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You searched for: Journal name Biochimica et biophysica acta Remove constraint Journal name: Biochimica et biophysica acta Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Source 2019 v.1863 no.1 Remove constraint Source: 2019 v.1863 no.1
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1. Atomistic mechanism of the constitutive activation of PDGFRA via its transmembrane domain

Author:
Anton A. Polyansky; Eduard V. Bocharov; Amélie I. Velghe; Andrey S. Kuznetsov; Olga V. Bocharova; Anatoly S. Urban; Alexander S. Arseniev; Bojan Zagrovic; Jean-Baptiste Demoulin; Roman G. Efremov
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 82-95
ISSN:
0304-4165
Subject:
dimerization; models; mutants; mutation; nuclear magnetic resonance spectroscopy; receptor protein-tyrosine kinase
Abstract:
... Single-point mutations in the transmembrane (TM) region of receptor tyrosine kinases (RTKs) can lead to abnormal ligand-independent activation. We use a combination of computational modeling, NMR spectroscopy and cell experiments to analyze in detail the mechanism of how TM domains contribute to the activation of wild-type (WT) PDGFRA and its oncogenic V536E mutant. Using a computational framework ...
DOI:
10.1016/j.bbagen.2018.09.011

2. Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects

Author:
Sawssen Hajji; Sameh Ben Khedir; Ibtissem Hamza-Mnif; Marwa Hamdi; Ines Jedidi; Rim Kallel; Sami Boufi; Moncef Nasri
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 241-254
ISSN:
0304-4165
Subject:
Chinese hamsters; animal ovaries; antibacterial properties; antioxidant activity; antioxidants; chitosan; cytotoxicity; histology; nanoparticles; nanosilver; oxidative stress; polymers; stabilizers; tissue repair; tissues; transmission electron microscopy
Abstract:
... The aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190–200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the results revealed that they exhibi ...
DOI:
10.1016/j.bbagen.2018.10.010

3. Biophysical simulations and structure-based modeling of residue interaction networks in the tumor suppressor proteins reveal functional role of cancer mutation hotspots in molecular communication

Author:
Gennady M. Verkhivker
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 210-225
ISSN:
0304-4165
Subject:
loss-of-function mutation; models; molecular dynamics; neoplasms; phenotype; protein structure; regression analysis; tumor suppressor genes; tumor suppressor proteins
Abstract:
... In the current study, we have combined molecular simulations and energetic analysis with dynamics-based network modeling and perturbation response scanning to determine molecular signatures of mutational hotspot residues in the p53, PTEN, and SMAD4 tumor suppressor proteins. By examining structure, energetics and dynamics of these proteins, we have shown that inactivating mutations preferentially ...
DOI:
10.1016/j.bbagen.2018.10.009

4. Catechin-mediated restructuring of a bacterial toxin inhibits activity

Author:
En Hyung Chang; Joanne Huang; Zixiang Lin; Angela C. Brown
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 191-198
ISSN:
0304-4165
Subject:
Actinobacillus actinomycetemcomitans; antibacterial properties; bacteria; bacterial toxins; catechin; cholesterol; circular dichroism spectroscopy; confocal microscopy; cytotoxicity; fluorescence; humans; leaves; leukocytes; leukotoxins; mechanism of action; polyphenols; staining; tea; virulence
Abstract:
... Catechins, polyphenols derived from tea leaves, have been shown to have antibacterial properties, through direct killing of bacteria as well as through inhibition of bacterial toxin activity. In particular, certain catechins have been shown to have bactericidal effects on the oral bacterium, Aggregatibacter actinomycetemcomitans, as well as the ability to inhibit a key virulence factor of this org ...
DOI:
10.1016/j.bbagen.2018.10.011
PubMed:
30342156
PubMed Central:
PMC6235716

