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Biochimica et biophysica acta
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7981-2019
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circular dichroism spectroscopy
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- circular dichroism spectroscopy[remove]19
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- Author:
- Michael H. Chiu; Chunhua Shi; Matthew Rosin; Zarah Batulan; Edward R. O'Brien
- Source:
- Biochimica et biophysica acta 2019 v.1863 no.10 pp. 1536-1546
- ISSN:
- 0304-4165
- Subject:
- animal models; atherogenesis; atherosclerosis; autoantibodies; cholesterol; circular dichroism spectroscopy; dephosphorylation; heat shock proteins; humans; immunoglobulin G; inflammation; light scattering; mutants; particle size; peptides; phosphorylation; prediction
- Abstract:
- ... Heat Shock Protein 27 (HSP27) is a small molecular chaperone that reduces the development of atherosclerosis by lowering plasma cholesterol levels as well as inflammation. Human studies show an inverse correlation between atherosclerotic burden and HSP27 expression, and are supported by murine models in which augmenting HSP27 levels curbs experimental atherogenesis. Natural HSP27 auto-antibodies ( ...
- DOI:
- 10.1016/j.bbagen.2019.05.016
- https://doi.org/10.1016/j.bbagen.2019.05.016
- Author:
- En Hyung Chang; Joanne Huang; Zixiang Lin; Angela C. Brown
- Source:
- Biochimica et biophysica acta 2019 v.1863 no.1 pp. 191-198
- ISSN:
- 0304-4165
- Subject:
- Actinobacillus actinomycetemcomitans; antibacterial properties; bacteria; bacterial toxins; catechin; cholesterol; circular dichroism spectroscopy; confocal microscopy; cytotoxicity; fluorescence; humans; leaves; leukocytes; leukotoxins; mechanism of action; polyphenols; staining; tea; virulence
- Abstract:
- ... Catechins, polyphenols derived from tea leaves, have been shown to have antibacterial properties, through direct killing of bacteria as well as through inhibition of bacterial toxin activity. In particular, certain catechins have been shown to have bactericidal effects on the oral bacterium, Aggregatibacter actinomycetemcomitans, as well as the ability to inhibit a key virulence factor of this org ...
- DOI:
- 10.1016/j.bbagen.2018.10.011
- PubMed:
- 30342156
- PubMed Central:
- PMC6235716
- https://doi.org/10.1016/j.bbagen.2018.10.011
3. Cation-dependent changes in the thylakoid membrane appression of the diatom Thalassiosira pseudonana
- Author:
- Stefanie Jäger; Claudia Büchel
- Source:
- Biochimica et biophysica acta 2019 v.1860 no.1 pp. 41-51
- ISSN:
- 0005-2728
- Subject:
- Bacillariophyceae; Thalassiosira pseudonana; cations; circular dichroism spectroscopy; electrostatic interactions; fluorescence emission spectroscopy; grana; light harvesting complex; magnesium; photosystem II; salts; thylakoids
- Abstract:
- ... Diatoms show a special organisation of their plastid membranes, such that their thylakoids span the entire plastid in bands of three. While in higher plants the interaction of the light harvesting complex II and photosystem II with divalent cations (especially Mg2+) was found to take part in the interplay of electrostatic attraction and repulsion in grana membrane appression, for diatoms the key p ...
- DOI:
- 10.1016/j.bbabio.2018.11.003
- https://doi.org/10.1016/j.bbabio.2018.11.003
- Author:
- I-Te Chu; Chia-Chuan Wu; Ta-Chau Chang
- Source:
- Biochimica et biophysica acta 2019 v.1863 no.2 pp. 418-425
- ISSN:
- 0304-4165
- Subject:
- circular dichroism spectroscopy; mitochondria; mitochondrial DNA; mutation; neoplasms; neurodegenerative diseases; nuclear magnetic resonance spectroscopy; nucleotide sequences; polyacrylamide gel electrophoresis; potassium
- Abstract:
- ... Mitochondrial DNA (mtDNA) mutations could lead to mitochondrial dysfunction, which plays a major role in aging, neurodegeneration, and cancer. Recently, we have highlighted G-quadruplex (G4) formation of putative G4-forming (PQF) mtDNA sequences in cells. Herein, we examine structural variation of G4 formation due to mutation of mtDNA sequences in vitro.The combined circular dichroism (CD), nuclea ...
- DOI:
- 10.1016/j.bbagen.2018.11.009
- https://doi.org/10.1016/j.bbagen.2018.11.009
- Author:
- Wangxiao He; Pietro Mazzuca; Weirong Yuan; Kristen Varney; Antonella Bugatti; Alfredo Cagnotto; Cinzia Giagulli; Marco Rusnati; Stefania Marsico; Luisa Diomede; Mario Salmona; Arnaldo Caruso; Wuyuan Lu; Francesca Caccuri
- Source:
- Biochimica et biophysica acta 2019 v.1863 no.1 pp. 13-24
- ISSN:
- 0304-4165
- Subject:
- B-lymphocytes; HIV infections; Human immunodeficiency virus 1; Western blotting; agar; amino acids; cell proliferation; circular dichroism spectroscopy; denaturation; disulfide bonds; epitopes; non-Hodgkin lymphoma; nuclear magnetic resonance spectroscopy; patients; protein conformation; receptors; signal transduction; surface plasmon resonance; synthetic peptides
- Abstract:
- ... HIV-1 matrix protein p17 variants (vp17s) detected in HIV-1-infected patients with non-Hodgkin's lymphoma (HIV-NHL) display, differently from the wild-type protein (refp17), B cell growth-promoting activity. Biophysical analysis revealed that vp17s are destabilized as compared to refp17, motivating us to explore structure-function relationships.We used: biophysical techniques (circular dichroism ( ...
- DOI:
- 10.1016/j.bbagen.2018.09.016
- https://doi.org/10.1016/j.bbagen.2018.09.016
- Author:
- Benazir Alam; Sampa Biswas
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.9 pp. 854-865
- ISSN:
- 1570-9639
- Subject:
- Plasmodium falciparum; cathepsin L; cell membranes; circular dichroism spectroscopy; computer simulation; cysteine; electrostatic interactions; energy; enzyme activity; erythrocytes; gel chromatography; hemoglobin; humans; mutants; papain; parasites; proteolysis; serpins; stoichiometry
- Abstract:
- ... Falcipain-2(FP2), a cysteine protease from Plasmodium falciparum, cleaves host erythrocyte hemoglobin and specific membrane skeleton components during the parasite life cycle. Therefore its inhibition has been considered as an attractive approach to combat the disease. SerpinB3 (SPB3) belongs to the ovalbumin-serpin family and is a potent cross-class inhibitor of cysteine cathepsins L, K, S and pa ...
- DOI:
- 10.1016/j.bbapap.2019.06.012
- https://doi.org/10.1016/j.bbapap.2019.06.012
- Author:
- Katarina Siposova; Tibor Kozar; Veronika Huntosova; Silvia Tomkova; Andrey Musatov
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.3 pp. 259-274
- ISSN:
- 1570-9639
- Subject:
- amyloid; atomic force microscopy; binding sites; cell viability; circular dichroism spectroscopy; curcumin; epigallocatechin gallate; fluorescence emission spectroscopy; inhibitory concentration 50; insulin; lysozyme; models; polyphenols; protocols
- Abstract:
- ... Natural polyphenols, curcumin, rottlerin and EGCG were selected for initial computational modeling of protein-ligand interaction patterns. The docking calculations demonstrated that these polyphenols can easily adjust their conformational shape to fit well into the binding sites of amyloidogenic proteins. The experimental part of the study focused on the effect of rottlerin on fibrillation of thre ...
