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- Yin, Huifang; Pijning, Tjaard; Meng, Xiangfeng; Dijkhuizen, Lubbert; van Leeuwen, Sander S.
- Biochemistry 2017 v.56 no.24 pp. 3109-3118
- crystal structure; site-directed mutagenesis; food industry; beta-galactosidase; amino acids; enzymatic reactions; Streptococcus pneumoniae; active sites; Bacillus circulans; galactooligosaccharides; mutants; prebiotics; structure-activity relationships
- ... The β-galactosidase enzyme from Bacillus circulans ATCC 31382 BgaD is widely used in the food industry to produce prebiotic galactooligosaccharides (GOS). Recently, the crystal structure of a C-terminally truncated version of the enzyme (BgaD-D) has been elucidated. The roles of active site amino acid residues in β-galactosidase enzyme reaction and product specificity have remained unknown. On the ...
- PubMed Central:
- Lella, Muralikrishna; Mahalakshmi, Radhakrishnan
- Biochemistry 2017 v.56 no.24 pp. 2971-2984
- amino acid sequences; amino acids; membrane proteins; prediction
- ... Every amino acid exhibits a different propensity for distinct structural conformations. Hence, decoding how the primary amino acid sequence undergoes the transition to a defined secondary structure and its final three-dimensional fold is presently considered predictable with reasonable certainty. However, protein sequences that defy the first principles of secondary structure prediction (they atta ...