5. Characterization of a nucleus located mollusc mitoferrin and its response to OsHV-1 infection

Author:
Lusheng Xin; Bowen Huang; Chen Li; Changming Bai; Chongming Wang
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 255-265
ISSN:
0304-4165
Subject:
clams; ferritin; functional properties; genes; immunohistochemistry; iron; iron absorption; mitochondria; pathogens
Abstract:
... Mitoferrin genes as members of SLC25 family are conservatively existed across species, mainly locate on mitochondria and serve an important role in the regulation of whole cellular iron metabolism. Available iron withholding from pathogens presents an important host defense strategy, while the regulation role of mitoferrin against invading pathogens is largely unknown. In this study, a unique moll ...
DOI:
10.1016/j.bbagen.2018.10.014

6. Characterization of an RNase with two catalytic centers. Human RNase6 catalytic and phosphate-binding site arrangement favors the endonuclease cleavage of polymeric substrates

Author:
Guillem Prats-Ejarque; Jose A. Blanco; Vivian A. Salazar; Victòria M. Nogués; Mohammed Moussaoui; Ester Boix
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 105-117
ISSN:
0304-4165
Subject:
Primates; X-ray diffraction; active sites; anions; antimicrobial proteins; binding sites; crystal structure; enzyme activity; histidine; humans; lysine; phosphates; polymers; ribonucleases; site-directed mutagenesis
Abstract:
... Human RNase6 is a small cationic antimicrobial protein that belongs to the vertebrate RNaseA superfamily. All members share a common catalytic mechanism, which involves a conserved catalytic triad, constituted by two histidines and a lysine (His15/His122/Lys38 in RNase6 corresponding to His12/His119/Lys41 in RNaseA). Recently, our first crystal structure of human RNase6 identified an additional Hi ...
DOI:
10.1016/j.bbagen.2018.09.021

7. Deinococcus radiodurans' SRA-HNH domain containing protein Shp (Dr1533) is involved in faithful genome inheritance maintenance following DNA damage

Author:
Alex Ferrandi; Federica Castani; Mauro Pitaro; Sara Tagliaferri; Claire Bouthier de la Tour; Rosa Alduina; Suzanne Sommer; Mauro Fasano; Paola Barbieri; Monica Mancini; Ian Marc Bonapace
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 118-129
ISSN:
0304-4165
Subject:
DNA; DNA damage; DNA repair; Deinococcus radiodurans; Escherichia coli; bioinformatics; cytosine; deoxyribonucleases; enzyme activity; genome; homologous recombination; inheritance (genetics); irradiation; kanamycin; knockout mutants; loci; manganese; molecular cloning; mutagens; oligonucleotides
Abstract:
... Deinococcus radiodurans R1 (DR) survives conditions of extreme desiccation, irradiation and exposure to genotoxic chemicals, due to efficient DNA breaks repair, also through Mn2+ protection of DNA repair enzymes.Possible annotated domains of the DR1533 locus protein (Shp) were searched by bioinformatic analysis. The gene was cloned and expressed as fusion protein. Band-shift assays of Shp or the S ...
DOI:
10.1016/j.bbagen.2018.09.020

8. Identification of amino acid residues critical for the B cell growth-promoting activity of HIV-1 matrix protein p17 variants

Author:
Wangxiao He; Pietro Mazzuca; Weirong Yuan; Kristen Varney; Antonella Bugatti; Alfredo Cagnotto; Cinzia Giagulli; Marco Rusnati; Stefania Marsico; Luisa Diomede; Mario Salmona; Arnaldo Caruso; Wuyuan Lu; Francesca Caccuri
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 13-24
ISSN:
0304-4165
Subject:
B-lymphocytes; HIV infections; Human immunodeficiency virus 1; Western blotting; agar; amino acids; cell proliferation; circular dichroism spectroscopy; denaturation; disulfide bonds; epitopes; non-Hodgkin lymphoma; nuclear magnetic resonance spectroscopy; patients; protein conformation; receptors; signal transduction; surface plasmon resonance; synthetic peptides
Abstract:
... HIV-1 matrix protein p17 variants (vp17s) detected in HIV-1-infected patients with non-Hodgkin's lymphoma (HIV-NHL) display, differently from the wild-type protein (refp17), B cell growth-promoting activity. Biophysical analysis revealed that vp17s are destabilized as compared to refp17, motivating us to explore structure-function relationships.We used: biophysical techniques (circular dichroism ( ...
DOI:
10.1016/j.bbagen.2018.09.016

