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- Yin, Huifang; Pijning, Tjaard; Meng, Xiangfeng; Dijkhuizen, Lubbert; van Leeuwen, Sander S.
- Biochemistry 2017 v.56 no.24 pp. 3109-3118
- crystal structure; site-directed mutagenesis; food industry; beta-galactosidase; amino acids; enzymatic reactions; Streptococcus pneumoniae; active sites; Bacillus circulans; galactooligosaccharides; mutants; prebiotics; structure-activity relationships
- ... The β-galactosidase enzyme from Bacillus circulans ATCC 31382 BgaD is widely used in the food industry to produce prebiotic galactooligosaccharides (GOS). Recently, the crystal structure of a C-terminally truncated version of the enzyme (BgaD-D) has been elucidated. The roles of active site amino acid residues in β-galactosidase enzyme reaction and product specificity have remained unknown. On the ...
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