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- Gómez, Itzcóatl A.; Hernández, Vanessa; Palomares, Laura A.; Ramírez, Octavio T.
- Biochemical engineering journal 2018 v.132 pp. 244-254
- DNA; acids; amino acids; cell culture; centrifugation; composite polymers; erythropoietin; flocculants; flocculation; glycosylation; harvest date; microfiltration; models; monoclonal antibodies; polyacrylamide; polyamines; polyethyleneimine; product quality; sedimentation rate
- ... Centrifugation and microfiltration are the most commonly used steps for initial recovery of recombinant glycoproteins. However, such operations have significant drawbacks at large-scales. Accordingly, the use of flocculants was studied here as an alternative to traditional processes for the clarification of CHO cell cultures producing two model glycoproteins: erythropoietin (EPO) and a monoclonal ...