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- Selvaraj, Jonathan Nimal, et al. Show all 6 Authors
- Biochemical engineering journal 2018 v.129 pp. 74-83
- Bacillus subtilis; additive effect; biocatalysts; bioscouring; calcium; fabrics; half life; hardness; mutagenesis; mutants; pH; pectate lyase; textile industry; thermal stability; wettability
- ... Biocatalysts requires enzymes with high activity and stability under process conditions for efficient application. Several protein improvement strategies were used to improve pectate lyase PEL168 from Bacillus subtilis. Initially, a rationally designed mutant V132F obtained showed 1.7-fold increase in activity with wider pH stability. Meanwhile, highly advantageous mutant K47E selected from a rand ...