9. Insulin-like growth factor type I selectively binds to G-quadruplex structures

Author:
Hongbo Chen; Hongxia Sun; Yahong Chai; Suge Zhang; Aijiao Guan; Qian Li; Li Yao; Yalin Tang
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 31-38
ISSN:
0304-4165
Subject:
binding capacity; circular dichroism spectroscopy; energy transfer; fluorescence; fluorescence emission spectroscopy; growth and development; insulin-like growth factor I; ligands; melting; nucleotide motifs; proteins; surface plasmon resonance; therapeutics; topology
Abstract:
... G-quadruplex has been viewed as a promising therapeutic target in oncology due to its potentially important roles in physiological and pathological processes. Emerging evidence suggests that the biological functions of G-quadruplexes are closely related to the binding of some proteins. Insulin-like growth factor type I (IGF-1), as a significant modulator of cell growth and development, may serve a ...
DOI:
10.1016/j.bbagen.2018.09.022

10. Long-chain polyphosphate in osteoblast matrix vesicles: Enrichment and inhibition of mineralization

Author:
Lina Li; Mei Li Khong; Eric L.H. Lui; Saida Mebarek; David Magne; Rene Buchet; Julian A. Tanner
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 199-209
ISSN:
0304-4165
Subject:
4',6-diamidino-2-phenylindole; cartilage; chick embryos; confocal microscopy; enzymatic treatment; eukaryotic cells; fluorescence; gel chromatography; hydroxyapatite; mammals; mineralization; models; osteoblasts; polyacrylamide gel electrophoresis; prokaryotic cells; staining; transmission electron microscopy; urea
Abstract:
... Inorganic polyphosphate (polyP) is a fundamental and ubiquitous molecule in prokaryotes and eukaryotes. PolyP has been found in mammalian tissues with particularly high levels of long-chain polyP in bone and cartilage where critical questions remain as to its localization and function. Here, we investigated polyP presence and function in osteoblast-like SaOS-2 cells and cell-derived matrix vesicle ...
DOI:
10.1016/j.bbagen.2018.10.003

11. Myrtenal-induced V-ATPase inhibition - A toxicity mechanism behind tumor cell death and suppressed migration and invasion in melanoma

Author:
Brunna Xavier Martins; Raul Ferraz Arruda; Gildeíde Aparecida Costa; Hassan Jerdy; Sávio Bastos de Souza; Julianna Maria Santos; William Rodrigues de Freitas; Milton Masahiko Kanashiro; Eulógio Carlos Queiroz de Carvalho; Nadir Francisca Sant'Anna; Fernanda Antunes; Raul Martinez-Zaguilan; Sennoune Souad; Anna Lvovna Okorokova-Façanha; Arnoldo Rocha Façanha
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 1-12
ISSN:
0304-4165
Subject:
H-transporting ATP synthase; acidification; cell death; cell lines; cell membranes; drug therapy; electrochemistry; enzyme inhibition; humans; mechanism of action; melanoma; metastasis; mice; models; monoterpenoids; neoplasm cells; pH; proton pump; proton pump inhibitors; protons; toxicity
Abstract:
... Metastatic tumor cells have acidic extracellular pH and differential electrochemical H+ gradients generated across their cell membranes by V-type H+-ATPases. This study shows that inhibition of the V-ATPases by the plant-derived monoterpene Myrtenal results in tumor cell death and decreased metastatic dissemination in mice.The Myrtenal anticancer toxicity was evaluated in vitro using murine (B16F0 ...
DOI:
10.1016/j.bbagen.2018.09.006

12. Targeting glutathione S-transferase P and its interactome with selenium compounds in cancer therapy

Author:
D. Bartolini; P. Torquato; M. Piroddi; F. Galli
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 130-143
ISSN:
0304-4165
Subject:
Lewis acids; cell death; chemoprevention; drug resistance; drug therapy; enzyme activity; genes; glutathione; glutathione transferase; inflammation; metabolism; neoplasms; peroxidase; selenium; signal transduction; stress response; transcription factors
Abstract:
... The glutathione (GSH) S-transferase family of detoxification and signalling proteins represents a major hub for the metabolism of Selenium-derived compounds. At the same time, these compounds can be used to modulate the expression and multiple activities of GSTs and other glutathione-dependent genes, that are important aspects in both the chemoprevention and therapy of drug-resistant cancers.In th ...
DOI:
10.1016/j.bbagen.2018.09.023

13. ABC transporters as cancer drivers: Potential functions in cancer development

Author:
Alice Domenichini; Aleksandra Adamska; Marco Falasca
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 52-60
ISSN:
0304-4165
Subject:
ABC transporters; bioactive compounds; carcinogenesis; drugs; multiple drug resistance; neoplasms; therapeutics
Abstract:
... ABC transporters have attracted considerable attention for their function as drug transporters in a broad range of tumours and are therefore considered as major players in cancer chemoresistance. However, less attention has been focused on their potential role as active players in cancer development and progression.This review presents the evidence suggesting that ABC transporters might have a mor ...
DOI:
10.1016/j.bbagen.2018.09.019

14. Boron inhibits apoptosis in hyperapoptosis condition: Acts by stabilizing the mitochondrial membrane and inhibiting matrix remodeling

Author:
Indusmita Routray; Shakir Ali
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 144-152
ISSN:
0304-4165
Subject:
DNA damage; abnormal development; apoptosis; boron; caspase-3; cytochrome c; diabetes; membrane potential; mitochondria; mitochondrial membrane; models; neurodegenerative diseases; phosphatidylserines; protective effect
Abstract:
... An abnormally high apoptosis has been associated with a number of clinical conditions including embryonal malformations and various pathologies such as neuronal degeneration and diabetes. In this study, boron is reported to inhibit apoptosis in hyperapoptosis conditions as demonstrated in a model of hyperapoptosis. Boron is a metalloid which is present in food in small amounts and is suggested her ...
DOI:
10.1016/j.bbagen.2018.10.007

15. Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish

Author:
Ignacio Fernández; Jorge M.O. Fernandes; Vânia P. Roberto; Martina Kopp; Catarina Oliveira; Marta F. Riesco; Jorge Dias; Cymon J. Cox; M. Leonor Cancela; Elsa Cabrita; Paulo Gavaia
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 39-51
ISSN:
0304-4165
Subject:
DNA fragmentation; Solea senegalensis; bioinformatics; biomarkers; blood coagulation; blood plasma; brain; dietary supplements; estriol; fish; fish nutrition; gene expression regulation; gonads; males; messenger RNA; non-coding RNA; nutritional status; reproductive performance; skeletal development; sperm quality; spermatogenesis; testosterone; viability; vitamin K
Abstract:
... Vitamin K (VK) is a fat-soluble vitamin known for its essential role in blood coagulation, but also on other biological processes (e.g. reproduction, brain and bone development) have been recently suggested. Nevertheless, the molecular mechanisms behind its particular function on reproduction are not yet fully understood.The potential role of VK on reproduction through nutritional supplementation ...
DOI:
10.1016/j.bbagen.2018.09.017

16. Dimerization of an aptamer generated from Ligand-guided selection (LIGS) yields a high affinity scaffold against B-cells

Author:
Sana Batool; Kimon V. Argyropoulos; Roksana Azad; Precious Okeoma; Hasan Zumrut; Sanam Bhandari; Rigzin Dekhang; Prabodhika R. Mallikaratchy
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 232-240
ISSN:
0304-4165
Subject:
B-lymphocytes; DNA; RNA; antibodies; confocal microscopy; dimerization; image analysis; immunoglobulin M; ligands; neoplasm cells; neoplasms; oligonucleotides; therapeutics
Abstract:
... Nucleic Acid Aptamers (NAAs) are a class of synthetic DNA or RNA molecules that bind specifically to their target. We recently introduced an aptamer termed R1.2 against membrane Immunoglobulin M (mIgM) expressing B-cell neoplasms using Ligand Guided Selection (LIGS). While LIGS-generated aptamers are highly specific, their lower affinity prevents aptamers from being used for translational applicat ...
DOI:
10.1016/j.bbagen.2018.10.006
PubMed:
30342154
PubMed Central:
PMC6248343

17. Insulin adsorption onto zinc oxide nanoparticle mediates conformational rearrangement into amyloid-prone structure with enhanced cytotoxic propensity

Author:
Shreyasi Asthana; Zaved Hazarika; Parth Sarathi Nayak; Jyoti Roy; Anupam Nath Jha; Bibekanand Mallick; Suman Jha
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 153-166
ISSN:
0304-4165
Subject:
X-ray diffraction; adsorption; amyloidosis; calorimetry; cell lines; circular dichroism spectroscopy; cytotoxicity; dye binding; dyes; fluorescence; insulin; molecular dynamics; nanoparticles; pH; patients; proteins; simulation models; titration; transmission electron microscopy; zinc oxide
Abstract:
... Injection localized amyloidosis is one of the most prevalent disorders in type II diabetes mellitus (TIIDM) patients relying on insulin injections. Previous studies have reported that nanoparticles can play a role in the amyloidogenic process of proteins. Hence, the present study deals with the effect of zinc oxide nanoparticles (ZnONP) on the amyloidogenicity and cytotoxicity of insulin.ZnONP is ...
DOI:
10.1016/j.bbagen.2018.10.004

18. Lifetime-based photoconversion of EGFP as a tool for FLIM

Author:
Petr Herman; Aleš Holoubek; Barbora Brodska
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 266-277
ISSN:
0304-4165
Subject:
T-lymphocytes; absorption; biomedical research; cell lines; fluorescence; fluorescent proteins; image analysis; irradiation; mutants; photochemistry; solvents; viscosity
Abstract:
... EGFP is a fluorescent tag extensively used in biological and biomedical research. Over the years many researches have gathered collections of cell lines bearing specific EGFP-tagged proteins. Despite its popularity some photochemical properties of EGFP remain undocumented and unused. We report on so far unexplored lifetime photoconversion of EGFP usable in FLIM.Fluorescence lifetime imaging and sp ...
DOI:
10.1016/j.bbagen.2018.10.016

19. Loss of cytosolic Mg2+ binding sites in the Thermotoga maritima CorA Mg2+ channel is not sufficient for channel opening

Author:
Thomas Kowatz; Michael E. Maguire
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 25-30
ISSN:
0304-4165
Subject:
Thermotoga maritima; binding sites; cobalt; crystal structure; magnesium; mutants; proteins
Abstract:
... The CorA Mg2+ channel is a homopentamer with five-fold symmetry. Each monomer consists of a large cytoplasmic domain and two transmembrane helices connected via a short periplasmic loop. In the Thermotoga maritima CorA crystal structure, a Mg2+ is bound between D89 of one monomer and D253 of the adjacent monomer (M1 binding site). Release of Mg2+ from these sites has been hypothesized to cause ope ...
DOI:
10.1016/j.bbagen.2018.09.001
PubMed:
30293964
PubMed Central:
PMC6342003

20. MV-mimicking micelles loaded with PEG-serine-ACP nanoparticles to achieve biomimetic intra/extra fibrillar mineralization of collagen in vitro

Author:
Minjuan Shen; Mingli Lin; Mengqi Zhu; Wenxin Zhang; Danyang Lu; Huanhuan Liu; Jingjing Deng; Kehua Que; Xu Zhang
Source:
Biochimica et biophysica acta 2019 v.1863 no.1 pp. 167-181
ISSN:
0304-4165
Subject:
biomimetics; biomineralization; bones; calcification; calcium phosphates; cartilage; collagen; micelles; minerals; models; nanoparticles; pH; polysorbates; serine; surface tension; tissue engineering
Abstract:
... Since their discovery, matrix vesicles (MVs) containing minerals have received considerable attention for their role in the mineralization of bone, dentin and calcified cartilage. Additionally, MVs' association with collagen fibrils, which serve as the scaffold for calcification in the organic matrix, has been repeatedly highlighted. The primary purpose of the present study was to establish a MVs– ...
DOI:
10.1016/j.bbagen.2018.10.005