- DOI:
- 10.1016/j.bbapap.2018.10.002
- https://doi.org/10.1016/j.bbapap.2018.10.002
- Author:
- Shreyasi Asthana; Zaved Hazarika; Parth Sarathi Nayak; Jyoti Roy; Anupam Nath Jha; Bibekanand Mallick; Suman Jha
- Source:
- Biochimica et biophysica acta 2019 v.1863 no.1 pp. 153-166
- ISSN:
- 0304-4165
- Subject:
- X-ray diffraction; adsorption; amyloidosis; calorimetry; cell lines; circular dichroism spectroscopy; cytotoxicity; dye binding; dyes; fluorescence; insulin; molecular dynamics; nanoparticles; pH; patients; proteins; simulation models; titration; transmission electron microscopy; zinc oxide
- Abstract:
- ... Injection localized amyloidosis is one of the most prevalent disorders in type II diabetes mellitus (TIIDM) patients relying on insulin injections. Previous studies have reported that nanoparticles can play a role in the amyloidogenic process of proteins. Hence, the present study deals with the effect of zinc oxide nanoparticles (ZnONP) on the amyloidogenicity and cytotoxicity of insulin.ZnONP is ...
- DOI:
- 10.1016/j.bbagen.2018.10.004
- https://doi.org/10.1016/j.bbagen.2018.10.004
- Author:
- Hongbo Chen; Hongxia Sun; Yahong Chai; Suge Zhang; Aijiao Guan; Qian Li; Li Yao; Yalin Tang
- Source:
- Biochimica et biophysica acta 2019 v.1863 no.1 pp. 31-38
- ISSN:
- 0304-4165
- Subject:
- binding capacity; circular dichroism spectroscopy; energy transfer; fluorescence; fluorescence emission spectroscopy; growth and development; insulin-like growth factor I; ligands; melting; nucleotide motifs; proteins; surface plasmon resonance; therapeutics; topology
- Abstract:
- ... G-quadruplex has been viewed as a promising therapeutic target in oncology due to its potentially important roles in physiological and pathological processes. Emerging evidence suggests that the biological functions of G-quadruplexes are closely related to the binding of some proteins. Insulin-like growth factor type I (IGF-1), as a significant modulator of cell growth and development, may serve a ...
- DOI:
- 10.1016/j.bbagen.2018.09.022
- https://doi.org/10.1016/j.bbagen.2018.09.022
- Author:
- Kostas Stamatakis; Panayiotis-Ilias Broussos; Angeliki Panagiotopoulou; Rebecca J. Gast; Maria Pelecanou; George C. Papageorgiou
- Source:
- Biochimica et biophysica acta 2019 v.1860 no.1 pp. 102-110
- ISSN:
- 0005-2728
- Subject:
- Bacillariophyceae; Chlorophyta; Cyanobacteria; Miozoa; Phaeocystis; Rhodophyta; absorption; algae; amino acids; autotrophs; chlorophyll; circular dichroism spectroscopy; fluorescence; kleptoplasts; photosynthesis; ultraviolet radiation; Antarctica
- Abstract:
- ... Light state transitions (STs) is a reversible physiological process that oxygenic photosynthetic organisms use in order to minimize imbalances in the electronic excitation delivery to the reaction centers of Photosystems I and II, and thus to optimize photosynthesis. STs have been studied extensively in plants, green algae, red algae and cyanobacteria, but sparsely in algae with secondary red alga ...
- DOI:
- 10.1016/j.bbabio.2018.11.016
- https://doi.org/10.1016/j.bbabio.2018.11.016
- Author:
- Jannik Nedergaard Pedersen; Zhiping Jiang; Gunna Christiansen; Jennifer C. Lee; Jan Skov Pedersen; Daniel E. Otzen
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.5 pp. 519-528
- ISSN:
- 1570-9639
- Subject:
- amyloid; biosynthesis; calorimetry; circular dichroism spectroscopy; electron microscopy; fluorescence; liquid petroleum gas; lysophosphatidylcholine; melanin; micelles; organelles; protein structure; small-angle X-ray scattering; titration; zwitterions
- Abstract:
- ... Lipids often play an important role in the initial steps of fibrillation. The melanosomal protein Pmel17 forms amyloid in vivo and contains a highly amyloidogenic Repeat domain (RPT), important for melanin biosynthesis. RPT fibrillation is influenced by two lysolipids, the anionic lysophosphatidylglycerol (LPG) and zwitterionic lysophosphatidylcholine (LPC), both present in vivo at elevated concen ...
- DOI:
- 10.1016/j.bbapap.2018.11.007
- PubMed:
- 30471451
- PubMed Central:
- PMC6677129
- https://doi.org/10.1016/j.bbapap.2018.11.007
- Author:
- Kseniya Barinova; Marina Serebryakova; Evgeny Sheval; Elena Schmalhausen; Vladimir Muronetz
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.4 pp. 396-404
- ISSN:
- 1570-9639
- Subject:
- Parkinson disease; aldehydes; amyloid; circular dichroism spectroscopy; diabetes; fluorescence; fluorescence emission spectroscopy; glyceraldehyde 3-phosphate; glyceraldehyde-3-phosphate dehydrogenase; glycolysis; intermediate product; metabolites; transmission electron microscopy
- Abstract:
- ... Numerous investigations point to the relation between diabetes and neurodegenerative disorders. Alpha-synuclein is a protein involved in the development of synucleinopathies including Parkinson's disease. In the present work, alpha-synuclein was for the first time modified by the intermediate product of glycolysis, glyceraldehyde-3-phosphate (GA-3-P). The resulting product was compared with the al ...
- DOI:
- 10.1016/j.bbapap.2019.01.003
- https://doi.org/10.1016/j.bbapap.2019.01.003
- Author:
- Abyson Joseph; Valakunja Nagaraja; Ramanathan Natesh
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.9 pp. 757-764
- ISSN:
- 1570-9639
- Subject:
- DNA-directed RNA polymerase; Escherichia coli; Mycobacterium smegmatis; Mycobacterium tuberculosis; circular dichroism spectroscopy; enzyme activity; heat; heat stress; hydrophobicity; messenger RNA; oligomerization; oxidative stress; proteins; stress response; sulfonates; transcription (genetics)
- Abstract:
- ... Gre factors reactivate stalled elongation complexes by enhancing the intrinsic transcript cleavage activity of RNA polymerase. Previous work by us has shown that unlike in Escherichia coli (E.coli), Mycobacterium tuberculosis Gre factor is essential for its survival. Apart from their role in transcription regulation Gre factors have been implicated in stress response. A recent study has shown the ...
- DOI:
- 10.1016/j.bbapap.2019.05.008
- https://doi.org/10.1016/j.bbapap.2019.05.008
- Author:
- Futoshi Ihama; Mami Yamamoto; Chojiro Kojima; Toshimichi Fujiwara; Katsumi Matsuzaki; Yoshihiko Miyata; Masaru Hoshino
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.9 pp. 813-820
- ISSN:
- 1570-9639
- Subject:
- amino acid sequences; circular dichroism spectroscopy; heat-shock protein 90; nuclear magnetic resonance spectroscopy; phosphorylation; phosphotransferases (kinases)
- Abstract:
- ... Cdc37 is a protein kinase-targeting molecular chaperone, which cooperates with Hsp90 to assist the folding, assembly and maturation of various signaling kinases. It consists of three distinct domains: the N-terminal, middle, and C-terminal domain. While the middle domain is an Hsp90-binding domain, the N-terminal domain is recognized as a kinase-interacting domain. The N-terminal domain contains a ...
- DOI:
- 10.1016/j.bbapap.2019.06.007
- https://doi.org/10.1016/j.bbapap.2019.06.007
- Author:
- José L. Neira; Clara Díaz-García; Manuel Prieto; Ana Coutinho
- Source:
- Biochimica et biophysica acta 2019 v.1863 no.4 pp. 760-770
- ISSN:
- 0304-4165
- Subject:
- circular dichroism spectroscopy; dissociation; fluorescence; interferometry; neoplasms; nuclear magnetic resonance spectroscopy; peptides; protein-protein interactions; therapeutics; transcription factors; transcriptional activation; ubiquitination
- Abstract:
- ... The p53, p63 and p73 proteins belong to the p53 family of transcription factors, playing key roles in tumour suppression. The α-splice variant of p73 (p73α) has at its C terminus a sterile alpha motif (SAM); this domain, SAMp73, formed by five helices (α1 to α5), is thought to mediate in protein-protein interactions. The E3-ligase MDM2 binds to p73 at its N terminus transactivation domain (TA), bu ...
