Jump to Main Content
- Dolgova, Natalia
V.; Yu, Corey; Cvitkovic, John P.; Hodak, Miroslav; Nienaber, Kurt H.; Summers, Kelly L.; Cotelesage, Julien J. H.; Bernholc, Jerzy; Kaminski, George A.; Pickering, Ingrid J.; George, Graham N.; Dmitriev, Oleg Y.
- Biochemistry 2017 v.56 no.24 pp. 3129-3141
- cisplatin; copper; drug therapy; free radicals; glutathione; metabolism; molecular weight; neoplasms; polymers; toxicity; transporters
- ... Copper is an essential nutrient required for many biological processes involved in primary metabolism, but free copper is toxic due to its ability to catalyze formation of free radicals. To prevent toxic effects, in the cell copper is bound to proteins and low molecular weight compounds, such as glutathione, at all times. The widely used chemotherapy agent cisplatin is known to bind to copper-tran ...
- Spahiu, Linda; Ålander, Johan; Ottosson-Wadlund, Astrid; Svensson, Richard; Lehmer, Carina; Armstrong, Richard N.; Morgenstern, Ralf
- Biochemistry 2017 v.56 no.24 pp. 3089-3098
- active sites; binding sites; enzyme activation; equations; glutathione; glutathione transferase
- ... Microsomal glutathione transferase 1 (MGST1) has a unique ability to be activated, ≤30-fold, by modification with sulfhydryl reagents. MGST1 exhibits one-third-of-the-sites reactivity toward glutathione and hence heterogeneous binding to different active sites in the homotrimer. Limited turnover stopped-flow kinetic measurements of the activated enzyme allowed us to more accurately determine the K ...
- PubMed Central: