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- Liu, Lu; Liu, Yanjun; Zhang, Gong; Ge, Yun; Fan, Xinyuan; Lin, Feng; Wang, Jie; Zheng, Huangtao; Xie, Xiao; Zeng, Xiangmei; Chen, Peng R.
- Biochemistry 2018 v.57 no.4 pp. 446-450
- gain-of-function mutation, etc ; Escherichia coli; antibiotic resistance; bacteria; cycloaddition reactions; Show all 5 Subjects
- ... We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to the metabolically labile photocaging groups inside Escherichia coli, our chemical decaging strategy that relies on the inverse electron-demand Diels–Alder (iDA) reaction is compatible with the intracellular environment of bacteria, which can be a general tool for gain-of-fu ...
- Mosesso, Richard; Dougherty, Dennis A.; Lummis, Sarah C. R.
- Biochemistry 2018 v.57 no.27 pp. 4036-4043
- gain-of-function mutation, etc ; Erwinia; Xenopus laevis; calcium; electrophysiology; eukaryotic cells; ion channels; models; mutants; oocytes; proline; receptors; Show all 12 Subjects
- ... Erwinia ligand-gated ion channel (ELIC) is a bacterial homologue of vertebrate pentameric ligand-gated ion channels (pLGICs) and has proven to be a valuable model for understanding the structure and function of this important protein family. There is nevertheless still a question about whether molecular details can be accurately extrapolated from this protein to those found in eukaryotes. Here we ...