermented ...
DOI:
10.1016/j.bej.2018.12.024

16. Oxygen mass transfer and post-denitrification in a modified rotating drum biological contactor

Author:
Ning Li; Wei Zeng; Yanan Yang; Baogui Wang; Zaixing Li; Yongzhen Peng
Source:
Biochemical engineering journal 2019 v.144 pp. 48-56
ISSN:
1369-703X
Subject:
Nitrosomonas europaea; aerobic conditions; ammonium nitrogen; anaerobic ammonium oxidation; bacteria; denitrification; dissolved oxygen; drums (equipment); liquids; mass transfer; oxygen; sewage; submergence; wastewater; wastewater treatment
Abstract:
... A lab-scale rotating drum biological contactor (RDBC) was implemented and accessed concerning abiotic oxygen transfer rate and treatment capacity for domestic sewage. The dissolved oxygen (DO) transfer test showed that the rotational speed and the submergence level of drum greatly affected the DO in bulk liquid with the volumetric oxygen transfer coefficient KLa of 2.3-25.9 h−1, effectively achiev ...
DOI:
10.1016/j.bej.2019.01.008

17. Pilot-scale study using biotrickling filter to remove H2S from sewage lift station: Experiment and CFD simulation

Author:
YiQing Chen; Le Xie; WangFeng Cai; JianWei Wu
Source:
Biochemical engineering journal 2019 v.144 pp. 177-184
ISSN:
1369-703X
Subject:
biodegradation; biofilters; biomass; community structure; deodorization; fluid mechanics; hydrogen sulfide; kinetics; microbial communities; porous media; sewage; simulation models
Abstract:
... In this study, a pilot-scale biotrickling filter (BTF) was developed to remove poisonous gas contaminated by hydrogen sulfide (H2S) from a sewage lift station. The BTF provided a smooth start-up with an increase in the inlet H2S concentration and delivered a stable operation for more than 300 days. The biomass concentration and microbial community structures were analyzed to determine the biodegra ...
DOI:
10.1016/j.bej.2019.02.003

18. Prediction of hyoscyamine content in Datura stramonium L. hairy roots using different modeling approaches: Response Surface Methodology (RSM), Artificial Neural Network (ANN) and Kriging

Author:
Ryad Amdoun; El-Hadi Benyoussef; Ahcene Benamghar; Lakhdar Khelifi
Source:
Biochemical engineering journal 2019 v.144 pp. 8-17
ISSN:
1369-703X
Subject:
Datura stramonium; atropine; exposure duration; kriging; neural networks; prediction; response surface methodology; roots; salicylic acid
Abstract:
... The comparison of different modelling approaches Response Surface Methodology (RSM), Artificial Neural Network (ANN) and Kriging, let to develop a novel ANN-RSM combined approach has been applied to accurately predict the variation of hyoscyamine content and especially to statistically explore the relationship between the response and the factors involved in the experiment of this study. The ANN, ...
DOI:
10.1016/j.bej.2019.01.002

19. Preparation and application of a chemically modified laccase and copper phosphate hybrid flower-like biocatalyst

Author:
Pengbo Zhu; Yingsa Wang; Guiliang Li; Kefeng Liu; Yanxue Liu; Jing He; Jiandu Lei
Source:
Biochemical engineering journal 2019 v.144 pp. 235-243
ISSN:
1369-703X
Subject:
anhydrides; biocatalysis; biocatalysts; biotransformation; byproducts; copper; copper nanoparticles; copper sulfate; enzyme activity; laccase; nanoflowers; pH stability; phosphates; resveratrol; storage quality; thermal stability
Abstract:
... In order to face the increasing demand of laccase with high stability and high performance, laccase was chemically modified by acid anhydride, and copper ion (Ⅱ) was coordinated with the hetero atom of the modified laccase to obtain the nanoflower biocatalyst. The effects of different reaction time, laccase concentration and copper sulfate concentration on the formation of nanoflowers were investi ...
DOI:
10.1016/j.bej.2019.01.020

20. Shift in Bacillus sp. JR3 esterase LipJ activity profile after addition of essential residues from family I.5 thermophilic lipases

Author:
Mónica Estupiñán; Carolina Buruaga; F.I. Javier Pastor; Josefina Martínez; Pilar Diaz
Source:
Biochemical engineering journal 2019 v.144 pp. 166-176
ISSN:
1369-703X
Subject:
Bacillus (bacteria); computer simulation; manganese; models; mutants; site-directed mutagenesis; substrate specificity; temperature profiles; triacylglycerol lipase; zinc
Abstract:
... We recently described LipJ, a mesophilic esterase clustering in a specific clade of bacterial lipase family I.5, bearing the same structure but several distinct sequence motifs. Two mutagenesis approaches were used here to modify LipJ by addition of common features of family 1.5 lipases, searching for changes on the activity profile. Iterative saturation mutagenesis of a residue possibly involved ...
DOI:
10.1016/j.bej.2019.01.023