- DOI:
- 10.1016/j.bbagen.2019.01.019
- https://doi.org/10.1016/j.bbagen.2019.01.019
16. Thermal unfolding of calreticulin. Structural and thermodynamic characterization of the transition
- Author:
- María B. Decca; Graciela A. Borioli; Edith S. Durand; Alejandro Moreschi; Marta E. Hallak; Guillermo G. Montich
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.3 pp. 175-183
- ISSN:
- 1570-9639
- Subject:
- Fourier transform infrared spectroscopy; calcium; calreticulin; circular dichroism spectroscopy; differential scanning calorimetry; heat; homeostasis; oligomerization; phase transition; protein domains; protein folding; temperature
- Abstract:
- ... Calreticulin (CRT) is a calcium-binding protein that participates in several cellular processes including the control of protein folding and homeostasis of Ca2+. Its folding, stability and functions are strongly controlled by the presence of Ca2+. The oligomerization state of CRT is also relevant for its functions. We studied the thermal transitions of monomers and oligomers of CRT by differential ...
- DOI:
- 10.1016/j.bbapap.2018.12.002
- https://doi.org/10.1016/j.bbapap.2018.12.002
- Author:
- Young-Ho Lee; Yuxi Lin; Sarah J. Cox; Misaki Kinoshita; Bikash R. Sahoo; Magdalena Ivanova; Ayyalusamy Ramamoorthy
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.5 pp. 529-536
- ISSN:
- 1570-9639
- Subject:
- amyloid; cell death; circular dichroism spectroscopy; cytotoxicity; epigallocatechin gallate; fluorescence; humans; insulin; lipids; magnetism; noninsulin-dependent diabetes mellitus; polypeptides; titration; transmission electron microscopy; zinc
- Abstract:
- ... Amyloid aggregation of human islet amyloid polypeptide (hIAPP) is linked to insulin-producing islet cell death in type II diabetes. Previous studies have shown that zinc (Zn(II)) and insulin, co-secreted with hIAPP, have an inhibition effect on hIAPP aggregation. Lipid membranes have also been shown to significantly influence the aggregation kinetics of hIAPP. An increasing number of studies repor ...
- DOI:
- 10.1016/j.bbapap.2018.11.006
- PubMed:
- 30468883
- PubMed Central:
- PMC6450719
- https://doi.org/10.1016/j.bbapap.2018.11.006
18. pH-Dependent fibril maturation of a Pmel17 repeat domain isoform revealed by tryptophan fluorescence
- Author:
- Dexter N. Dean; Jennifer C. Lee
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.10 pp. 961-969
- ISSN:
- 1570-9639
- Subject:
- Raman spectroscopy; alternative splicing; amyloid; circular dichroism spectroscopy; fluorescence; hydrophobicity; melanin; monitoring; pH; polymerization; proteolysis; transmission electron microscopy; tryptophan
- Abstract:
- ... The pre-melanosomal protein (Pmel17) aggregates within melanosomes to form functional amyloid fibrils that facilitate melanin polymerization. The repeat domain (RPT) of Pmel17 fibrillates under strict acidic melanosomal pH. Alternative splicing results in a shortened repeat domain (sRPT), which also forms amyloid fibrils. Here, we explored the effects of pH and protein concentration on sRPT aggreg ...
- DOI:
- 10.1016/j.bbapap.2019.01.012
- PubMed:
- 30716507
- PubMed Central:
- PMC6700743
- https://doi.org/10.1016/j.bbapap.2019.01.012
- Author:
- Kseniia Afitska; Anna Fucikova; Volodymyr V. Shvadchak; Dmytro A. Yushchenko
- Source:
- Biochimica et biophysica acta 2019 v.1867 no.7-8 pp. 701-709
- ISSN:
- 1570-9639
- Subject:
- Parkinson disease; amyloid; circular dichroism spectroscopy; fluorescence; fluorescence emission spectroscopy; light scattering; neurons
- Abstract:
- ... Aggregation of the neuronal protein α-synuclein into amyloid fibrils is a hallmark of Parkinson's disease. The propensity of α-synuclein to aggregate increases with the protein concentration. For the development of efficient inhibitors of α-synuclein aggregation, it is important to know the critical concentration of aggregation (the concentration of monomeric protein, below which the protein does ...
- DOI:
- 10.1016/j.bbapap.2019.05.003
- https://doi.org/10.1016/j.bbapap.2019.05.003