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- Baas, Bert-Jan; Poddar, Harshwardhan; Geertsema, Edzard
M.; Rozeboom, Henriette J.; de Vries, Marcel P.; Permentier, Hjalmar
P.; Thunnissen, Andy-Mark W.H.; Poelarends, Gerrit J.
- Biochemistry 2015 v.54 no.5 pp. 1219-1232
- crystal structure; decarboxylation; dehalogenation; hydroxybenzaldehyde; oxalic acid; oxidation; oxygen; oxygenases; sequence homology; stable isotopes
- ... The vast majority of characterized oxygenases use bound cofactors to activate molecular oxygen to carry out oxidation chemistry. Here, we show that an enzyme of unknown activity, RhCC from Rhodococcus jostii RHA1, functions as an oxygenase, using 4-hydroxyphenylenolpyruvate as a substrate. This unique and complex reaction yields 3-hydroxy-3-(4-hydroxyphenyl)-pyruvate, 4-hydroxybenzaldehyde, and ox ...